Pseudomonas aeruginosa lytic bacteriophage oKMV
... their narrow host range, there is the need to develop cocktails containing phages that lyse a wider variety of host strains. Any phage cocktail should contain phages that use a diversity of receptors, to ensure an expanded host range and also to suppress the emergence of phage-resistant strains. Hen ...
... their narrow host range, there is the need to develop cocktails containing phages that lyse a wider variety of host strains. Any phage cocktail should contain phages that use a diversity of receptors, to ensure an expanded host range and also to suppress the emergence of phage-resistant strains. Hen ...
Identification of Novel microRNA Regulatory Proteins in Neurons
... it is able to modulate miR-134 immunoblotting using a GFP-specific antibody. activity in general (i.e., by contributing to the miRNext, we decided to validate the positive hits using 134–specific RISC). We found that the knockdown several variations of the reporter assay. First, we of our candidate ...
... it is able to modulate miR-134 immunoblotting using a GFP-specific antibody. activity in general (i.e., by contributing to the miRNext, we decided to validate the positive hits using 134–specific RISC). We found that the knockdown several variations of the reporter assay. First, we of our candidate ...
Isolation of casein from milk
... Structure of casein • Is a phosphoprotein, which has phosphate groups attached to some of the amino acid side chains. Mostly these amino acid are serine and threonine. • casein is a mixture of at least three similar proteins, which differ primarily in molecular weight and amount of phosphorus they ...
... Structure of casein • Is a phosphoprotein, which has phosphate groups attached to some of the amino acid side chains. Mostly these amino acid are serine and threonine. • casein is a mixture of at least three similar proteins, which differ primarily in molecular weight and amount of phosphorus they ...
4th seminar (ELISA, functional)_2016
... Type I (autoimmune) diabetes can be detected by the presence of antibodies against islet-cell specific antigens. Glutamic acid decarboxylase (GAD65), specific antibodies have been found in 70-90% of prediabetic and Type 1 diabetic patients (including approximately 7-10% of adult onset diabetics with ...
... Type I (autoimmune) diabetes can be detected by the presence of antibodies against islet-cell specific antigens. Glutamic acid decarboxylase (GAD65), specific antibodies have been found in 70-90% of prediabetic and Type 1 diabetic patients (including approximately 7-10% of adult onset diabetics with ...
Gel electrophoresis lecture
... – Their net charge therefore is determined by pH of medium in which they are suspended • In a solution with a pH above its isoelectric point, a protein has a net negative charge and migrates towards anode in an electrical field • Below its isoelectric point, protein is positively charged and migrate ...
... – Their net charge therefore is determined by pH of medium in which they are suspended • In a solution with a pH above its isoelectric point, a protein has a net negative charge and migrates towards anode in an electrical field • Below its isoelectric point, protein is positively charged and migrate ...
Supporting information This document contains a comparison
... Among these interactions, H-bonds are the strongest ones. So if we just consider the number of H-bonds observed in these structures we can have a very rough estimate of how much stronger the Arg/PO43- interaction is compared with Glu/ PO43-. Within these structures, the number of Hbonds observed bet ...
... Among these interactions, H-bonds are the strongest ones. So if we just consider the number of H-bonds observed in these structures we can have a very rough estimate of how much stronger the Arg/PO43- interaction is compared with Glu/ PO43-. Within these structures, the number of Hbonds observed bet ...
The Chem. of Carbon
... Nucleic Acids Store & Transmit Hereditary or Genetic Information Every Life Form Uses The Same Nucleic Acids ...
... Nucleic Acids Store & Transmit Hereditary or Genetic Information Every Life Form Uses The Same Nucleic Acids ...
Effect of the addition of CMC on the aggregation behaviour
... that upon g-irradiation SPI and WPI orient themselves in a systematic fashion to favour the electrostatic interaction of CMC with both of them yielding thus, to a more complex three-dimensional network or aggregate with a higher molecular weight. The impact of CMC could be also attributed to its cha ...
... that upon g-irradiation SPI and WPI orient themselves in a systematic fashion to favour the electrostatic interaction of CMC with both of them yielding thus, to a more complex three-dimensional network or aggregate with a higher molecular weight. The impact of CMC could be also attributed to its cha ...
Active transport of antibiotics across the outer membrane of gram
... Fe3+-siderophores. A well-studied example is albomycin, which has structural similarities to the natural substrate ferrichrome; albomycin and ferrichrome are both transported by the FhuA protein. A semisynthetic rifamycin derivative, CGP 4832, is also taken up by the FhuA transport protein, although ...
... Fe3+-siderophores. A well-studied example is albomycin, which has structural similarities to the natural substrate ferrichrome; albomycin and ferrichrome are both transported by the FhuA protein. A semisynthetic rifamycin derivative, CGP 4832, is also taken up by the FhuA transport protein, although ...
Lecture 15, Feb 26
... A polypeptide chain that is folded into its normal, functional conformation is said to be in its native conformation. A polypeptide that is folded improperly so that it cannot function is said to be to be denatured or in a denatured conformation. ...
... A polypeptide chain that is folded into its normal, functional conformation is said to be in its native conformation. A polypeptide that is folded improperly so that it cannot function is said to be to be denatured or in a denatured conformation. ...
Polymers – materials consisting of polymer molecules that consist of
... Polymers – materials consisting of polymer molecules that consist of repeated chemical units (`mers') joined together, like beads on a string. Some polymer molecules contain hundreds or thousands of monomers and are often called macromolecules. Polymers may be natural, such as leather, rubber, cellu ...
... Polymers – materials consisting of polymer molecules that consist of repeated chemical units (`mers') joined together, like beads on a string. Some polymer molecules contain hundreds or thousands of monomers and are often called macromolecules. Polymers may be natural, such as leather, rubber, cellu ...
source document - Enhanced Autoradiography
... In the present report, we describe the application a novel waxed-based system for enhanced autoradiography which is simple to apply and enhances both detection sensitivity and speed. Our studies show that application of EA-WaxTM enhances detection sensitivity to a level which equates with direct det ...
... In the present report, we describe the application a novel waxed-based system for enhanced autoradiography which is simple to apply and enhances both detection sensitivity and speed. Our studies show that application of EA-WaxTM enhances detection sensitivity to a level which equates with direct det ...
FROM GENE TO PROTEIN - Scranton Prep Biology
... termination and explain what enzymes, protein factors, and energy sourcesare neededfor each stage. Explain what determinesthe primary structure of a protein and describehow a polypeptide must be modified before it becomesfully functional. Describe what determines whether a ribosome will be free in t ...
... termination and explain what enzymes, protein factors, and energy sourcesare neededfor each stage. Explain what determinesthe primary structure of a protein and describehow a polypeptide must be modified before it becomesfully functional. Describe what determines whether a ribosome will be free in t ...
introduction
... balanced protein is the main reason for high rate of sickness and death in developing countries (Fontanel, 1972). It is reported that ...
... balanced protein is the main reason for high rate of sickness and death in developing countries (Fontanel, 1972). It is reported that ...
Glycoblocks: a schematic three-dimensional
... clear way is thus of great importance for the understanding of these biological processes. In structural terms, glycosylation sugars glue the protein together via hydrogen bonds, whereas non-covalently bound glycans frequently harness additional stacking interactions. Finding an unobscured molecular ...
... clear way is thus of great importance for the understanding of these biological processes. In structural terms, glycosylation sugars glue the protein together via hydrogen bonds, whereas non-covalently bound glycans frequently harness additional stacking interactions. Finding an unobscured molecular ...
Chapters 5, 7, 11, 17 ppt
... been made by biochemists in protein-folding research This research led to the understanding that it is not a single pathway A funnel shape best describes how an unfolded protein negotiates its way to a low-energy, folded state Numerous routes and intermediates Figure 5.29 The Energy Landscape for ...
... been made by biochemists in protein-folding research This research led to the understanding that it is not a single pathway A funnel shape best describes how an unfolded protein negotiates its way to a low-energy, folded state Numerous routes and intermediates Figure 5.29 The Energy Landscape for ...
msb156484-sup-0001-Appendix
... Subsequent quality assessment and quantification are done by 96-well plate fluorometry and analysis on a Bioanalyzer with Agilent RNA Pico 6000 LabChip kit. The amount of library DNA needed for optimal results in the emulsion-based clonal amplification (emulsion PCR) procedure is determined by emuls ...
... Subsequent quality assessment and quantification are done by 96-well plate fluorometry and analysis on a Bioanalyzer with Agilent RNA Pico 6000 LabChip kit. The amount of library DNA needed for optimal results in the emulsion-based clonal amplification (emulsion PCR) procedure is determined by emuls ...
the glycosylation of alpha-1-acid glycoprotein – structurally complex
... Alpha-1-acid glycoprotein (AGP) is a human plasma protein that belongs to the group of positive acutephase proteins that are produced by the liver. It is also has the ability to bind and carry numerous basic and neutral lipophilic drugs from endogenous (steroid hormones) and exogenous origin. During ...
... Alpha-1-acid glycoprotein (AGP) is a human plasma protein that belongs to the group of positive acutephase proteins that are produced by the liver. It is also has the ability to bind and carry numerous basic and neutral lipophilic drugs from endogenous (steroid hormones) and exogenous origin. During ...
CHAPTER 8 CELLS AND THEIR ENVIRONMENT
... CELL TRANSPORT may divided into 2 major types of process Passive Transport- substances move down or with the concentration gradient Active Transport- substances move up or against their concentration gradient and use cell energy PASSIVE TRANSPORT: substances cross the cell membrane DOWN their concen ...
... CELL TRANSPORT may divided into 2 major types of process Passive Transport- substances move down or with the concentration gradient Active Transport- substances move up or against their concentration gradient and use cell energy PASSIVE TRANSPORT: substances cross the cell membrane DOWN their concen ...
Polyclonal Antibodies to Lamins - Edinburgh Research and Innovation
... Most currently available antibodies to Lamin proteins are made against non‐ideal epitopes. They are either to the Rod domain which is highly conserved, making the antibodies lack specificity among subtypes, or they are to the C terminal globular domain region of the proteins ‐ a region which has ...
... Most currently available antibodies to Lamin proteins are made against non‐ideal epitopes. They are either to the Rod domain which is highly conserved, making the antibodies lack specificity among subtypes, or they are to the C terminal globular domain region of the proteins ‐ a region which has ...
Expressway™ Cell-Free E. coli Expression System
... to allow high-level synthesis of your recombinant protein of interest. At one or several time points after initiating the protein synthesis reaction, the reaction is supplemented with an optimized Feed Buffer containing a proprietary mixture of salts, amino acids, and other substrates that are deple ...
... to allow high-level synthesis of your recombinant protein of interest. At one or several time points after initiating the protein synthesis reaction, the reaction is supplemented with an optimized Feed Buffer containing a proprietary mixture of salts, amino acids, and other substrates that are deple ...
Protein Cross-linkers handbook and selection guide
... Hydrolysis of NHS-Ester competes with the primary amine reaction. Hydrolysis rate increases with increasing pH and occurs more readily in dilute protein solutions. The most widely used cross-linkers that have an amine reactive group are the water insoluble, membrane permeable N-hydroxysuccinimide (N ...
... Hydrolysis of NHS-Ester competes with the primary amine reaction. Hydrolysis rate increases with increasing pH and occurs more readily in dilute protein solutions. The most widely used cross-linkers that have an amine reactive group are the water insoluble, membrane permeable N-hydroxysuccinimide (N ...
Protein adsorption
Adsorption (not to be mistaken for absorption) is the accumulation and adhesion of molecules, atoms, ions, or larger particles to a surface, but without surface penetration occurring. The adsorption of larger biomolecules such as proteins is of high physiological relevance, and as such they adsorb with different mechanisms than their molecular or atomic analogs. Some of the major driving forces behind protein adsorption include: surface energy, intermolecular forces, hydrophobicity, and ionic or electrostatic interaction. By knowing how these factors affect protein adsorption, they can then be manipulated by machining, alloying, and other engineering techniques to select for the most optimal performance in biomedical or physiological applications.