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Linear Polymer
Linear Polymer

... A pendant group on a polymer is a small group of atoms (even a small chain sometimes) that hangs off of the main chain (that is, the backbone of the polymer). ...
Intermolecular Forces
Intermolecular Forces

... Ice is ordered with an open structure to optimize H-bonding. Therefore, ice is less dense than water. In water the H-O bond length is 1.0 Å. The O…H hydrogen bond length is 1.8 Å. Ice has waters arranged in an open, regular hexagon. Each + H points towards a lone pair on O. Ice floats, so it forms ...
〈1055〉 biotechnology- derived articles—peptide mapping
〈1055〉 biotechnology- derived articles—peptide mapping

... Other Parameters—Temperature control of the column is usually necessary to achieve good reproducibility. The flow rates for the mobile phases range from 0.1 to 2.0 mL per minute, and the detection of peptides is performed with a UV detector at 200 to 230 nm. Other methods of detection have been used ...
Package `PPInfer`
Package `PPInfer`

... Description The OCSVM and classical SVM are sequentially used. First, we apply the OCSVM by training a one-class classifier using the data from the known class only and classify the background data. Let n be the number of proteins in the target class. This model is used to predict remaining N-n prot ...
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Proti-Ace Kit - Hampton Research
Proti-Ace Kit - Hampton Research

... 3. Add the protease to the protein crystallization sample. Add 10 ml of the 1 mg/ml Protease Stock solution to 90 ml of 10 mg/ml protein to create a 1:100 w/w dilution. 4. Set the crystallization experiment using the protease:sample mixture. Optimization of In Situ Proteolysis for Crystallization a. ...
Insights from the HuR-interacting transcriptome: ncRNAs, ubiquitin
Insights from the HuR-interacting transcriptome: ncRNAs, ubiquitin

... interacting RNAs. They found that the structures in HuR-positive RNAs may recognize specific fragment which has adenine and uridine bases in a locally less stable RNA duplex. Using Gene Ontology (GO) analysis, the authors showed extensive concentration of Ubiquitin Pathway genes among the HuR-positi ...
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NMR-BIO kits for large proteins labeling
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... group, a carboxyl group, a hydrogen atom, and a variable (R) - grouped according to side chains (nonpolar, polar, acidic, basic) ...
Lehninger Principles of Biochemistry Fourth Edition David L. Nelson
Lehninger Principles of Biochemistry Fourth Edition David L. Nelson

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... Obtain highest quality data Treat Model as Hypothesis Verify Chemical & Biochemical Sense Refer often to the Experimental Map Use Info from Well-Refined Structures – Engh & Huber Bond Lengths & Angles – Implemented in “O” - OOPS • Rotamer Libraries • Main Chain Fragments ...
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... alpha- and beta-carotenes, which are excellent antioxidants. Many scientists believe Americans are not getting adequate carotenes. High Anthocyanin Grape Skin Extract: A potent antioxidant. Reinforces blood vessels, collagen and elastin in the skin. It may be responsible for the French paradox (wher ...
Monte Carlo simulations of peptide–membrane interactions with the
Monte Carlo simulations of peptide–membrane interactions with the

... DGsol is the free energy of transfer of the peptide from the aqueous phase into the membrane. It takes into account electrostatic contributions resulting from changes in solvent polarity, as well as nonpolar effects, both resulting from differences in van der Waals interactions of the peptide with t ...
Polypeptide Synthesis - Fairfax Senior High School
Polypeptide Synthesis - Fairfax Senior High School

...  Events occur in the cell  Introns: intervening sets of nucleotides (junk)  Exons: code for aa, because they are expressed  Once mRNA is processed, where do it go?  What happens to the mRNA molecule after processing ...
Dreze et al, Methods Enzymol 2010
Dreze et al, Methods Enzymol 2010

... activation either with AD expressed alone (i.e., in the absence of any Y fused protein) or even with no AD at all. De novo autoactivators are more difficult to detect than transcription factors and cognate autoactivators. The Y2H system is based on positive growth selections for potentially rare eve ...
EPIgeneousTM Binding Domain Assays
EPIgeneousTM Binding Domain Assays

... streptavidin-acceptor, to detect biotin-peptide. Reader domain interaction with the substrate brings the donor and acceptor dyes into close proximity, enabling FRET to occur upon light excitation. The specific signal at 665 nm is inhibited when a specific compound prevents the reader domain protein ...
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Overcoming stalled translation in human mitochondria
Overcoming stalled translation in human mitochondria

... proteins as members of the human mitochondrial RF family, namely mtRF1, mtRF1a, ICT1, and C12orf65. The first to be identified solely by database searches was mtRF1 (Zhang and Spremulli, 1998). The sequence recognition domains differed from the consensus, supporting the assumption that mtRF1 functione ...
Fractionation of rice glutelin polypeptides using gel filtration
Fractionation of rice glutelin polypeptides using gel filtration

... these components eluted just prior to the 29KD standard, they appearedpresent as free polypeptides and, as such, would be suitable for further characterization and study. Similarly, fraction 4 most likely contained free basic polypeptides, but this fraction was heavily contaminated with the prolamin ...
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Protein adsorption



Adsorption (not to be mistaken for absorption) is the accumulation and adhesion of molecules, atoms, ions, or larger particles to a surface, but without surface penetration occurring. The adsorption of larger biomolecules such as proteins is of high physiological relevance, and as such they adsorb with different mechanisms than their molecular or atomic analogs. Some of the major driving forces behind protein adsorption include: surface energy, intermolecular forces, hydrophobicity, and ionic or electrostatic interaction. By knowing how these factors affect protein adsorption, they can then be manipulated by machining, alloying, and other engineering techniques to select for the most optimal performance in biomedical or physiological applications.
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