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PROTEIN STRUCTURE SIMILARITY CALCULATION AND VISUALIZATION
PROTEIN STRUCTURE SIMILARITY CALCULATION AND VISUALIZATION

... Input to moduleB or visualization module and the output The all by all pairwise similarity calculated in moduleA will be used as input to moduleB. Output should be connectivity graph (as shown in next slide) between all proteins. Each edge must display the similarity value. Preferred output will be ...
keystone apr 2011 - module 1 answers
keystone apr 2011 - module 1 answers

... Part B: There are specialized proteins in the cell membrane that act like pumps for these potassium ions. These pumps use ATP to move sodium ions out of the cell and, in return, move potassium ions into the cell. Because there are different numbers of sodium ions and potassium ions being moved acros ...
16-17 membrane notes
16-17 membrane notes

... = Have both philic and phobic regions • Form a BILAYER with polar heads out/phobic tails in ...
The Scientist : Lab Tools: Close Encounters
The Scientist : Lab Tools: Close Encounters

... information on binary proteinprotein interactions. It can, however, reveal complexes other methods may overlook. "As [the tagged protein] comes off the ribosome, it can assemble with its natural binding partners," explains Superti- Furga. "This allows TAP to capture effects like order of addition, w ...
Purification
Purification

... Methods for protein purification 5. Fractionate by chromatography • several steps are almost always needed • need to assay for amount and purity at each step • need a way to decide when you're finished ...
Chemistry of Life
Chemistry of Life

The Human Cell Poster Introduction
The Human Cell Poster Introduction

... that really do the heavy lifting. While there are around 20,000 genes encoded in our DNA, the total number of proteins is estimated to be many times more—possibly as many as a million*. This is because a single gene might produce multiple variants of a particular protein through, for example, altern ...
Metabolic Pathways
Metabolic Pathways

... The very physiologically important bicarbonate buffer system which we will talk about more than once in this class. Cells produce acids like lactic acid as a metabolic byproduct. The pH of the blood stays in a very narrow normal range (7.35 – 7.45) primarily due to the ability of the bicarbonate buf ...
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... prostheses and other therapies for treating blindness • Optical methods offer certain key advantages over classical electrode recording techniques that are labor intensive, invasive, and yield information about only one or a small number of cells at a time ...
Leukaemia Section t(7;21)(p22;q22) Atlas of Genetics and Cytogenetics in Oncology and Haematology
Leukaemia Section t(7;21)(p22;q22) Atlas of Genetics and Cytogenetics in Oncology and Haematology

... The patient relapsed; however, he is still alive, with a bone marrow graft, 10 years after diagnosis. ...
Affinity Chromatography
Affinity Chromatography

... At slightly basic pH (8-9) most proteins are negatively charged and bind to anionic exchangers. When the ion concentration is raised, first those molecules are eluted which have the lowest number of negative side chains. Highly negatively charged proteins can be eluted only with high ionic strength ...
Influenza A H3N2 (A/X-31) Hemagglutinin / HA Protein (His Tag)
Influenza A H3N2 (A/X-31) Hemagglutinin / HA Protein (His Tag)

... Measured by its ability to agglutinate guinea pig red blood cells. HA titer is 0.2-1μg/mL for 1%GRBC. ...
Advanced Techniques in Molecular Biology
Advanced Techniques in Molecular Biology

... • The signals of low-affinity of protein interactions might not be detected. • There might be a third protein in certain protein-protein interaction. • To choose an appropriate antibody, the target protein needs to be properly predicted. Or there would not be a positive result in Co-IP. • WB can be ...
Chapter 2 Chemical Basis of Life
Chapter 2 Chemical Basis of Life

... Lipids: Three kinds: What 3 elements do they all contain? ( Carbon, hydrogen, and oxygen) ___Fats_______ supply energy, are built from glycerol and three fatty acids. Fatty acids with hydrogen at every position along the carbon chain are saturated; those with one or more double bonds are called __un ...
CG7b-PSSM
CG7b-PSSM

... Computational Genomics, Lecture 6b Partially based on slides by Metsada Pasmanik-Chor ...
Student Misconceptions
Student Misconceptions

... molecules are accurate. However, organic molecules are less static than students imagine. Conveniently drawn as linear, monosaccharides usually form rings in aqueous solutions. There may be considerable rotation around single bonds within organic molecules, unless their structure is stabilized by in ...
Macromolecules Review worksheet
Macromolecules Review worksheet

... Cellulose ...
LABORATORY TESTS THAT REFLECT NUTRITION
LABORATORY TESTS THAT REFLECT NUTRITION

... LABORATORY TESTS THAT REFLECT NUTRITION There are numerous biochemical tests that have nutritional implications. These are a few of the laboratory tests that can be utilized by nurses and dietitians to assess a patient’s nutritional status. Remember, however, that all of these tests provide a wide v ...
Give a brief account of drug protein binding and outline its
Give a brief account of drug protein binding and outline its

... 1995b(13): Give a brief account of drug protein binding and outline its significance General: Drug protein binding refers to the interaction a drug has with proteins. - Can be intravascular, interstitial, or intracellular proteins o Plasma proteins binding is most significant - Interaction is usuall ...
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... Upregulation of GP73 expression by adenovirus infection is specific ...
TRANSCRIPTION & TRANSLATION: From DNA to Protein
TRANSCRIPTION & TRANSLATION: From DNA to Protein

... CAU = Histidine ...
Chemical Bonding File
Chemical Bonding File

... molecule appears when the total energy of the arrangement between atoms has lower energy than the separated atoms. The bound state implies a net attractive force between the atoms. There are several types of chemical bonds are; ...
Biochemistry Review Game
Biochemistry Review Game

... Hydrogen atoms bonded to each other. • Lipids ...
MEMBRANES Fluid mosaic of phopholipid bilayer, cholesterol
MEMBRANES Fluid mosaic of phopholipid bilayer, cholesterol

... Cholesterol (lipid, steroid) providing rigidity , lowers freezing pt. Fluid Mosaic demonstrated by Mouse / Human protein mixing Permeability, Selective Permeability Substances can be moved by exocytosis and endocytosis Small, uncharged molecules generally pass freely through membranes These molecule ...
Chemistry Review - Petal School District
Chemistry Review - Petal School District

...  Water resists temperature change.  Water expands as it freezes. ...
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Protein adsorption



Adsorption (not to be mistaken for absorption) is the accumulation and adhesion of molecules, atoms, ions, or larger particles to a surface, but without surface penetration occurring. The adsorption of larger biomolecules such as proteins is of high physiological relevance, and as such they adsorb with different mechanisms than their molecular or atomic analogs. Some of the major driving forces behind protein adsorption include: surface energy, intermolecular forces, hydrophobicity, and ionic or electrostatic interaction. By knowing how these factors affect protein adsorption, they can then be manipulated by machining, alloying, and other engineering techniques to select for the most optimal performance in biomedical or physiological applications.
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