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Overview of Newborn Screening Molecular Assays
Overview of Newborn Screening Molecular Assays

... Due to high frequency (1 in 176 live births) of MSUD in Mennonite population in WI, mutation analysis for Y438N serves as primary screen for MSUD for Mennonites. CPT1a in Alaskan Innuit & Hutterite populations ...
Paper - IndiaStudyChannel.com
Paper - IndiaStudyChannel.com

... 11. For rough work only the sheets marked “Rough Work” at the end of the Question Booklet be used. 12. The Answer Sheet is designed for computer evaluation. Therefore, if you do not follow the instructions given on the Answer Sheet, it may make evaluation by the computer difficult. Any resultant los ...
Genetic Analysis of RpL38 and RpL5, Two Minute Genes Located in
Genetic Analysis of RpL38 and RpL5, Two Minute Genes Located in

Objectives • Describe the process of DNA transcription. • Explain
Objectives • Describe the process of DNA transcription. • Explain

Foundations of Biology
Foundations of Biology

... of a group of genes (i.e., heat shock proteins) A single gene may be regulated by a number of independent transcription factors (i.e., metallothionein) Eukaryotic regulation does not seem to involve repression To achieve high levels of expression, several different transcription factors binding to d ...
Alternative G-19
Alternative G-19

... nonsense, and frameshift. Include (and label) the coding DNA, template DNA, RNA, and Amino acid sequences that changed. You MUST include the full amino acid sequences from [start] to [stop] even if there are no changes. 2) Rank the 5 mutations you made to your own protein in order from the least aff ...
Identification of a novel testis‐specific gene and its potential roles in
Identification of a novel testis‐specific gene and its potential roles in

... other cell types. Most of these genes also exhibit stagespecific expression during spermatogenesis, which could be considered as spermatogenic cell type-specific since the occurrence of different spermatogenic cell types is also stage-specific during spermatogenesis. Therefore, the spermatogenic cel ...
MyTaq™ HS DNA Polymerase
MyTaq™ HS DNA Polymerase

... genome sequencing projects, the recombinant DNA template is normally purified from the host cell and then amplified by conventional PCR using a thermostable DNA polymerase. Alternately, colony PCR can be performed by adding a single recombinant colony into a DNA polymerase PCR master mix, omitting t ...
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How to search the PDB

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Lecture ten
Lecture ten

... – 1. RNA polymerases differ – only one in prokaryotes; 3 in eukaryotes – 2. transcription factors used by eukaryotes – 3. transcription is terminated differently in prokaryotes vs. eukaryotes • eukaryotes – polyA signal is transcribed prior to termination ...
Analysis of mRNA - quantitation (contd)
Analysis of mRNA - quantitation (contd)

Maurice Godfrey, Ph.D. University of Nebraska Medical Center
Maurice Godfrey, Ph.D. University of Nebraska Medical Center

... “baby” pile. Now each Reebop baby will have 14 chromosomes just like Mom and Dad did. But half will be red and half green, indicating that half came from Mom and half came from Dad. 4. Line up the chromosomes contributed to the baby by the Mom and Dad in pairs of similar size, letter side up. You wi ...
Reebops
Reebops

... Reebop activity in their classroom, but feel a little hesitant about their genetics knowledge. The Reebop activity was originally written for middle and high school students. However, many elementary teachers find that they can use Reebops to teach a variety of lessons to younger students. This outl ...
Prostate cancer cell lines case study on cell cycle map
Prostate cancer cell lines case study on cell cycle map

... Two  interpreta1ons  are  possible  for  the  LNCAP  cells:   -­‐  most  cells  are  expressing  genes  of  the  G1/S  checkpoint.  The  LNCAP  cells  could  try  to  overpass  the   checkpoint  with  less  success  than  DU145  cells. ...
Leukaemia Section t(11;20)(p15;q11) Atlas of Genetics and Cytogenetics in Oncology and Haematology
Leukaemia Section t(11;20)(p15;q11) Atlas of Genetics and Cytogenetics in Oncology and Haematology

... Phenotype/cell stem origin Myeloid, positive for CD34, 33, 13, HLA-DR. ...
Mendelian or qualitative genetics
Mendelian or qualitative genetics

... probability of a specific genotype or phenotype occurring. You can also calculate the phenotypic or genotypic ratios. For example: F2 phenotypes from the split fork example smooth-yellow-red = .75 x .75 x .75 = .43 smooth-yellow-white = .75 x .75 x .25 = .14 smooth-green-red = .75 x .25 x .75 = .14 ...
Y chromosome: Structure and Biological Functions
Y chromosome: Structure and Biological Functions

... (TTY genes) are described as non-coding genes because they do not have an open reading frame 12,2; while the role in spermatogenesis for most of the other genes is still to be elucidated. RBMY1 codes for an RNA binding protein ...
Chapter Three: The Chemistry of Organic Molecules
Chapter Three: The Chemistry of Organic Molecules

... • Quaternary structure- two or more polypeptides combine. • Most enzymes have quaternary structure. • Denaturation of proteins, or the changing of the polypeptide’s shape, can occur due to temperature and pH changes. • Once a protein loses its normal shape, it cannot perform its usual function. ...
Assignment 2
Assignment 2

... team gives the correct answer given that the team members agree? What is the conditional probability that the team gives the correct answer given that the team members disagree? 6. (a) In Octave, simulate 100,000 geometric random variables with parameter p = 0.01 and create a histogram of the result ...
REVERSE GENETICS: USING RNAi TO MAKE PROTEIN KNOCK
REVERSE GENETICS: USING RNAi TO MAKE PROTEIN KNOCK

... Using reverse genetics, one first identifies a gene of interest, and then determines what defect, if any, results when the corresponding protein is missing. This approach may be used to investigate whether a particular protein performs the same functions in one organism as a homologous protein (one ...
Evolutionary rate at the molecular level
Evolutionary rate at the molecular level

... structure of enzymes used in this study does not seem to be known at present, but in thetypical case of esterase-5 the molecular weight was estimated to be about by Narise and H u b b y . I n higher organisms, enzymes with molecular weight of this magnitude seem to be common and usually they are “mu ...
Supplementary Material Short stature in a boy with multiple early
Supplementary Material Short stature in a boy with multiple early

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CHAPTER 10 notes

... TWO forms of a gene controlled by different factors • Alleles: alternative form of single gene • For example: height ...
Characterization of the soil microbial community associated with the
Characterization of the soil microbial community associated with the

... The qPCR primer set was applied to P. chlororaphis pure cultures and for the cloned PueA gene (Group I lipase) from P. chlororaphis. Results were similar for the cloned PueA gene or chromosomal DNA as templates. For all qPCR assays, there was a linear relationship between the log of the plasmid DNA ...
Meiosis Notes November 14, 2012
Meiosis Notes November 14, 2012

... passed from parents to offspring • Tips for making a pedigree 1. Circles are for females 2. Squares are for males 3. Horizontal lines connecting a male and a female represent a marriage 4. Vertical line and brackets connect parent to offspring 5. A shaded circle or square indicates a person has the ...
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Artificial gene synthesis

Artificial gene synthesis is a method in synthetic biology that is used to create artificial genes in the laboratory. Currently based on solid-phase DNA synthesis, it differs from molecular cloning and polymerase chain reaction (PCR) in that the user does not have to begin with preexisting DNA sequences. Therefore, it is possible to make a completely synthetic double-stranded DNA molecule with no apparent limits on either nucleotide sequence or size. The method has been used to generate functional bacterial or yeast chromosomes containing approximately one million base pairs. Recent research also suggests the possibility of creating novel nucleobase pairs in addition to the two base pairs in nature, which could greatly expand the possibility of expanding the genetic code.Synthesis of the first complete gene, a yeast tRNA, was demonstrated by Har Gobind Khorana and coworkers in 1972. Synthesis of the first peptide- and protein-coding genes was performed in the laboratories of Herbert Boyer and Alexander Markham, respectively.Commercial gene synthesis services are now available from numerous companies worldwide, some of which have built their business model around this task. Current gene synthesis approaches are most often based on a combination of organic chemistry and molecular biological techniques and entire genes may be synthesized ""de novo"", without the need for precursor template DNA. Gene synthesis has become an important tool in many fields of recombinant DNA technology including heterologous gene expression, vaccine development, gene therapy and molecular engineering. The synthesis of nucleic acid sequences is often more economical than classical cloning and mutagenesis procedures.
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