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Chapter 3 (part 2)
Chapter 3 (part 2)

... Chapter 3 (part 2) Protein purification and Analysis ...
Isolating the Material of Heredity (Page 568
Isolating the Material of Heredity (Page 568

... called “nuclein”...a “nucleic acid” because of an acidic molecular part. Phoebus Levene did further work on nucleic acids... 1. Isolated 2 types... - have different sugars as part of their structures One has a five carbon sugar molecule in it ( ribose ), Levene called it ribonucleic acid, or RNA. ( ...
PREDICTION OF DELETERIOUS NON­SYNONYMOUS SINGLE NUCLEOTIDE POLYMORPHISMS  (nsSNPs) OF GALC GENE BY COMPUTATIONAL METHOD 
PREDICTION OF DELETERIOUS NON­SYNONYMOUS SINGLE NUCLEOTIDE POLYMORPHISMS  (nsSNPs) OF GALC GENE BY COMPUTATIONAL METHOD 

... corresponding  proteins  called  nonsynonymous  single  nucleotide  polymorphisms  (nsSNPs)  3.These  nsSNPs  occurring  in  protein  coding  region  and  affecting  protein  functions  and  causing  common  diseases  4.  nsSNPs  affect  gene  regulation  by  altering  DNA  and  transcriptional  bin ...
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Bacteria Transformation
Bacteria Transformation

... New Words: Insulin, recombinant DNA, plasmid, gene splicing The first successful insulin preparations came from cows (and later pigs). In the 1980's technology had advanced to the point where we could make human insulin. The technology which made this approach possible was the development of recombi ...
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... heavy polypeptide-like 1), inasmuch as both are involved in translocations with ALK. ...
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... Maes B, De Wolf-Peeters C, Pauwels P, Hagemeijer A, Marynen P. Identification of novel fusion partners of ALK, the anaplastic lymphoma kinase, in anaplastic large-cell lymphoma and inflammatory myofibroblastic tumor. Genes Chromosomes ...
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IBO 2005 Theory Paper - International Biology Olympiad
IBO 2005 Theory Paper - International Biology Olympiad

... (2) The substance(s) has a disulphide bond that is important to its function (3) The substance(s) is stained poorly with Coomassie blue (4) The substance(s) is a protein with molecular mass smaller than 14 kDa. (5) The substance(s) is not resistant to trypsin treatment. ...
Transcription and Translation
Transcription and Translation

... • Only by removing three bases is the reading frame unchanged A: Therefore, a codon must be three bases. ...
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... initiated and terminated by the LAC4 promoter (PLAC4-PB1) and LAC4 transcription terminator (TTLAC4) sequences, respectively. The Saccharomyces cerevisiae ADH1 promoter (PADH1) drives expression of a fungal acetamidase gene (amdS) for the selection of transformants by growth on acetamide-containing ...
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... Monomers are atoms or small molecules that bond together to form more complex structures such as polymers. There are four main types of monomer, including sugars, amino acids, fatty acids, and nucleotides. Each of these monomer types play important roles in the existence and development of life, and ...
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... Besides virus-mediated genedelivery systems, there are several nonviral options for gene delivery. The simplest method is the direct introduction of therapeutic DNA into target cells. This approach is limited in its application because it can be used only with certain tissues and requires large amou ...
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renin-angiotensin system gene polymorphisms and the risk of stroke

... and longitudinally followed up for 7.3+/-1.8 years. +/-G-217A, G-152A, A-20C, G-6A, M235T and T174M polymorphisms of angiotensinogen (AGT) gene, I/D polymorphism of ACE gene, and A1166C polymorphism of AT1R were genotyped. Incident physician-diagnosed ischemic stroke was the outcome measure. At the ...
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A Degenerate ParaHox Gene Cluster in a Degenerate Vertebrate

... An arrayed BAC library of 55,296 clones of average size 118 kb was constructed from 14 ml of whole blood taken from multiple Myxine glutinosa individuals collected at Kristineberg Marine Research Station, Sweden. Library filters and clones are available from Amplicon Express, Pullman, WA (www.genome ...
Chapter 8
Chapter 8

... • It also shows the complimentary bases paired. • The back ribbon-like part is the phosphates and 5 carbon sugar deoxyribose. – Because of their unique structures, adenine can only bond with thymine and cytosine with guanine! ...
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Artificial gene synthesis

Artificial gene synthesis is a method in synthetic biology that is used to create artificial genes in the laboratory. Currently based on solid-phase DNA synthesis, it differs from molecular cloning and polymerase chain reaction (PCR) in that the user does not have to begin with preexisting DNA sequences. Therefore, it is possible to make a completely synthetic double-stranded DNA molecule with no apparent limits on either nucleotide sequence or size. The method has been used to generate functional bacterial or yeast chromosomes containing approximately one million base pairs. Recent research also suggests the possibility of creating novel nucleobase pairs in addition to the two base pairs in nature, which could greatly expand the possibility of expanding the genetic code.Synthesis of the first complete gene, a yeast tRNA, was demonstrated by Har Gobind Khorana and coworkers in 1972. Synthesis of the first peptide- and protein-coding genes was performed in the laboratories of Herbert Boyer and Alexander Markham, respectively.Commercial gene synthesis services are now available from numerous companies worldwide, some of which have built their business model around this task. Current gene synthesis approaches are most often based on a combination of organic chemistry and molecular biological techniques and entire genes may be synthesized ""de novo"", without the need for precursor template DNA. Gene synthesis has become an important tool in many fields of recombinant DNA technology including heterologous gene expression, vaccine development, gene therapy and molecular engineering. The synthesis of nucleic acid sequences is often more economical than classical cloning and mutagenesis procedures.
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