Supplementary Methods (doc 430K)
... RIN-adjusted case-control status. Naïve adjustment may therefore result in many genes being falsely reported as statistically significantly differentially expressed, but we do identify only 100 and 11 genes differentially expressed by OCD and ED respectively at FDR < 5% by Equation 3, which is far f ...
... RIN-adjusted case-control status. Naïve adjustment may therefore result in many genes being falsely reported as statistically significantly differentially expressed, but we do identify only 100 and 11 genes differentially expressed by OCD and ED respectively at FDR < 5% by Equation 3, which is far f ...
Mate-pair, paired ends - molecularevolution.org
... chromatograms) • Base sequence called from flowgrams • Qualities ...
... chromatograms) • Base sequence called from flowgrams • Qualities ...
Supplementary Methods and Results Sequencing bias due to
... transcript will tend to have higher aggregate read counts than a short transcript, even if the two have equal expression, as the long transcript has more opportunities for sequences from fragmented reads to appear. Oshlack and Wakefield (2009) demonstrated empirically that the proportion of differen ...
... transcript will tend to have higher aggregate read counts than a short transcript, even if the two have equal expression, as the long transcript has more opportunities for sequences from fragmented reads to appear. Oshlack and Wakefield (2009) demonstrated empirically that the proportion of differen ...
NATIONAL BRAIN RESEARCH CENTRE(NBRC) NH-8, Manesar-122050, HARYANA
... Mohr’s salt is prepared in warm distilled water by the reaction of (NH4)2SO4 and: ...
... Mohr’s salt is prepared in warm distilled water by the reaction of (NH4)2SO4 and: ...
Foundations of Genetics Mendelism
... chromosomes fail to separate during anaphase-1 or anaphase-2. It leads to formation of gametes with n-1 or n+1 chromosomes. So in humans instead of normal 23 chromosomes some gamete may have 22 or 24 chromosomes. On fertilizing a normal gamete they produce individuals with 45 or 47 chromosomes. Thes ...
... chromosomes fail to separate during anaphase-1 or anaphase-2. It leads to formation of gametes with n-1 or n+1 chromosomes. So in humans instead of normal 23 chromosomes some gamete may have 22 or 24 chromosomes. On fertilizing a normal gamete they produce individuals with 45 or 47 chromosomes. Thes ...
Brief review of Mendelian
... Retardation is more common if the fragile X came from the mother, and is more common in males. Is it sex-linked? The reason for fragility is a much multiplied triplet CGG repeat sequence (usually ~30x, in fragile X 100-1000s of times. Up to ~200 repeats there may be no retardation. But the number o ...
... Retardation is more common if the fragile X came from the mother, and is more common in males. Is it sex-linked? The reason for fragility is a much multiplied triplet CGG repeat sequence (usually ~30x, in fragile X 100-1000s of times. Up to ~200 repeats there may be no retardation. But the number o ...
Supplemental Methods and Figure Legends
... XhoI site was incorporated into the downstream primer for HHT2. The resulting plasmids (pRB633, HHT2; pRB591, HHF2) were used as templates for site-directed mutagenesis using the QuikChange Multi system (Stratagene). P. angusta H3 and H4 each differ from the respective S. cerevisiae proteins at thre ...
... XhoI site was incorporated into the downstream primer for HHT2. The resulting plasmids (pRB633, HHT2; pRB591, HHF2) were used as templates for site-directed mutagenesis using the QuikChange Multi system (Stratagene). P. angusta H3 and H4 each differ from the respective S. cerevisiae proteins at thre ...
Nucleic Acids Research
... ERCC-1 cDNA as a probe. To establish whether this clone encoded a functional ERCC-1 protein the cDNA insert of AcDME was released with EcoRI, subcloned in a SV40 based mammalian expression cartridge (see Materials and Methods) and transfected to CHO 433B cells. The results of this experiment, in whi ...
... ERCC-1 cDNA as a probe. To establish whether this clone encoded a functional ERCC-1 protein the cDNA insert of AcDME was released with EcoRI, subcloned in a SV40 based mammalian expression cartridge (see Materials and Methods) and transfected to CHO 433B cells. The results of this experiment, in whi ...
Southern Blotting DNA Fingerprinting
... Electrophoresis To do Denaturation/DepurinationTo do Blotting Step To do Probing 2 wks done ...
... Electrophoresis To do Denaturation/DepurinationTo do Blotting Step To do Probing 2 wks done ...
Protein Synthsis
... This order is called the reading frame Changing the reading frame completely changes the resulting ...
... This order is called the reading frame Changing the reading frame completely changes the resulting ...
Recombinant DNA cloning technology
... which react with any molecule. • If a protein is injected into a rabbit (or goat, or sheep, etc.) the blood isolated from the injected animal will have antibodies against the injected protein. • Mono-clonal antibodies are produced from cells grown in tissue culture, and can be “made” to have antibod ...
... which react with any molecule. • If a protein is injected into a rabbit (or goat, or sheep, etc.) the blood isolated from the injected animal will have antibodies against the injected protein. • Mono-clonal antibodies are produced from cells grown in tissue culture, and can be “made” to have antibod ...
Genome-wide Dissections of DNA Damage Induced Transcriptional
... dimensionality of the problem co-expression → co-function co-expression → co-regulation • Partition the genes to achieve: Homogeneity: genes inside a cluster show highly similar expression pattern. Separation: genes from different clusters have different expression patterns. ...
... dimensionality of the problem co-expression → co-function co-expression → co-regulation • Partition the genes to achieve: Homogeneity: genes inside a cluster show highly similar expression pattern. Separation: genes from different clusters have different expression patterns. ...
Bacteria and Archaea Generaliza,ons Three Most Common Shapes
... flagella that are structurally and func8onally different from eukaryo8c flagella • In a heterogeneous environment, many bacteria exhibit taxis, the ability to move toward or away from certain s8muli ...
... flagella that are structurally and func8onally different from eukaryo8c flagella • In a heterogeneous environment, many bacteria exhibit taxis, the ability to move toward or away from certain s8muli ...
Virus identification For virus identification samples of nasal
... For virus identification samples of nasal discharge were used. Knowing that this material warrants lower sensitivity than nasopharyngeal aspirate, we chose these samples for compliance reasons since aspiration is associated with some discomfort. The volunteers blew their noses into a cellulose wipe ...
... For virus identification samples of nasal discharge were used. Knowing that this material warrants lower sensitivity than nasopharyngeal aspirate, we chose these samples for compliance reasons since aspiration is associated with some discomfort. The volunteers blew their noses into a cellulose wipe ...
Proximal promoter
... • How much is a TF binding site used – Observed expression of all genes – Predicted site count ...
... • How much is a TF binding site used – Observed expression of all genes – Predicted site count ...
Full Paper - Biotechniques.org
... although not perfectly homologous, still encodes a W-X-X-X-Y amino acid residue. Two of the other segments of the PEX5 gene that encode TPR motifs are fewer than 100bp upstream from the beginning of the 1.2kb sequence reported here. The third is coded by a sequence between bases 840 and 1040 (fig. 3 ...
... although not perfectly homologous, still encodes a W-X-X-X-Y amino acid residue. Two of the other segments of the PEX5 gene that encode TPR motifs are fewer than 100bp upstream from the beginning of the 1.2kb sequence reported here. The third is coded by a sequence between bases 840 and 1040 (fig. 3 ...
Supplementary Material
... Figure S4: Results from nonnegative matrix factorization. (A) Consensus clustering using four algorithms (nsnmf, offset, lee, brunet, see (Gaujoux, R. & Seoighe, C. A flexible R package for nonnegative matrix factorization. BMC Bioinformatics 11, 367 (2010)) for details) showing cophenetic coefficie ...
... Figure S4: Results from nonnegative matrix factorization. (A) Consensus clustering using four algorithms (nsnmf, offset, lee, brunet, see (Gaujoux, R. & Seoighe, C. A flexible R package for nonnegative matrix factorization. BMC Bioinformatics 11, 367 (2010)) for details) showing cophenetic coefficie ...