Characteristics of Phenylacrylic Acid Decarboxylase
... in vivo production of non-natural metabolites in microorganisms via a combination of metabolic engineering, systems biology, and synthetic biology. This process often requires elements of bioprospecting – identifying enzymes capable of produ ...
... in vivo production of non-natural metabolites in microorganisms via a combination of metabolic engineering, systems biology, and synthetic biology. This process often requires elements of bioprospecting – identifying enzymes capable of produ ...
Structure, mechanism and regulation of pyruvate carboxylase
... by rapid oxidation of glucose-derived pyruvate produces a large amount of NADH. This supply of reducing equivalents in turn activates oxidative phosphorylation, resulting in the formation of ATP. Apart from ATP, other nucleotides, including NADPH, are also known to be important coupling factors requ ...
... by rapid oxidation of glucose-derived pyruvate produces a large amount of NADH. This supply of reducing equivalents in turn activates oxidative phosphorylation, resulting in the formation of ATP. Apart from ATP, other nucleotides, including NADPH, are also known to be important coupling factors requ ...
Disposition of Glutathione Conjugates in Rats by a Novel Glutamic
... The metabolites were separated by a gradient solvent system consisting of a mixture of acetonitrile and 0.05% TFA with the flow rate set at 0.7 ml/min. The initial conditions consisted of a mixture of acetonitrile and 0.05% TFA (5:95 v/v) and was maintained for 5 min after the sample was injected. T ...
... The metabolites were separated by a gradient solvent system consisting of a mixture of acetonitrile and 0.05% TFA with the flow rate set at 0.7 ml/min. The initial conditions consisted of a mixture of acetonitrile and 0.05% TFA (5:95 v/v) and was maintained for 5 min after the sample was injected. T ...
Arg305 of Streptomyces l-glutamate oxidase plays a crucial role for
... substrates with the purified enzymes. Effects of pH on activity were examined using Britton-Robinson buffer at a pH range of 4.0–12.0. The activities were measured by the MBTH method at the corresponding pH. 3. Results and discussion 3.1. Conformational simulation of L-glutamate in active site of LGO ...
... substrates with the purified enzymes. Effects of pH on activity were examined using Britton-Robinson buffer at a pH range of 4.0–12.0. The activities were measured by the MBTH method at the corresponding pH. 3. Results and discussion 3.1. Conformational simulation of L-glutamate in active site of LGO ...
Malate Dehydrogenase
... is thought by some to represent the vestige of an ancient organelle that carried out all of the oxygen metabolism of primitive preeukaryotic cells when oxygen entered the atmosphere. Most peroxisomes catalyze the breakdown of fatty acids to acetyl-CoA using a special H2 02-producing enzyme. The acet ...
... is thought by some to represent the vestige of an ancient organelle that carried out all of the oxygen metabolism of primitive preeukaryotic cells when oxygen entered the atmosphere. Most peroxisomes catalyze the breakdown of fatty acids to acetyl-CoA using a special H2 02-producing enzyme. The acet ...
Pyruvate Kinase
... Skeletal muscles ferment glucose to lactate during exercise. Lactate released to the blood may be taken up by other tissues, or by skeletal muscle after exercise, and converted via Lactate Dehydrogenase back to pyruvate, which may be oxidized in Krebs Cycle or (in liver) converted to back to glucose ...
... Skeletal muscles ferment glucose to lactate during exercise. Lactate released to the blood may be taken up by other tissues, or by skeletal muscle after exercise, and converted via Lactate Dehydrogenase back to pyruvate, which may be oxidized in Krebs Cycle or (in liver) converted to back to glucose ...
S17 Cholesterol And Steroids Biosynthesis
... 1- structural lipid present in membrane. 2- precursors of steroidal hormones. - 27 C of cholesterol is derived from acetate. ...
... 1- structural lipid present in membrane. 2- precursors of steroidal hormones. - 27 C of cholesterol is derived from acetate. ...
Unit 6 Vitamins Defining a vitamin Essential
... Rare; Listlessness, fatigue, headache, sleep disturbance, nausea, abdominal distress; Alcoholics at risk Usually in combination with other deficiencies Vitamin B5: _____________________ Exists in free and protein-bound (biocytin) forms; biocytin must be cleaved from protein by biotinidase before bei ...
... Rare; Listlessness, fatigue, headache, sleep disturbance, nausea, abdominal distress; Alcoholics at risk Usually in combination with other deficiencies Vitamin B5: _____________________ Exists in free and protein-bound (biocytin) forms; biocytin must be cleaved from protein by biotinidase before bei ...
The Enterobacteriaceae
... Oxidation-reduction of glucose in the absence of molecular oxygen (anaerobic glycolysis) Energy from hydrolysis of chemical bonds in anaerobic glycolysis captured as high energy phosphate bonds of adenosine triphosphate (ATP) NAD is reduced to NADH2 by accepting electrons during glycolytic conversio ...
... Oxidation-reduction of glucose in the absence of molecular oxygen (anaerobic glycolysis) Energy from hydrolysis of chemical bonds in anaerobic glycolysis captured as high energy phosphate bonds of adenosine triphosphate (ATP) NAD is reduced to NADH2 by accepting electrons during glycolytic conversio ...
14steriod
... 1- structural lipid present in membrane. 2- precursors of steroidal hormones. - 27 C of cholesterol is derived from acetate. ...
... 1- structural lipid present in membrane. 2- precursors of steroidal hormones. - 27 C of cholesterol is derived from acetate. ...
B12 Deficiency
... Methylmalonic acid (functional B12 assay that tells you what your B12 activity was in the past several weeks) Homocysteine (functional test not only for B12 but also becomes abnormal in folic acid or B6 deficiency) ...
... Methylmalonic acid (functional B12 assay that tells you what your B12 activity was in the past several weeks) Homocysteine (functional test not only for B12 but also becomes abnormal in folic acid or B6 deficiency) ...
07 Urea cycle,12. 2012
... glutamate by transamination reactions with αketoglutarate Oxidative deamination of glutamate will ...
... glutamate by transamination reactions with αketoglutarate Oxidative deamination of glutamate will ...
Glucose utilization by Streptomyces griseus
... divided the fermentation into two phases, crescence and senescence. In the former there was uptake of soluble nitrogen, carbon and phosphate into the mycelium, high oxygen demand and rapid utilization of glucose, but very little antibiotic production; in the latter phase, mycelial weight declined, p ...
... divided the fermentation into two phases, crescence and senescence. In the former there was uptake of soluble nitrogen, carbon and phosphate into the mycelium, high oxygen demand and rapid utilization of glucose, but very little antibiotic production; in the latter phase, mycelial weight declined, p ...
Biosynthesis of Isoprenoids
... An interesting feature of the monoterpene synthases is the ability of these enzymes to produce more than one product; for example, pinene synthase from several plant sources produces both α- and β-pinene. The pinenes are among the most common monoterpenes produced by plants and are principal compone ...
... An interesting feature of the monoterpene synthases is the ability of these enzymes to produce more than one product; for example, pinene synthase from several plant sources produces both α- and β-pinene. The pinenes are among the most common monoterpenes produced by plants and are principal compone ...
Cellular Respiration - Spokane Public Schools
... •In the Krebs's cycle, all of Carbons, Hydrogens, and Oxygeng in pyruvate end up as CO2 and H2O •The Krebs's cycle produces 2 ATP's, 8 NADH's, and 2FADH2's per glucose molecule ...
... •In the Krebs's cycle, all of Carbons, Hydrogens, and Oxygeng in pyruvate end up as CO2 and H2O •The Krebs's cycle produces 2 ATP's, 8 NADH's, and 2FADH2's per glucose molecule ...
Cellular Respiration
... •In the Krebs's cycle, all of Carbons, Hydrogens, and Oxygeng in pyruvate end up as CO2 and H2O •The Krebs's cycle produces 2 ATP's, 8 NADH's, and 2FADH2's per glucose molecule ...
... •In the Krebs's cycle, all of Carbons, Hydrogens, and Oxygeng in pyruvate end up as CO2 and H2O •The Krebs's cycle produces 2 ATP's, 8 NADH's, and 2FADH2's per glucose molecule ...
PDF - The Journal of General Physiology
... The coenzyme nicotinamide adenine dinucleotide (NAD+), which plays a crucial role in metabolic redox reactions, participates in a feedback loop with the core clock machinery: NAD+ regulates the transcription of clock genes through the NAD+-dependent deacetylase SIRT1, while expression of the rate-li ...
... The coenzyme nicotinamide adenine dinucleotide (NAD+), which plays a crucial role in metabolic redox reactions, participates in a feedback loop with the core clock machinery: NAD+ regulates the transcription of clock genes through the NAD+-dependent deacetylase SIRT1, while expression of the rate-li ...
Effect of soybean meal particle size on amino acid and energy
... mate 10-d recovery period, all pigs were fed a cornSBM grower diet formulated to 1.00% lysine (total). The experiment was conducted in a 7 × 7 factorial arrangement of treatments in a Latin-square design. Pigs within each of two replicates were allotted to treatment diet sequences before the experim ...
... mate 10-d recovery period, all pigs were fed a cornSBM grower diet formulated to 1.00% lysine (total). The experiment was conducted in a 7 × 7 factorial arrangement of treatments in a Latin-square design. Pigs within each of two replicates were allotted to treatment diet sequences before the experim ...
REDESIGN OF CARNITINE ACETYLTRANSFERASE SPECIFICITY BY PROTEIN ENGINEERING UNIVERSIDAD DE BARCELONA
... proposed to pharmacologically regulate CPT I activity (Price, 2001). Structurally, C75 is a cell-permeable α-methylene-γ-butyrolactone designed to be less reactive and potentially safer than cerulenin, a natural product obtained from the fungus Cephalosporium caerulens. C75 lacks the reactive epoxid ...
... proposed to pharmacologically regulate CPT I activity (Price, 2001). Structurally, C75 is a cell-permeable α-methylene-γ-butyrolactone designed to be less reactive and potentially safer than cerulenin, a natural product obtained from the fungus Cephalosporium caerulens. C75 lacks the reactive epoxid ...
Ox bile, dried
... salts. The bile salts in fresh bile are mainly totally conjugated as peptides formed from bile acid, glycine or taurine. The selective activity of fully conjugated bile acids is less than that of free acids. Deoxycholic acid is the most active of the bile acids. Bacterial enzymes hydrolyse bile conj ...
... salts. The bile salts in fresh bile are mainly totally conjugated as peptides formed from bile acid, glycine or taurine. The selective activity of fully conjugated bile acids is less than that of free acids. Deoxycholic acid is the most active of the bile acids. Bacterial enzymes hydrolyse bile conj ...
Cellular Pathways that Harvest Chemical Energy
... Each reaction in a pathway is catalyzed by a specific enzyme. Metabolic pathways are similar in all organisms, from bacteria to humans. Many metabolic pathways are compartmentalized in eukaryotes, with certain reactions occurring inside an organelle. The operation of each metabolic pathway can be re ...
... Each reaction in a pathway is catalyzed by a specific enzyme. Metabolic pathways are similar in all organisms, from bacteria to humans. Many metabolic pathways are compartmentalized in eukaryotes, with certain reactions occurring inside an organelle. The operation of each metabolic pathway can be re ...
METABOLISM OF POLYSACCHARIDES
... Holoenzymes containing coenzymes (as its non-protein part) which are often vitamin derivatives perform multiple functions. For example, the first enzyme in gluconeogenesis pyruvate carboxylase uses biotin for carboxylation of pyruvate; but the transformation of the pyruvate to acetyl-CoA by pyruvate ...
... Holoenzymes containing coenzymes (as its non-protein part) which are often vitamin derivatives perform multiple functions. For example, the first enzyme in gluconeogenesis pyruvate carboxylase uses biotin for carboxylation of pyruvate; but the transformation of the pyruvate to acetyl-CoA by pyruvate ...
BI25M1
... and oxaloacetate respectively [as we saw in Lecture 2]. For other amino-acids, the conversion involves several steps. ...
... and oxaloacetate respectively [as we saw in Lecture 2]. For other amino-acids, the conversion involves several steps. ...
Document
... One of the most widely occuring steroids, was first isolated in 1770. Contains 8 chiral C atoms, this means that 28 or 256 stereoisomers are possible, but only one of them is cholesterol. ...
... One of the most widely occuring steroids, was first isolated in 1770. Contains 8 chiral C atoms, this means that 28 or 256 stereoisomers are possible, but only one of them is cholesterol. ...
Fatty acid synthesis
Fatty acid synthesis is the creation of fatty acids from acetyl-CoA and malonyl-CoA precursors through action of enzymes called fatty acid synthases. It is an important part of the lipogenesis process, which – together with glycolysis – functions to create fats from blood sugar in living organisms.