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An overview of biochemistry for bioCHEM480
An overview of biochemistry for bioCHEM480

... covalent  bonds  to  both  to  itself  and  these  other  elements,  especially  HNOS  (with  linear,  cyclic  and   branched  skeletons)  together  with  a  limited  number  of  metal  cations  (Na,  K,  Ca,  Mg,  Mn,  Fe,  Co,  Zn, ...
Lecture 39 - Amino Acid Metabolism 2
Lecture 39 - Amino Acid Metabolism 2

... metabolic pathways. These include: 1) three glycolytic pathway intermediates; 3-phosphoglycerate, phosphoenolypyruvate, and pyruvate, 2) two pentose phosphate pathway intermediates; ribose 5phosphate and erythrose 4-phosphate 3) two citrate cycle intermediates; -ketoglutarate and oxaloacetate. ...
Problem Set 2 (multiple choice) Biochemistry 3300 1. What classes
Problem Set 2 (multiple choice) Biochemistry 3300 1. What classes

... citric acid cycle also yields _____ mol of NADH, _____ mol of FADH 2, and _____ mol of ATP (or GTP). a) 2; 2; 2 b) 3; 1; 1 c) 3; 2; 0 d) 4; 1; 1 e) 4; 2; 1 45. Which of the following is correct concerning the metabolic reaction shown below? Pyruvate + NAD+ + CoA-SH ...
Encoding Amino Acids • mRNA codes for amino acids
Encoding Amino Acids • mRNA codes for amino acids

... • mRNA codes for amino acids, which combine to form proteins o But in what way does RNA encode amino acids? • There are 4 RNA nucleotides • Clearly, each nucleotide cannot encode a different amino acid o After all, there are only 4 RNA nucleotides and 20 amino acids • Similarly, suppose we tried usi ...
Molecular cloning and characterization of an acyl
Molecular cloning and characterization of an acyl

... a total volume of 20 μL. Each reaction was performed in triplicate. The quantitative PCRs were performed in the IQ5 Multicolor Realtime PCR Detection System (Bio-Rad, Hercules, CA, USA). All PCR reactions were performed in a 94-well rotor (Bio-Rad) under the following conditions: 5 min at 95°C, 40 c ...
Feodor Lynen - Nobel Lecture
Feodor Lynen - Nobel Lecture

... acid produced by yeast from acetate is formed via citric acid7. Sonderhoff’s experiments with deuterated acetic acid led to another important discovery. In the analysis of the yeast cells themselves, it was found that while the carbohydrate fraction contained only insignificant quantities of deuteri ...
Conductivity of Solutions
Conductivity of Solutions

... Because of the carboxyl group, organic acids are sometimes called “carboxylic acids”. Other organic acids and their sources include: Citric acid – citrus fruit Malic acid – apples Butyric acid – rancid butter Amino acids – protein Nucleic acids – DNA and RNA Ascorbic acid – Vitamin C This is an eno ...
Integration of Metabolism
Integration of Metabolism

... which is coupled with oxidative phosphorylation to generate ATP. Hexose monophosphate shunt: Concerned with the liberation of NADPH, which is utilized for biosynthesis of several compounds, including fatty acids and ribose sugar, which is an essential component of nucleotides. ...
citric acid cycle
citric acid cycle

... 7- In the next reaction, catalyzed by phosphoglycerate kinase, phosphate is transferred from 1,3-bisphosphoglycerate onto ADP, forming ATP (substrate-level phosphorylation) and 3phosphoglycerate. Since two molecules of triose phosphate are formed per molecule of glucose, two molecules of ATP are gen ...
Chemical Composition and antibacterial activity of
Chemical Composition and antibacterial activity of

... indicated the presence of an active compound in this fraction. However, MIC and MBC values for EEP, H-Fr, and P-Fr were higher than that found for the positive control (chlorhexidine 0.12%). This might be explained by the fact that a synthetic pure mono-drug (chlorhexidine) was compared with the fra ...
Secondary structure
Secondary structure

... Peptidyl polymers • A few amino acids in a chain are called a polypeptide. A protein is usually composed of 50 to 400+ amino acids. • Since part of the amino acid is lost during dehydration synthesis, we call the units of a protein amino acid residues. carbonyl carbon ...
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... Transferase deficiency galactosemia is more serious; It is characterized by poor growth in children, mental deficiency and liver damage that may be fatal. ...
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Study Guide Questions Midterm 2

... 13. How   is   fat   absorbed?   Which   vehicles   does   it   use?   How   are   lipids   transported  in  our  body?   14. What  are  the  differences  among  VLDL,  LDL,  IDL,  and  HDL  –  not  just  their   composition  but  what ...
Metabolism: Citric acid cycle
Metabolism: Citric acid cycle

... Aconitase reacts stereo-specifically, which means that the two carbon atoms from acetyl CoA end up at the bottom of isocitrate. In an experiment a carbon atom of pyruvate is labelled with 14C. After how many cycles does this carbon atom first appear in released CO2, and in which step? A. Methyl carb ...
Molecular basis of cardiac efficiency
Molecular basis of cardiac efficiency

... db/db mice [1,18,19]. As both fatty acid oxidation and oxygen consumption occur in mitochondria, the findings of these studies suggest that the basis for impaired cardiac efficiency may be located within mitochondria. Work from our group showed that both ob/ob and db/db hearts pre-perfused with buff ...
MEMBRANE STRUCTURE
MEMBRANE STRUCTURE

... MEMBRANE PROTEINS • INTEGRAL or TRANS – MEMBRANE PROTEINS • LIPID-ANCHORED MEMBRANE PROTEINS • PERIPHERAL MEMBRANE PROTEINS ...
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5 The structure and function of large biological molecules

... Fats serve as insulation in animals, lipid nerve coatings act as electrical insulation, oils and waxes repel water, prevent drying. ...
5. CH 5 PPT The Structure and Function of Macromolecules
5. CH 5 PPT The Structure and Function of Macromolecules

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Biochemistry Final
Biochemistry Final

... other macromolecules (lipids/fatty acids, proteins, and nucleic acids) follow the same guidelines and have similar pathways to supply the body with what it needs. Metabolism is coordinated based on the energy needs (ATP/ADP ratio) of the cells as well as the blood glucose level (glucose, a carbohydr ...
Levels of Organization
Levels of Organization

... • Enzymes are one the most important forms of proteins and will be discussed in detail later in the course. ...
chapter 5 the structure and function of macromolecules
chapter 5 the structure and function of macromolecules

... The covalent bonds connecting monomers in a polymer are disassembled by hydrolysis, a reaction that is effectively the reverse of dehydration. ° In hydrolysis, bonds are broken by the addition of water molecules. A hydrogen atom attaches to one monomer, and a hydroxyl group attaches to the adjacent ...
Krebs Cycle - 2008 BIOCHEM 201
Krebs Cycle - 2008 BIOCHEM 201

... Entrance of acetyl CoA does not ↑ or ↓ intermediates in CAC ...
CITRIC ACID (KREB`S, TCA) CYCLE
CITRIC ACID (KREB`S, TCA) CYCLE

... Acetyl CoA, citrate, and succinylCoA are the end products of individual steps in the citric acid cycle and their accumulation inhibits the step involved in their production. That, of course, results in inhibition of the cycle as a whole. Finally, Ca++ stimulates the citric acid cycle at several poin ...
Chapter 8
Chapter 8

... • Hydrogen Bonds – ...
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Fatty acid synthesis



Fatty acid synthesis is the creation of fatty acids from acetyl-CoA and malonyl-CoA precursors through action of enzymes called fatty acid synthases. It is an important part of the lipogenesis process, which – together with glycolysis – functions to create fats from blood sugar in living organisms.
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