SCIENCE
... 20 times as much was required as of glutamine; and at the optimal level of 20 to 30mM, there was regularly less growth than with glutamine at 1mM. In this case also it is difficult to ascribe the relative inactivity of glutamic acid to the impermeability of the cell, since it was shown to be activel ...
... 20 times as much was required as of glutamine; and at the optimal level of 20 to 30mM, there was regularly less growth than with glutamine at 1mM. In this case also it is difficult to ascribe the relative inactivity of glutamic acid to the impermeability of the cell, since it was shown to be activel ...
BR22, a 26 kDa thyroid transcription factor-1 associated protein
... Institute and the James M. Collins Center for Biomedical Research to J.C. Weissler. ...
... Institute and the James M. Collins Center for Biomedical Research to J.C. Weissler. ...
How are the proteins built up
... • This level of energetic interactions allows stability and flexibility at the same time. Let’s go through the interactions that are important. We will give an overview at this point. Use the link on previous lectures to go into the details of each interaction. 1) Covalent bonds. 50-250 kcal/mole ( ...
... • This level of energetic interactions allows stability and flexibility at the same time. Let’s go through the interactions that are important. We will give an overview at this point. Use the link on previous lectures to go into the details of each interaction. 1) Covalent bonds. 50-250 kcal/mole ( ...
Proteins
... Functions of proteins… • Contractile or motile proteins: Some proteins endow cells and organisms with the ability to contract, change shape, or move about. Actin and myosin function in the contractile system of skeletal muscle and in many other cells. • Structural proteins: Many proteins serve as s ...
... Functions of proteins… • Contractile or motile proteins: Some proteins endow cells and organisms with the ability to contract, change shape, or move about. Actin and myosin function in the contractile system of skeletal muscle and in many other cells. • Structural proteins: Many proteins serve as s ...
Protein: Amino Acids - Resource Sites
... –HCl –Pepsinogen (proenzyme) pepsin (enzyme) –Splits peptide bonds, short proteins ...
... –HCl –Pepsinogen (proenzyme) pepsin (enzyme) –Splits peptide bonds, short proteins ...
Keeping Cells Happy – Topics in Cell Health Maintenance
... Not easily detected, cannot be seen by microscopy Chromosomal aberrations Disruption of nucleic acid synthesis Changes in membrane antigenicity Inhibition of cell proliferation and metabolism Decreased transfection rates Changes in gene expression profiles Cell death ...
... Not easily detected, cannot be seen by microscopy Chromosomal aberrations Disruption of nucleic acid synthesis Changes in membrane antigenicity Inhibition of cell proliferation and metabolism Decreased transfection rates Changes in gene expression profiles Cell death ...
Targets of Oncogenes and Tumor Suppressors: Key for
... kinase). This results in the binding of FAK to the SH2 domain of the Src protooncoprotein followed by recruiting of adapter Grb2 protein, and activation of Ras and MAP kinase cascades (Fig. 2) (see also review by A. G. Tatosyan and O. A. Mizenina in this issue). Increase of cyclin D1 gene expression ...
... kinase). This results in the binding of FAK to the SH2 domain of the Src protooncoprotein followed by recruiting of adapter Grb2 protein, and activation of Ras and MAP kinase cascades (Fig. 2) (see also review by A. G. Tatosyan and O. A. Mizenina in this issue). Increase of cyclin D1 gene expression ...
Biochemistry of RBC Metabolism
... is formed (by bisphosphoglycerate mutase). 2, 3 bisphosphoglycerate decreases affinity of Hb for O2. So, it helps oxyhemoglobin to unload oxygen. ...
... is formed (by bisphosphoglycerate mutase). 2, 3 bisphosphoglycerate decreases affinity of Hb for O2. So, it helps oxyhemoglobin to unload oxygen. ...
Protein Determination - International Dairy Federation
... ISO 8968-4|IDF 020-4:2001 - Milk - Determination of nitrogen content - Part 4: Determination of non-proteinnitrogen content. ...
... ISO 8968-4|IDF 020-4:2001 - Milk - Determination of nitrogen content - Part 4: Determination of non-proteinnitrogen content. ...
CNTF Human, His
... CNTF is a polypeptide hormone whose actions appear to be restricted to the nervous system where it promotes neurotransmitter synthesis and neurite outgrowth in certain neuronal populations. The protein is a potent survival factor for neurons and oligodendrocytes and may be relevant in reducing tissu ...
... CNTF is a polypeptide hormone whose actions appear to be restricted to the nervous system where it promotes neurotransmitter synthesis and neurite outgrowth in certain neuronal populations. The protein is a potent survival factor for neurons and oligodendrocytes and may be relevant in reducing tissu ...
Protein Folding Problem
... protein. If each residue is considered to have just 3 possible conformations the total number of conformations of the protein is 3100. Conformational changes occur on a time scale of 10-13 seconds i.e. the time required to sample all possible conformations would be 3100 x 10-13 seconds which is abou ...
... protein. If each residue is considered to have just 3 possible conformations the total number of conformations of the protein is 3100. Conformational changes occur on a time scale of 10-13 seconds i.e. the time required to sample all possible conformations would be 3100 x 10-13 seconds which is abou ...
Nat 5 Cell Biology Success Criteria
... respiration occurs in the cell cytoplasm. 8. I understand that glucose is broken down into pyruvate in the first stage. 9. I know that if oxygen is available (aerobic conditions) pyruvate is broken down into carbon dioxide and water. 10. I can state that in aerobic conditions there are 38ATP synthes ...
... respiration occurs in the cell cytoplasm. 8. I understand that glucose is broken down into pyruvate in the first stage. 9. I know that if oxygen is available (aerobic conditions) pyruvate is broken down into carbon dioxide and water. 10. I can state that in aerobic conditions there are 38ATP synthes ...
Fermentation Fermentation is an ancient mode of metabolism, and it
... and acetyl phosphate. As a fermentation pathway, it is employed mainly by the heterolactic acid bacteria, which include some species of Lactobacillus and Leuconostoc. In this pathway, glucose-phosphate is oxidized to 6-phosphogluconic acid, which becomes oxidized and decarboxylated to form pentose p ...
... and acetyl phosphate. As a fermentation pathway, it is employed mainly by the heterolactic acid bacteria, which include some species of Lactobacillus and Leuconostoc. In this pathway, glucose-phosphate is oxidized to 6-phosphogluconic acid, which becomes oxidized and decarboxylated to form pentose p ...
Steroid/Thyroid Receptor-like Proteins with
... lysis. Because growth of the normal ("wild-type") cells is inhib ited by adding glucocorticoids, this makes isolation of glucocorticoid-resistant cell variants quite easy. The majority of these resistant cells possess glucocorticoid receptors with structural and functional defects. Four major abnorm ...
... lysis. Because growth of the normal ("wild-type") cells is inhib ited by adding glucocorticoids, this makes isolation of glucocorticoid-resistant cell variants quite easy. The majority of these resistant cells possess glucocorticoid receptors with structural and functional defects. Four major abnorm ...
Chapter 6
... In protostomes as well as deuterostomes, the midline is an embryonic region that functions in patterning the adjacent nervous tissue (Sasai and De Robertis 1997; Menne et al. 1997). The ventral midline in insects (protostomes) is the region from which cells detach to form the ventrally located nerve ...
... In protostomes as well as deuterostomes, the midline is an embryonic region that functions in patterning the adjacent nervous tissue (Sasai and De Robertis 1997; Menne et al. 1997). The ventral midline in insects (protostomes) is the region from which cells detach to form the ventrally located nerve ...
CD spectroscopy
... competing out the binding of other ligands, the affinity for a range of unlabeled ligands can be determined. In this case fluorescence anisotropy – also called polarization - is used for detection, which gives a huge effect since the molecular weight of the probe in complex with the protein becomes ...
... competing out the binding of other ligands, the affinity for a range of unlabeled ligands can be determined. In this case fluorescence anisotropy – also called polarization - is used for detection, which gives a huge effect since the molecular weight of the probe in complex with the protein becomes ...
Extended Project Description
... Crystallography, biophysical and cheminformatics studies for next-generation kinase inhibitor design R. A. Engh, Dept of Chemistry, UiT Background: The human genome contains over 500 homologous protein kinases, which control cellular signalling processes. They have become one of the most important t ...
... Crystallography, biophysical and cheminformatics studies for next-generation kinase inhibitor design R. A. Engh, Dept of Chemistry, UiT Background: The human genome contains over 500 homologous protein kinases, which control cellular signalling processes. They have become one of the most important t ...
Proteomics pathway Most common properties of proteins
... Objetive: Objetive to allow an efficient separation of the greater number of proteins in two dimensions. This procedure is very important and must: must: • Solubilise as many proteins as possible including hydrophobic species • Prevent protein aggregates and hydrophobic interactions. This includes d ...
... Objetive: Objetive to allow an efficient separation of the greater number of proteins in two dimensions. This procedure is very important and must: must: • Solubilise as many proteins as possible including hydrophobic species • Prevent protein aggregates and hydrophobic interactions. This includes d ...
Protein folding
... isomerase) is by far the most common tertiary fold. It is estimated that 10% of all known enzymes have this supersecondary structure. The members of this large family of proteins catalyze very different reactions. Currently, there are 85 enzymes in the TIM database including oxido/reductases, hydrol ...
... isomerase) is by far the most common tertiary fold. It is estimated that 10% of all known enzymes have this supersecondary structure. The members of this large family of proteins catalyze very different reactions. Currently, there are 85 enzymes in the TIM database including oxido/reductases, hydrol ...
Folds
... protein “salting in” results from protein-counter ion binding and the consequently higher net protein charge and solvation; it occurs where the protein has little net charge near its pI primarily by weakly hydrated anions. protein solubility is minimal at the pI (net charge is zero), below or above ...
... protein “salting in” results from protein-counter ion binding and the consequently higher net protein charge and solvation; it occurs where the protein has little net charge near its pI primarily by weakly hydrated anions. protein solubility is minimal at the pI (net charge is zero), below or above ...
Protein Function
... The activity of a GTP-binding protein (also called a GTPase) generally requires the presence of a tightly bound GTP molecule (switch “on”). Hydrolysis of this GTP molecule produces GDP and inorganic phosphate (Pi), and it causes the protein to convert to a different, usually inactive, conformation ( ...
... The activity of a GTP-binding protein (also called a GTPase) generally requires the presence of a tightly bound GTP molecule (switch “on”). Hydrolysis of this GTP molecule produces GDP and inorganic phosphate (Pi), and it causes the protein to convert to a different, usually inactive, conformation ( ...
Paracrine signalling
Paracrine signaling is a form of cell-cell communication in which a cell produces a signal to induce changes in nearby cells, altering the behavior or differentiation of those cells. Signaling molecules known as paracrine factors diffuse over a relatively short distance (local action), as opposed to endocrine factors (hormones which travel considerably longer distances via the circulatory system), juxtacrine interactions, and autocrine signaling. Cells that produce paracrine factors secrete them into the immediate extracellular environment. Factors then travel to nearby cells in which the gradient of factor received determines the outcome. However, the exact distance that paracrine factors can travel is not certain.Although paracrine signaling elicits a diverse array of responses in the induced cells, most paracrine factors utilize a relatively streamlined set of receptors and pathways. In fact, different organs in the body -even between different species - are known to utilize a similar sets of paracrine factors in differential development. The highly conserved receptors and pathways can be organized into four major families based on similar structures: Fibroblast growth factor (FGF) family, Hedgehog family, Wnt family, and TGF-β superfamily. Binding of a paracrine factor to its respective receptor initiates signal transduction cascades, eliciting different responses.