Characterizing transcription factor binding sites using formaldehyde
... was originally identified in a screen for E2F1 target genes and was confirmed as containing a strong in vivo binding site for multiple E2Fs using the standard ChIP assay [1]. In subsequent gel shift analysis, however, ChET8 did not efficiently compete for binding of E2F complexes to a probe containing a ...
... was originally identified in a screen for E2F1 target genes and was confirmed as containing a strong in vivo binding site for multiple E2Fs using the standard ChIP assay [1]. In subsequent gel shift analysis, however, ChET8 did not efficiently compete for binding of E2F complexes to a probe containing a ...
human-physiology-ii-lecture-endomembrane
... •There are two kinds of ER, rough and smooth. •The functions of RER include the synthesis of protein, modification/processing and quality control of newly synthesized proteins. •The SER has functions in several metabolic processes. •It takes part in the synthesis of various lipids , fatty acids and ...
... •There are two kinds of ER, rough and smooth. •The functions of RER include the synthesis of protein, modification/processing and quality control of newly synthesized proteins. •The SER has functions in several metabolic processes. •It takes part in the synthesis of various lipids , fatty acids and ...
IN SILICO SCREENING OF PHYTOCHEMICAL COMPOUNDS TARGETING CHILDHOOD ABSENCE EPILEPSY (CAE)
... transmission within the mammalian central nervous system [1]. They are members of the cys-loop family of ligand-gated ion channels [2]. These transmitter-gated ion channels are assembled as a pentameric complex of subunits which, includes two α subunits, two β subunits, and a γ or δ subunit [3]. GAB ...
... transmission within the mammalian central nervous system [1]. They are members of the cys-loop family of ligand-gated ion channels [2]. These transmitter-gated ion channels are assembled as a pentameric complex of subunits which, includes two α subunits, two β subunits, and a γ or δ subunit [3]. GAB ...
The heterochronic gene lin-29 encodes a zinc finger protein that
... lin-29(n1440). Since these mutant lesions were shown to define at least a portion of the lin-29 gene by intragenic recombination experiments (Papp et al., 1991), genomic DNA from this interval was used to isolate cDNAs (see Materials and Methods). A single class of cDNAs was isolated, which mapped t ...
... lin-29(n1440). Since these mutant lesions were shown to define at least a portion of the lin-29 gene by intragenic recombination experiments (Papp et al., 1991), genomic DNA from this interval was used to isolate cDNAs (see Materials and Methods). A single class of cDNAs was isolated, which mapped t ...
Gene Section ZBTB7A (zinc finger and BTB domain containing 7A)
... in lymphoma, predicts the clinical outcome. ZBTB7A is also implicated in differentiation processes like osteoclastogenesis, chondrogenesis and adipogenesis. Furthermore, ZBTB7A knockdown in mice, results in embryonic lethality due to severe anaemia and impaired cellular differentiation. ZBTB7A is a ...
... in lymphoma, predicts the clinical outcome. ZBTB7A is also implicated in differentiation processes like osteoclastogenesis, chondrogenesis and adipogenesis. Furthermore, ZBTB7A knockdown in mice, results in embryonic lethality due to severe anaemia and impaired cellular differentiation. ZBTB7A is a ...
Protein Synthesis Lab: Day #1
... HASPI Medical Biology Lab 02 Chromosomes are simply portions of DNA wound up and organized into a form that makes it easier for cells to find the directions, or gene, that it needs to make a specific protein. Different organisms have a different number of chromosomes depending on the amount of DNA, ...
... HASPI Medical Biology Lab 02 Chromosomes are simply portions of DNA wound up and organized into a form that makes it easier for cells to find the directions, or gene, that it needs to make a specific protein. Different organisms have a different number of chromosomes depending on the amount of DNA, ...
Autophagy in the Eukaryotic Cell - CiteSeerX
... and travels along with this protein to the vacuole, where it is finally degraded by vacuolar proteases. The association of prApe1 with Cvt19 promotes the inclusion of both proteins into the forming Cvt vesicles (98), but it is not clear yet if the formation of this complex is the event that triggers ...
... and travels along with this protein to the vacuole, where it is finally degraded by vacuolar proteases. The association of prApe1 with Cvt19 promotes the inclusion of both proteins into the forming Cvt vesicles (98), but it is not clear yet if the formation of this complex is the event that triggers ...
Print
... Although only a few dozen such substrates have been identified (110), in vitro studies suggest that the number of cellular caspase substrates may ultimately prove to be much larger, perhaps several hundred to one thousand (Z. Li, L. Zhong, and D. Bredesen, unpublished results). In any case, however, ...
... Although only a few dozen such substrates have been identified (110), in vitro studies suggest that the number of cellular caspase substrates may ultimately prove to be much larger, perhaps several hundred to one thousand (Z. Li, L. Zhong, and D. Bredesen, unpublished results). In any case, however, ...
3 Amino acids and crude protein - DLG
... • gaseous losses of N from faeces and urine • result often representative only for short periods of time or weight • feed intake pre-determined ...
... • gaseous losses of N from faeces and urine • result often representative only for short periods of time or weight • feed intake pre-determined ...
Conserved functions of retinoblastoma proteins: From purple retina
... cell cycle [59]. The RB-family members are differentially expressed during mouse development and their ability to arrest the cell cycle can be cell-type specific [60], which is also a confirmation of distinct cellular and developmental functions (for more details see the following reviews: [61,62]). ...
... cell cycle [59]. The RB-family members are differentially expressed during mouse development and their ability to arrest the cell cycle can be cell-type specific [60], which is also a confirmation of distinct cellular and developmental functions (for more details see the following reviews: [61,62]). ...
LOF1 and Interacting Transcription Factors in Plant Development
... This open access poster is brought to you by The Cupola: Scholarship at Gettysburg College. It has been accepted for inclusion by an authorized administrator of The Cupola. For more information, please contact [email protected]. ...
... This open access poster is brought to you by The Cupola: Scholarship at Gettysburg College. It has been accepted for inclusion by an authorized administrator of The Cupola. For more information, please contact [email protected]. ...
3D-structure of bacterial ribosomes, the machines that make
... most proteins don’t During production many proteins can only obtain correct folding if assisted by other proteins (chaperones) during production in living cells If large quantities of a specific chaperone-dependent protein is produced in a cell, it may become misfolded. This represents a very seriou ...
... most proteins don’t During production many proteins can only obtain correct folding if assisted by other proteins (chaperones) during production in living cells If large quantities of a specific chaperone-dependent protein is produced in a cell, it may become misfolded. This represents a very seriou ...
BIOL 230 Introductory Cell Biology
... Evaluation and analysis of the living cell and its components. Examines cell structures and metabolism as they relate to cell function and reproduction. (Recommended for all life science and medical science majors.) . One or more field trips may be required. Extra supplies may be required. 4. STUDEN ...
... Evaluation and analysis of the living cell and its components. Examines cell structures and metabolism as they relate to cell function and reproduction. (Recommended for all life science and medical science majors.) . One or more field trips may be required. Extra supplies may be required. 4. STUDEN ...
3. Proteins Classification (2017)
... Can be converted into soluble gelatins The major proteins of by boiling contain large amounts of ...
... Can be converted into soluble gelatins The major proteins of by boiling contain large amounts of ...
3-D Structure of proteins
... 3. likelyhood of errors greater for long polypeptides. Therefore more efficient to synthesize several smaller subunits 4. 3D-structure changes when proteins bind ligands. Critical step in biological activity of many proteins (i.e regulatory function) ...
... 3. likelyhood of errors greater for long polypeptides. Therefore more efficient to synthesize several smaller subunits 4. 3D-structure changes when proteins bind ligands. Critical step in biological activity of many proteins (i.e regulatory function) ...
In Silico Analysis: Annotations about Structural and Functional
... Domains of unknown functions (DUFs) are a big set of protein families within the Pfam database that includes proteins of unknown function. In the absence of functional information, proteins are classified into different families based on conserved amino acid sequences and are potentially functionall ...
... Domains of unknown functions (DUFs) are a big set of protein families within the Pfam database that includes proteins of unknown function. In the absence of functional information, proteins are classified into different families based on conserved amino acid sequences and are potentially functionall ...
PKC in limb development
... 1990). FGFR1 mutant mice, in which a tyrosine critical for the interaction between FGFR and PLCγ is mutated, have defects in patterning of the anteroposterior axis (Partanen et al., 1998). These data provide direct evidence for a role of the FGF/PLCγ/PKC signal transduction pathway in development. A ...
... 1990). FGFR1 mutant mice, in which a tyrosine critical for the interaction between FGFR and PLCγ is mutated, have defects in patterning of the anteroposterior axis (Partanen et al., 1998). These data provide direct evidence for a role of the FGF/PLCγ/PKC signal transduction pathway in development. A ...
Lecture 5: Major Nutrient Groups
... primary: the sequence of AA’s forming the protein secondary: forces generated by the close proximity of one AA residue to another (e.g., helix design or pleated sheet)(i.e., certain amino acids can form bonds with others, if close enough, cysteine) tertiary: bending of one AA chain due to attrac ...
... primary: the sequence of AA’s forming the protein secondary: forces generated by the close proximity of one AA residue to another (e.g., helix design or pleated sheet)(i.e., certain amino acids can form bonds with others, if close enough, cysteine) tertiary: bending of one AA chain due to attrac ...
function finders
... -- A film of mucins can protect cells from micro-organisms and particles that may damage the cell (see image below of intestinal mucin-producing cells). -- To date 19 human mucin genes have been identified. -- There are two classes of human mucin proteins – membrane-bound and secreted. Mucin-1 is cl ...
... -- A film of mucins can protect cells from micro-organisms and particles that may damage the cell (see image below of intestinal mucin-producing cells). -- To date 19 human mucin genes have been identified. -- There are two classes of human mucin proteins – membrane-bound and secreted. Mucin-1 is cl ...
![An FGF4-FRS2[alpha]-Cdx2 Axis in Trophoblast Stem Cells Induces](http://s1.studyres.com/store/data/004612399_1-b568e9f7ef7ac5f7304ea5d975b0dd52-300x300.png)
An FGF4-FRS2[alpha]-Cdx2 Axis in Trophoblast Stem Cells Induces
... role in the activation of PI-3 kinases and a secondary role in the activation of ERK [16]. Frs2a begins to be expressed in the early stages of development. Compared with its weak expression in the epiblast, Frs2a is strongly expressed in ExE and TS cells [15, 19]. We have previously shown that Frs2a ...
... role in the activation of PI-3 kinases and a secondary role in the activation of ERK [16]. Frs2a begins to be expressed in the early stages of development. Compared with its weak expression in the epiblast, Frs2a is strongly expressed in ExE and TS cells [15, 19]. We have previously shown that Frs2a ...
INOTROPIC AGENTS - Dr Ted Williams
... Forms a 1:1 complex with plasminogen and induces a conformational change new conformation gives the complex plasmin-like catalytic activity can cleave other plasminogen molecules to plasmin PROBLEMS: SK is a foreign protein and antibodies are produced. requires a loading dose to overcome antibodies ...
... Forms a 1:1 complex with plasminogen and induces a conformational change new conformation gives the complex plasmin-like catalytic activity can cleave other plasminogen molecules to plasmin PROBLEMS: SK is a foreign protein and antibodies are produced. requires a loading dose to overcome antibodies ...
SCUBE3 (Signal Peptide-CUB-EGF Domain
... FIGURE 1. Scube3 knockdown causes defective myogenic differentiation and reduced FGF8 signaling. A, knockdown of Scube3 mRNA expression in murine C2C12 myoblasts. C2C12 myoblasts were infected with a lentiviral vector to generate a stable clone expressing an shRNA control or shRNA specific for mouse ...
... FIGURE 1. Scube3 knockdown causes defective myogenic differentiation and reduced FGF8 signaling. A, knockdown of Scube3 mRNA expression in murine C2C12 myoblasts. C2C12 myoblasts were infected with a lentiviral vector to generate a stable clone expressing an shRNA control or shRNA specific for mouse ...
Protein Nanocages - Nanyang Technological University
... enough to each other at the interface and are accessible, they will repel each other due to the same charge upon arriving at acidic pH environment. The result is the destabilization of the interaction between the subunits and the disassembly, facilitating molecular release from the lumen of the prot ...
... enough to each other at the interface and are accessible, they will repel each other due to the same charge upon arriving at acidic pH environment. The result is the destabilization of the interaction between the subunits and the disassembly, facilitating molecular release from the lumen of the prot ...
Paracrine signalling
Paracrine signaling is a form of cell-cell communication in which a cell produces a signal to induce changes in nearby cells, altering the behavior or differentiation of those cells. Signaling molecules known as paracrine factors diffuse over a relatively short distance (local action), as opposed to endocrine factors (hormones which travel considerably longer distances via the circulatory system), juxtacrine interactions, and autocrine signaling. Cells that produce paracrine factors secrete them into the immediate extracellular environment. Factors then travel to nearby cells in which the gradient of factor received determines the outcome. However, the exact distance that paracrine factors can travel is not certain.Although paracrine signaling elicits a diverse array of responses in the induced cells, most paracrine factors utilize a relatively streamlined set of receptors and pathways. In fact, different organs in the body -even between different species - are known to utilize a similar sets of paracrine factors in differential development. The highly conserved receptors and pathways can be organized into four major families based on similar structures: Fibroblast growth factor (FGF) family, Hedgehog family, Wnt family, and TGF-β superfamily. Binding of a paracrine factor to its respective receptor initiates signal transduction cascades, eliciting different responses.