Identification of fungal oxaloacetate hydrolyase within the
... Two ORFs directly adjacent to ORFs encoding OAHlike class proteins were used to query fungal genomes encoding one or more OAH-like class proteins by blastX and if a single very highly significant hit resulted (E-value < 1 E 210), this was considered to be the likely ortholog of the query. When two g ...
... Two ORFs directly adjacent to ORFs encoding OAHlike class proteins were used to query fungal genomes encoding one or more OAH-like class proteins by blastX and if a single very highly significant hit resulted (E-value < 1 E 210), this was considered to be the likely ortholog of the query. When two g ...
Feed Ingredients and Feeds for Channel Catfish
... extraction process. Levels of soybean meal up to 50 percent have been used in commercial catfish feeds without detrimental effects. In recent years, its use has been reduced somewhat because of increasing cost. Cottonseed meal, solvent-extracted, is obtained by finely grinding the flakes that remain ...
... extraction process. Levels of soybean meal up to 50 percent have been used in commercial catfish feeds without detrimental effects. In recent years, its use has been reduced somewhat because of increasing cost. Cottonseed meal, solvent-extracted, is obtained by finely grinding the flakes that remain ...
The transcriptional regulator CprK detects chlorination by combining
... (each with distinct DNA-binding affinities) are populated to significant levels [20]. By contrast, it has been proposed that CprK exhibits either extreme positive or no cooperativity, both effectively leading to an equilibrium between unbound and fully bound CprK [16]. In the latter case, this presu ...
... (each with distinct DNA-binding affinities) are populated to significant levels [20]. By contrast, it has been proposed that CprK exhibits either extreme positive or no cooperativity, both effectively leading to an equilibrium between unbound and fully bound CprK [16]. In the latter case, this presu ...
Passenger Protein Determines Translocation Versus Retention in
... protocol (the reagents and buffers were supplied by the manufacturer). For proteolysis, aliquots of translated product were treated with 1 mg/ml of proteinase K (PK) at room temperature for 15 minutes. Proteolysis was terminated by the addition of phenylmethylsulfonyl fluoride to a final concentrati ...
... protocol (the reagents and buffers were supplied by the manufacturer). For proteolysis, aliquots of translated product were treated with 1 mg/ml of proteinase K (PK) at room temperature for 15 minutes. Proteolysis was terminated by the addition of phenylmethylsulfonyl fluoride to a final concentrati ...
3.2 Breeding of corn line DAS-40278-9
... Characterisation of Novel Protein Corn DAS-40278-9 expresses one novel protein, AAD-1. It is expressed in leaves, pollen, roots, grain and forage. The average content in mature grain is 4.8 µg/g dry weight (range 1.07-9.10 µg/g). Several studies were undertaken to confirm the identity and physicoche ...
... Characterisation of Novel Protein Corn DAS-40278-9 expresses one novel protein, AAD-1. It is expressed in leaves, pollen, roots, grain and forage. The average content in mature grain is 4.8 µg/g dry weight (range 1.07-9.10 µg/g). Several studies were undertaken to confirm the identity and physicoche ...
The Principles of Balancing Diets for Amino Acids and Their Impact
... Many nutritionists have questioned the possibility of histidine (His) as a potential limiting AA. However, to the author’s knowledge, His has only been identified as first limiting when grass silage and barley and oat diets were fed, with or without feather meal as a sole or primary source of supple ...
... Many nutritionists have questioned the possibility of histidine (His) as a potential limiting AA. However, to the author’s knowledge, His has only been identified as first limiting when grass silage and barley and oat diets were fed, with or without feather meal as a sole or primary source of supple ...
HIV-1 Vpr-chloramphenicol acetyltransferase fusion proteins
... HIV virion-incorporation of different Vpr-CAT fusion proteins To delineate the minimal region of Vpr required for efficient virion incorporation of Vpr-fusion proteins, we constructed a series of Vpr-CAT chimeric proteins by fusing different fragments of Vpr to the N- or C-terminus of CAT (Figure 1a ...
... HIV virion-incorporation of different Vpr-CAT fusion proteins To delineate the minimal region of Vpr required for efficient virion incorporation of Vpr-fusion proteins, we constructed a series of Vpr-CAT chimeric proteins by fusing different fragments of Vpr to the N- or C-terminus of CAT (Figure 1a ...
Accurate Prediction of Contact Numbers for Multi
... Traditionally, prediction of the contact numbers for soluble proteins is treated as a two-state (higher or lower than the average contact number) or three-state (much higher, much lower, or close to the average contact number) classification problem.14−16 However, the applicability of classification a ...
... Traditionally, prediction of the contact numbers for soluble proteins is treated as a two-state (higher or lower than the average contact number) or three-state (much higher, much lower, or close to the average contact number) classification problem.14−16 However, the applicability of classification a ...
Leukaemia Section Chronic myelogenous leukaemia (CML) Atlas of Genetics and Cytogenetics
... Multipotent (and primitive: CD34+, DR-) progenitor: t(9;22) is found in any myeloid progenitor and in Blymphocytes progenitors, but, most often, not in the Tcells. Epidemiology Annual incidence: 10/106 (from 1/106 in childhood to 30/106 after 60 yrs); median age: 30-60 yrs; sex ratio: 1.2M/1F. Clini ...
... Multipotent (and primitive: CD34+, DR-) progenitor: t(9;22) is found in any myeloid progenitor and in Blymphocytes progenitors, but, most often, not in the Tcells. Epidemiology Annual incidence: 10/106 (from 1/106 in childhood to 30/106 after 60 yrs); median age: 30-60 yrs; sex ratio: 1.2M/1F. Clini ...
ProRule: a new database containing functional
... All the other information provided by the rule is conditional, depending on the presence of mandatory residues at some precise positions within the domain. A feature line for a predicted disulfide bond will only be created if cysteine residues are found at the two positions connected by the bond. He ...
... All the other information provided by the rule is conditional, depending on the presence of mandatory residues at some precise positions within the domain. A feature line for a predicted disulfide bond will only be created if cysteine residues are found at the two positions connected by the bond. He ...
Spinalin, a new glycine- and histidine
... The pET-15b vector encoding spinalin19K was transformed into E. coli BL21(DE3) cells (Novagen, Madison, WI 53711) and expression of spinalin19K was induced by IPTG. Cells were lysed by sonication and the debris removed by centrifugation (13,000 g for 20 minutes). To purify histidine-tagged spinalin1 ...
... The pET-15b vector encoding spinalin19K was transformed into E. coli BL21(DE3) cells (Novagen, Madison, WI 53711) and expression of spinalin19K was induced by IPTG. Cells were lysed by sonication and the debris removed by centrifugation (13,000 g for 20 minutes). To purify histidine-tagged spinalin1 ...
Molecular Cloning and Expression of a 2-Arylpropionyl
... standard methods as described by the manufacturer (Qiagen). Samples were resolved on a 10% SDS gel and transferred onto nitrocellulose membranes (Schleicher & Schuell). All subsequent steps were conducted at room temperature. Filters were blocked for 1 hr with TBS-T (20 mM Tris base, 137 mM NaCl, HC ...
... standard methods as described by the manufacturer (Qiagen). Samples were resolved on a 10% SDS gel and transferred onto nitrocellulose membranes (Schleicher & Schuell). All subsequent steps were conducted at room temperature. Filters were blocked for 1 hr with TBS-T (20 mM Tris base, 137 mM NaCl, HC ...
Reducing Rice Seed Storage Protein Accumulation Leads to
... visible on a stained gel (Fig. 1A and Supplemental Fig. S1). On the other hand, levels of Glb-1 and 13kD prolamins significantly increased (Fig. 1A). GluB-less seeds had decreased levels of GluB-4, GluB-2 and GluB-1, whereas levels of Glb-1 and 13kD prolamins increased appreciably (Fig. 1A and Supp ...
... visible on a stained gel (Fig. 1A and Supplemental Fig. S1). On the other hand, levels of Glb-1 and 13kD prolamins significantly increased (Fig. 1A). GluB-less seeds had decreased levels of GluB-4, GluB-2 and GluB-1, whereas levels of Glb-1 and 13kD prolamins increased appreciably (Fig. 1A and Supp ...
Characterization of Phosphorylation Sites from the Activation Loop
... expressed in E. coli. Purified GST-fusion ERK1 was assayed in vitro with wild-type (WT) MEK1 and a super active mutant MEK1-ΔEE (with deletion of its N-terminal auto-inhibition domain and double Glu mutation at two activatory phosphosites). ERK1’s activity was tested by its ability to phosphorylate ...
... expressed in E. coli. Purified GST-fusion ERK1 was assayed in vitro with wild-type (WT) MEK1 and a super active mutant MEK1-ΔEE (with deletion of its N-terminal auto-inhibition domain and double Glu mutation at two activatory phosphosites). ERK1’s activity was tested by its ability to phosphorylate ...
... acids, valuable edible alga. Oral vaccines or drugs produced with D.salina can be taken directly even without purification, so that the cost of production may be reduced markedly. • The bioreactor system according to the present research may be used to produce drugs or vaccines for humans and/or ani ...
From Sequence to Structure
... Hydrophobic amino-acid residues engage in van der Waals interactions only. Their tendency to avoid contact with water and pack against each other is the basis for the hydrophobic effect. Alanine and leucine are strong helix-favoring residues, while proline is rarely found in helices because its back ...
... Hydrophobic amino-acid residues engage in van der Waals interactions only. Their tendency to avoid contact with water and pack against each other is the basis for the hydrophobic effect. Alanine and leucine are strong helix-favoring residues, while proline is rarely found in helices because its back ...
1 Chapter 1 Introduction 1.1 – Introduction Proteins are
... selenium and tellurium have been developed for incorporation into proteins. Importantly, these amino acid derivatives are recognized by the protein synthesis machinery and can, therefore, be incorporated in vivo. These heavy atoms allow crystallographers to more easily locate discrete positions in a ...
... selenium and tellurium have been developed for incorporation into proteins. Importantly, these amino acid derivatives are recognized by the protein synthesis machinery and can, therefore, be incorporated in vivo. These heavy atoms allow crystallographers to more easily locate discrete positions in a ...
proteinstructure
... Dmin ~ 2.5Å tells us where backbone and most side-chain atoms are Dmin ~ 1.2Å: all protein atoms, most solvent, some disordered atoms ...
... Dmin ~ 2.5Å tells us where backbone and most side-chain atoms are Dmin ~ 1.2Å: all protein atoms, most solvent, some disordered atoms ...
Biological Networks Underlying Abiotic Stress Tolerance in
... comprehensive reviews on proteomics of abiotic stresses in crop plants [5,6] as well as more specialized reviews dedicated to specific stress factors, crops or cellular fractions such as reviews on salinity proteomics [7–9], subcellular proteomics of crop plants exposed to stress [10], proteomics of ...
... comprehensive reviews on proteomics of abiotic stresses in crop plants [5,6] as well as more specialized reviews dedicated to specific stress factors, crops or cellular fractions such as reviews on salinity proteomics [7–9], subcellular proteomics of crop plants exposed to stress [10], proteomics of ...
Determination of De Novo Synthesized Amino Acids in Cellular
... proline was largely decreased. Apparently, hypoosmotic conditions stimulate the synthesis of proteins or peptides with a high proline content. The results show that already after 2 h of incubation with [U-13C]glucose there is a pronounced flux of 13C label into the cellular proteins, which is usuall ...
... proline was largely decreased. Apparently, hypoosmotic conditions stimulate the synthesis of proteins or peptides with a high proline content. The results show that already after 2 h of incubation with [U-13C]glucose there is a pronounced flux of 13C label into the cellular proteins, which is usuall ...
Docking with GOLD Tutorial
... orientation of the amide groups in Asn and Gln side chains protein side chains can move ? Have I checked that: there is crystallographic evidence for the position of long and surface exposed side chains, such as Arg and Lys ? there are no unwanted water or buffer molecules in my active site ? ...
... orientation of the amide groups in Asn and Gln side chains protein side chains can move ? Have I checked that: there is crystallographic evidence for the position of long and surface exposed side chains, such as Arg and Lys ? there are no unwanted water or buffer molecules in my active site ? ...
Chapter 6 Pichia pastoris
... ammonia-lyase. The product of the C4H reaction, 4-coumaric acid, is then activated to its CoA thioester by 4-coumarate: CoA ligase, and the 4-coumaroyl CoA is then funneled into branched pathways leading to a wide array of phenolic metabolites, including lignin, flavonoids and coumarins (Yamamura et ...
... ammonia-lyase. The product of the C4H reaction, 4-coumaric acid, is then activated to its CoA thioester by 4-coumarate: CoA ligase, and the 4-coumaroyl CoA is then funneled into branched pathways leading to a wide array of phenolic metabolites, including lignin, flavonoids and coumarins (Yamamura et ...
Novel surface layer protein genes in Bacillus
... (Ishiguro et al., 1981; Lewis et al., 1987; Mesnage et al., 2001; Mignot et al., 2001), an attachment structure for high-molecular-mass extracellular proteins such as amylases (Matuschek et al., 1994; Egelseer et al., 1995, 1996), an adhesin with affinity for human epithelial cells and fibronectin ( ...
... (Ishiguro et al., 1981; Lewis et al., 1987; Mesnage et al., 2001; Mignot et al., 2001), an attachment structure for high-molecular-mass extracellular proteins such as amylases (Matuschek et al., 1994; Egelseer et al., 1995, 1996), an adhesin with affinity for human epithelial cells and fibronectin ( ...
Gene Section ABL (v-abl Abelson murine leukemia viral oncogene homolog 1)
... Probe 1132H12 on a case of CML with t(9/22). Note the splitting of the probe, evident also on interphase nuclei - Courtesy Mariano Rocchi, Resources for Molecular Cytogenetics . ...
... Probe 1132H12 on a case of CML with t(9/22). Note the splitting of the probe, evident also on interphase nuclei - Courtesy Mariano Rocchi, Resources for Molecular Cytogenetics . ...
Protein purification
Protein purification is a series of processes intended to isolate one or a few proteins from a complex mixture, usually cells, tissues or whole organisms. Protein purification is vital for the characterization of the function, structure and interactions of the protein of interest. The purification process may separate the protein and non-protein parts of the mixture, and finally separate the desired protein from all other proteins. Separation of one protein from all others is typically the most laborious aspect of protein purification. Separation steps usually exploit differences in protein size, physico-chemical properties, binding affinity and biological activity. The pure result may be termed protein isolate.The methods used in protein purification can roughly be divided into analytical and preparative methods. The distinction is not exact, but the deciding factor is the amount of protein that can practically be purified with that method. Analytical methods aim to detect and identify a protein in a mixture, whereas preparative methods aim to produce large quantities of the protein for other purposes, such as structural biology or industrial use. In general, the preparative methods can be used in analytical applications, but not the other way around.