![S1. Comparison of complex functions in MCL-GO](http://s1.studyres.com/store/data/010551281_1-3c570e2441b153caedcbbeb67b8a854f-300x300.png)
S1. Comparison of complex functions in MCL-GO
... For each superfamily we determined the functional coherence of proteins containing a member of that superfamily and compared it to random groups of proteins of the same size as the superfamily. Functional coherence was calculated as the average GOSS score between each pair of proteins, either contai ...
... For each superfamily we determined the functional coherence of proteins containing a member of that superfamily and compared it to random groups of proteins of the same size as the superfamily. Functional coherence was calculated as the average GOSS score between each pair of proteins, either contai ...
Regulation of cellular homoeostasis by reversible lysine acetylation
... (transactivator of transcription)-interactive protein 60 kDa], MOZ (monocytic leukaemic zinc-finger protein), HBO1 (histone acetyltransferase binding to Orc1), MORF (MOZ-related factor) and MOF (male absent on first). These proteins have a wide range of acetylation substrates, and regulate pathways ...
... (transactivator of transcription)-interactive protein 60 kDa], MOZ (monocytic leukaemic zinc-finger protein), HBO1 (histone acetyltransferase binding to Orc1), MORF (MOZ-related factor) and MOF (male absent on first). These proteins have a wide range of acetylation substrates, and regulate pathways ...
... to eliminate short-circuiting by preventing the uncoupled processes (that is, substrate translocation or ATP hydrolysis) from occurring individually in their energetically favorable directions. The challenge to biochemists has been to define the molecular details of a mechanism that goes beyond the ...
The trans-Golgi network GRIP-domain proteins form α
... To determine whether the four mammalian GRIP proteins interact to form oligomers, a series of co-immunoprecipitation experiments was performed using epitope-tagged constructs. Mycand FLAG-tagged full-length constructs were generated for each of the four TGN golgins as described in the Experimental s ...
... To determine whether the four mammalian GRIP proteins interact to form oligomers, a series of co-immunoprecipitation experiments was performed using epitope-tagged constructs. Mycand FLAG-tagged full-length constructs were generated for each of the four TGN golgins as described in the Experimental s ...
CSCI 474 Lab 4a : inferring the effects of mutations Spring 2017
... (try to) infer the effect of a mutation using only the sequence of amino acids in a protein. Such tools have varying degrees of accuracy for the reason that the effect of a mutation is dependent on how the amino acid substitution/deletion/insertion affects the 3D structure of the protein, and not ju ...
... (try to) infer the effect of a mutation using only the sequence of amino acids in a protein. Such tools have varying degrees of accuracy for the reason that the effect of a mutation is dependent on how the amino acid substitution/deletion/insertion affects the 3D structure of the protein, and not ju ...
Molecular Interaction of PICKI and ATXN3
... SCA3 is one of the nine known polyQ diseases which would be inherited in an autosomal dominantly. Exact disease mechanism of SCA3 is not fully understood and there is currently no known therapy to affect disease progression. SCA3 is solely caused by CAG expansion of ATXN3, a deubiquitinase with a un ...
... SCA3 is one of the nine known polyQ diseases which would be inherited in an autosomal dominantly. Exact disease mechanism of SCA3 is not fully understood and there is currently no known therapy to affect disease progression. SCA3 is solely caused by CAG expansion of ATXN3, a deubiquitinase with a un ...
Targeted Quantitation of HMGB1 Protein by label
... includes a number of well-established options like MS-based methods, coupled to protein (or peptide) separation and bioinformatics analysis allow the achievement of protein identification and quantification [1]. The identification of protein PTMs is very relevant because they can cause significant c ...
... includes a number of well-established options like MS-based methods, coupled to protein (or peptide) separation and bioinformatics analysis allow the achievement of protein identification and quantification [1]. The identification of protein PTMs is very relevant because they can cause significant c ...
Comparison of Trypsin Immobilization Techniques With or Without a
... proteomics, fast enzymatic digestions and efficient analysis techniques like capillary electrophoresis (CE), liquid chromatography (HPLC) and mass spectrometry (MS) are essential. Immobilized enzymes, defined as enzymes with restricted mobility, offer technical and economical advantages over soluble ...
... proteomics, fast enzymatic digestions and efficient analysis techniques like capillary electrophoresis (CE), liquid chromatography (HPLC) and mass spectrometry (MS) are essential. Immobilized enzymes, defined as enzymes with restricted mobility, offer technical and economical advantages over soluble ...
L-Tyrosine - Douglas Labs
... Deutsch SI, Rosse RB, Schwartz BL, Banay-Schwartz M, McCarthy MF, Johri SK. L-tyrosine pharmacotherapy of schizophrenia: preliminary data. Clin Neuropharmacol 1994;17:53-62. Elwes RD, Crewes H, Chesterman LP, et al. Treatment of narcolepsy with L-tyrosine: double-blind placebocontrolled trial. Lance ...
... Deutsch SI, Rosse RB, Schwartz BL, Banay-Schwartz M, McCarthy MF, Johri SK. L-tyrosine pharmacotherapy of schizophrenia: preliminary data. Clin Neuropharmacol 1994;17:53-62. Elwes RD, Crewes H, Chesterman LP, et al. Treatment of narcolepsy with L-tyrosine: double-blind placebocontrolled trial. Lance ...
Genes Section DEK (DEK oncogene) Atlas of Genetics and Cytogenetics
... This work is licensed under a Creative Commons Attribution-Non commercial-No Derivative Works 2.0 France Licence. © 1998 Atlas of Genetics and Cytogenetics in Oncology and Haematology ...
... This work is licensed under a Creative Commons Attribution-Non commercial-No Derivative Works 2.0 France Licence. © 1998 Atlas of Genetics and Cytogenetics in Oncology and Haematology ...
Histidine protonation and the activation of viral fusion proteins
... pK a of histidine in water (pK a = 6). This strongly suggests that the process is triggered by the protonation of one or more histidine residues. Viral fusion proteins have been separated into two classes based on structural and functional properties [5]. An analysis of HA (haemagglutinin) (class I) ...
... pK a of histidine in water (pK a = 6). This strongly suggests that the process is triggered by the protonation of one or more histidine residues. Viral fusion proteins have been separated into two classes based on structural and functional properties [5]. An analysis of HA (haemagglutinin) (class I) ...
2.3 Carbon-Based Molecules
... 2.3 Carbon-Based Molecules • Many carbon-based molecules are made of many small subunits bonded together. – Monomers are the individual subunits. – Polymers are made of many monomers. ...
... 2.3 Carbon-Based Molecules • Many carbon-based molecules are made of many small subunits bonded together. – Monomers are the individual subunits. – Polymers are made of many monomers. ...
NC Exam Questions - Rosshall Academy
... (ii) How many hydrogen atoms are present in a molecule of phenylalanine? (1) (b) Phenylalanine and alanine can react to form the dipeptide shown. ...
... (ii) How many hydrogen atoms are present in a molecule of phenylalanine? (1) (b) Phenylalanine and alanine can react to form the dipeptide shown. ...
The Possible DNA-binding Nature of the Regulatory Proteins
... positions 8-9-10 (leucine, glycine, valine) and methionine at position 5. The last of these residues does not appear to be unacceptable, as the trp apo-repressor contains a lysine at this position. However, at position 15 only hydrophobic residues have previously been observed and here the gerE sequ ...
... positions 8-9-10 (leucine, glycine, valine) and methionine at position 5. The last of these residues does not appear to be unacceptable, as the trp apo-repressor contains a lysine at this position. However, at position 15 only hydrophobic residues have previously been observed and here the gerE sequ ...
Document
... Cholera toxin (CT) of Vibrio cholera is a Type II secreted toxin. CT is an AB5 toxin, where the A domain (~27.4 kDa) consists of two components, CT-A1 and CT-A2 and the B domain (~58 kDa) is a homopentameric protein complex CT-A1 ADP- ribosylates the Gα- subunit of the heterotrimeric protein, Gs. CT ...
... Cholera toxin (CT) of Vibrio cholera is a Type II secreted toxin. CT is an AB5 toxin, where the A domain (~27.4 kDa) consists of two components, CT-A1 and CT-A2 and the B domain (~58 kDa) is a homopentameric protein complex CT-A1 ADP- ribosylates the Gα- subunit of the heterotrimeric protein, Gs. CT ...
Glycosylation of Proteins - Structure, Function and Analysis
... Glycosylation is one of the most widely observed, and structurally diverse, forms of post translational modification (PTM) of proteins. Animal, plant, fungal and bacterial cell systems all have the ability to glycosylate proteins and, whilst similarities do exist between these groups, there are also ...
... Glycosylation is one of the most widely observed, and structurally diverse, forms of post translational modification (PTM) of proteins. Animal, plant, fungal and bacterial cell systems all have the ability to glycosylate proteins and, whilst similarities do exist between these groups, there are also ...
TALKING POINT The origin of the eukaryotic cell
... sequences be reconciled? Because some of the proteins suggest the same topology as the rRNA sequences, should we prefer that particular inference and ignore the others? It is important to exclude two possibilities that could lead to incorrect phylogenies before considering other models to explain th ...
... sequences be reconciled? Because some of the proteins suggest the same topology as the rRNA sequences, should we prefer that particular inference and ignore the others? It is important to exclude two possibilities that could lead to incorrect phylogenies before considering other models to explain th ...
Identification of genes and proteins involved in the
... concentration of this metalloid corresponding roughly to onehalf the MIC were then explored using 2D-electrophoresis. Bacteria were grown in CDM medium [10] to stationary phase and after cell disruption, proteins were separated on 2D SDS-PAGE gels, silver stained, and analyzed by mass spectrometry ( ...
... concentration of this metalloid corresponding roughly to onehalf the MIC were then explored using 2D-electrophoresis. Bacteria were grown in CDM medium [10] to stationary phase and after cell disruption, proteins were separated on 2D SDS-PAGE gels, silver stained, and analyzed by mass spectrometry ( ...
Summary from Organic Chemistry Packet:
... • Recognize the terms cis-, trans- isomers – Unsaturated molecules – Orientation around the double bond ...
... • Recognize the terms cis-, trans- isomers – Unsaturated molecules – Orientation around the double bond ...
Mass Spectrometry
... genome of the organism into proteins • Theoretically cut the proteins into peptides with the same protease (ex.Trypsin: K or R) • Calculate the absolute masses of the peptides from each protein • the masses of the peptides of the unknown protein vs the theoretical peptide masses of each protein enco ...
... genome of the organism into proteins • Theoretically cut the proteins into peptides with the same protease (ex.Trypsin: K or R) • Calculate the absolute masses of the peptides from each protein • the masses of the peptides of the unknown protein vs the theoretical peptide masses of each protein enco ...
Amino Acids
... mimic some of its effects, can cause serious physical and mental problems, may be prescribed to treat diseases such as cancer and AIDS, and – are abused by athletes to enhance performance. ...
... mimic some of its effects, can cause serious physical and mental problems, may be prescribed to treat diseases such as cancer and AIDS, and – are abused by athletes to enhance performance. ...
Using storage organelles for the accumulation and encapsulation of
... transplastomic tobacco, transient expression in tobacco using Agrobacterium tumefaciens, plant viruses or hybrids thereof, hydroponic tobacco root secretion, hairy root cultures, and tobacco suspension cells [5]. This diversity has resulted in a large number of independent molecular farming enterpri ...
... transplastomic tobacco, transient expression in tobacco using Agrobacterium tumefaciens, plant viruses or hybrids thereof, hydroponic tobacco root secretion, hairy root cultures, and tobacco suspension cells [5]. This diversity has resulted in a large number of independent molecular farming enterpri ...
Cyclol
![](https://commons.wikimedia.org/wiki/Special:FilePath/Cyclol_reaction.png?width=300)
The cyclol hypothesis is the first structural model of a folded, globular protein. It was developed by Dorothy Wrinch in the late 1930s, and was based on three assumptions. Firstly, the hypothesis assumes that two peptide groups can be crosslinked by a cyclol reaction (Figure 1); these crosslinks are covalent analogs of non-covalent hydrogen bonds between peptide groups. These reactions have been observed in the ergopeptides and other compounds. Secondly, it assumes that, under some conditions, amino acids will naturally make the maximum possible number of cyclol crosslinks, resulting in cyclol molecules (Figure 2) and cyclol fabrics (Figure 3). These cyclol molecules and fabrics have never been observed. Finally, the hypothesis assumes that globular proteins have a tertiary structure corresponding to Platonic solids and semiregular polyhedra formed of cyclol fabrics with no free edges. Such ""closed cyclol"" molecules have not been observed either.Although later data demonstrated that this original model for the structure of globular proteins needed to be amended, several elements of the cyclol model were verified, such as the cyclol reaction itself and the hypothesis that hydrophobic interactions are chiefly responsible for protein folding. The cyclol hypothesis stimulated many scientists to research questions in protein structure and chemistry, and was a precursor of the more accurate models hypothesized for the DNA double helix and protein secondary structure. The proposal and testing of the cyclol model also provides an excellent illustration of empirical falsifiability acting as part of the scientific method.