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AtCHIP functions as an E3 ubiquitin ligase of protein phosphatase
... 2001; Jiang et al., 2001). AtCHIP is encoded by a single-copy gene in the Arabidopsis genome and it is the only U-box protein found in plants that also contains TPRs (Yan et al., 2003). Like animal CHIP proteins, AtCHIP has E3 ubiquitin ligase activity in vitro. AtCHIP transcripts were upregulated b ...
... 2001; Jiang et al., 2001). AtCHIP is encoded by a single-copy gene in the Arabidopsis genome and it is the only U-box protein found in plants that also contains TPRs (Yan et al., 2003). Like animal CHIP proteins, AtCHIP has E3 ubiquitin ligase activity in vitro. AtCHIP transcripts were upregulated b ...
Dynamin and the Actin Cytoskeleton Cooperatively Regulate
... These observations prompted us to carry out a more systematic comparison between BAR domain- and FCH domain-containing proteins. Sequence comparisons and structural predictions suggested that the FCH domain, together with the CC region that typically follows this domain, define a protein module simi ...
... These observations prompted us to carry out a more systematic comparison between BAR domain- and FCH domain-containing proteins. Sequence comparisons and structural predictions suggested that the FCH domain, together with the CC region that typically follows this domain, define a protein module simi ...
Thermostability of the human respiratory syncytial virus fusion
... neither sequence nor predicted structural features with the 3These authors contributed equally to this work. ...
... neither sequence nor predicted structural features with the 3These authors contributed equally to this work. ...
selection of antigens for antibody-based proteomics
... The human genome is predicted to contain ~20,500 protein-coding genes. The encoded proteins are the key players in the body, but the functions and localizations of most proteins are still unknown. Antibody-based proteomics has great potential for exploration of the protein complement of the human ge ...
... The human genome is predicted to contain ~20,500 protein-coding genes. The encoded proteins are the key players in the body, but the functions and localizations of most proteins are still unknown. Antibody-based proteomics has great potential for exploration of the protein complement of the human ge ...
Mitochondrial quality control by the ubiquitin
... operate at mitochondria [10–12,14,15]. In this system, matrix or inner membrane proteins are retrotranslocated to the outer membrane for ubiquitination and targeting to the proteasome. Several observations support the existence of such a system. Proteins associated with various mitochondrial compart ...
... operate at mitochondria [10–12,14,15]. In this system, matrix or inner membrane proteins are retrotranslocated to the outer membrane for ubiquitination and targeting to the proteasome. Several observations support the existence of such a system. Proteins associated with various mitochondrial compart ...
Protein Cross-linkers handbook and selection guide
... not available, they can be generated by either the reduction of disulfides (-S-S-) with reducing agents such as mercaptoethylamine; or by modifying lysine ε-amines with Traut’s reagent or SATA. If disulfide bond reduction is used, then excess reducing agent must be removed before reaction with sulfh ...
... not available, they can be generated by either the reduction of disulfides (-S-S-) with reducing agents such as mercaptoethylamine; or by modifying lysine ε-amines with Traut’s reagent or SATA. If disulfide bond reduction is used, then excess reducing agent must be removed before reaction with sulfh ...
Chapter 5A Lecture
... The heme group of myoglobin (and hemoglobin) is sequestered within a crevice, or pocket, in the protein (Refer to Fig. 4-16). The burying of the heme prevents a reaction that would occur with free heme in solution in which one O2 binds to two sandwiched heme groups and oxidizes iron to Fe3+. In myog ...
... The heme group of myoglobin (and hemoglobin) is sequestered within a crevice, or pocket, in the protein (Refer to Fig. 4-16). The burying of the heme prevents a reaction that would occur with free heme in solution in which one O2 binds to two sandwiched heme groups and oxidizes iron to Fe3+. In myog ...
Cellular Disulfide Bond Formation in Bioactive Peptides and
... Cysteines, sulfhydryl-containing amino acids, which are located an appropriate distance or next to one another within a polypeptide chain, will form a disulfide bond through their oxidisable thiol groups. This bond will impart a fold in the chain of the protein or bend in its structure. Disulfide bo ...
... Cysteines, sulfhydryl-containing amino acids, which are located an appropriate distance or next to one another within a polypeptide chain, will form a disulfide bond through their oxidisable thiol groups. This bond will impart a fold in the chain of the protein or bend in its structure. Disulfide bo ...
Scavenging of 14-3-3 proteins reveals their involvement in the cell
... of KATP channels (Yuan et al., 2003) implies that steric masking by the other subunit is not sufficient to explain inactivation of all the Arg-based signals present in the complex. Previous studies using soluble protein-A fusion proteins and reporter membrane proteins have shown that the C-terminal ...
... of KATP channels (Yuan et al., 2003) implies that steric masking by the other subunit is not sufficient to explain inactivation of all the Arg-based signals present in the complex. Previous studies using soluble protein-A fusion proteins and reporter membrane proteins have shown that the C-terminal ...
Soy protein isolate
... improve both product purity and yield. These depend on many different parameters, including alkalinity, extraction time, temperature, the ratio of water to soy meal, heat treatment and agitation. ...
... improve both product purity and yield. These depend on many different parameters, including alkalinity, extraction time, temperature, the ratio of water to soy meal, heat treatment and agitation. ...
Chemical Shift Restraints Tools and Methods
... Chemical shifts values are indirectly related to geometry (SHIFTX, CamShift) NOEs have long-range information Chemical shifts are local NOEs are redundant There is only one chemical shift per atom Clear quality control (number of assigned NOEs, NOEs violation) ...
... Chemical shifts values are indirectly related to geometry (SHIFTX, CamShift) NOEs have long-range information Chemical shifts are local NOEs are redundant There is only one chemical shift per atom Clear quality control (number of assigned NOEs, NOEs violation) ...
Biological membranes - Essays in Biochemistry
... through the membrane. Phospholipids can diffuse relatively quickly in the leaflet of the bilayer in which they are located. A phospholipid can travel around the perimeter of a red blood cell in around 12 s, or move the length of a bacterial cell within 1 s. Phospholipids can also spin around on thei ...
... through the membrane. Phospholipids can diffuse relatively quickly in the leaflet of the bilayer in which they are located. A phospholipid can travel around the perimeter of a red blood cell in around 12 s, or move the length of a bacterial cell within 1 s. Phospholipids can also spin around on thei ...
Protein Data Bank Contents Guide: Atomic Coordinate Entry
... There are no character/column format changes in the records in this section; however, the definition of a PDB HET group is revised owing to the change in nomenclature for the standard deoxyribonucleotides as described in the following section. HET HET records are used to describe non-standard residu ...
... There are no character/column format changes in the records in this section; however, the definition of a PDB HET group is revised owing to the change in nomenclature for the standard deoxyribonucleotides as described in the following section. HET HET records are used to describe non-standard residu ...
Protein Engineering in the Development of
... residues lie in a plane along the length of a helix; the hydrophobic nature of the plane leads to the formation of multistranded coiled coils. The motif’s name reflects the predominance of leucine residues at the a and d positions. The aggregation or assembly of two or more domains into a coiled-coil ...
... residues lie in a plane along the length of a helix; the hydrophobic nature of the plane leads to the formation of multistranded coiled coils. The motif’s name reflects the predominance of leucine residues at the a and d positions. The aggregation or assembly of two or more domains into a coiled-coil ...
Fast Protein Folding in the Hydrophobic
... model abstracts the dominant force of protein folding: the hydrophobic interaction. The protein is modeled as a chain of amino acids of length n i.e., nonpolar) which are of two types: H (hydrophobic, i.e., polar). Although this model is and P (hydrophilic, a simplification of more complex protein f ...
... model abstracts the dominant force of protein folding: the hydrophobic interaction. The protein is modeled as a chain of amino acids of length n i.e., nonpolar) which are of two types: H (hydrophobic, i.e., polar). Although this model is and P (hydrophilic, a simplification of more complex protein f ...
bioinformatics - Noble Research Lab
... a subset of the interactions, and it has been estimated that for the organism with the most complete interaction network, namely yeast, only about half of the complete ‘interactome’ has been discovered (von Mering et al., 2002). In view of the very small overlap between interactions discovered by va ...
... a subset of the interactions, and it has been estimated that for the organism with the most complete interaction network, namely yeast, only about half of the complete ‘interactome’ has been discovered (von Mering et al., 2002). In view of the very small overlap between interactions discovered by va ...
GPCR–G fusion proteins
... The b2AR coupled to GsaL, but not the b2AR coupled to GsaS possesses the properties of a constitutively active GPCR. These differences can be explained by the lower GDPaffinity of GsaL compared to the GDP-affinity of GsaS, i.e. GsaL is more often GDP-free than GsaS and, therefore, more often availab ...
... The b2AR coupled to GsaL, but not the b2AR coupled to GsaS possesses the properties of a constitutively active GPCR. These differences can be explained by the lower GDPaffinity of GsaL compared to the GDP-affinity of GsaS, i.e. GsaL is more often GDP-free than GsaS and, therefore, more often availab ...
Biosynthesis of Protein or Translation
... The amino acids have no direct affinity for mRNA, so tRNA act as an adapter molecule, which recognizes an amino acid on one end and its corresponding codon on the other, is required for translation. ...
... The amino acids have no direct affinity for mRNA, so tRNA act as an adapter molecule, which recognizes an amino acid on one end and its corresponding codon on the other, is required for translation. ...
PRIONS THE INFECTIOUS PROTEINS
... Phospholipase A2 Inhibitors prevent prion replication. Platelet-activating Factor Antagonists also inhibits prion ...
... Phospholipase A2 Inhibitors prevent prion replication. Platelet-activating Factor Antagonists also inhibits prion ...
Magnificent molecules
... breakdown of the proteins in the corpse, and two of them have such horrible odours that they have been named putrescine (after the process of putrefaction) and cadaverine (after the Latin word for a corpse: cadaver). Putrescine and cadaverine are ...
... breakdown of the proteins in the corpse, and two of them have such horrible odours that they have been named putrescine (after the process of putrefaction) and cadaverine (after the Latin word for a corpse: cadaver). Putrescine and cadaverine are ...
MagneHis™ Protein Purification System Technical Manual
... Two methods for lysis of bacterial cells using the FastBreak™ Cell Lysis Reagent, 10X, are presented in this section. In Method 1, cells are pelleted, and then the FastBreak™ Cell Lysis Reagent, 10X, diluted to 1X concentration, is added to the cell pellet with DNase I. Alternatively, FastBreak™ Cel ...
... Two methods for lysis of bacterial cells using the FastBreak™ Cell Lysis Reagent, 10X, are presented in this section. In Method 1, cells are pelleted, and then the FastBreak™ Cell Lysis Reagent, 10X, diluted to 1X concentration, is added to the cell pellet with DNase I. Alternatively, FastBreak™ Cel ...
161021 NGF revised Manuscript with figs
... aggregation involving reversible self-association is an increasingly recognised problem affecting the ...
... aggregation involving reversible self-association is an increasingly recognised problem affecting the ...
Cyclol
![](https://commons.wikimedia.org/wiki/Special:FilePath/Cyclol_reaction.png?width=300)
The cyclol hypothesis is the first structural model of a folded, globular protein. It was developed by Dorothy Wrinch in the late 1930s, and was based on three assumptions. Firstly, the hypothesis assumes that two peptide groups can be crosslinked by a cyclol reaction (Figure 1); these crosslinks are covalent analogs of non-covalent hydrogen bonds between peptide groups. These reactions have been observed in the ergopeptides and other compounds. Secondly, it assumes that, under some conditions, amino acids will naturally make the maximum possible number of cyclol crosslinks, resulting in cyclol molecules (Figure 2) and cyclol fabrics (Figure 3). These cyclol molecules and fabrics have never been observed. Finally, the hypothesis assumes that globular proteins have a tertiary structure corresponding to Platonic solids and semiregular polyhedra formed of cyclol fabrics with no free edges. Such ""closed cyclol"" molecules have not been observed either.Although later data demonstrated that this original model for the structure of globular proteins needed to be amended, several elements of the cyclol model were verified, such as the cyclol reaction itself and the hypothesis that hydrophobic interactions are chiefly responsible for protein folding. The cyclol hypothesis stimulated many scientists to research questions in protein structure and chemistry, and was a precursor of the more accurate models hypothesized for the DNA double helix and protein secondary structure. The proposal and testing of the cyclol model also provides an excellent illustration of empirical falsifiability acting as part of the scientific method.