![Lecture_9_2005](http://s1.studyres.com/store/data/008314807_1-f3b05812808542813f00ef47058c20b0-300x300.png)
Lecture_9_2005
... Monitoring protein levels - large scale • 2D gel electrophoresis – Old technology - not as useful for lowly expressed proteins. ...
... Monitoring protein levels - large scale • 2D gel electrophoresis – Old technology - not as useful for lowly expressed proteins. ...
chapter 5 large biological molecules
... o Tertiary structure – 3-D irregular structure that results from bonding between side chains of the various amino acids; Types of bonding: hydrophobic interaction, Van der Waals forces, H bonds, ionic bonds, and disulfide bridges. o Quaternary structure – if it has 2 or more polypeptide chains. De ...
... o Tertiary structure – 3-D irregular structure that results from bonding between side chains of the various amino acids; Types of bonding: hydrophobic interaction, Van der Waals forces, H bonds, ionic bonds, and disulfide bridges. o Quaternary structure – if it has 2 or more polypeptide chains. De ...
PowerPoint
... the hydrodynamic properties of proteins so that you understand why things work the way they do ...
... the hydrodynamic properties of proteins so that you understand why things work the way they do ...
Organic Molecule
... Label your model and write the chemical formula beneath the model. Animals store energy reserves as lipids. Plants store energy reserves as starches. What is the advantage of storing energy in lipids? Build a saturated fatty acid model Build an unsaturated fatty acid model ...
... Label your model and write the chemical formula beneath the model. Animals store energy reserves as lipids. Plants store energy reserves as starches. What is the advantage of storing energy in lipids? Build a saturated fatty acid model Build an unsaturated fatty acid model ...
Chapter 2 Chemical Basis of Life
... Proteins: Proteins have a great variety of functions in the body—as structural materials, as energy sources, as certain hormones, as receptors on cell membranes, as antibodies, and as enzymes to catalyze metabolic reactions. Proteins contain what four elements? Building blocks of proteins are the am ...
... Proteins: Proteins have a great variety of functions in the body—as structural materials, as energy sources, as certain hormones, as receptors on cell membranes, as antibodies, and as enzymes to catalyze metabolic reactions. Proteins contain what four elements? Building blocks of proteins are the am ...
Slide 1
... The E. coli member of this family, GroEL forms a double-layered cylindrical structure which provides a protected environment in which proteins can fold. The structure is capped at one end by another heat-shock protein: GroES, a member of the Hsp 10 family. The Hsp 90 family • All are named because t ...
... The E. coli member of this family, GroEL forms a double-layered cylindrical structure which provides a protected environment in which proteins can fold. The structure is capped at one end by another heat-shock protein: GroES, a member of the Hsp 10 family. The Hsp 90 family • All are named because t ...
BIOCHEMISTRY - Mexico Central School District
... • Many Nucleotides make up DNA! DNA = genetic instructions that direct a cell's structure and function. The ribosomes are instructed (by DNA) on which types of protein a cell will make DNA is found in the chromosomes of the nucleus (and a few other organelles) ...
... • Many Nucleotides make up DNA! DNA = genetic instructions that direct a cell's structure and function. The ribosomes are instructed (by DNA) on which types of protein a cell will make DNA is found in the chromosomes of the nucleus (and a few other organelles) ...
Plant Cells and Tissues
... Cuticular wax: embedded in the cuticle, primary layer of wax, 2 types Cutin: makes up most of cuticle Suberin: underground plant parts Waxes: more water repellent & harder than other lipids ...
... Cuticular wax: embedded in the cuticle, primary layer of wax, 2 types Cutin: makes up most of cuticle Suberin: underground plant parts Waxes: more water repellent & harder than other lipids ...
File
... Proteins: Proteins have a great variety of functions in the body---as structural materials, as energy sources, as certain hormones, as receptors on cell membranes, as antibodies, and as enzymes to catalyze metabolic reactions. Proteins contain what 4 elements? Building blocks of proteins are the ami ...
... Proteins: Proteins have a great variety of functions in the body---as structural materials, as energy sources, as certain hormones, as receptors on cell membranes, as antibodies, and as enzymes to catalyze metabolic reactions. Proteins contain what 4 elements? Building blocks of proteins are the ami ...
Chapter 2 Study Outline
... joined together; complex carbohydrates called _____________, such as starch, are built of many sugars. Humans synthesize the complex carbohydrate called _______________. Lipids: Three kinds: What 3 elements do they all contain? _________________ supply energy, are built from glycerol and three fatty ...
... joined together; complex carbohydrates called _____________, such as starch, are built of many sugars. Humans synthesize the complex carbohydrate called _______________. Lipids: Three kinds: What 3 elements do they all contain? _________________ supply energy, are built from glycerol and three fatty ...
Protein Structure and Folding
... 1. Use SCOP (Structural Classification Of Proteins) http://scop.mrc-lmb.cam.ac.uk/scop/ to classify PDB entry 1tml. 2. Name the fold of central domain of 1m6h and draw the corresponding topology diagram. 3. Classify the two domains of a metabolic regulator protein 1d66 from Baker’s yeast. 4. Use DAL ...
... 1. Use SCOP (Structural Classification Of Proteins) http://scop.mrc-lmb.cam.ac.uk/scop/ to classify PDB entry 1tml. 2. Name the fold of central domain of 1m6h and draw the corresponding topology diagram. 3. Classify the two domains of a metabolic regulator protein 1d66 from Baker’s yeast. 4. Use DAL ...
Document
... Observe aspect of nature & ask a question. Develop a hypothesis (tentative answer). Using hypothesis, make a prediction. Test prediction with experiments, observations, models, etc; evaluate & analyze results. Objectively report results & conclusions. Scientific theory: An accepted body ...
... Observe aspect of nature & ask a question. Develop a hypothesis (tentative answer). Using hypothesis, make a prediction. Test prediction with experiments, observations, models, etc; evaluate & analyze results. Objectively report results & conclusions. Scientific theory: An accepted body ...
Polyacrylamide gels
... Polyacrylamide gel electrophoresis (PAGE) Electrophoresis in a polyacrylamide matrix separating or resolving molecules in a mixture under the influence of an applied electric field •PAGE used for proteins and small pieces of DNA •Similar idea to separation of DNA in agarose •2 major determinants of ...
... Polyacrylamide gel electrophoresis (PAGE) Electrophoresis in a polyacrylamide matrix separating or resolving molecules in a mixture under the influence of an applied electric field •PAGE used for proteins and small pieces of DNA •Similar idea to separation of DNA in agarose •2 major determinants of ...
Lecture 3
... one polypeptide chain or multimeric having more than one chain. In case of a homomultimer the chains are of one kind whereas for a heteromultimer two or more different chains form the protein. (e.g. Hemoglobin is a heterotetramer. It has two alpha chains and two beta chains.) Proteins may be simple ...
... one polypeptide chain or multimeric having more than one chain. In case of a homomultimer the chains are of one kind whereas for a heteromultimer two or more different chains form the protein. (e.g. Hemoglobin is a heterotetramer. It has two alpha chains and two beta chains.) Proteins may be simple ...
Section 2.3 Review Sheet
... A polymer is a large molecule made up of smaller units, called monomers, which are linked together. One analogy could be a string of Christmas lights – each light is a monomer and linked together they form a polymer. 2. What elements make up a carbohydrate? Carbon, hydrogen and oxygen (each glucose ...
... A polymer is a large molecule made up of smaller units, called monomers, which are linked together. One analogy could be a string of Christmas lights – each light is a monomer and linked together they form a polymer. 2. What elements make up a carbohydrate? Carbon, hydrogen and oxygen (each glucose ...
Prezentace aplikace PowerPoint
... *transfer of separated proteins from the gel onto a membrane *identification of a protein by a complex of primary and secondary antibodies *visualization by color reaction or by ...
... *transfer of separated proteins from the gel onto a membrane *identification of a protein by a complex of primary and secondary antibodies *visualization by color reaction or by ...
1415 DNA Tic Tac Toe TEAM 1-1
... 1. What is the name of a portion of DNA that codes for a specific protein? (a gene) 2. What is transcription? (the process in which a portion of DNA is copied and made into an mRNA strand) 3. What has to happen with DNA before a cell can divide? (DNA replication) 4. Enzymes are what kind of BioMolec ...
... 1. What is the name of a portion of DNA that codes for a specific protein? (a gene) 2. What is transcription? (the process in which a portion of DNA is copied and made into an mRNA strand) 3. What has to happen with DNA before a cell can divide? (DNA replication) 4. Enzymes are what kind of BioMolec ...
Absolute quantification of proteins and phosphoproteins from cell
... 2-dimensional gel electrophoresis tandem MS ...
... 2-dimensional gel electrophoresis tandem MS ...
BIOGRAPHICAL SKETCH Abhijeet Kapoor Postdoctoral Research
... transitions in Ras proteins with the goal to identify specific structural features controlling the intrinsic conformational transitions, and complement the results using all-atom simulations. First, I developed the coarse-grained model that successfully folded nineteen proteins to their native state ...
... transitions in Ras proteins with the goal to identify specific structural features controlling the intrinsic conformational transitions, and complement the results using all-atom simulations. First, I developed the coarse-grained model that successfully folded nineteen proteins to their native state ...
Bio200 Au13 Lec19 10-29 Slides
... • Post-translational modifications can be used by the cell to regulate ...
... • Post-translational modifications can be used by the cell to regulate ...
Diversity of Cell Envelops in the Archaea.
... layer or S-layer surrounding the cell membrane in contrast to the peptidoglycan-containing envelopes of the gram positive and gram negative bacteria. The archaeal S-layers are typically assembled from one or two abundant and often post-translationally modified proteins that are exported from the cel ...
... layer or S-layer surrounding the cell membrane in contrast to the peptidoglycan-containing envelopes of the gram positive and gram negative bacteria. The archaeal S-layers are typically assembled from one or two abundant and often post-translationally modified proteins that are exported from the cel ...
Cyclol
![](https://commons.wikimedia.org/wiki/Special:FilePath/Cyclol_reaction.png?width=300)
The cyclol hypothesis is the first structural model of a folded, globular protein. It was developed by Dorothy Wrinch in the late 1930s, and was based on three assumptions. Firstly, the hypothesis assumes that two peptide groups can be crosslinked by a cyclol reaction (Figure 1); these crosslinks are covalent analogs of non-covalent hydrogen bonds between peptide groups. These reactions have been observed in the ergopeptides and other compounds. Secondly, it assumes that, under some conditions, amino acids will naturally make the maximum possible number of cyclol crosslinks, resulting in cyclol molecules (Figure 2) and cyclol fabrics (Figure 3). These cyclol molecules and fabrics have never been observed. Finally, the hypothesis assumes that globular proteins have a tertiary structure corresponding to Platonic solids and semiregular polyhedra formed of cyclol fabrics with no free edges. Such ""closed cyclol"" molecules have not been observed either.Although later data demonstrated that this original model for the structure of globular proteins needed to be amended, several elements of the cyclol model were verified, such as the cyclol reaction itself and the hypothesis that hydrophobic interactions are chiefly responsible for protein folding. The cyclol hypothesis stimulated many scientists to research questions in protein structure and chemistry, and was a precursor of the more accurate models hypothesized for the DNA double helix and protein secondary structure. The proposal and testing of the cyclol model also provides an excellent illustration of empirical falsifiability acting as part of the scientific method.