MCAD - MSOE Center for BioMolecular Modeling
... Intermolecular Interactions Modeled in MCAD, FAD and ETF MCAD Active Site: Glu376, Glu199, Leu103, Ser142, Met249, Asp253, Arg388 Folding of MCAD: 2 Dimers Combine: Arg28:A and Glu86:D Tetramer: Lys304, Glu300, Gln342, Asp346, Arg383 ...
... Intermolecular Interactions Modeled in MCAD, FAD and ETF MCAD Active Site: Glu376, Glu199, Leu103, Ser142, Met249, Asp253, Arg388 Folding of MCAD: 2 Dimers Combine: Arg28:A and Glu86:D Tetramer: Lys304, Glu300, Gln342, Asp346, Arg383 ...
Species Absorbing in the 500-nm Region in the Reactions of
... Metal ion mediated nonenzymatic reactions of pyridoxal (PL) and amino acid derivatives and those of pyridoxamine (PM) and a -keto acid derivatives have been proved to be useful tools for the mechanistic investigation of PL functions.3) We found that in methanol solutions PM and ester of an a keto ac ...
... Metal ion mediated nonenzymatic reactions of pyridoxal (PL) and amino acid derivatives and those of pyridoxamine (PM) and a -keto acid derivatives have been proved to be useful tools for the mechanistic investigation of PL functions.3) We found that in methanol solutions PM and ester of an a keto ac ...
Designing logical codon reassignment
... organisms and even animals.10 Other systems based on the reassignment of the ochre and opal stop codons,11 in addition to the use of quadruplet codons have also been reported (see later section).12 It is now also possible to consider their combined use for the incorporation of multiple distinct UAAs ...
... organisms and even animals.10 Other systems based on the reassignment of the ochre and opal stop codons,11 in addition to the use of quadruplet codons have also been reported (see later section).12 It is now also possible to consider their combined use for the incorporation of multiple distinct UAAs ...
Synthetic biology for engineering acetyl coenzyme a
... The study also clearly demonstrated that functional PDH activity in the cytosol required the addition of lipoic acid to the medium. The cytosolic localization of the enzyme was demonstrated by subcellular fractionation followed by gel filtration and mass spectrometry. Furthermore, enzyme activity co ...
... The study also clearly demonstrated that functional PDH activity in the cytosol required the addition of lipoic acid to the medium. The cytosolic localization of the enzyme was demonstrated by subcellular fractionation followed by gel filtration and mass spectrometry. Furthermore, enzyme activity co ...
- BioMedSearch
... effective anti-influenza agent (see [16]). E. coli, overexpressing PEPS, are widely used in the biosynthesis of shikimic acid as is over-expression of 3-deoxy-D-arabino-heptulosonic acid 7-phosphate (DAHP) synthase whose use can be limited by feedback inhibition. Overexpression of feedback insensiti ...
... effective anti-influenza agent (see [16]). E. coli, overexpressing PEPS, are widely used in the biosynthesis of shikimic acid as is over-expression of 3-deoxy-D-arabino-heptulosonic acid 7-phosphate (DAHP) synthase whose use can be limited by feedback inhibition. Overexpression of feedback insensiti ...
Enzyme Mechanisms - Weber State University
... Enzyme Mechanisms – Chymotrypsin, Trypsin, Elastase ...
... Enzyme Mechanisms – Chymotrypsin, Trypsin, Elastase ...
Active site mapping, biochemical properties and
... designed to interact with the S2, S1 and S% subsites of the active site cleft revealed differences between rhodesain and the related trypanosome protease cruzain. Using fluorogenic dipeptidyl substrates, rhodesain and cruzain had acid pH optima, but unlike some mammalian cathepsins retained signific ...
... designed to interact with the S2, S1 and S% subsites of the active site cleft revealed differences between rhodesain and the related trypanosome protease cruzain. Using fluorogenic dipeptidyl substrates, rhodesain and cruzain had acid pH optima, but unlike some mammalian cathepsins retained signific ...
Chapter 1 - Research Explorer
... (BCAA), was one of the first organic acidemias to be described, almost 50 years ago by Tanaka et al. (1966). Subsequently, Budd et al. (1967) clarified the primary clinical manifestations of the disease. A large number of case studies have progressively emphasized the clinical heterogeneity of IVA ( ...
... (BCAA), was one of the first organic acidemias to be described, almost 50 years ago by Tanaka et al. (1966). Subsequently, Budd et al. (1967) clarified the primary clinical manifestations of the disease. A large number of case studies have progressively emphasized the clinical heterogeneity of IVA ( ...
glycogen metabolism
... • glycogen synthase, is the key regulatory enzyme in glycogen synthesis. • It can add glucosyl residues only if the polysaccharide chain already contains more than four residues. • Thus, glycogen synthesis requires a primer. – This priming function is carried out by glycogenin, a protein composed o ...
... • glycogen synthase, is the key regulatory enzyme in glycogen synthesis. • It can add glucosyl residues only if the polysaccharide chain already contains more than four residues. • Thus, glycogen synthesis requires a primer. – This priming function is carried out by glycogenin, a protein composed o ...
Combined fluorescence and electrochemical investigation on the
... is a heart-shaped molecule composed of a single-chain of 585 amino acid residues with a molecular weight of 66400 Da. As first reported by Carter’s group (Carter and Ho, 1994; He and Carter, 1992), HSA is made of three homologous domains (I, II and III), each with two subdomains (A and B). The main s ...
... is a heart-shaped molecule composed of a single-chain of 585 amino acid residues with a molecular weight of 66400 Da. As first reported by Carter’s group (Carter and Ho, 1994; He and Carter, 1992), HSA is made of three homologous domains (I, II and III), each with two subdomains (A and B). The main s ...
BIOCHEMISTRY Carbohydrate Metabolism
... • Step 3 – Phosphofructokinase (an allosteric enzyme) is inhibited by high levels of ATP & Citrate & is activated by high levels of ADP. • Step 10 – Pyruvate kinase (an allosteric enzyme) is inhibited by high levels of ATP & Acetyl CoA. ...
... • Step 3 – Phosphofructokinase (an allosteric enzyme) is inhibited by high levels of ATP & Citrate & is activated by high levels of ADP. • Step 10 – Pyruvate kinase (an allosteric enzyme) is inhibited by high levels of ATP & Acetyl CoA. ...
Characteristics of Sophorolipid as an Antimicrobial Agent
... biosurfactant that was produced from Pseudomonas sp., showed antimicrobial, antifungal, mycoplasmicidal, and antiviral activities [24, 45]. Surfactin and iturin, lipopeptidetype biosurfactants, inhibited bacterial cell growth by disintegrating the cellular membrane [1, 3] and showed some antiviral a ...
... biosurfactant that was produced from Pseudomonas sp., showed antimicrobial, antifungal, mycoplasmicidal, and antiviral activities [24, 45]. Surfactin and iturin, lipopeptidetype biosurfactants, inhibited bacterial cell growth by disintegrating the cellular membrane [1, 3] and showed some antiviral a ...
citric acid metabolism in lactic bacteria and
... The timing of post-fermentation sulphiting also has a major impact. Close monitoring of the L-malic acid degradation kinetics during MLF (assays on a twice-weekly basis) is recommended when diacetyl content is a major concern for the winemaker. This enables the winemaker to sulphite the wine rapidly ...
... The timing of post-fermentation sulphiting also has a major impact. Close monitoring of the L-malic acid degradation kinetics during MLF (assays on a twice-weekly basis) is recommended when diacetyl content is a major concern for the winemaker. This enables the winemaker to sulphite the wine rapidly ...
chap18 oxidative phosphorylation
... Oxidative pphosphorylation produces 30 of the 32 molecules of ATP that are formed when glucose is oxidized to CO2 and H2O. The process is conceptually easy but mechanistically very difficult. The electron flow from NADH and FADH2 to oxygen through protein complexes leads to pumping of protons outsid ...
... Oxidative pphosphorylation produces 30 of the 32 molecules of ATP that are formed when glucose is oxidized to CO2 and H2O. The process is conceptually easy but mechanistically very difficult. The electron flow from NADH and FADH2 to oxygen through protein complexes leads to pumping of protons outsid ...
Physiology of the thermophilic acetogen Moorella - The Keep
... it cannot be excluded that M. thermoacetica translocates sodium ions. Indeed, under certain conditions, sodium-‐proton antiporters may be involved in the formation of electrochemical gradients by M. thermoacetica ...
... it cannot be excluded that M. thermoacetica translocates sodium ions. Indeed, under certain conditions, sodium-‐proton antiporters may be involved in the formation of electrochemical gradients by M. thermoacetica ...
Russell, M.J. and Hall, A.J. 2006.
... convection in the oceanic crust, was resolved by the onset of life. We suggest that this chemosynthetic life emerged within hydrothermal mounds produced by alkaline solutions of moderate temperature in the relative safety of the deep ocean floor. Exothermic reaction between hydrothermal H2, HCOO - a ...
... convection in the oceanic crust, was resolved by the onset of life. We suggest that this chemosynthetic life emerged within hydrothermal mounds produced by alkaline solutions of moderate temperature in the relative safety of the deep ocean floor. Exothermic reaction between hydrothermal H2, HCOO - a ...
Associative Nitrogen-fixation Bacteria and Cyanobacteria.
... The discovery, in Arabidopsis, of two cytochrome P450 enzymes (CYP79B2 and CYT79B3) that catalyze the formation of IAox from Trp, suggests that at least a portion of IAN could be Trp-derived via an IAox intermediate. IAN may also be regarded as a degradation product resulting from the turnover of in ...
... The discovery, in Arabidopsis, of two cytochrome P450 enzymes (CYP79B2 and CYT79B3) that catalyze the formation of IAox from Trp, suggests that at least a portion of IAN could be Trp-derived via an IAox intermediate. IAN may also be regarded as a degradation product resulting from the turnover of in ...
Key Residues Controlling Binding of Diverse Ligands to Human
... CYP2A enzymes. In several cases, other P450 proteins are known to adapt to different ligands in a more induced-fit mode of binding, frustrating attempts to predict drug binding and metabolism. If the relatively small CYP2A selectivity is based on a more steric-dominated, lock-andkey type model, then ...
... CYP2A enzymes. In several cases, other P450 proteins are known to adapt to different ligands in a more induced-fit mode of binding, frustrating attempts to predict drug binding and metabolism. If the relatively small CYP2A selectivity is based on a more steric-dominated, lock-andkey type model, then ...
Computational Studies of Multi-Active Site Enzymes
... relative free energies of the rate-limiting barriers as well as that of the hydrolyzed products for Thr-AMP (14-19 and 4-10 kcal mol-1, respectively) are markedly higher than for Ser-AMP (7-12 and 0-5 kcal mol-1, respectively). That is, MST1 thermodynamically and kinetically preferentially edits aga ...
... relative free energies of the rate-limiting barriers as well as that of the hydrolyzed products for Thr-AMP (14-19 and 4-10 kcal mol-1, respectively) are markedly higher than for Ser-AMP (7-12 and 0-5 kcal mol-1, respectively). That is, MST1 thermodynamically and kinetically preferentially edits aga ...
Acids and Bases
... aspirin and vitamin C are acetylsalicylic acid and ascorbic acid; both will produce H+ ions when dissolved in water. Acetic acid (HC2 H3 O2 ) is the primary component in vinegar, and formic acid (HCO2 H) is what causes ant bites to sting. Hydrochloric acid (HCl) is stomach acid, phosphoric acid (H3 ...
... aspirin and vitamin C are acetylsalicylic acid and ascorbic acid; both will produce H+ ions when dissolved in water. Acetic acid (HC2 H3 O2 ) is the primary component in vinegar, and formic acid (HCO2 H) is what causes ant bites to sting. Hydrochloric acid (HCl) is stomach acid, phosphoric acid (H3 ...