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Role of the non-respiratory pathways in the utilization of molecular
Role of the non-respiratory pathways in the utilization of molecular

... Saccharomyces cerevisiae is a facultative anaerobe devoid of mitochondrial alternative oxidase. In this yeast, the structure and biogenesis of the respiratory chain, on the one hand, and the functional interactions of oxidative phosphorylation with the cellular energetic metabolism, on the other, ar ...
The Proposed Effects of Nicotinamide Adenine Dinucleotide (NAD
The Proposed Effects of Nicotinamide Adenine Dinucleotide (NAD

... metabolism. In the same study it was found that in insect flight muscles the activities of both citrate synthase and NAD+ linked isocitrate dehydrogenase are high, indicating that the muscles involved in flight for insects depend on aerobic metabolism for energy production. The study concerns insect ...
Characterization of the cDNA and Gene Coding for the Biotin
Characterization of the cDNA and Gene Coding for the Biotin

... has severa1 unusual features, including imperfect palindromes and polypyrimidine sequences, which may function in the transcriptional regulation of the 9\02 gene. ...
Modelling malic acid accumulation in fruits: relationships
Modelling malic acid accumulation in fruits: relationships

... accumulated essentially in the vacuoles of mesocarp cells that contain >85–90% of the total malic acid content (Yamaki, 1984). Malic acid can be synthesized in the fruit itself or supplied to the fruit by the phloem and xylem saps. However, the pH of the saps is above 5–6 in the xylem and above 7 in ...
43803_C000 1..20
43803_C000 1..20

... Printed in the United States of America on acid-free paper ...
Influence of Aerobic and Phototrophic Growth
Influence of Aerobic and Phototrophic Growth

Influence of Aerobic and Phototrophic Growth
Influence of Aerobic and Phototrophic Growth

... the reaction 6-PG was converted to pyruvate and glyceraldehyde 3-phosphate by the action of 6-PG dehydratase and KDPG aldolase, which were also present in the extract, and that the apparent NAD-dependent 6-PG dehydrogenase activity was due to glyceraldehyde3-phosphate dehydrogenase ; the same side r ...
Catalytic mechanism of nucleoside diphosphate kinase investigated
Catalytic mechanism of nucleoside diphosphate kinase investigated

... kinase for most studies since it is the NDP kinase best characterized by crystallography (7, 12, 14), physical studies (25, 31), mutagenesis (32), and fast kinetic studies (15, 33). Kinetic parameters for TDP and several analogues are given in Table 2. All compounds lacking the 3′-OH group are poor ...
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... • Recemase acts upon D-methyl malonyl CoA to give L-methyl malonyl CoA. • This reaction is essential for the entry of this compound into metabolic reactions of body. ...
Scientific Opinion on Dietary Reference Values for protein
Scientific Opinion on Dietary Reference Values for protein

Open the publication - UEF Electronic Publications
Open the publication - UEF Electronic Publications

... diabetes, certain cancers and mental problems, such as depression and schizophrenia16,19. Pgc1α, key regulator of cellular metabolism, has been identified as the key link between changes in metabolism and circadian clock. At the molecular level, the circadian clock represents a complex gene regulato ...
Intermediary metabolism
Intermediary metabolism

Stressrelated challenges in pentose fermentation to ethanol
Stressrelated challenges in pentose fermentation to ethanol

... be used in the SSF to stay within the allowable temperature for the yeast. An improved yeast thermotolerance would thus be beneficial from a process point of view. Also the end-product of the fermentation, ethanol, is in itself inhibitory at high levels (see [15] for a recent review), but in its pre ...
The demonstration of nickel in the urease of Helicobacter pylori by
The demonstration of nickel in the urease of Helicobacter pylori by

... present only in the 61 kDa polypeptide and in the ratio of between five and six atoms to one molecule of urease, suggesting a hexameric structure. These results are discussed in relation to other bacterial ureases and urease activity at low pH. ...
informed bodybuilding nutrition
informed bodybuilding nutrition

... However, we may name product examples, and if only one product of that type exists on the market, then it may be cited. We may also discuss the pros and cons of competitive products, so consumers are able to make an informed choice for themselves. I will attempt to cover all aspects of nutrition rel ...
13-Alanine as an Ethylene Precursor
13-Alanine as an Ethylene Precursor

... cotyledons to metabolize malonate could exert control over the biosynthesis of ethylene in this tissue. Acetyl CoA is produced by plant tissue in the metabolism of both propionate (8) and malonate (7). The similarity in tihe respiration profiles of these 2 substances certainly suggests that their me ...
Science
Science

... mechanism of SIRT1 activation (4, 15). To elucidate the determinants of activation in SIRT1, we screened for SIRT1 mutant proteins lacking activation (Fig. 2C). The ability of SIRT1 to be activated by resveratrol was attenuated in one mutant that substituted a lysine for a glutamate at position 230 ...
Isolation and Fractionation 2
Isolation and Fractionation 2

... Although this technique separates mitochondria from lysosomes, both fractions tend to be contaminated by peroxisomes as judged by the distribution of catalase activity. Selective Modification of the Density of Lysosomes The size and density of these organelles can be selectively modified by 'indige ...
Kinetic analysis of retroviral proteases by HELGA EIZERT
Kinetic analysis of retroviral proteases by HELGA EIZERT

... antagonist for the host cells’ innate defense system, by neutralization of cellular APOBEC3 protein functions. Bel-3 does not have a defined role in the viral life cycle. ...
University of Groningen Lactococcus lactis systems biology Eckhardt
University of Groningen Lactococcus lactis systems biology Eckhardt

... down into smaller peptides and amino acids by a large number of peptidases. Peptidases with different specificities have been described 27,28. The obtained knowledge was essential for the engineering of flavor formation in the production of cheese 29,30. The most important proteins in proteolysis ar ...
Eukaryote-Like Serine/Threonine Kinases and Phosphatases in
Eukaryote-Like Serine/Threonine Kinases and Phosphatases in

... families composed of enzymes that show similar substrate specificities and modes of regulation (63). This review focuses on bacterial kinases with catalytic domains that share structural and functional homology with eukaryotic Ser/Thr kinases. These kinases are referred to herein as eSTKs to disting ...
Application of Synthetic Biology for Biopolymer
Application of Synthetic Biology for Biopolymer

... PHB from lignocellulosic biomass may be reduced since both glucose and xylose fractions of the substrate can be used. Although the generated yeast strains were able to consume xylose and produce PHB, the production remained low. To tackle this problem, other enzymes from different ix ...
Fibrinogen Bern I: Substitution y 337 Asn + Lys Is
Fibrinogen Bern I: Substitution y 337 Asn + Lys Is

... Bern I, was detected in a clinically asymptomatic woman. Thrombin and reptilase clotting times were prolonged. Fibrinogen concentration was decreased when measured by the functional assay but was normal by the heat precipitation method (Table 1). Family study confirmed the hereditary nature of this ...
Instructions for use Title ENZYMOLOGICAL STUDIES ON
Instructions for use Title ENZYMOLOGICAL STUDIES ON

Plasma and Vascular Tissue Arginine Are Decreased in Diabetes
Plasma and Vascular Tissue Arginine Are Decreased in Diabetes

... concentrations of the endothelium-dependent vasodilator, ACH. We have previously shown that relaxation to ACH is virtually blocked in both control and diabetic rat aorta by NO synthase inhibitors (Pieper and Peltier, 1995; Pieper et al., 1995). For these studies, the rings were challenged once with ...
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Amino acid synthesis

Amino acid synthesis is the set of biochemical processes (metabolic pathways) by which the various amino acids are produced from other compounds. The substrates for these processes are various compounds in the organism's diet or growth media. Not all organisms are able to synthesise all amino acids. Humans are excellent example of this, since humans can only synthesise 11 of the 20 standard amino acids (aka non-essential amino acid), and in time of accelerated growth, arginine, can be considered an essential amino acid.A fundamental problem for biological systems is to obtain nitrogen in an easily usable form. This problem is solved by certain microorganisms capable of reducing the inert N≡N molecule (nitrogen gas) to two molecules of ammonia in one of the most remarkable reactions in biochemistry. Ammonia is the source of nitrogen for all the amino acids. The carbon backbones come from the glycolytic pathway, the pentose phosphate pathway, or the citric acid cycle.In amino acid production, one encounters an important problem in biosynthesis, namely stereochemical control. Because all amino acids except glycine are chiral, biosynthetic pathways must generate the correct isomer with high fidelity. In each of the 19 pathways for the generation of chiral amino acids, the stereochemistry at the α-carbon atom is established by a transamination reaction that involves pyridoxal phosphate. Almost all the transaminases that catalyze these reactions descend from a common ancestor, illustrating once again that effective solutions to biochemical problems are retained throughout evolution.Biosynthetic pathways are often highly regulated such that building-blocks are synthesized only when supplies are low. Very often, a high concentration of the final product of a pathway inhibits the activity of enzymes that function early in the pathway. Often present are allosteric enzymes capable of sensing and responding to concentrations of regulatory species. These enzymes are similar in functional properties to aspartate transcarbamoylase and its regulators. Feedback and allosteric mechanisms ensure that all twenty amino acids are maintained in sufficient amounts for protein synthesis and other processes.
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