Use of the non-radioactive SUnSET method to detect decreased
... the effects of MG132—a potent proteasome inhibitor— on protein synthesis in root tissue. Initial experiments were aimed at establishing evidence that 50 μM MG132 inhibited the proteasome in the roots of Arabidopsis plants. As anticipated, proteasome inhibition caused by MG132 treatment increased lev ...
... the effects of MG132—a potent proteasome inhibitor— on protein synthesis in root tissue. Initial experiments were aimed at establishing evidence that 50 μM MG132 inhibited the proteasome in the roots of Arabidopsis plants. As anticipated, proteasome inhibition caused by MG132 treatment increased lev ...
The alphabet soup of plant intracellular signalling: enter cyclic
... physiological status of the cell, it allows signal damping. In nonhigher-plant eucaryotic systems, pathways of intracellular signalling are well established. Although plant biologists often draw parallels with animal systems, our knowledge of secondary signalling molecules in plants is fragmentary, ...
... physiological status of the cell, it allows signal damping. In nonhigher-plant eucaryotic systems, pathways of intracellular signalling are well established. Although plant biologists often draw parallels with animal systems, our knowledge of secondary signalling molecules in plants is fragmentary, ...
Defective HIV-1 Proviruses Can Be Transcribed Upon Activation
... Methods. To understand how T cell activation affects the transcription of HIV-1 proviruses, resting CD4+ T cells from aviremic patients under suppressive antiretroviral therapy were activated with anti-CD3/CD28 costimulation under enfuvirtide to prevent new rounds of in vitro infection. To examine w ...
... Methods. To understand how T cell activation affects the transcription of HIV-1 proviruses, resting CD4+ T cells from aviremic patients under suppressive antiretroviral therapy were activated with anti-CD3/CD28 costimulation under enfuvirtide to prevent new rounds of in vitro infection. To examine w ...
Clathrinmediated transport: assembly required
... using cryo-electron microscopy and 3D-image processing techniques. The transition from a non-constricted to a constricted state was accompanied by a change from a straight to a zigzag pattern for the GTPase effector domain/middle region of dynamin. Hinshaw suggested that this conformational change r ...
... using cryo-electron microscopy and 3D-image processing techniques. The transition from a non-constricted to a constricted state was accompanied by a change from a straight to a zigzag pattern for the GTPase effector domain/middle region of dynamin. Hinshaw suggested that this conformational change r ...
lect6
... Know that the product from pur ine degradation is uric acid and why it can cause medical problems . Explain so me of the bene fit s to medicine of unde rstand ing nitrogen metaboli sm. ...
... Know that the product from pur ine degradation is uric acid and why it can cause medical problems . Explain so me of the bene fit s to medicine of unde rstand ing nitrogen metaboli sm. ...
Strategies for Attaching Oligonucleotides to Solid Supports
... and other enzymes. Polystyrene magnetic beads are available with both carboxylic acid and amine surface chemistries. Silica micro-spheres have a relatively homogeneous chemical surface that can easily be modified using versatile and well developed silanization chemistry [1]. Their use in molecular ...
... and other enzymes. Polystyrene magnetic beads are available with both carboxylic acid and amine surface chemistries. Silica micro-spheres have a relatively homogeneous chemical surface that can easily be modified using versatile and well developed silanization chemistry [1]. Their use in molecular ...
The goal of protein structure prediction by threading is to find a best
... structures generally are similar as well. Thus, in naturally occurring proteins, sequences that are similar to the query sequence carry useful information about its 3D structure. A multiple sequence alignment centered on the query sequence reflects sequence variability within the protein family to w ...
... structures generally are similar as well. Thus, in naturally occurring proteins, sequences that are similar to the query sequence carry useful information about its 3D structure. A multiple sequence alignment centered on the query sequence reflects sequence variability within the protein family to w ...
MagNA Lyser Instrument
... Aliquot containing 10 mg sample material (here mouse and human research samples) was taken to purify RNA either with the MagNA Pure LC RNA Isolation Kit III (Tissue) or the MagNA Pure LC mRNA Isolation Kit II (Tissue) homogenized with the MagNA Lyser Instrument. ** Yield and purity strongly depend o ...
... Aliquot containing 10 mg sample material (here mouse and human research samples) was taken to purify RNA either with the MagNA Pure LC RNA Isolation Kit III (Tissue) or the MagNA Pure LC mRNA Isolation Kit II (Tissue) homogenized with the MagNA Lyser Instrument. ** Yield and purity strongly depend o ...
How Long Does it Take to Manufacture Plasmid under GMP?
... How Long Does it Take to Manufacture Plasmid under GMP? Manufacturing plasmid under GMP generally takes four to nine months, depending upon your project’s specific requirements. You may be wondering, “Why does it take so long to produce a plasmid under GMP when making a plasmid in a lab only takes a ...
... How Long Does it Take to Manufacture Plasmid under GMP? Manufacturing plasmid under GMP generally takes four to nine months, depending upon your project’s specific requirements. You may be wondering, “Why does it take so long to produce a plasmid under GMP when making a plasmid in a lab only takes a ...
Bacteria - Ector County ISD
... - some damage the cell walls or prevent new cell wall from forming - some damage the cell membrane - some prevent protein synthesis - some prevent DNA from being copied - some interfere with bacterial metabolism ...
... - some damage the cell walls or prevent new cell wall from forming - some damage the cell membrane - some prevent protein synthesis - some prevent DNA from being copied - some interfere with bacterial metabolism ...
Information Content in Genetics:
... reverse transcription from RNA to DNA and then back to RNA. ...
... reverse transcription from RNA to DNA and then back to RNA. ...
Chromatography Resins for Protein Purification
... purification of biomolecules. Pall offers a line of chromatography resins ideal for protein purification applications (see Table 4.6). This broad line of chromatography products exhibits superior performance and is useful for affinity, ion exchange, size exclusion, and hydrophobic interaction chroma ...
... purification of biomolecules. Pall offers a line of chromatography resins ideal for protein purification applications (see Table 4.6). This broad line of chromatography products exhibits superior performance and is useful for affinity, ion exchange, size exclusion, and hydrophobic interaction chroma ...
Proteins As Drugs - The Major Products of the Biotechnology Industry
... the rat gene neu, and is therefore sometimes referred to as HER2/neu or cerbB-2. • Many normal cells express a small amount of HER2 protein on their plasma membranes in a tissue-specific pattern. • The HER2 receptor has no known ligand; however, HER2 forms heterodimers with HER1 (the epidermal growt ...
... the rat gene neu, and is therefore sometimes referred to as HER2/neu or cerbB-2. • Many normal cells express a small amount of HER2 protein on their plasma membranes in a tissue-specific pattern. • The HER2 receptor has no known ligand; however, HER2 forms heterodimers with HER1 (the epidermal growt ...
Isolation of a Transforming Sequence from a Human Bladder
... DNA (Shih et al., 1979, 1981; Cooper et al., 1980; Krontiris and Cooper, 1981; Perucho et al., 1981). Since normal cellular DNAs, studied in parallel, seemed to lack such competence, it was concluded that the actively oncogenic sequences of tumor cell DNA arose during the process of carcinogenesis, ...
... DNA (Shih et al., 1979, 1981; Cooper et al., 1980; Krontiris and Cooper, 1981; Perucho et al., 1981). Since normal cellular DNAs, studied in parallel, seemed to lack such competence, it was concluded that the actively oncogenic sequences of tumor cell DNA arose during the process of carcinogenesis, ...
PDF version of paper
... 3. Export the finished product as a commonly used image format file. 4. Include this in your document. XFig and Inkscape could be employed for doing these with high efficiency and it produces figures that are neater. Figures that take hours in presentation software could be easily prepared using XFi ...
... 3. Export the finished product as a commonly used image format file. 4. Include this in your document. XFig and Inkscape could be employed for doing these with high efficiency and it produces figures that are neater. Figures that take hours in presentation software could be easily prepared using XFi ...
Mark scheme
... if not all lines used, go back and credit further correct points DO NOT ACCEPT growth of cells DO NOT ACCEPT repair of cells ...
... if not all lines used, go back and credit further correct points DO NOT ACCEPT growth of cells DO NOT ACCEPT repair of cells ...
Biology Accelerated v. 2016
... substance is produced, maintained, and destroyed, and by which energy is made available; Nucleic Acids- any of a group of long, linear macromolecules, either DNA or various types of RNA, that carry ...
... substance is produced, maintained, and destroyed, and by which energy is made available; Nucleic Acids- any of a group of long, linear macromolecules, either DNA or various types of RNA, that carry ...
On The Determination of Enzyme Structure, Function, and
... Enzymes are linear polymers of similar building blocks called amino acids (see Figure 1). Amino acids are either obtained from food or synthesized in cells, and polymerized according to the instructions of the genes of the organism. Enzyme molecules fold into three-dimensional structures in order to ...
... Enzymes are linear polymers of similar building blocks called amino acids (see Figure 1). Amino acids are either obtained from food or synthesized in cells, and polymerized according to the instructions of the genes of the organism. Enzyme molecules fold into three-dimensional structures in order to ...
SI and non-SI units for Biological quantities
... responses remains embedded in the approach to controlling this type of medicine -A reductionist approach to measurement in this field, breaking down the system into measurable components, seems unpromising – biological systems are not the sum of their parts -Developing a metrology for complex system ...
... responses remains embedded in the approach to controlling this type of medicine -A reductionist approach to measurement in this field, breaking down the system into measurable components, seems unpromising – biological systems are not the sum of their parts -Developing a metrology for complex system ...
Porphyrin
... Biosynthesis. Porphyrins and their related macrocycles found in nature are derived by the same biosynthetic pathway (Fig. 3). Glycine and succinic acid are condensed to yield 8-aminolevulinic acid, which is dimerized to the pyrrole porphobilinogen. Four units of porphobilinogen are coupled to give u ...
... Biosynthesis. Porphyrins and their related macrocycles found in nature are derived by the same biosynthetic pathway (Fig. 3). Glycine and succinic acid are condensed to yield 8-aminolevulinic acid, which is dimerized to the pyrrole porphobilinogen. Four units of porphobilinogen are coupled to give u ...
rna interference
... RNA INTERFERENCE SLIDES 1, 2 The natural functions of RNA interference are as follows: (1) antiviral mechanism, (2) regulation of gene expression (?). There is not too much data on the regulatory role of RNA interference in vivo. RNA interference (RNAi) is a mechanism in molecular biology where the ...
... RNA INTERFERENCE SLIDES 1, 2 The natural functions of RNA interference are as follows: (1) antiviral mechanism, (2) regulation of gene expression (?). There is not too much data on the regulatory role of RNA interference in vivo. RNA interference (RNAi) is a mechanism in molecular biology where the ...
Eukaryotic DNA Replication
... Any segment of DNA that has an origin should be able to replicate, so although plasmids are rare in eukaryotes, it may be possible to construct them by suitable manipulation in vivo. This has been accomplished in yeast, although not in higher eukaryotes. The discovery of yeast origins resulted ...
... Any segment of DNA that has an origin should be able to replicate, so although plasmids are rare in eukaryotes, it may be possible to construct them by suitable manipulation in vivo. This has been accomplished in yeast, although not in higher eukaryotes. The discovery of yeast origins resulted ...
Case study from Almac
... •14C present in the skeleton of all drug molecules. • 14C is Detectable at very low concentrations (scintillation counting) • Long half life means no need for correction for radioactive decay. •3H is also used but is more subject to exchange. ...
... •14C present in the skeleton of all drug molecules. • 14C is Detectable at very low concentrations (scintillation counting) • Long half life means no need for correction for radioactive decay. •3H is also used but is more subject to exchange. ...
Cell-penetrating peptide
Cell-penetrating peptides (CPPs) are short peptides that facilitate cellular uptake of various molecular cargo (from nanosize particles to small chemical molecules and large fragments of DNA). The ""cargo"" is associated with the peptides either through chemical linkage via covalent bonds or through non-covalent interactions. The function of the CPPs are to deliver the cargo into cells, a process that commonly occurs through endocytosis with the cargo delivered to the endosomes of living mammalian cells.CPPs hold great potential as in vitro and in vivo delivery vectors for use in research and medicine. Current use is limited by a lack of cell specificity in CPP-mediated cargo delivery and insufficient understanding of the modes of their uptake.CPPs typically have an amino acid composition that either contains a high relative abundance of positively charged amino acids such as lysine or arginine or has sequences that contain an alternating pattern of polar/charged amino acids and non-polar, hydrophobic amino acids. These two types of structures are referred to as polycationic or amphipathic, respectively. A third class of CPPs are the hydrophobic peptides, containing only apolar residues, with low net chargeor have hydrophobic amino acid groups that are crucial for cellular uptake.The first CPP was discovered independently by two laboratories in 1988, when it was found that the trans-activating transcriptional activator (TAT) from human immunodeficiency virus 1 (HIV-1) could be efficiently taken up from the surrounding media by numerous cell types in culture. Since then, the number of known CPPs has expanded considerably and small molecule synthetic analogues with more effective protein transduction properties have been generated.