PEGylation Technique and scope of it`s Applications
... increased half-life, decreased plasma clearance, and a shift in distribution in favour of diseased tissues. PEG is incorporated into the lipid bilayer of the liposome, forming a hydrated shell that protects it from destruction by proteins. For the antitumour drug doxorubicin, peglyation of the lip ...
... increased half-life, decreased plasma clearance, and a shift in distribution in favour of diseased tissues. PEG is incorporated into the lipid bilayer of the liposome, forming a hydrated shell that protects it from destruction by proteins. For the antitumour drug doxorubicin, peglyation of the lip ...
Biology Class IX for SA-I 2016-17
... The cell membrane is selectively permeable and regulates themovement of substances in and out of the cell. Movement of CO2: CO2 is produced during cellular respiration. Therefore, it is present in high concentrations inside the cell. This CO2 must be excreted out of the cell. In the cell’s external ...
... The cell membrane is selectively permeable and regulates themovement of substances in and out of the cell. Movement of CO2: CO2 is produced during cellular respiration. Therefore, it is present in high concentrations inside the cell. This CO2 must be excreted out of the cell. In the cell’s external ...
MID 4 Rachel J. Gordon, MD, MPH Introduction to Antimicrobials
... 2) Inhibition of protein synthesis 3) Alteration nucleic acid metabolism 4) Inhibition of folate metabolism--necessary for production of DNA and other essential metabolites. 5) Miscellaneous mechanisms include disruption of the cell membrane and production of free radicals that damage DNA. An ‘antib ...
... 2) Inhibition of protein synthesis 3) Alteration nucleic acid metabolism 4) Inhibition of folate metabolism--necessary for production of DNA and other essential metabolites. 5) Miscellaneous mechanisms include disruption of the cell membrane and production of free radicals that damage DNA. An ‘antib ...
At the Intersection of the Pathways for Exocytosis and
... subsequently being identified (Lipschutz & Mostov, 2002). In yeast, this complex consists of six secretion related proteins (Sec), Sec3, Sec5, Sec6, Sec8, Sec10 and Sec15, with an additional two subunits known as exocyst related proteins (Exo), Exo70 and Exo84. Tethering to the plasma membrane is me ...
... subsequently being identified (Lipschutz & Mostov, 2002). In yeast, this complex consists of six secretion related proteins (Sec), Sec3, Sec5, Sec6, Sec8, Sec10 and Sec15, with an additional two subunits known as exocyst related proteins (Exo), Exo70 and Exo84. Tethering to the plasma membrane is me ...
Biology+Term+List
... cardiovascular system The human circulatory system consisting of the heart and the vessels cast Type of fossil preservation where the original material of the fossil has decayed and been replaced later by another material, much the way a plaster cast is made in a mold. catabolic reactions Reactions ...
... cardiovascular system The human circulatory system consisting of the heart and the vessels cast Type of fossil preservation where the original material of the fossil has decayed and been replaced later by another material, much the way a plaster cast is made in a mold. catabolic reactions Reactions ...
DNA and RNA:
... 7.1 The Central Dogma Proteins play a critical role in how cells successfully meet the challenges of living. Cells use proteins to maintain their shape and to speed up important chemical reactions such as photosynthesis and respiration. A cell will not live long if it cannot reliably create the prot ...
... 7.1 The Central Dogma Proteins play a critical role in how cells successfully meet the challenges of living. Cells use proteins to maintain their shape and to speed up important chemical reactions such as photosynthesis and respiration. A cell will not live long if it cannot reliably create the prot ...
Section 7.1 DNA Cloning with Plasmid Vectors
... Plasmids are circular, double-stranded DNA (dsDNA) molecules that are separate from a cell’s chromosomal DNA. These extrachromosomal DNAs, which occur naturally in bacteria, yeast, and some higher eukaryotic cells, exist in a parasitic or symbiotic relationship with their host cell. Plasmids range i ...
... Plasmids are circular, double-stranded DNA (dsDNA) molecules that are separate from a cell’s chromosomal DNA. These extrachromosomal DNAs, which occur naturally in bacteria, yeast, and some higher eukaryotic cells, exist in a parasitic or symbiotic relationship with their host cell. Plasmids range i ...
intact-protein based sample preparation strategies for
... needs for high sensitivity and high throughput. They are relied upon for protein quantification, identification, and analysis of post-translational modifications (PTMs). The standard technique of two dimensional poly-acrylamide gel electrophoresis (2D PAGE) offers relatively limited resolution and s ...
... needs for high sensitivity and high throughput. They are relied upon for protein quantification, identification, and analysis of post-translational modifications (PTMs). The standard technique of two dimensional poly-acrylamide gel electrophoresis (2D PAGE) offers relatively limited resolution and s ...
open - The Student Room
... The marking schemes which follow were those used by WJEC for the January 2013 examination in GCE BIOLOGY/HUMAN BIOLOGY. They were finalised after detailed discussion at examiners' conferences by all the examiners involved in the assessment. The conferences were held shortly after the papers were tak ...
... The marking schemes which follow were those used by WJEC for the January 2013 examination in GCE BIOLOGY/HUMAN BIOLOGY. They were finalised after detailed discussion at examiners' conferences by all the examiners involved in the assessment. The conferences were held shortly after the papers were tak ...
High Resolution Structure of the Neisseria gonorrhoeae Type IV
... crystal structures of Type IV pilins from several important human pathogens using the macromolecular crystallography beamlines at SSRL (Beamlines 7-1, 9-1, 9-2 and 11-1). This success built directly upon the earlier efforts of Tainer group members Hans Parge and Andrew Arvai who made the original br ...
... crystal structures of Type IV pilins from several important human pathogens using the macromolecular crystallography beamlines at SSRL (Beamlines 7-1, 9-1, 9-2 and 11-1). This success built directly upon the earlier efforts of Tainer group members Hans Parge and Andrew Arvai who made the original br ...
molecular physiology
... b) Electrical charge – This membrane is negatively charged due to presence of glycoproteins rich in silica acid. Therefore the negatively charged molecules are less permeable than neutral and positive charged molecules. This is the reason that albumin, although has 7nm molecular diameter, is not fil ...
... b) Electrical charge – This membrane is negatively charged due to presence of glycoproteins rich in silica acid. Therefore the negatively charged molecules are less permeable than neutral and positive charged molecules. This is the reason that albumin, although has 7nm molecular diameter, is not fil ...
Nucleic Acid and Protein Quantitation Methods
... Invitrogen kits but is a known mutagen so precautions must be taken when using the dye. The Invitrogen Kits have lower detection limits but Hoechst has a wider detection range; again making the choice of dye specific to the application. In addition to kits for dsDNA, Invitrogen has developed reagent ...
... Invitrogen kits but is a known mutagen so precautions must be taken when using the dye. The Invitrogen Kits have lower detection limits but Hoechst has a wider detection range; again making the choice of dye specific to the application. In addition to kits for dsDNA, Invitrogen has developed reagent ...
Gene Section PAWR (PRKC apoptosis WT1 regulator protein) in Oncology and Haematology
... rat Par-4) which is 100% conserved in human, mouse and rat. Apoptosis by ectopic Par-4/PAWR requires Par-4/PAWR nuclear translocation and involves both activation of the Fas death receptor signaling pathway and NF-kappaB inhibition. Par-4/PAWR also inhibits the prosurvival protein Bcl-2 and down reg ...
... rat Par-4) which is 100% conserved in human, mouse and rat. Apoptosis by ectopic Par-4/PAWR requires Par-4/PAWR nuclear translocation and involves both activation of the Fas death receptor signaling pathway and NF-kappaB inhibition. Par-4/PAWR also inhibits the prosurvival protein Bcl-2 and down reg ...
Chemistry of Life
... – structure • peptide bonds join amino acids together – covalent » forms by dehydration reaction btwn amino and carboxyl groups on a pair of amino acids – has partial double bond characteristic – is stiff » amino acids are not free to rotate around the C-N linkage » makes it possible for chains of a ...
... – structure • peptide bonds join amino acids together – covalent » forms by dehydration reaction btwn amino and carboxyl groups on a pair of amino acids – has partial double bond characteristic – is stiff » amino acids are not free to rotate around the C-N linkage » makes it possible for chains of a ...
Product Manual - GenTarget Inc
... including stem cells, primary cells, and non-dividing cells both in vivo and in vitro. Lentiviral Particles stably integrate into the transduced cells’ genome for long term expression, making it a great gene transfer agent. Myoglobin (MB) Promoter: Myoglobin is an iron- and oxygen-binding protein fo ...
... including stem cells, primary cells, and non-dividing cells both in vivo and in vitro. Lentiviral Particles stably integrate into the transduced cells’ genome for long term expression, making it a great gene transfer agent. Myoglobin (MB) Promoter: Myoglobin is an iron- and oxygen-binding protein fo ...
39 | the respiratory system
... of thin-walled parenchymal cells, typically one-cell thick, that look like tiny bubbles within the sacs. Alveoli are in direct contact with capillaries (one-cell thick) of the circulatory system. Such intimate contact ensures that oxygen will diffuse from alveoli into the blood and be distributed to ...
... of thin-walled parenchymal cells, typically one-cell thick, that look like tiny bubbles within the sacs. Alveoli are in direct contact with capillaries (one-cell thick) of the circulatory system. Such intimate contact ensures that oxygen will diffuse from alveoli into the blood and be distributed to ...
Green Fluorescent Protein (GFP) Purification Kit
... activity with the bacteria they genetically transformed using the plasmid, pGLO. Transformed bacteria which produce the genetically engineered Green Fluorescent Protein (GFP) are removed from their agar plates and allowed to multiply in liquid nutrient media. The bacterial cells are then broken open ...
... activity with the bacteria they genetically transformed using the plasmid, pGLO. Transformed bacteria which produce the genetically engineered Green Fluorescent Protein (GFP) are removed from their agar plates and allowed to multiply in liquid nutrient media. The bacterial cells are then broken open ...
Cell Biology
... Living things Like you, the atmosphere is also made mostly of carbon, hydrogen, have complex and oxygen. But the atmosphere is not alive. The key to life is how molecules these elements are put together. In the atmosphere, they are in the form of simple compounds like carbon dioxide and water. In li ...
... Living things Like you, the atmosphere is also made mostly of carbon, hydrogen, have complex and oxygen. But the atmosphere is not alive. The key to life is how molecules these elements are put together. In the atmosphere, they are in the form of simple compounds like carbon dioxide and water. In li ...
Cell Biology # 4
... • DNA helices separated into replication bubbles with replication forks at each end – Each strand acts as template for complementary strand ...
... • DNA helices separated into replication bubbles with replication forks at each end – Each strand acts as template for complementary strand ...
Chapter 3 PowerPoint - Hillsborough Community College
... • RNA polymerase adds complementary nucleotides to growing mRNA matching sequence of based on DNA template strand – Short, 12-base-pair segment where DNA and mRNA are temporarily bonded is referred to as DNA-RNA ...
... • RNA polymerase adds complementary nucleotides to growing mRNA matching sequence of based on DNA template strand – Short, 12-base-pair segment where DNA and mRNA are temporarily bonded is referred to as DNA-RNA ...
In vitro biological activities of Douglas fir essential oil in
... Abstract: Although essential oils from Douglas fir are popular topical skincare products, research regarding their biological effects on human skin cells is scarce. Here, we studied the biological activity of a commercially available Douglas fir (Pseudotsuga menziesii) essential oil (DEO) in a human ...
... Abstract: Although essential oils from Douglas fir are popular topical skincare products, research regarding their biological effects on human skin cells is scarce. Here, we studied the biological activity of a commercially available Douglas fir (Pseudotsuga menziesii) essential oil (DEO) in a human ...
Introductory Mycology BI 432/532 Lecture 2: Overview of
... • formerly part of Zygomycota (Glomales) ...
... • formerly part of Zygomycota (Glomales) ...
Luciferase Reporter Vector Protocol
... binding activity in vivo through the use of the standard luciferase assay. Each Luciferase Reporter Vector contains multiple repeats of a specific transcription factor binding element. Binding at this recognition site by the corresponding transcription factor results in the expression of luciferase, ...
... binding activity in vivo through the use of the standard luciferase assay. Each Luciferase Reporter Vector contains multiple repeats of a specific transcription factor binding element. Binding at this recognition site by the corresponding transcription factor results in the expression of luciferase, ...
Cell-penetrating peptide
Cell-penetrating peptides (CPPs) are short peptides that facilitate cellular uptake of various molecular cargo (from nanosize particles to small chemical molecules and large fragments of DNA). The ""cargo"" is associated with the peptides either through chemical linkage via covalent bonds or through non-covalent interactions. The function of the CPPs are to deliver the cargo into cells, a process that commonly occurs through endocytosis with the cargo delivered to the endosomes of living mammalian cells.CPPs hold great potential as in vitro and in vivo delivery vectors for use in research and medicine. Current use is limited by a lack of cell specificity in CPP-mediated cargo delivery and insufficient understanding of the modes of their uptake.CPPs typically have an amino acid composition that either contains a high relative abundance of positively charged amino acids such as lysine or arginine or has sequences that contain an alternating pattern of polar/charged amino acids and non-polar, hydrophobic amino acids. These two types of structures are referred to as polycationic or amphipathic, respectively. A third class of CPPs are the hydrophobic peptides, containing only apolar residues, with low net chargeor have hydrophobic amino acid groups that are crucial for cellular uptake.The first CPP was discovered independently by two laboratories in 1988, when it was found that the trans-activating transcriptional activator (TAT) from human immunodeficiency virus 1 (HIV-1) could be efficiently taken up from the surrounding media by numerous cell types in culture. Since then, the number of known CPPs has expanded considerably and small molecule synthetic analogues with more effective protein transduction properties have been generated.