Luciferase Reporter Vector Protocol
... binding activity in vivo through the use of the standard luciferase assay. Each Luciferase Reporter Vector contains multiple repeats of a specific transcription factor binding element. Binding at this recognition site by the corresponding transcription factor results in the expression of luciferase, ...
... binding activity in vivo through the use of the standard luciferase assay. Each Luciferase Reporter Vector contains multiple repeats of a specific transcription factor binding element. Binding at this recognition site by the corresponding transcription factor results in the expression of luciferase, ...
Looking at long molecules in solution: what happens when they are
... morphology as well as processes such as cell division. It is not a rigid permanent structure, it is dynamic and constantly rearranges by polymerising and depolymerising to produce movement. There are three major types of protein fibre within the cytoskeleton of eukaryotic cells: actin filaments; inte ...
... morphology as well as processes such as cell division. It is not a rigid permanent structure, it is dynamic and constantly rearranges by polymerising and depolymerising to produce movement. There are three major types of protein fibre within the cytoskeleton of eukaryotic cells: actin filaments; inte ...
Press Release
... decay started after translation was complete and that decaying RNA molecules provided little biological information. Now a team from EMBL Heidelberg and Stanford University led by Lars Steinmetz has turned this on its head. The researchers have shown that one end of the mRNA begins to decay while th ...
... decay started after translation was complete and that decaying RNA molecules provided little biological information. Now a team from EMBL Heidelberg and Stanford University led by Lars Steinmetz has turned this on its head. The researchers have shown that one end of the mRNA begins to decay while th ...
UNIT I
... sharing electrons. In fact, atoms give up little by sharing. For example, 2 Hydrogen atoms share their electron to have two electrons in their shell. Oxygen (1s2, 2s2, 2p4) shares two electrons to form O2. Methane is another example. Carbon has six electrons: 1s2, 2s2, 2p2. The carbon shares its fou ...
... sharing electrons. In fact, atoms give up little by sharing. For example, 2 Hydrogen atoms share their electron to have two electrons in their shell. Oxygen (1s2, 2s2, 2p4) shares two electrons to form O2. Methane is another example. Carbon has six electrons: 1s2, 2s2, 2p2. The carbon shares its fou ...
ribosome
... at a time. Click on the amino acid that will be 1st delivered to the ribosome? Hint: Examine the Genetic Code chart on your handout to find the 1st codon. Now that methionine is delivered, tRNA drops off methionine and the ribosome moves to the next codon. ...
... at a time. Click on the amino acid that will be 1st delivered to the ribosome? Hint: Examine the Genetic Code chart on your handout to find the 1st codon. Now that methionine is delivered, tRNA drops off methionine and the ribosome moves to the next codon. ...
BCH364C-391L_SyntheticBio1_Spring2015
... the outcome when we change the system - For molecular biology, this means: - designing new gene circuits and networks - modeling the designed systems & predicting their properties - making & testing the designs - updating our understanding from the model/test agreement Edward Marcotte/Univ. of Texas ...
... the outcome when we change the system - For molecular biology, this means: - designing new gene circuits and networks - modeling the designed systems & predicting their properties - making & testing the designs - updating our understanding from the model/test agreement Edward Marcotte/Univ. of Texas ...
ref. #27 of the TIBS article
... non-glycosylated; acceptor site) are introduced in different positions relative to the H1 and H2 transmembrane segments, as well as to a segment (H3) inserted into the P2 domain by site-directed mutagenesis. Proteins are expressed in vitro in the presence of dog pancreas microsomes, and the degree o ...
... non-glycosylated; acceptor site) are introduced in different positions relative to the H1 and H2 transmembrane segments, as well as to a segment (H3) inserted into the P2 domain by site-directed mutagenesis. Proteins are expressed in vitro in the presence of dog pancreas microsomes, and the degree o ...
Protein Tyrosine Nitration
... MacMillan-Crow LA, Crow JP, Kirby JD, Beckman JS, Thompson JA. (1996) Nitration and inactivation of manganese superoxide dismutase in chronic rejection of human renal allografts. Proc Natl Acad Sci USA. 93:11853-8. Giasson BI, Duda JE, Murray I, Chen Q, Souza JM, Hurting HI, Ischiropoulos H, Trojano ...
... MacMillan-Crow LA, Crow JP, Kirby JD, Beckman JS, Thompson JA. (1996) Nitration and inactivation of manganese superoxide dismutase in chronic rejection of human renal allografts. Proc Natl Acad Sci USA. 93:11853-8. Giasson BI, Duda JE, Murray I, Chen Q, Souza JM, Hurting HI, Ischiropoulos H, Trojano ...
Document
... The time window that can be addressed by simple 2D IR spectroscopy is limited by the lifetime of the vibrational transition, which is short with a few 10s of picoseconds in the most favorable case. While proteins are also dynamical on these fast timescales, biological relevant processes are typical ...
... The time window that can be addressed by simple 2D IR spectroscopy is limited by the lifetime of the vibrational transition, which is short with a few 10s of picoseconds in the most favorable case. While proteins are also dynamical on these fast timescales, biological relevant processes are typical ...
Polymers - Yafi Zayyat
... Condensation polymerization is the formation of successive links between small units called monomer molecules to form a long chained macromolecule ( polymer ) and a small molecule ( usually water). For example: Dicarboxylic acid + Diamines ...
... Condensation polymerization is the formation of successive links between small units called monomer molecules to form a long chained macromolecule ( polymer ) and a small molecule ( usually water). For example: Dicarboxylic acid + Diamines ...
Book Review - Journal of Experimental Biology
... of specific areas of metabolism, written by recognized experts in each of these fields. The first seven chapters provide an overview of the fundamental concepts of metabolism, along with molecular and biophysical descriptions of basic metabolic mechanisms. Chapter 1 deals with basic principles and a ...
... of specific areas of metabolism, written by recognized experts in each of these fields. The first seven chapters provide an overview of the fundamental concepts of metabolism, along with molecular and biophysical descriptions of basic metabolic mechanisms. Chapter 1 deals with basic principles and a ...
2.01 structure of cells.
... a. Those that contain their own DNA and genes. Mitochondria and plastids are organelles that reproduce by dividing like independent cells. b. Those that do not contain their own DNA; For example, endoplasmic reticulum, ribosomes, golgi bodies, undulipodia. ...
... a. Those that contain their own DNA and genes. Mitochondria and plastids are organelles that reproduce by dividing like independent cells. b. Those that do not contain their own DNA; For example, endoplasmic reticulum, ribosomes, golgi bodies, undulipodia. ...
biochem ch 45 [9-2
... Blood Plasma Proteins, Coagulation, and Fibrinolysis Osmotic pressure exerted by plasma proteins regulates distribution of water between blood and tissues Plasma proteins in junction with platelets maintain integrity of circulatory system through clotting Primary hemostatic plug consists of ag ...
... Blood Plasma Proteins, Coagulation, and Fibrinolysis Osmotic pressure exerted by plasma proteins regulates distribution of water between blood and tissues Plasma proteins in junction with platelets maintain integrity of circulatory system through clotting Primary hemostatic plug consists of ag ...
A Few Good Domains
... The RING finger is a specialized type of Zn finger consisting of 40–60 residues that binds two atoms of zinc, and is involved in mediating protein—protein interactions. The presence of a RING finger domain is a characteristic of RING-class E3 ubiquitin protein ligases capable of transfering ubiquiti ...
... The RING finger is a specialized type of Zn finger consisting of 40–60 residues that binds two atoms of zinc, and is involved in mediating protein—protein interactions. The presence of a RING finger domain is a characteristic of RING-class E3 ubiquitin protein ligases capable of transfering ubiquiti ...
Computational Biophysics
... 2.2 A Virtual screens for molecules that modulate collagen stability Triple-helical collagen is the most abundant protein in all mammals. It is hypothesized that naturally, collagen samples partially unfolded states. These states are accessed when one strand partially unwinds from the triple-helix. ...
... 2.2 A Virtual screens for molecules that modulate collagen stability Triple-helical collagen is the most abundant protein in all mammals. It is hypothesized that naturally, collagen samples partially unfolded states. These states are accessed when one strand partially unwinds from the triple-helix. ...
Assessing the biocompatibility of click
... phosphodiester linkage in Escherichia coli has been evaluated. The requirement for selective pressure on the click-containing gene was probed via a plasmid containing click DNA backbone linkages in each strand of the gene encoding the fluorescent protein mCherry. The effect of proximity of the click ...
... phosphodiester linkage in Escherichia coli has been evaluated. The requirement for selective pressure on the click-containing gene was probed via a plasmid containing click DNA backbone linkages in each strand of the gene encoding the fluorescent protein mCherry. The effect of proximity of the click ...
NCBI Protein Structure
... protein examples. Website can be easily accessed at the AP Bio Moodle site http://ab.thinkingdistance.org/login/index.php (Login with your username or as a guest). ...
... protein examples. Website can be easily accessed at the AP Bio Moodle site http://ab.thinkingdistance.org/login/index.php (Login with your username or as a guest). ...
Oligomerization and activation of the FliI ATPase
... FliI monomers assembling to the active oligomer, most likely a hexamer, at the cytosolic membrane to provide the energy for assembly at the critical location. Whether this energy is used directly for substrate protein translocation and/or protein unfolding or interaction is not known. Crystal struct ...
... FliI monomers assembling to the active oligomer, most likely a hexamer, at the cytosolic membrane to provide the energy for assembly at the critical location. Whether this energy is used directly for substrate protein translocation and/or protein unfolding or interaction is not known. Crystal struct ...
Automatic De Novo Sequencing of peptides by Electron Transfer
... display a characteristic sequence-independent fragmentation. Product ions have similar abundances except for those corresponding to different charge states of the intact precursor (Figure 2). Hence, ETD spectra carry little information codified on the ion abundances. In contrast, CID product ions ha ...
... display a characteristic sequence-independent fragmentation. Product ions have similar abundances except for those corresponding to different charge states of the intact precursor (Figure 2). Hence, ETD spectra carry little information codified on the ion abundances. In contrast, CID product ions ha ...
Quick Guide - Fulvic Acid
... Fulvic Acid metal organic complexes are relatively heavy and because of this, they have small molecular size and can easily penetrate cells. Fulvic Acid complexes and chelates are readily able to pass through semi-permeable membranes such as cell walls. Yet it is important to note that Fulvic Acid n ...
... Fulvic Acid metal organic complexes are relatively heavy and because of this, they have small molecular size and can easily penetrate cells. Fulvic Acid complexes and chelates are readily able to pass through semi-permeable membranes such as cell walls. Yet it is important to note that Fulvic Acid n ...
production and quality control of medicinal products derived
... natural sources can now be produced using such recombinant DNA (rDNA) technology. In addition, the ability to synthesise and manipulate nucleic acids allows the construction of genes coding for modified products possessing different properties from their natural counterpart, or even entirely novel p ...
... natural sources can now be produced using such recombinant DNA (rDNA) technology. In addition, the ability to synthesise and manipulate nucleic acids allows the construction of genes coding for modified products possessing different properties from their natural counterpart, or even entirely novel p ...
1. Introduction - diss.fu
... A diverse group of transposable elements relies solely on DNA intermediates without an RNA phase. These are called DNA transposons and they vary in size, structure and complexity, from small, simple insertion sequences (ISs) to more complex composite transposable elements (Mahillon and Chandler, 199 ...
... A diverse group of transposable elements relies solely on DNA intermediates without an RNA phase. These are called DNA transposons and they vary in size, structure and complexity, from small, simple insertion sequences (ISs) to more complex composite transposable elements (Mahillon and Chandler, 199 ...
Site directed mutagenesis as an efficient way to enhance structural
... Green fluorescence protein (GFP) was first found and isolated from a jelly fish Aequoreavictorea (Tsien, 1998).There are many coelenterates that have this protein but those that are well studied and characterised are from Aequorea and Renilla. But so far scientists were able to clone only GFP from A ...
... Green fluorescence protein (GFP) was first found and isolated from a jelly fish Aequoreavictorea (Tsien, 1998).There are many coelenterates that have this protein but those that are well studied and characterised are from Aequorea and Renilla. But so far scientists were able to clone only GFP from A ...
Cell Level Systems
... At Key Stage 4 students also need to understand DNA as a polymer made from four different nucleotides, each nucleotide consisting of a common sugar (deoxyribose) and phosphate group with one of four different bases (adenine, cytosine, guanine and thymine) attached to the sugar. The sequence of bases ...
... At Key Stage 4 students also need to understand DNA as a polymer made from four different nucleotides, each nucleotide consisting of a common sugar (deoxyribose) and phosphate group with one of four different bases (adenine, cytosine, guanine and thymine) attached to the sugar. The sequence of bases ...
Cell-penetrating peptide
Cell-penetrating peptides (CPPs) are short peptides that facilitate cellular uptake of various molecular cargo (from nanosize particles to small chemical molecules and large fragments of DNA). The ""cargo"" is associated with the peptides either through chemical linkage via covalent bonds or through non-covalent interactions. The function of the CPPs are to deliver the cargo into cells, a process that commonly occurs through endocytosis with the cargo delivered to the endosomes of living mammalian cells.CPPs hold great potential as in vitro and in vivo delivery vectors for use in research and medicine. Current use is limited by a lack of cell specificity in CPP-mediated cargo delivery and insufficient understanding of the modes of their uptake.CPPs typically have an amino acid composition that either contains a high relative abundance of positively charged amino acids such as lysine or arginine or has sequences that contain an alternating pattern of polar/charged amino acids and non-polar, hydrophobic amino acids. These two types of structures are referred to as polycationic or amphipathic, respectively. A third class of CPPs are the hydrophobic peptides, containing only apolar residues, with low net chargeor have hydrophobic amino acid groups that are crucial for cellular uptake.The first CPP was discovered independently by two laboratories in 1988, when it was found that the trans-activating transcriptional activator (TAT) from human immunodeficiency virus 1 (HIV-1) could be efficiently taken up from the surrounding media by numerous cell types in culture. Since then, the number of known CPPs has expanded considerably and small molecule synthetic analogues with more effective protein transduction properties have been generated.