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Protein Synthesis - No Brain Too Small
Protein Synthesis - No Brain Too Small

... The following questions were collated from the Level 3 AS 90715 since Protein Synthesis was examined there. Protein synthesis was not previously examined in a Level 2 AS. ...
downloadable  file
downloadable file

... sequence. Next, you add a DNA priming sequence, the four nucleotides and an enzyme called DNA polymerase which incorporates new nucleotide bases making a new piece of DNA which is a copy of the original piece. In Sanger’s original method, four different sequencing reactions are performed. Each react ...
Dr Asmat Salim MM 707 Molecular biology
Dr Asmat Salim MM 707 Molecular biology

... Most gene expression assays are based on the comparison of two or more samples and require uniform sampling conditions for comparison to be valid. ...
RNA & Protein Synthesis
RNA & Protein Synthesis

... ones break off leaving just amino acids bonded to each other  This continues until one of the three STOP codons is met  Finished amino acid strand goes through protein folding ...
Loading Complete Instructions: Choose the best answer for each
Loading Complete Instructions: Choose the best answer for each

... 28) All BUT one factor contributes to natural selection. That factor is A) mutation. B) random mating. C) genetic drift. D) environmental factors. 29) Scientists say that climate data from nine different countries indicate that every country will be hit with major climate changes during this century ...
Ch. 17 Protein Synthesis
Ch. 17 Protein Synthesis

Document
Document

... genetically identical cells produced from a single cell. • In 1997, Dolly the sheep was the first clone of an adult mammal. It took over 500 tries. ...
Dr. Becker`s Review – Exam 4 Notes provided by Kadie Keen
Dr. Becker`s Review – Exam 4 Notes provided by Kadie Keen

... 3. Apply 3’ tail (polyA – tail …AAAAAAAAA)  Mature mRNA goes to translation (ribosomes are attaching at the same time) NOTE: Proteins that are released from the cell into the body are processed by the ribosomes attached to the ER (rough ER). Proteins that will be used within the cell are processed ...
HOW ARE PROTEINS MADE?
HOW ARE PROTEINS MADE?

... A human’s genome is all the base pairs that compose the DNA of the organism, and their location on th chromosome. Human cells contain about 30,000 to 40,000 genes. ...
Class4 1-6 Win16 Enzymes and Nucleic Acids Notes
Class4 1-6 Win16 Enzymes and Nucleic Acids Notes

... it induces a shape change that makes the active site either: Left) more available to substrate, or Right) less available to substrate ...
Theory of PCR and its Applications
Theory of PCR and its Applications

... • It was discovered that a type of bacterial enzyme was found to have the ability to cut DNA in a test tube. • These restriction endonucleases, cut double stranded DNA at specific sites. • In a bacterial cell, restriction endonucleases (restriction enzymes) act as a kind of immune system, protecting ...
The elabration of RAMD-PCR assay for detection of a
The elabration of RAMD-PCR assay for detection of a

... A. Physical map of black gene showing introns (In 1-2) and exons (Ex 1-3). B. Sizes and location of the black gene fragments studied with forward (F) and reverse (R) primers ...
DNA- The Molecule of Life
DNA- The Molecule of Life

...  Composition: DNA is composed of nucleotides. The nucleotide is the monomer. ...
App1PCR - FSU Biology
App1PCR - FSU Biology

... as the human genome). Using PCR, picogram quantities of target DNA can be amplified to yield microgram quantities for subsequent biochemical analysis. In order to perform PCR, the DNA sequence flanking both sides of the target must be known. (Note that PCR cannot be used as a substitute for cloning ...
AS 90729 version 2 Describe genetic processes Level 3 Credits 4
AS 90729 version 2 Describe genetic processes Level 3 Credits 4

... PCR: Collect a small sample and amplify with PCR to produce an exact copy and a larger quantity of the DNA. (Note: PCR duplicates any DNA with accuracy and in large amounts. If contaminated with foreign DNA this would mean that any subsequent testing would produce results that are of little use.) Mo ...
DNA, RNA and Protein
DNA, RNA and Protein

... This process produces two exact DNA molecules (Chromosomes) that are the same DNA never leaves the nucleus ...
Mark scheme - biologypost
Mark scheme - biologypost

... Same (restriction) enzyme also cuts DNA; into which gene is inserted/plasmid/virus/Agrobacterium; (DNA) ligase; Joins two pieces of DNA together/forms recombinant DNA; Vector needed to insert DNA into host/plasmid enters host/second ...
PRACTICE EXAM ANSWERS 2007 1. A. Essentially
PRACTICE EXAM ANSWERS 2007 1. A. Essentially

... You should know that the Jak Stat pathway regulates this tumor suppressor, and that TS5 induction is good. IL25 is a cytokine that will cause the upregulation of TS5. IL25 binds to the receptor on the outside of the cell activating a signal transduction pathway that ultimately leads to expression of ...
to view and/or print October 2016 eDay assignment.
to view and/or print October 2016 eDay assignment.

... Read Your DNA makes you unique and explain how each human is unique or different if all humans have the same genes arranged in the same order. ...
More Exam Practice - Iowa State University
More Exam Practice - Iowa State University

... tRNA base-pairs with start codon AUG, and then the large ribosomal subunit binds b. Elongation- the mRNA is pulled through the ribosome so a new codon is exposed in the A site and a charged tRNA docks in the A site. The mRNA will be pulled through again, which will make the first bound tRNA in the P ...
Document
Document

... 9. What are the subatomic particles? Which ones are found in the nucleus of the atom? 10. How many electrons are found in each energy level (for the first 20 elements)? 11. Describe each type of bond: ionic, covalent and hydrogen. 12. Know the properties of water, including polarity, cohesion and ad ...
Support for Evolution
Support for Evolution

... Somatic cell mutations are not passed on to the next generation. Mutations that occur in sex cells are passed on to the organism’s offspring and will be present in every cell of the offspring. ...
DNA Basics - Haiku Learning : Login
DNA Basics - Haiku Learning : Login

... use of Rosalind Franklin’s X-ray diffraction photograph, W&C determined double helix structure ...
DNA, genes and chromosomes
DNA, genes and chromosomes

... causative agent of cholera, has two circular chromosomes.) The chromosome - together with ribosomes and proteins associated with gene expression - is located in a region of the cell cytoplasm known as the nucleoid. The genomes of prokaryotes are compact compared with those of eukaryotes, as they lac ...
Deamination of 5-methylcytosine yields thymine
Deamination of 5-methylcytosine yields thymine

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Deoxyribozyme



Deoxyribozymes, also called DNA enzymes, DNAzymes, or catalytic DNA, are DNA oligonucleotides that are capable of catalyzing specific chemical reactions, similar to the action of other biological enzymes, such as proteins or ribozymes (enzymes composed of RNA).However, in contrast to the abundance of protein enzymes in biological systems and the discovery of biological ribozymes in the 1980s,there are no known naturally occurring deoxyribozymes.Deoxyribozymes should not be confused with DNA aptamers which are oligonucleotides that selectively bind a target ligand, but do not catalyze a subsequent chemical reaction.With the exception of ribozymes, nucleic acid molecules within cells primarily serve as storage of genetic information due to its ability to form complementary base pairs, which allows for high-fidelity copying and transfer of genetic information. In contrast, nucleic acid molecules are more limited in their catalytic ability, in comparison to protein enzymes, to just three types of interactions: hydrogen bonding, pi stacking, and metal-ion coordination. This is due to the limited number of functional groups of the nucleic acid monomers: while proteins are built from up to twenty different amino acids with various functional groups, nucleic acids are built from just four chemically similar nucleobases. In addition, DNA lacks the 2'-hydroxyl group found in RNA which limits the catalytic competency of deoxyribozymes even in comparison to ribozymes.In addition to the inherent inferiority of DNA catalytic activity, the apparent lack of naturally occurring deoxyribozymes may also be due to the primarily double-stranded conformation of DNA in biological systems which would limit its physical flexibility and ability to form tertiary structures, and so would drastically limit the ability of double-stranded DNA to act as a catalyst; though there are a few known instances of biological single-stranded DNA such as multicopy single-stranded DNA (msDNA), certain viral genomes, and the replication fork formed during DNA replication. Further structural differences between DNA and RNA may also play a role in the lack of biological deoxyribozymes, such as the additional methyl group of the DNA base thymidine compared to the RNA base uracil or the tendency of DNA to adopt the B-form helix while RNA tends to adopt the A-form helix. However, it has also been shown that DNA can form structures that RNA cannot, which suggests that, though there are differences in structures that each can form, neither is inherently more or less catalytic due to their possible structural motifs.
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