Restriction Enzyme Digestion
... The sugar-phosphate backbone of one strand The sugar-phosphate backbone of both strands The nitrogenous bases from one strand The nitrogenous bases from both strands ...
... The sugar-phosphate backbone of one strand The sugar-phosphate backbone of both strands The nitrogenous bases from one strand The nitrogenous bases from both strands ...
Study Guide - first half of semester
... presented, you should know its 1) substrate preference (e.g., RNA or DNA, single strand or double strand), 2) what the products of the reaction are and 3) applications of the enzyme to molecular biological research. In addition to the enzyme list, we discussed general features of bacteria (genotype ...
... presented, you should know its 1) substrate preference (e.g., RNA or DNA, single strand or double strand), 2) what the products of the reaction are and 3) applications of the enzyme to molecular biological research. In addition to the enzyme list, we discussed general features of bacteria (genotype ...
File
... ________________________________________________________________________ o Hershey/Chase wanted to determined which part of the bacteriophage (the protein coat or nucleic acid core) entered the infected cell o They thought this would allow them to learn whether genes are made of protein or DNA o The ...
... ________________________________________________________________________ o Hershey/Chase wanted to determined which part of the bacteriophage (the protein coat or nucleic acid core) entered the infected cell o They thought this would allow them to learn whether genes are made of protein or DNA o The ...
ANTH 2301 - Week 4 DNA
... Parental strand of DNA unzips, exposing two daughter strands of DNA Free-floating RNA matches up to one exposed section of DNA ...
... Parental strand of DNA unzips, exposing two daughter strands of DNA Free-floating RNA matches up to one exposed section of DNA ...
Name
... l. What is a mutation? A change to the DNA. m. Distinguish between the following types of mutations: i. Silent – Does not affect protein synthesis – the mutation codes for the same amino acid. ii. Missense – A different amino acid is used during protein synthesis (a substitution). iii. Nonsense – A ...
... l. What is a mutation? A change to the DNA. m. Distinguish between the following types of mutations: i. Silent – Does not affect protein synthesis – the mutation codes for the same amino acid. ii. Missense – A different amino acid is used during protein synthesis (a substitution). iii. Nonsense – A ...
From Gene to Protein—Transcription and Translation
... 20. To summarize what you have learned about translation, explain how an mRNA molecule directs the synthesis of a protein. Include in your answer the words amino acid, anti-codon, codon, mRNA, protein, ribosome, tRNA, and translation. (you can use the back ) ...
... 20. To summarize what you have learned about translation, explain how an mRNA molecule directs the synthesis of a protein. Include in your answer the words amino acid, anti-codon, codon, mRNA, protein, ribosome, tRNA, and translation. (you can use the back ) ...
Bio 309F
... -27. A woman was found to have a mosaic disorder called anhidrotic ectodermal dysplasia where patches of skin have either sweat glands or no sweat glands. How would one account for this? A. X-inactivation B. autosomal recessive trait C. autosomal dominant trait D. A, B,and C could account for the m ...
... -27. A woman was found to have a mosaic disorder called anhidrotic ectodermal dysplasia where patches of skin have either sweat glands or no sweat glands. How would one account for this? A. X-inactivation B. autosomal recessive trait C. autosomal dominant trait D. A, B,and C could account for the m ...
Chapter 13 – Genetic Engineering
... • In animals – hybrids produced may be weaker and sterile – Ex – wolf x dog ---- weak wolf-dog – Ex – horse x donkey ---- mule (sterile) ...
... • In animals – hybrids produced may be weaker and sterile – Ex – wolf x dog ---- weak wolf-dog – Ex – horse x donkey ---- mule (sterile) ...
Biotechnology
... and primers attach (by H bonds) to their complementary sequences on either side of the target sequence 3. Elongation – the mixture is brought to 72 oC for several minutes during which polymerase binds and extends a DNA complement from each primer ...
... and primers attach (by H bonds) to their complementary sequences on either side of the target sequence 3. Elongation – the mixture is brought to 72 oC for several minutes during which polymerase binds and extends a DNA complement from each primer ...
Directed Reading A
... c. cells and structures b. generations d. protein and DNA ______ 2. What is the name of the material that determines inherited characteristics? a. deoxyribonucleic acid c. RNA b. ribosome d. amino acid ...
... c. cells and structures b. generations d. protein and DNA ______ 2. What is the name of the material that determines inherited characteristics? a. deoxyribonucleic acid c. RNA b. ribosome d. amino acid ...
FINAL EXAM PRACTICE TEST DNA The coded information in a
... B. A single antigen may stimulate the immune system to make many antibodies to it. C. Most antigens are proteins or large polysaccharides on the surfaces of viruses or foreign cells. D. Each antibody has only one antigen-binding site. 40.) Which of the following distinguishes the secondary immune re ...
... B. A single antigen may stimulate the immune system to make many antibodies to it. C. Most antigens are proteins or large polysaccharides on the surfaces of viruses or foreign cells. D. Each antibody has only one antigen-binding site. 40.) Which of the following distinguishes the secondary immune re ...
1 Molecular Genetics
... By this stage , It was NOT known whether the gene was simply a protein, or whether it was composed of DNA ...
... By this stage , It was NOT known whether the gene was simply a protein, or whether it was composed of DNA ...
Transcription/Translation foldable
... • WHAT? DNA is transcribed into mRNA. That means I am copying DNA and making mRNA. • Location? Nucleus • Why? DNA cannot leave the nucleus, so the messenger RNA has to take the nucleotide sequence to the ribosome to make proteins. Cut out the picture below. Label and color the DNA blue and the mRNA ...
... • WHAT? DNA is transcribed into mRNA. That means I am copying DNA and making mRNA. • Location? Nucleus • Why? DNA cannot leave the nucleus, so the messenger RNA has to take the nucleotide sequence to the ribosome to make proteins. Cut out the picture below. Label and color the DNA blue and the mRNA ...
Lab 8
... 4. Use the mRNA codon chart found below to associate the codons with particular amino acids. 5. Remember that tRNA molecules have anticodons, and carry amino acids to the ribosome. Identify the anticodon for each mRNA codon. 6. A bond forms between tyrosine (Tyr) and phenylalanine (Phe). This contri ...
... 4. Use the mRNA codon chart found below to associate the codons with particular amino acids. 5. Remember that tRNA molecules have anticodons, and carry amino acids to the ribosome. Identify the anticodon for each mRNA codon. 6. A bond forms between tyrosine (Tyr) and phenylalanine (Phe). This contri ...
PROTEIN SYNTHESIS
... catalyze chemical reactions, they make pigments, they form antigens and antibodies, give structure to cells and membranes, transport substances in cells and across membranes, and they perform hundreds of other functions. So, HOW ARE PROTEINS MADE? ...
... catalyze chemical reactions, they make pigments, they form antigens and antibodies, give structure to cells and membranes, transport substances in cells and across membranes, and they perform hundreds of other functions. So, HOW ARE PROTEINS MADE? ...
LipoJet DNA In Vitro Transfection Reagent
... Step 1: 1x105 cells are seeded in 24-well plate in 1 ml of appropriate growth medium containing serum and antibiotics on the day before transfection. Incubate the cells at 37 0C and 5 % CO2. The plate should be 60~80% confluent on the day of transfection. One hour before transfection, the serumconta ...
... Step 1: 1x105 cells are seeded in 24-well plate in 1 ml of appropriate growth medium containing serum and antibiotics on the day before transfection. Incubate the cells at 37 0C and 5 % CO2. The plate should be 60~80% confluent on the day of transfection. One hour before transfection, the serumconta ...
S1.Describe the important events that occur during the three stages
... S4. The process of transcriptional termination is not as well understood in eukaryotes as it is in bacteria. Nevertheless, current evidence suggests that there are several different mechanisms for termination. Like bacteria, the termination of certain genes appears to occur via intrinsic terminators ...
... S4. The process of transcriptional termination is not as well understood in eukaryotes as it is in bacteria. Nevertheless, current evidence suggests that there are several different mechanisms for termination. Like bacteria, the termination of certain genes appears to occur via intrinsic terminators ...
Genetics 3500 winter Test ii_ansers
... Multidrug resistance in cancer therapy results from the increased expression of specific membrane pumps that prevent the accumulation of a wide variety of chemotherapeutic agents in the cancer cells. Explain how the technique of real time PCR could be used to determine the levels of MDR-1 in a biops ...
... Multidrug resistance in cancer therapy results from the increased expression of specific membrane pumps that prevent the accumulation of a wide variety of chemotherapeutic agents in the cancer cells. Explain how the technique of real time PCR could be used to determine the levels of MDR-1 in a biops ...
Chapter 16 Molecular basis of inheritance
... synthesis of a new DNA strand. According to base-pairing rules, new nucleotides align along the template of the old DNA strand. DNA polymerase links the nucleotides to the growing strand in the 5’3’ direction. Hydrolysis of nucleoside phosphates provides the energy necessary to synthesize the new D ...
... synthesis of a new DNA strand. According to base-pairing rules, new nucleotides align along the template of the old DNA strand. DNA polymerase links the nucleotides to the growing strand in the 5’3’ direction. Hydrolysis of nucleoside phosphates provides the energy necessary to synthesize the new D ...
Fruitful DNA Extraction
... absence of a placenta, can be used to determine the evolutionary relationships between organisms. These morphological characters are part of an organism’s phenotype, which is any observable quality of an organism, such as its morphology or behavior. We can also use molecular characters to determine ...
... absence of a placenta, can be used to determine the evolutionary relationships between organisms. These morphological characters are part of an organism’s phenotype, which is any observable quality of an organism, such as its morphology or behavior. We can also use molecular characters to determine ...
T7 In Vitro Transcription Kit esiSCRIBE 100 Reactions (10 µl each
... sequence tag known to increase RNA yield). To obtain transcripts of defined length, linear template DNA should be used, which may be generated by linearization of plasmid DNA, by PCR or by hybridization of synthetic oligonucleotides. The kit is suitable for all applications that require T7 RNA polym ...
... sequence tag known to increase RNA yield). To obtain transcripts of defined length, linear template DNA should be used, which may be generated by linearization of plasmid DNA, by PCR or by hybridization of synthetic oligonucleotides. The kit is suitable for all applications that require T7 RNA polym ...
PowerPoint- Protein Shape
... 3) What are the steps of protein synthesis? 4) Compare transcription and translation. 5) How is DNA different from mRNA? HW: 1) Daily Review of class notes. 2) Textbook worksheet due Friday ...
... 3) What are the steps of protein synthesis? 4) Compare transcription and translation. 5) How is DNA different from mRNA? HW: 1) Daily Review of class notes. 2) Textbook worksheet due Friday ...
Deoxyribozyme
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Deoxyribozymes, also called DNA enzymes, DNAzymes, or catalytic DNA, are DNA oligonucleotides that are capable of catalyzing specific chemical reactions, similar to the action of other biological enzymes, such as proteins or ribozymes (enzymes composed of RNA).However, in contrast to the abundance of protein enzymes in biological systems and the discovery of biological ribozymes in the 1980s,there are no known naturally occurring deoxyribozymes.Deoxyribozymes should not be confused with DNA aptamers which are oligonucleotides that selectively bind a target ligand, but do not catalyze a subsequent chemical reaction.With the exception of ribozymes, nucleic acid molecules within cells primarily serve as storage of genetic information due to its ability to form complementary base pairs, which allows for high-fidelity copying and transfer of genetic information. In contrast, nucleic acid molecules are more limited in their catalytic ability, in comparison to protein enzymes, to just three types of interactions: hydrogen bonding, pi stacking, and metal-ion coordination. This is due to the limited number of functional groups of the nucleic acid monomers: while proteins are built from up to twenty different amino acids with various functional groups, nucleic acids are built from just four chemically similar nucleobases. In addition, DNA lacks the 2'-hydroxyl group found in RNA which limits the catalytic competency of deoxyribozymes even in comparison to ribozymes.In addition to the inherent inferiority of DNA catalytic activity, the apparent lack of naturally occurring deoxyribozymes may also be due to the primarily double-stranded conformation of DNA in biological systems which would limit its physical flexibility and ability to form tertiary structures, and so would drastically limit the ability of double-stranded DNA to act as a catalyst; though there are a few known instances of biological single-stranded DNA such as multicopy single-stranded DNA (msDNA), certain viral genomes, and the replication fork formed during DNA replication. Further structural differences between DNA and RNA may also play a role in the lack of biological deoxyribozymes, such as the additional methyl group of the DNA base thymidine compared to the RNA base uracil or the tendency of DNA to adopt the B-form helix while RNA tends to adopt the A-form helix. However, it has also been shown that DNA can form structures that RNA cannot, which suggests that, though there are differences in structures that each can form, neither is inherently more or less catalytic due to their possible structural motifs.