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DNA TEST
DNA TEST

... 18. The DNA of a certain organism has cytosine as 22% of its bases. What percentage of the bases are thymine? a) 28% b) 78% c) 50% d) 22% 19. Semi conservative replication means that a) Sometimes DNA can replicate and sometimes it cannot, this accounts for aging b) Sometimes newly made DNA molecules ...
genes: genetics, gemonics, an evolution
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... When preparing DNA inserts for a genomic library, you need to make sure that the fragments meet three criteria. Name any two. correct size for the vector of choice, regions of overlapping sequence among fragments, ends compatible with the vector. In order to prepare a concatomer for packaging in lam ...
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Microbial Genetics - Montgomery College

what is your dna alias
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... There are four nucleotide base molecules in DNA: Adenine, Cytosine, Guanine and Thymine. Three nucleotide bases together in a sequence on a DNA strand are called a ‘codon’. Because there are so many possible base sequences (i.e., codons), geneticists have developed a short-hand using our 26 letter a ...
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... (2) translation. Transcription is the process in which DNA is used as a template to produce a singlestranded RNA molecule. Translation is the process in which the DNA code, now contained in the singlestranded RNA, is deciphered into a sequence of linked amino acids that become a protein. In eukaryot ...
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... Each chromosome is made of many genes. Each gene is made up of a specific DNA sequence which codes for a specific amino acid sequence, otherwise called a protein. These proteins result in the presence or absence of particular traits, or phenotypes. The process of going from gene, or DNA, to protein ...
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... • Law of complimentary base pairing allows building of one DNA strand based on the bases in 2nd strand • Steps of replication process – DNA helicase opens short segment of helix • point of separation called replication fork ...
Chapter 8: Microbial Genetics 1. Gene Expression Gene Expression
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... Regulation of Transcription The focal point is whether or not RNA polymerase binds the promoter of a gene and initiates transcription which depends on: 1) Affinity of RNA polymerase for a given promoter • some promoters are “strong” and bind RNA polymerase with high affinity • some promoters are “we ...
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GENE to PROTEIN

... • Garrod (1909) hypothesized that the symptoms of an inherited disease reflect a person’s inability to make a particular enzyme. • The breakthrough in demonstrating the relationship between genes and enzymes when Beadle and Tatum began to search for mutants of bread mold. They discovered that mutan ...
GENE to PROTEIN
GENE to PROTEIN

... • Garrod (1909) hypothesized that the symptoms of an inherited disease reflect a person’s inability to make a particular enzyme. • The breakthrough in demonstrating the relationship between genes and enzymes when Beadle and Tatum began to search for mutants of bread mold. They discovered that mutan ...
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Deoxyribozyme



Deoxyribozymes, also called DNA enzymes, DNAzymes, or catalytic DNA, are DNA oligonucleotides that are capable of catalyzing specific chemical reactions, similar to the action of other biological enzymes, such as proteins or ribozymes (enzymes composed of RNA).However, in contrast to the abundance of protein enzymes in biological systems and the discovery of biological ribozymes in the 1980s,there are no known naturally occurring deoxyribozymes.Deoxyribozymes should not be confused with DNA aptamers which are oligonucleotides that selectively bind a target ligand, but do not catalyze a subsequent chemical reaction.With the exception of ribozymes, nucleic acid molecules within cells primarily serve as storage of genetic information due to its ability to form complementary base pairs, which allows for high-fidelity copying and transfer of genetic information. In contrast, nucleic acid molecules are more limited in their catalytic ability, in comparison to protein enzymes, to just three types of interactions: hydrogen bonding, pi stacking, and metal-ion coordination. This is due to the limited number of functional groups of the nucleic acid monomers: while proteins are built from up to twenty different amino acids with various functional groups, nucleic acids are built from just four chemically similar nucleobases. In addition, DNA lacks the 2'-hydroxyl group found in RNA which limits the catalytic competency of deoxyribozymes even in comparison to ribozymes.In addition to the inherent inferiority of DNA catalytic activity, the apparent lack of naturally occurring deoxyribozymes may also be due to the primarily double-stranded conformation of DNA in biological systems which would limit its physical flexibility and ability to form tertiary structures, and so would drastically limit the ability of double-stranded DNA to act as a catalyst; though there are a few known instances of biological single-stranded DNA such as multicopy single-stranded DNA (msDNA), certain viral genomes, and the replication fork formed during DNA replication. Further structural differences between DNA and RNA may also play a role in the lack of biological deoxyribozymes, such as the additional methyl group of the DNA base thymidine compared to the RNA base uracil or the tendency of DNA to adopt the B-form helix while RNA tends to adopt the A-form helix. However, it has also been shown that DNA can form structures that RNA cannot, which suggests that, though there are differences in structures that each can form, neither is inherently more or less catalytic due to their possible structural motifs.
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