Biology 445K Winter 2007 DNA Fingerprinting • For Friday 3/9 lab: in
... the genome that consist of repeated sequences. The repeat size is usually 10-60 base pairs long and the number of repeats varies from less than ten to several dozen. These sites, which are scattered throughout the genome, are usually “anonymous” markers in the sense that the repeat number does not a ...
... the genome that consist of repeated sequences. The repeat size is usually 10-60 base pairs long and the number of repeats varies from less than ten to several dozen. These sites, which are scattered throughout the genome, are usually “anonymous” markers in the sense that the repeat number does not a ...
Mendelian Genetics
... yellow seeds. Mendel found that some of their offspring had BOTH yellow and green seeds, with a ratio of 3:1. • Through these (and other) experiments Mendel devised several principles of inheritance. This is why today the study of genetic inheritance is called Mendelian ...
... yellow seeds. Mendel found that some of their offspring had BOTH yellow and green seeds, with a ratio of 3:1. • Through these (and other) experiments Mendel devised several principles of inheritance. This is why today the study of genetic inheritance is called Mendelian ...
Structure of Macromolecules Dr. Nakhshab
... The phosphate functional group has a negative electric charge, so this portion of the molecule is hydrophilic, attracting polar water molecules. But the two fatty acids are hydrophobic, so they tend to aggregate away ...
... The phosphate functional group has a negative electric charge, so this portion of the molecule is hydrophilic, attracting polar water molecules. But the two fatty acids are hydrophobic, so they tend to aggregate away ...
Mechanism of Surface Stress due to DNA strands on Gold
... about 1 billion molecules from the original one you started with!! ...
... about 1 billion molecules from the original one you started with!! ...
How the DNA Molecule Copies Itself
... Discovering the Structure of DNA • In order to understand how DNA functioned as the molecules that stored heredity, researchers needed to understand the structure of DNA DNA is comprised of subunits called nucleotides each DNA nucleotide has three parts 1. a central deoxyribose sugar 2. a phosp ...
... Discovering the Structure of DNA • In order to understand how DNA functioned as the molecules that stored heredity, researchers needed to understand the structure of DNA DNA is comprised of subunits called nucleotides each DNA nucleotide has three parts 1. a central deoxyribose sugar 2. a phosp ...
BMB 400 PART THREE - ANSWERS ANSWERS to Questions from
... tRNAMet, which can insert Met in interior positions in a polypeptide. tRNAfMet reacts with Met to yield Met-tRNAfMet, promoted by methionine aminoacyl-tRNA synthetase. The amino group of its Met residue is then formylated by N 10-formyltetrahydrofolate to yield fMettRNAfMet. Free Met or Met-tRNAMet ...
... tRNAMet, which can insert Met in interior positions in a polypeptide. tRNAfMet reacts with Met to yield Met-tRNAfMet, promoted by methionine aminoacyl-tRNA synthetase. The amino group of its Met residue is then formylated by N 10-formyltetrahydrofolate to yield fMettRNAfMet. Free Met or Met-tRNAMet ...
File
... Cells use the genetic information in DNA to produce proteins Proteins give rise to inherited genetic ...
... Cells use the genetic information in DNA to produce proteins Proteins give rise to inherited genetic ...
Transcription and Translation
... • The ribosomal unit binds to mRNA where the code for met is located (AUG). The anticodon (UAC) of the tRNA matches the “start” codon on mRNA (AUG). ...
... • The ribosomal unit binds to mRNA where the code for met is located (AUG). The anticodon (UAC) of the tRNA matches the “start” codon on mRNA (AUG). ...
DNA Replication and Protein_Synthesis
... DNA polymerase will only link an incoming nucleotide to the growing new chain if it is complementary to the base on the old strand. Very few mistakes are made, perhaps one in every 108 base pairs (1:1,000,000,000) ...
... DNA polymerase will only link an incoming nucleotide to the growing new chain if it is complementary to the base on the old strand. Very few mistakes are made, perhaps one in every 108 base pairs (1:1,000,000,000) ...
Chapter 9
... replication of DNA In your group, assign a step to each member Use pages 198 – 199 to describe your step in your own words Each member will also describe how DNA “checks for errors” Share each step in order once the whole group is finished ...
... replication of DNA In your group, assign a step to each member Use pages 198 – 199 to describe your step in your own words Each member will also describe how DNA “checks for errors” Share each step in order once the whole group is finished ...
AP Biology – Molecular Genetics (Chapters 14-17)
... 5. when RNA polymerase reaches a termination sequence, it leaves and so does the mRNA 6. three different types of RNA polymerase 7. pre-RNA (precursor RNA or transcript RNA) is made prior to mRNA which must be modified before forming mRNA that is exported out of the nucleus 8. snRNA (small nuclear R ...
... 5. when RNA polymerase reaches a termination sequence, it leaves and so does the mRNA 6. three different types of RNA polymerase 7. pre-RNA (precursor RNA or transcript RNA) is made prior to mRNA which must be modified before forming mRNA that is exported out of the nucleus 8. snRNA (small nuclear R ...
Structure of retroviruses
... During this period (of variable length but lasting on average about ...
... During this period (of variable length but lasting on average about ...
Techniques
... Techniques to know to understand signal transduction 1. __________- Detect DNA only 2. ___________- Detect RNA 3. ___________- Detect RNA of ____ of expressed genes 4. ________ ( Reverse transcription polymerase chain reaction- to detect RNA) 5. ________________________ Detect protein 6. __________ ...
... Techniques to know to understand signal transduction 1. __________- Detect DNA only 2. ___________- Detect RNA 3. ___________- Detect RNA of ____ of expressed genes 4. ________ ( Reverse transcription polymerase chain reaction- to detect RNA) 5. ________________________ Detect protein 6. __________ ...
Silke Alt
... Aminocoumarin antibiotics like clorobiocin and novobiocin produced by different Streptomyces strains are potent inhibitors of DNA gyrase. Although novobiocin has been licensed for clinical use in human infections with Gram-positive bacteria such as methicillin-resistant Staphylococcus aureus strains ...
... Aminocoumarin antibiotics like clorobiocin and novobiocin produced by different Streptomyces strains are potent inhibitors of DNA gyrase. Although novobiocin has been licensed for clinical use in human infections with Gram-positive bacteria such as methicillin-resistant Staphylococcus aureus strains ...
Gene Regulation - Biomedical Informatics
... positioned ~ -10bp upstream of TSS and TTGACA motif at ~ -35bp. 28. In eukaryotic promoters, the most important sequence elements are TATA-box at ~ -30bp and Initiator (Inr) situated on TSS. 29. There are multiple other promoter sequence motifs (cis-elements) which serve as substrates for trans-acti ...
... positioned ~ -10bp upstream of TSS and TTGACA motif at ~ -35bp. 28. In eukaryotic promoters, the most important sequence elements are TATA-box at ~ -30bp and Initiator (Inr) situated on TSS. 29. There are multiple other promoter sequence motifs (cis-elements) which serve as substrates for trans-acti ...
AP Biology DNA Technology: The manipulation of organisms or their
... A film is placed over the paper. The radioactive probe burns an image of the bands into the film. ...
... A film is placed over the paper. The radioactive probe burns an image of the bands into the film. ...
Tour of the Basics Web Quest
... 17. Does the second baby in the What is Heredity? animation inherit the exact same chromosomes as the first? Do both babies have a complete set? ...
... 17. Does the second baby in the What is Heredity? animation inherit the exact same chromosomes as the first? Do both babies have a complete set? ...
Chapter 8. Manipulating DNA, RNA and proteins
... -within the cell (in situ hybridization) -in tissue samples (northerns, DNA arrays) Tissues must be broken and the integrity of RNA preserved by inhibiting RNA degradation by RNases RNA is purified (several ways) and quantified by: -northern hybridization -reverse transcription -PCR (RT-PCR) -cDNA a ...
... -within the cell (in situ hybridization) -in tissue samples (northerns, DNA arrays) Tissues must be broken and the integrity of RNA preserved by inhibiting RNA degradation by RNases RNA is purified (several ways) and quantified by: -northern hybridization -reverse transcription -PCR (RT-PCR) -cDNA a ...
Detection and Measurement of Genetic Variation
... Is a technique that exploits variations in homologous DNA sequences. It refers to a difference between samples of homologous DNA molecules that come from differing locations of restriction enzyme sites. It took advantage of the existence of bacterial enzymes known as restriction endonucleases or res ...
... Is a technique that exploits variations in homologous DNA sequences. It refers to a difference between samples of homologous DNA molecules that come from differing locations of restriction enzyme sites. It took advantage of the existence of bacterial enzymes known as restriction endonucleases or res ...
stranded DNA from genomic library
... • Use of gel to separate DNA strands by size (molecular weight) or charge • DNA must first be “digested” – Strands must be cut into different sizes ...
... • Use of gel to separate DNA strands by size (molecular weight) or charge • DNA must first be “digested” – Strands must be cut into different sizes ...
Transcription and Translation Exercise
... 5. If a protein has 150 amino acids, how many DNA nucleotides would make up the coding region of the gene? ...
... 5. If a protein has 150 amino acids, how many DNA nucleotides would make up the coding region of the gene? ...
word doc - Southgate Schools
... Define each vocabulary term in your own words. Then, write yourself a quick note on how you will remember each. One term has been done for you. ...
... Define each vocabulary term in your own words. Then, write yourself a quick note on how you will remember each. One term has been done for you. ...
What do I have to know to feel confident and prepared for the DNA
... 10. How can we use biotechnology to predict the alleles for a lost person? We can use Short tandem repeats (STRs) in gel electrophoresis to separate the 2 alleles each person has. Once separated you can compare the position. If the alleles for two people are lined up at a set distance from the start ...
... 10. How can we use biotechnology to predict the alleles for a lost person? We can use Short tandem repeats (STRs) in gel electrophoresis to separate the 2 alleles each person has. Once separated you can compare the position. If the alleles for two people are lined up at a set distance from the start ...
Deoxyribozyme
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Deoxyribozymes, also called DNA enzymes, DNAzymes, or catalytic DNA, are DNA oligonucleotides that are capable of catalyzing specific chemical reactions, similar to the action of other biological enzymes, such as proteins or ribozymes (enzymes composed of RNA).However, in contrast to the abundance of protein enzymes in biological systems and the discovery of biological ribozymes in the 1980s,there are no known naturally occurring deoxyribozymes.Deoxyribozymes should not be confused with DNA aptamers which are oligonucleotides that selectively bind a target ligand, but do not catalyze a subsequent chemical reaction.With the exception of ribozymes, nucleic acid molecules within cells primarily serve as storage of genetic information due to its ability to form complementary base pairs, which allows for high-fidelity copying and transfer of genetic information. In contrast, nucleic acid molecules are more limited in their catalytic ability, in comparison to protein enzymes, to just three types of interactions: hydrogen bonding, pi stacking, and metal-ion coordination. This is due to the limited number of functional groups of the nucleic acid monomers: while proteins are built from up to twenty different amino acids with various functional groups, nucleic acids are built from just four chemically similar nucleobases. In addition, DNA lacks the 2'-hydroxyl group found in RNA which limits the catalytic competency of deoxyribozymes even in comparison to ribozymes.In addition to the inherent inferiority of DNA catalytic activity, the apparent lack of naturally occurring deoxyribozymes may also be due to the primarily double-stranded conformation of DNA in biological systems which would limit its physical flexibility and ability to form tertiary structures, and so would drastically limit the ability of double-stranded DNA to act as a catalyst; though there are a few known instances of biological single-stranded DNA such as multicopy single-stranded DNA (msDNA), certain viral genomes, and the replication fork formed during DNA replication. Further structural differences between DNA and RNA may also play a role in the lack of biological deoxyribozymes, such as the additional methyl group of the DNA base thymidine compared to the RNA base uracil or the tendency of DNA to adopt the B-form helix while RNA tends to adopt the A-form helix. However, it has also been shown that DNA can form structures that RNA cannot, which suggests that, though there are differences in structures that each can form, neither is inherently more or less catalytic due to their possible structural motifs.