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Document
Document

... makes copies of itself • It is subject to the laws of natural selection and must compete with other replicators for ...
RNA analysis on non-denaturing agarose gel electrophoresis
RNA analysis on non-denaturing agarose gel electrophoresis

... 2. Heat an aliquot of the RNA solution at 70°C for 1 min and place it on ice before loading on a gel. 3. Load a known amount of DNA or RNA ladder alongside your RNA sample as a standard for determining the RNA concentration. RNA concentration can be roughly estimated assuming that the efficiency of ...
humanvs
humanvs

... 1. What does it mean when scientists say that living organisms share a universal genetic code? It means that each living organism has a genetic code 2. How does a universal genetic code relate to the hypotheses about the origin of life on Earth?it is shared by all life forms on earth 3. How are self ...
Chapter 3 PowerPoint
Chapter 3 PowerPoint

... 4 Levels of structure 3. Tertiary structure – final folded shape of a globular protein – Stabilized by a number of forces – Final level of structure for proteins consisting of only a single polypeptide chain ...
4-5
4-5

LINEs
LINEs

... Non-autonomous (successful freeloaders! ‘borrow’ RT from other sources such as LINEs)  ~100-300bp long  Internal polymerase III promoter  No proteins  Share 3’ ends with LINEs  3 related SINE families in humans – active Alu, inactive MIR and Ther2/MIR3. ...
Zoo/Bot 3333
Zoo/Bot 3333

... a) 3.7 map units; b) 7.8 map units; c) 11 map units; d) 15.4 map units; e) 22 map units. Consider the gel at the right, derived from a sequencing reaction based on the Sanger chain termination method. A ...
PDF
PDF

... domain protein 2) and a co-factor [MBD3L1 (methyl-CpG binding domain protein 3-like 1)] to enrich for doublestranded methylated DNA that might have as few as two methyl groups. It should be noted that both of these techniques have been commercialized as kits, which might be useful to the novice user ...
Origin of life on Earth Two approaches: • bottom-up
Origin of life on Earth Two approaches: • bottom-up

sample exam 2010
sample exam 2010

... 76. Discuss the importance of 4 intermolecular and intramolecular forces in the secondary and tertiary structure of proteins. Name two ways these forces can be disrupted causing denaturation of the protein. (6 marks) 77. Describe the main idea behind the induced-fit model of enzyme activity. (2 mark ...
Chromosomes, genes, alleles and mutations
Chromosomes, genes, alleles and mutations

... already synthesized. ...
ProteinSynthesis
ProteinSynthesis

...  Segment of DNA that codes for the amino acid sequence in a protein are called genes ...
命題標頭紙 - 慈濟大學醫學資訊學系所
命題標頭紙 - 慈濟大學醫學資訊學系所

... 1. Briefly describe the central dogma of molecular biology (flow of genetic information). (10%) 2. Explain what are primary structure, secondary structure and tertiary structure of proteins. (10%) 3. A, T, G, and C are abbreviations for 4 amino acids. Write their full name and three letter codes, an ...
Chapter 6, Section 3: Advances in Genetics
Chapter 6, Section 3: Advances in Genetics

... The technique of inbreeding involves crossing two individuals that have similar characteristics.  For example, suppose a male and a female turkey are both plump and grow quickly. Their offspring will probably have those desirable qualities.  In bred organisms are genetically very similar and there ...
DNA Analysis
DNA Analysis

... • The probability of finding a particular base at one position can depend not only on the immediate adjacent bases, but also on several more distant bases upstream or downstream  higher order Markov model, (heterogeneous) • Gene finding: Markov models of coding and non-coding regions to classify se ...
Teacher Quality Grant - Gulf Coast State College
Teacher Quality Grant - Gulf Coast State College

... Items referring to the factors that affect enzyme activity are limited to concentration, pH, and temperature. Items will not require specific knowledge of how an enzyme reacts at a certain pH or temperature. Items will not assess the enzyme-substrate complex. ...
THE Macromolecules PowerPoint - Panhandle Area Educational
THE Macromolecules PowerPoint - Panhandle Area Educational

... Items referring to the factors that affect enzyme activity are limited to concentration, pH, and temperature. Items will not require specific knowledge of how an enzyme reacts at a certain pH or temperature. Items will not assess the enzyme-substrate complex. ...
Exercise 5. DNA Ligation, Selection and
Exercise 5. DNA Ligation, Selection and

... 50 bp long which contains 11 unique recognition sequences. An enzyme which recognizes one of these sequences will only cut at this site on the plasmid. The sequences within this stretch of DNA are collectively called multi-cloning or polycloning sites because they allow several different enzymes to ...
Do-It-Yourself Strawberry DNA
Do-It-Yourself Strawberry DNA

... DNA. The extraction of DNA from cells and its purification are of primary importance to the field of biotechnology and forensics. Extraction and purification of DNA are the first steps in the analysis and manipulation of DNA that allow scientists to detect genetic disorders, produce DNA fingerprints ...
Macromolecule Review
Macromolecule Review

... 2. Which of the molecules listed above can often be composed of C, H, and O alone? 3. Which of the compounds can be identified by looking at the C:H:O ratios alone? 4. What other elements are commonly associated with each of these four types of macromolecules? ...
Biology 3A Exam 3 Study Guide The exam will consist of multiple
Biology 3A Exam 3 Study Guide The exam will consist of multiple

Messenger RNA
Messenger RNA

... When Watson and Crick solved the double-helix structure of DNA they knew how DNA could be copied. All the cell had to do was separate the 2 strands and then use the base-pairing to make a new complementary strand for each. But… ...
The Only Way To Prove Macroevolution Is True
The Only Way To Prove Macroevolution Is True

... Let us consider another quote from Mr. Dawkins book in which he mentioned Lenski. Prior to the quote I am about to mention, he had talked about how much microevolution (without using the actual term) was able to physically change the appearance of animals. This is the quote: 'If so much evolutionary ...
Document
Document

... can they be reunited? If a body is found and the person cannot be identified by looks, how can we identify them? What types of identifiers do we have? ...
A. thaliana genotyping with a CAPS marker for a pks3
A. thaliana genotyping with a CAPS marker for a pks3

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Deoxyribozyme



Deoxyribozymes, also called DNA enzymes, DNAzymes, or catalytic DNA, are DNA oligonucleotides that are capable of catalyzing specific chemical reactions, similar to the action of other biological enzymes, such as proteins or ribozymes (enzymes composed of RNA).However, in contrast to the abundance of protein enzymes in biological systems and the discovery of biological ribozymes in the 1980s,there are no known naturally occurring deoxyribozymes.Deoxyribozymes should not be confused with DNA aptamers which are oligonucleotides that selectively bind a target ligand, but do not catalyze a subsequent chemical reaction.With the exception of ribozymes, nucleic acid molecules within cells primarily serve as storage of genetic information due to its ability to form complementary base pairs, which allows for high-fidelity copying and transfer of genetic information. In contrast, nucleic acid molecules are more limited in their catalytic ability, in comparison to protein enzymes, to just three types of interactions: hydrogen bonding, pi stacking, and metal-ion coordination. This is due to the limited number of functional groups of the nucleic acid monomers: while proteins are built from up to twenty different amino acids with various functional groups, nucleic acids are built from just four chemically similar nucleobases. In addition, DNA lacks the 2'-hydroxyl group found in RNA which limits the catalytic competency of deoxyribozymes even in comparison to ribozymes.In addition to the inherent inferiority of DNA catalytic activity, the apparent lack of naturally occurring deoxyribozymes may also be due to the primarily double-stranded conformation of DNA in biological systems which would limit its physical flexibility and ability to form tertiary structures, and so would drastically limit the ability of double-stranded DNA to act as a catalyst; though there are a few known instances of biological single-stranded DNA such as multicopy single-stranded DNA (msDNA), certain viral genomes, and the replication fork formed during DNA replication. Further structural differences between DNA and RNA may also play a role in the lack of biological deoxyribozymes, such as the additional methyl group of the DNA base thymidine compared to the RNA base uracil or the tendency of DNA to adopt the B-form helix while RNA tends to adopt the A-form helix. However, it has also been shown that DNA can form structures that RNA cannot, which suggests that, though there are differences in structures that each can form, neither is inherently more or less catalytic due to their possible structural motifs.
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