Cot-1 banding of human chromosomes using fluorescence
... (Fig. IA). There is high contrast between positive and negative bands. This Cot-1 banding image was recorded in a computer, and then Q-banding was performed on the same spread by conventional method (Fig. 1B). Then, these two fluorescent images of Cot-1 banding and Q-banding were computer-graphicall ...
... (Fig. IA). There is high contrast between positive and negative bands. This Cot-1 banding image was recorded in a computer, and then Q-banding was performed on the same spread by conventional method (Fig. 1B). Then, these two fluorescent images of Cot-1 banding and Q-banding were computer-graphicall ...
Repeat-induced point mutation and the population
... 19 of 30 sequences with three RIP mutations (P=0.035), and 23 of 44 sequences with four RIP mutations (P<0.001). Sequences with higher numbers of mutated RIP sites did not differ from chance expectation as to whether all the changes were only on one strand. These results may indicate that a small pe ...
... 19 of 30 sequences with three RIP mutations (P=0.035), and 23 of 44 sequences with four RIP mutations (P<0.001). Sequences with higher numbers of mutated RIP sites did not differ from chance expectation as to whether all the changes were only on one strand. These results may indicate that a small pe ...
Molecular identification of tick-borne pathogens in
... each dNTP. The conditions used for amplification were as follows: initial denaturing at 94 °C for 5 min, followed by 35 cycles of denaturation at 94 °C for 1 min, annealing at 55 °C for 1 min, and extension at 72 °C for 10 min. PCR-positive DNA samples were used to detect A. ovis, A. bovis, and A. p ...
... each dNTP. The conditions used for amplification were as follows: initial denaturing at 94 °C for 5 min, followed by 35 cycles of denaturation at 94 °C for 1 min, annealing at 55 °C for 1 min, and extension at 72 °C for 10 min. PCR-positive DNA samples were used to detect A. ovis, A. bovis, and A. p ...
doc
... A. Number of matches one can expect due to chance. B. Probability of obtaining a match of that quality due to chance. C. Number of standard deviations a match is above mean, generated by randomizing sequences. D. The measure derived from primary sequence similarity divided by the length of the matc ...
... A. Number of matches one can expect due to chance. B. Probability of obtaining a match of that quality due to chance. C. Number of standard deviations a match is above mean, generated by randomizing sequences. D. The measure derived from primary sequence similarity divided by the length of the matc ...
Biochimica et Biophysica Acta
... A bacterial two-plasmid system has been previously described for studying RNA–protein interactions [21]. In this system, a first plasmid contains a modified lacZ reporter gene with an RNA-binding element located close to the translation initiation region. A second plasmid expresses the RNA-binding pro ...
... A bacterial two-plasmid system has been previously described for studying RNA–protein interactions [21]. In this system, a first plasmid contains a modified lacZ reporter gene with an RNA-binding element located close to the translation initiation region. A second plasmid expresses the RNA-binding pro ...
Interfering RNA
... nucleic acid-based methods or other means and the gene sequence was known. • The current knowledge and level of skill in the art is high such that one of ordinary skill in the art would expect at least an RNAi against a known gene, absent evidence to the contrary. • Narrow claims to specific RNAi se ...
... nucleic acid-based methods or other means and the gene sequence was known. • The current knowledge and level of skill in the art is high such that one of ordinary skill in the art would expect at least an RNAi against a known gene, absent evidence to the contrary. • Narrow claims to specific RNAi se ...
Can epigenetics explain transgenerational transmission of acquired
... become locked in and inherited across multiple sexual generations, independently of any change in the genome sequence. This system of inheritance, called epigenetic, is best documented in plants and often involves differential DNA methylation of repeat sequences, notably transposable elements. Howev ...
... become locked in and inherited across multiple sexual generations, independently of any change in the genome sequence. This system of inheritance, called epigenetic, is best documented in plants and often involves differential DNA methylation of repeat sequences, notably transposable elements. Howev ...
IOSR Journal of Pharmacy and Biological Sciences (IOSR-JPBS) e-ISSN: 2278-3008.
... change may be a result of changes in the activity of enzymes. The activity of plant polyphenoloxidases can cause enzymatic browning of fruits and vegetables and render food unfit for consumption due to formation of benzoquinone. Proteases degrade polypeptides and facilitate seed germination, leaf se ...
... change may be a result of changes in the activity of enzymes. The activity of plant polyphenoloxidases can cause enzymatic browning of fruits and vegetables and render food unfit for consumption due to formation of benzoquinone. Proteases degrade polypeptides and facilitate seed germination, leaf se ...
method, a successful experiment must be verified by Southern blots
... purified DNA has provided simple, rapid methods for the molecular cloning of mutant forms of genes ("eviction" of mutant genes) and for the introduction into yeast of mutant genes constructed in vitro ("transplacement" of mutant genes). This chapter will place these techniques in a conceptual framew ...
... purified DNA has provided simple, rapid methods for the molecular cloning of mutant forms of genes ("eviction" of mutant genes) and for the introduction into yeast of mutant genes constructed in vitro ("transplacement" of mutant genes). This chapter will place these techniques in a conceptual framew ...
Complementary DNA Sequence of a Human Cytoplasmic Actin
... randomly distributed. but rather are clustered in specific regions (see Fig. 2). This suggests that certain segments of the actin sequence may be very crucial for filament formation. The percentage of nucleotides substituted within the coding regions of these sequences is significantly greater than ...
... randomly distributed. but rather are clustered in specific regions (see Fig. 2). This suggests that certain segments of the actin sequence may be very crucial for filament formation. The percentage of nucleotides substituted within the coding regions of these sequences is significantly greater than ...
Cloning and expression of proteins from Mycobacterium smegmatis
... these proteins are believed to be important for the cell membrane construction and therefore vital for the bacterial survival. New multi drug resistant (MDR) strains are developing in former Soviet Union countries. The antibiotic treatment is not completed and this creates a breeding ground for MDR- ...
... these proteins are believed to be important for the cell membrane construction and therefore vital for the bacterial survival. New multi drug resistant (MDR) strains are developing in former Soviet Union countries. The antibiotic treatment is not completed and this creates a breeding ground for MDR- ...
Section_11-3
... Cross-linkages are chemical bonds (as opposed to the physical attractions of intermolecular forces) Once formed, a chemical reaction must occur to break them (remember thermoset plastics!) Cross-linkages can change a liquid polymer into a solid or gel-like polymer ...
... Cross-linkages are chemical bonds (as opposed to the physical attractions of intermolecular forces) Once formed, a chemical reaction must occur to break them (remember thermoset plastics!) Cross-linkages can change a liquid polymer into a solid or gel-like polymer ...
Enzyme
... 2). Storage of enzymes Stored at high concentration, as lyophilized powders, or in a concentrated (NH4)2SO4 solution Some proteases may go through autolysis during storage. Some enzymes are easier to subject to denaturation at low concentrations. Stored at low temperatures Be careful: freez ...
... 2). Storage of enzymes Stored at high concentration, as lyophilized powders, or in a concentrated (NH4)2SO4 solution Some proteases may go through autolysis during storage. Some enzymes are easier to subject to denaturation at low concentrations. Stored at low temperatures Be careful: freez ...
Identiflcation and typing of grapevine phytoplasma amplified by graft
... 1993). In recent years, phytoplasma have mostly been diagnosed by polymerase chain reaction (PCR) with "universal" primers carrying phytoplasma-conserved sequences of the 16S ribosomal DNA (AHRENS and SEEMüLLER 1992; LEE etal. 1993; NAMBA etal. 1993 b; RASIN 1994; MAIXNER et al. 1995). These univers ...
... 1993). In recent years, phytoplasma have mostly been diagnosed by polymerase chain reaction (PCR) with "universal" primers carrying phytoplasma-conserved sequences of the 16S ribosomal DNA (AHRENS and SEEMüLLER 1992; LEE etal. 1993; NAMBA etal. 1993 b; RASIN 1994; MAIXNER et al. 1995). These univers ...
Peer-reviewed Article PDF
... bacteria S. thermophilus, which had just been sequenced, revealing an unusual CRISPR locus [4]. Although the CRISPR array was comparable to previously reported systems, it lacked some of the known Cas genes and instead contained unique Cas genes, including one encoding a large protein they predicted ...
... bacteria S. thermophilus, which had just been sequenced, revealing an unusual CRISPR locus [4]. Although the CRISPR array was comparable to previously reported systems, it lacked some of the known Cas genes and instead contained unique Cas genes, including one encoding a large protein they predicted ...
The wbbD gene of E. coli strain VW187
... catalyzed by the integral membrane protein WecA, a UDP-GlcNAc:Und-P GlcNAc-1-phosphate transferase that belongs to the family of polyisoprenyl-phosphate N-acetylhexosamine-1-phosphate transferases (Valvano, 2003). This family comprises proteins that are present both in prokaryotes and in eukaryotes ...
... catalyzed by the integral membrane protein WecA, a UDP-GlcNAc:Und-P GlcNAc-1-phosphate transferase that belongs to the family of polyisoprenyl-phosphate N-acetylhexosamine-1-phosphate transferases (Valvano, 2003). This family comprises proteins that are present both in prokaryotes and in eukaryotes ...
SBIOS_7_Y2 BIOL6024 Structural Biochemistry CA
... Name a reagent that can be used to detect the presence of reducing sugars (2 marks) ...
... Name a reagent that can be used to detect the presence of reducing sugars (2 marks) ...
Overview - BioMed Central
... pSANG4 replaces the pelB signal sequence of pHEN1/pSANG3 with the signal sequence from M13 gene 3. This leader is potentially more useful for ligation independent cloning (LIC). LIC which creates long single stranded overhangs and requires nucleotide stretches which use only 3 of the 4 bases (e.g. e ...
... pSANG4 replaces the pelB signal sequence of pHEN1/pSANG3 with the signal sequence from M13 gene 3. This leader is potentially more useful for ligation independent cloning (LIC). LIC which creates long single stranded overhangs and requires nucleotide stretches which use only 3 of the 4 bases (e.g. e ...
A survey of denitrifying Azospirillum brasilense in two contrasted
... reaction was carried out in a MJ Research Thermocycler. Amplification products were separated by electrophoresis at 4 V cm-1 in a 1.5 % agarose gel in TBE 0.5X running buffer. Gels were stained, washed and digitalized as previously explained. RAPD experiments were repeated three times to avoid false ...
... reaction was carried out in a MJ Research Thermocycler. Amplification products were separated by electrophoresis at 4 V cm-1 in a 1.5 % agarose gel in TBE 0.5X running buffer. Gels were stained, washed and digitalized as previously explained. RAPD experiments were repeated three times to avoid false ...
biojeopardy evolution
... setup was necessary to absorb most of the light’s infrared radiation while having little effect on the visible radiation. ...
... setup was necessary to absorb most of the light’s infrared radiation while having little effect on the visible radiation. ...
to the PDF file. - CURVE
... with a different mode of action. Trifluoromethionine (TFM) has been proposed as a potential novel antiinfective prodrug that is activated by the enzyme methionine γ-lyase (MGL), which is present in T. vaginalis but not in the cells of the human host. The presence of closely related enzymes, such as ...
... with a different mode of action. Trifluoromethionine (TFM) has been proposed as a potential novel antiinfective prodrug that is activated by the enzyme methionine γ-lyase (MGL), which is present in T. vaginalis but not in the cells of the human host. The presence of closely related enzymes, such as ...
Genetic mapping of Theobroma cacao (Malvaceae - Funpec-RP
... mapping and variability, and phylogeny studies of T. cacao (Lanaud et al., 1999). The availability of highly polymorphic neutral markers, allied to modern statistical procedures, allows the construction of linkage maps for most plant species, even perennials, such as forest and fruit trees (Carneiro ...
... mapping and variability, and phylogeny studies of T. cacao (Lanaud et al., 1999). The availability of highly polymorphic neutral markers, allied to modern statistical procedures, allows the construction of linkage maps for most plant species, even perennials, such as forest and fruit trees (Carneiro ...
Metabolism & Enzymes - T.R. Robinson High School
... 3-D structure of enzyme fits substrate substrate binding cause enzyme to change shape leading to a tighter fit ...
... 3-D structure of enzyme fits substrate substrate binding cause enzyme to change shape leading to a tighter fit ...
Revista agronomica del Noroeste Argentino
... reaction was carried out in a MJ Research Thermocycler. Amplification products were separated by electrophoresis at 4 V cm-1 in a 1.5 % agarose gel in TBE 0.5X running buffer. Gels were stained, washed and digitalized as previously explained. RAPD experiments were repeated three times to avoid false ...
... reaction was carried out in a MJ Research Thermocycler. Amplification products were separated by electrophoresis at 4 V cm-1 in a 1.5 % agarose gel in TBE 0.5X running buffer. Gels were stained, washed and digitalized as previously explained. RAPD experiments were repeated three times to avoid false ...
Rapid screening of PCR products using a novel agarose gel
... Fig 2. Comparison of separations on a large-format agarose gel vs. a Ready-To-Run gel. (A) 1 µl of a 1:10 dilution of PCR product separated in a 2% agarose gel containing SYBR Green I at 83 V for approximately 2 h. (B) 1 µl of a 1:5 dilution of PCR product separated in a 1.5% agarose Ready-To-Run ge ...
... Fig 2. Comparison of separations on a large-format agarose gel vs. a Ready-To-Run gel. (A) 1 µl of a 1:10 dilution of PCR product separated in a 2% agarose gel containing SYBR Green I at 83 V for approximately 2 h. (B) 1 µl of a 1:5 dilution of PCR product separated in a 1.5% agarose Ready-To-Run ge ...
Deoxyribozyme
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Deoxyribozymes, also called DNA enzymes, DNAzymes, or catalytic DNA, are DNA oligonucleotides that are capable of catalyzing specific chemical reactions, similar to the action of other biological enzymes, such as proteins or ribozymes (enzymes composed of RNA).However, in contrast to the abundance of protein enzymes in biological systems and the discovery of biological ribozymes in the 1980s,there are no known naturally occurring deoxyribozymes.Deoxyribozymes should not be confused with DNA aptamers which are oligonucleotides that selectively bind a target ligand, but do not catalyze a subsequent chemical reaction.With the exception of ribozymes, nucleic acid molecules within cells primarily serve as storage of genetic information due to its ability to form complementary base pairs, which allows for high-fidelity copying and transfer of genetic information. In contrast, nucleic acid molecules are more limited in their catalytic ability, in comparison to protein enzymes, to just three types of interactions: hydrogen bonding, pi stacking, and metal-ion coordination. This is due to the limited number of functional groups of the nucleic acid monomers: while proteins are built from up to twenty different amino acids with various functional groups, nucleic acids are built from just four chemically similar nucleobases. In addition, DNA lacks the 2'-hydroxyl group found in RNA which limits the catalytic competency of deoxyribozymes even in comparison to ribozymes.In addition to the inherent inferiority of DNA catalytic activity, the apparent lack of naturally occurring deoxyribozymes may also be due to the primarily double-stranded conformation of DNA in biological systems which would limit its physical flexibility and ability to form tertiary structures, and so would drastically limit the ability of double-stranded DNA to act as a catalyst; though there are a few known instances of biological single-stranded DNA such as multicopy single-stranded DNA (msDNA), certain viral genomes, and the replication fork formed during DNA replication. Further structural differences between DNA and RNA may also play a role in the lack of biological deoxyribozymes, such as the additional methyl group of the DNA base thymidine compared to the RNA base uracil or the tendency of DNA to adopt the B-form helix while RNA tends to adopt the A-form helix. However, it has also been shown that DNA can form structures that RNA cannot, which suggests that, though there are differences in structures that each can form, neither is inherently more or less catalytic due to their possible structural motifs.