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History of DNA
History of DNA

... genetics was discovered by Gregor Mendel. He found that ‘Factors’ determine the characteristics a living thing will express. The genes are passed to later generations, with a child taking genes from both its parents. The great mystery was where and how would this information be stored? ...
Hypothesis: Variations in the rate of DNA replication determine the
Hypothesis: Variations in the rate of DNA replication determine the

... The existence of two identical chromosomes within the same cell in which genes and higher order structures compete for limited resources is a symmetrybreaking situation previously proposed to lead to differentiation. Recent experiments are consistent with an intimate relationship between metabolism ...
MITOCHONDIAL GENETICS
MITOCHONDIAL GENETICS

... DNA polymerase can add free nucleotides to only the 3 end of the newly-forming strand. This results in elongation of the new strand in a 5'-3' direction. No known DNA polymerase is able to begin a new chain (de novo). DNA polymerase can add a nucleotide onto only a preexisting 3'-OH group, and, ther ...
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Concentration of solutes and solvent in a solution

... o Different functions of lipids:  function as long-term energy storage molecules  function as structural molecules-in cell membranes (phospholipids and cholesterol) Nucleic acids: o nucleotides, subunits of nucleotides o DNA and RNA o Store genetic information o Central dogma of molecular biology: ...
View ePoster - 2015 AGU Fall Meeting
View ePoster - 2015 AGU Fall Meeting

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MUTATION, DNA REPAIR AND CANCER

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copyright © adelaide tuition centre
copyright © adelaide tuition centre

... The complementary shape of the substrate’s active site and the enzyme ensure that binding can occur and the reaction will proceed. ...
MUTATION, DNA REPAIR AND CANCER
MUTATION, DNA REPAIR AND CANCER

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A Physiological Approach to DNA Music

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RT-PCR - Faperta UGM

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DNA and Proteins - Furman University
DNA and Proteins - Furman University

... remember, enzymes are proteins that catalyze reactions in the cell. In other words, enzymes are proteins that make stuff all this complex stuff with the energy in ATP. Enzymes make phospholipids, enzymes make sugars, enzymes make DNA, and enzymes make proteins. So, a fundamental question of how life ...
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7. Recombinant DNA Vectors

... artificial chromosome vectors (BACs, PACs, YACs)--cloning chromosomal regions b. Conventional E. coli plasmid cloning vectors typically have: origin of replication that functions in bacteria antibiotic resistance gene(s) selectable marker gene (often lacZ, encoding beta-galactosidase) polylinker (al ...
The Fossil Record
The Fossil Record

... 2. What is comparative anatomy? • The comparison of structures of different organisms • The more similar the anatomy, the more closely related the organisms are. ...
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Deoxyribozyme



Deoxyribozymes, also called DNA enzymes, DNAzymes, or catalytic DNA, are DNA oligonucleotides that are capable of catalyzing specific chemical reactions, similar to the action of other biological enzymes, such as proteins or ribozymes (enzymes composed of RNA).However, in contrast to the abundance of protein enzymes in biological systems and the discovery of biological ribozymes in the 1980s,there are no known naturally occurring deoxyribozymes.Deoxyribozymes should not be confused with DNA aptamers which are oligonucleotides that selectively bind a target ligand, but do not catalyze a subsequent chemical reaction.With the exception of ribozymes, nucleic acid molecules within cells primarily serve as storage of genetic information due to its ability to form complementary base pairs, which allows for high-fidelity copying and transfer of genetic information. In contrast, nucleic acid molecules are more limited in their catalytic ability, in comparison to protein enzymes, to just three types of interactions: hydrogen bonding, pi stacking, and metal-ion coordination. This is due to the limited number of functional groups of the nucleic acid monomers: while proteins are built from up to twenty different amino acids with various functional groups, nucleic acids are built from just four chemically similar nucleobases. In addition, DNA lacks the 2'-hydroxyl group found in RNA which limits the catalytic competency of deoxyribozymes even in comparison to ribozymes.In addition to the inherent inferiority of DNA catalytic activity, the apparent lack of naturally occurring deoxyribozymes may also be due to the primarily double-stranded conformation of DNA in biological systems which would limit its physical flexibility and ability to form tertiary structures, and so would drastically limit the ability of double-stranded DNA to act as a catalyst; though there are a few known instances of biological single-stranded DNA such as multicopy single-stranded DNA (msDNA), certain viral genomes, and the replication fork formed during DNA replication. Further structural differences between DNA and RNA may also play a role in the lack of biological deoxyribozymes, such as the additional methyl group of the DNA base thymidine compared to the RNA base uracil or the tendency of DNA to adopt the B-form helix while RNA tends to adopt the A-form helix. However, it has also been shown that DNA can form structures that RNA cannot, which suggests that, though there are differences in structures that each can form, neither is inherently more or less catalytic due to their possible structural motifs.
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