Gene Section RAD52 (RAD52 homolog (S. cerevisiae)) Atlas of Genetics and Cytogenetics
... Interfering with the Phe-79 of hRAD52 was recently demonstrated to disrupt the RAD52-DNA interaction leading to an accumulation of DNA double-strand breaks (DSBs) particularly in BRCA1/2 deficient cells (Cramer-Morales et al., ...
... Interfering with the Phe-79 of hRAD52 was recently demonstrated to disrupt the RAD52-DNA interaction leading to an accumulation of DNA double-strand breaks (DSBs) particularly in BRCA1/2 deficient cells (Cramer-Morales et al., ...
MS PowerPoint - Catalysis Eprints database
... Advantages of immobilization • stabilization of enzymes, as translational motion restricted in immobilized mode • Easy separation from reaction mixture, providing the ability to control reaction time. • minimize the enzymes lost in the product. • Re-use of enzymes for many reaction cycles, lowering ...
... Advantages of immobilization • stabilization of enzymes, as translational motion restricted in immobilized mode • Easy separation from reaction mixture, providing the ability to control reaction time. • minimize the enzymes lost in the product. • Re-use of enzymes for many reaction cycles, lowering ...
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... who were clinically diagnosed as DM1 were ruled out after molecular tests and they all also harbored two normal alleles when we used PCR to amplify CCTG repeats in the first intron of CNBP gene on chromosome 3q21. The false-positive of clinical diagnosis emphasizes again the importance of molecular ...
... who were clinically diagnosed as DM1 were ruled out after molecular tests and they all also harbored two normal alleles when we used PCR to amplify CCTG repeats in the first intron of CNBP gene on chromosome 3q21. The false-positive of clinical diagnosis emphasizes again the importance of molecular ...
Metabolism: Energy, Enzymes, and Regulation
... is so crucial to our understanding of microbial function, considerable attention is given to energy and enzymes in this chapter. The organization of microbial metabolism will be briefly described in chapters 8 to 10. Metabolic pathways are treated as a sequence of enzymes functioning as a unit, with ...
... is so crucial to our understanding of microbial function, considerable attention is given to energy and enzymes in this chapter. The organization of microbial metabolism will be briefly described in chapters 8 to 10. Metabolic pathways are treated as a sequence of enzymes functioning as a unit, with ...
A New Species of Actinomycete, Amycolata alni
... the substrate hyphae produced by a strain, especially on organic media, was very specific, so that the strains of Amycolata autotrophica produced orangish yellow vegetative mycelia, while the strains of Amycolata alni formed orangish brown to brown substrate mycelia. Chemotaxonomic properties. A stu ...
... the substrate hyphae produced by a strain, especially on organic media, was very specific, so that the strains of Amycolata autotrophica produced orangish yellow vegetative mycelia, while the strains of Amycolata alni formed orangish brown to brown substrate mycelia. Chemotaxonomic properties. A stu ...
Protein Folding
... • This is the “protein folding problem.” • The geometry and chemistry of the parts of the protein determine how it behaves in the cell. ...
... • This is the “protein folding problem.” • The geometry and chemistry of the parts of the protein determine how it behaves in the cell. ...
Modification of halogen specificity of a vanadium‐dependent
... (Barnett et al. 1998) have also been described. The amino acid sequence identities between vanadium BPOs and CPOs are low; however, the amino acids involved in vanadate coordination are highly conserved. The analyses of the three-dimensional structures of the enzymes from A. nodosum (Weyand et al. 1 ...
... (Barnett et al. 1998) have also been described. The amino acid sequence identities between vanadium BPOs and CPOs are low; however, the amino acids involved in vanadate coordination are highly conserved. The analyses of the three-dimensional structures of the enzymes from A. nodosum (Weyand et al. 1 ...
SI and non-SI units for Biological quantities
... Assignment of quantities to biological medicines: An old problem re-discovered Why have I told you this? TGN1412 is a super-agonist for the CD28 T-cell receptor. It is meant to stimulate release of T-cell cytokines It could be argued that qualitatively the response was not unexpected. What was unex ...
... Assignment of quantities to biological medicines: An old problem re-discovered Why have I told you this? TGN1412 is a super-agonist for the CD28 T-cell receptor. It is meant to stimulate release of T-cell cytokines It could be argued that qualitatively the response was not unexpected. What was unex ...
SensiFAST™ Two-Step Real-Time RT-PCR
... RT reaction is diluted into the real-time PCR in the second ...
... RT reaction is diluted into the real-time PCR in the second ...
bacterial plasmids - Acta Medica Medianae
... transfer, or oriT. This spot is asymmetric and it is oriented in such a way so genetic region, which controls the transfer, transfers at the end. OriT sequences differ among plasmids and they are very specific. For mobilization of non-conjugative plasmids, an interaction between theirs oriT sequence ...
... transfer, or oriT. This spot is asymmetric and it is oriented in such a way so genetic region, which controls the transfer, transfers at the end. OriT sequences differ among plasmids and they are very specific. For mobilization of non-conjugative plasmids, an interaction between theirs oriT sequence ...
Chapter 10 - People Server at UNCW
... • Production of protein from instructions on the DNA • Gene expression requires several steps • Transcription = Synthesizes an RNA molecule • Translation = Uses the information in the RNA to manufacture a protein by aligning and joining specified amino acids • Folding of the protein into specific 3- ...
... • Production of protein from instructions on the DNA • Gene expression requires several steps • Transcription = Synthesizes an RNA molecule • Translation = Uses the information in the RNA to manufacture a protein by aligning and joining specified amino acids • Folding of the protein into specific 3- ...
Mycobacterium kyorinense sp. nov., a novel, slow
... Sequence analysis revealed that the 16S rRNA gene sequence of the newly identified mycobacterium was closest to that of M. celatum ATCC 51130 (type 2) with 18 base mismatches out of 1469 bp (98.8 % identity). It was also highly similar to that of M. celatum NCTC 12882 (type 3) with 25 base mismatche ...
... Sequence analysis revealed that the 16S rRNA gene sequence of the newly identified mycobacterium was closest to that of M. celatum ATCC 51130 (type 2) with 18 base mismatches out of 1469 bp (98.8 % identity). It was also highly similar to that of M. celatum NCTC 12882 (type 3) with 25 base mismatche ...
Synthesis of Phosphopeptides Containing O
... (two with PSer and two with PThr) were synthesized by the same procedure and used as substratesfor casein kinase II (7). Seven different monophosphorylated peptides from the sequence of bovine rhodopsin have been made and tested as substrates for rhodopsin kinase (8). Although this method has been v ...
... (two with PSer and two with PThr) were synthesized by the same procedure and used as substratesfor casein kinase II (7). Seven different monophosphorylated peptides from the sequence of bovine rhodopsin have been made and tested as substrates for rhodopsin kinase (8). Although this method has been v ...
Ch16b: Peptides
... ‣ Proteins that are forced to straighten out loose many of the properties they demonstrate when their secondary structure is active. ‣ Understanding this secondary structure is an important part of understanding the nature of a particular protein. ‣ Chemists used to write out parts of amino acid seq ...
... ‣ Proteins that are forced to straighten out loose many of the properties they demonstrate when their secondary structure is active. ‣ Understanding this secondary structure is an important part of understanding the nature of a particular protein. ‣ Chemists used to write out parts of amino acid seq ...
Full report
... Of 9 ligands modelled in this entry, 3 are monoatomic - leaving 6 for Mogul analysis. In the following table, the Counts columns list the number of bonds (or angles) for which Mogul statistics could be retrieved, the number of bonds (or angles) that are observed in the model and the number of bonds ...
... Of 9 ligands modelled in this entry, 3 are monoatomic - leaving 6 for Mogul analysis. In the following table, the Counts columns list the number of bonds (or angles) for which Mogul statistics could be retrieved, the number of bonds (or angles) that are observed in the model and the number of bonds ...
Biology 101-003
... (including large and small subunits, E, P, and A sites). Know the structure and function of tRNA. Know where translation occurs if a protein is to be secreted and where it occurs if it is to remain within the cell. Be able to solve genetic code problems (from a given DNA sequence be able to determin ...
... (including large and small subunits, E, P, and A sites). Know the structure and function of tRNA. Know where translation occurs if a protein is to be secreted and where it occurs if it is to remain within the cell. Be able to solve genetic code problems (from a given DNA sequence be able to determin ...
Ch08-1enzymes
... molecules that reduce enzyme activity competitive inhibition noncompetitive inhibition irreversible inhibition feedback inhibition ...
... molecules that reduce enzyme activity competitive inhibition noncompetitive inhibition irreversible inhibition feedback inhibition ...
array CGH - Unique The Rare Chromosome Disorder Support Group
... and monitoring of your child’s health to know that the gene is present, absent or duplicated. It may also be reassuring to discover that such a gene is NOT included in your child’s duplication or deletion. However, it is important to remember that while identifying the gene(s) that are missing or du ...
... and monitoring of your child’s health to know that the gene is present, absent or duplicated. It may also be reassuring to discover that such a gene is NOT included in your child’s duplication or deletion. However, it is important to remember that while identifying the gene(s) that are missing or du ...
... Choice A: Describe the basic reaction mechanism for a typical DNA polymerase. Discuss why the Gibbs energy for the overall reaction is negative and also comment on the fidelity of the reaction, or why the polymerase is more likely to incorporate the correct base (Note: do not discuss removal of an i ...
Multiplex in-vitro Detection using SERS
... in the field of clinical diagnostics. The presence and progression of disease generally involves a multitude of different biomolecules; thus the detection of multiple events in tandem can reduce time and cost, as well as allowing significantly more information to be obtained from a small clinical sa ...
... in the field of clinical diagnostics. The presence and progression of disease generally involves a multitude of different biomolecules; thus the detection of multiple events in tandem can reduce time and cost, as well as allowing significantly more information to be obtained from a small clinical sa ...
Deoxyribozyme
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Deoxyribozymes, also called DNA enzymes, DNAzymes, or catalytic DNA, are DNA oligonucleotides that are capable of catalyzing specific chemical reactions, similar to the action of other biological enzymes, such as proteins or ribozymes (enzymes composed of RNA).However, in contrast to the abundance of protein enzymes in biological systems and the discovery of biological ribozymes in the 1980s,there are no known naturally occurring deoxyribozymes.Deoxyribozymes should not be confused with DNA aptamers which are oligonucleotides that selectively bind a target ligand, but do not catalyze a subsequent chemical reaction.With the exception of ribozymes, nucleic acid molecules within cells primarily serve as storage of genetic information due to its ability to form complementary base pairs, which allows for high-fidelity copying and transfer of genetic information. In contrast, nucleic acid molecules are more limited in their catalytic ability, in comparison to protein enzymes, to just three types of interactions: hydrogen bonding, pi stacking, and metal-ion coordination. This is due to the limited number of functional groups of the nucleic acid monomers: while proteins are built from up to twenty different amino acids with various functional groups, nucleic acids are built from just four chemically similar nucleobases. In addition, DNA lacks the 2'-hydroxyl group found in RNA which limits the catalytic competency of deoxyribozymes even in comparison to ribozymes.In addition to the inherent inferiority of DNA catalytic activity, the apparent lack of naturally occurring deoxyribozymes may also be due to the primarily double-stranded conformation of DNA in biological systems which would limit its physical flexibility and ability to form tertiary structures, and so would drastically limit the ability of double-stranded DNA to act as a catalyst; though there are a few known instances of biological single-stranded DNA such as multicopy single-stranded DNA (msDNA), certain viral genomes, and the replication fork formed during DNA replication. Further structural differences between DNA and RNA may also play a role in the lack of biological deoxyribozymes, such as the additional methyl group of the DNA base thymidine compared to the RNA base uracil or the tendency of DNA to adopt the B-form helix while RNA tends to adopt the A-form helix. However, it has also been shown that DNA can form structures that RNA cannot, which suggests that, though there are differences in structures that each can form, neither is inherently more or less catalytic due to their possible structural motifs.