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PartFourSumm_ThemesInRegulation.doc
PartFourSumm_ThemesInRegulation.doc

... 4. Some positive regulatory proteins (trans-activators) can increase the rate of transcription initiation by directly contacting the RNA polymerase and recruiting it to bind more avidly to the promoter. a. An example from eubacteria (E. coli) is the CAP protein, which in the presence of cAMP will bi ...
b. genetic engineering.
b. genetic engineering.

... DNA is separated from the other cell parts. • B. Cutting DNA- cut into small fragment by restriction enzymes (cut DNA at a specific sequence of nucleotides). • C. Separating DNA – method is gel ...
2. Organic Compounds and the Four Biomolec
2. Organic Compounds and the Four Biomolec

... A polypeptide is one linear chain of amino acids. A protein may contain one or more polypeptides. Proteins also sometimes contain small helper molecules such as heme. After the polypeptides are synthesized by the cell, they spontaneously fold up into a characteristic conformation which allows them t ...
2054, Chap. 13, page 1 I. Microbial Recombination and Plasmids
2054, Chap. 13, page 1 I. Microbial Recombination and Plasmids

... a. allows joining of DNA from different sources (e.g., phages, other bacteria, plasmids) b. most important for integration of viral genomes into bacterial chromosomes c. transposons use a type of site-specific recombination called replicative recombination 4. horizontal gene transfer = transfer of g ...
Biotechnology notes
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... leave “sticky ends” on both can glue DNA together at “sticky ends” ...
Honors Biology Chapter 3 Study Guide
Honors Biology Chapter 3 Study Guide

... b. What groups interact to form the tertiary structure?____________________________________ c. Beta pleated sheets make up what types of proteins?___________________________________ d. A tertiary structure is held together by the interactions of R groups plus ____________________ bonding, __________ ...
DNA Mutation
DNA Mutation

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Biology1FinalExam I F'04.doc
Biology1FinalExam I F'04.doc

... 8. The type of reaction commonly used to disconnect (break apart) the monomeric units of macromolecules is best classified as a/an: a. exergonic reaction. b. hydrolysis reaction. c. disulfide reaction. d. hydrogen bonding. e. dehydration reaction. 9. The actual sequence of amino acids in a polypepti ...
Forensic Science: Final Exam Review - Baxley
Forensic Science: Final Exam Review - Baxley

...  The Frye Standard: Expert opinion based on a scientific technique is admissible only where the technique is generally accepted as reliable in the relevant scientific community (The “General Acceptance Rule”)  The Daubert Standard: Scientific technique admissible in court if it is: o Testable o Pe ...
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... Most STRs occur in gene introns (non-coding regions of DNA) Does not usually affect gene function Can use as “markers” to differentiate between different alleles for certain genes (because genes located next to each other are inherited together.) ...
Week 5 - Reverse Transcriptase Polymerase Chain Reaction
Week 5 - Reverse Transcriptase Polymerase Chain Reaction

... same region are made, it stands out against the total genetic material of an organism. This allows specific genes and other regions to be studied in detail. RT-PCR (Reverse Transcriptase PCR) starts with total mRNA rather than DNA. This allows one to amplify and clone an expressed region of the geno ...
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Anatomy 10 Sample Questions (Exam One) Multiple Choice

... 10. Which of the following statements about phospholipids is TRUE? a. they are entirely hydrophobic b. they are the main source of energy in the body c. they have hydrophobic and hydrophilic regions d. they are important for energy storage in the body e. none of the above are true 11. The isotope 14 ...
Unit 3 Biochemistry - The Naked Science Society
Unit 3 Biochemistry - The Naked Science Society

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Poxvirus - rci.rutgers.edu
Poxvirus - rci.rutgers.edu

class syllabus
class syllabus

... 1. Distinguish between the following: (a). Genotype and Phenotype (b). heterozygous and homozygous (c). Dominant and recessive traits (d). Gene and allele 2. Jonathon, his sister Shelli and their maternal grandmother have a cleft chin. However, no one else in this family, including Jonathon and Shel ...
Prescott`s Microbiology, 9th Edition Chapter 18 – Microbial
Prescott`s Microbiology, 9th Edition Chapter 18 – Microbial

... Figure 18.1 What is the function of the 3’-OH during DNA synthesis? This is where the 5’ phosphate of the next nucleotide is covalently attached during elongation. Figure 18.4 Why is it important that the DNA to be sequenced is immobilized in all three of these techniques? With a flow cell, unless t ...
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amino acids biochemistry unit

... 5.1c: In all organisms, organic compounds can be used to assemble other molecules such as proteins, DNA, starch, and fats. The chemical energy stored in bonds can be used as a source of energy for life processes. ...
The Plant World and Genetic Engineering
The Plant World and Genetic Engineering

... Transgenic plants for phytoremediation Plant-derived plastics and polymers ...
20DNAtech - Mid
20DNAtech - Mid

... cell and transferred it into an infertile woman's egg. This material allowed the woman's egg to become fertile. The donor egg contained DNA from mitochondria, little organs inside the cell that create the energy to do life's work. The group believes that problems with the mitochondria prevented the ...
File - South Waksman Club
File - South Waksman Club

... • Modern cloning plasmids have been engineered so that they are incapable of transfer between bacterial cells • Provide a level of biological containment. ...
Supplementary Data 1 (doc 909K)
Supplementary Data 1 (doc 909K)

... PM/MM model for gene expression (dChip, www.dchip.org). Utilizing the P, M, and A calls, genes that did not exhibit at least 75% P or M calls in at least one of the groups of the categorical variable being examined were screened out. Following this, the Kruskal-Wallis non-parametric one-way analysis ...
File
File

... determine whether suspects have been at a crime scene. In the past ten years, biotechnologists have developed a method that determines DNA fingerprints. DNA fingerprinting can be used to convict or acquit individuals of criminal offenses because every person is genetically unique. d. It is also poss ...
http://ict.aiias.edu/vol_26A/26Acc_271-290.pdf
http://ict.aiias.edu/vol_26A/26Acc_271-290.pdf

... In spite of the existence of some exceptions to the absence of transition links that were not discussed here, it can be observed that the general picture presented in the fossil record favors creation model. In this model, God created separately the groups of animals, as described in the book of Gen ...
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Deoxyribozyme



Deoxyribozymes, also called DNA enzymes, DNAzymes, or catalytic DNA, are DNA oligonucleotides that are capable of catalyzing specific chemical reactions, similar to the action of other biological enzymes, such as proteins or ribozymes (enzymes composed of RNA).However, in contrast to the abundance of protein enzymes in biological systems and the discovery of biological ribozymes in the 1980s,there are no known naturally occurring deoxyribozymes.Deoxyribozymes should not be confused with DNA aptamers which are oligonucleotides that selectively bind a target ligand, but do not catalyze a subsequent chemical reaction.With the exception of ribozymes, nucleic acid molecules within cells primarily serve as storage of genetic information due to its ability to form complementary base pairs, which allows for high-fidelity copying and transfer of genetic information. In contrast, nucleic acid molecules are more limited in their catalytic ability, in comparison to protein enzymes, to just three types of interactions: hydrogen bonding, pi stacking, and metal-ion coordination. This is due to the limited number of functional groups of the nucleic acid monomers: while proteins are built from up to twenty different amino acids with various functional groups, nucleic acids are built from just four chemically similar nucleobases. In addition, DNA lacks the 2'-hydroxyl group found in RNA which limits the catalytic competency of deoxyribozymes even in comparison to ribozymes.In addition to the inherent inferiority of DNA catalytic activity, the apparent lack of naturally occurring deoxyribozymes may also be due to the primarily double-stranded conformation of DNA in biological systems which would limit its physical flexibility and ability to form tertiary structures, and so would drastically limit the ability of double-stranded DNA to act as a catalyst; though there are a few known instances of biological single-stranded DNA such as multicopy single-stranded DNA (msDNA), certain viral genomes, and the replication fork formed during DNA replication. Further structural differences between DNA and RNA may also play a role in the lack of biological deoxyribozymes, such as the additional methyl group of the DNA base thymidine compared to the RNA base uracil or the tendency of DNA to adopt the B-form helix while RNA tends to adopt the A-form helix. However, it has also been shown that DNA can form structures that RNA cannot, which suggests that, though there are differences in structures that each can form, neither is inherently more or less catalytic due to their possible structural motifs.
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