ELECTROPHORESIS
... change from a compound to another depending on the compound chemical nature and size. The technique is implemented for various uses: 1- The identification of certain molecules. 2- The isolation of a certain molecule. 3- The molecular weight of certain molecules. In this lecture, Polyacrylamide Gel E ...
... change from a compound to another depending on the compound chemical nature and size. The technique is implemented for various uses: 1- The identification of certain molecules. 2- The isolation of a certain molecule. 3- The molecular weight of certain molecules. In this lecture, Polyacrylamide Gel E ...
Darwin`s finches - University of Birmingham
... Scientists at this time were treating species as fixed entities and there was not yet a suggestion that one species could ‘evolve’ into another (or that more closely related species had more recent common ancestors). ...
... Scientists at this time were treating species as fixed entities and there was not yet a suggestion that one species could ‘evolve’ into another (or that more closely related species had more recent common ancestors). ...
macromolecules
... The Role of Carbon in Organisms: • Carbon compounds that come from living organisms are called organic compounds. • Two carbon atoms can form various types of covalent bonds—single, double or triple. ...
... The Role of Carbon in Organisms: • Carbon compounds that come from living organisms are called organic compounds. • Two carbon atoms can form various types of covalent bonds—single, double or triple. ...
Biocatalysis in Organic Synthesis
... Chemoselectivity: Since the purpose of an enzyme is to act on a single type of functional group, other sensitive functionalities, which would normally react to a certain extent under chemical catalysis, survive. As a result, biocatalytic reactions tend to be "cleaner" and laborious purification of p ...
... Chemoselectivity: Since the purpose of an enzyme is to act on a single type of functional group, other sensitive functionalities, which would normally react to a certain extent under chemical catalysis, survive. As a result, biocatalytic reactions tend to be "cleaner" and laborious purification of p ...
Chap 8 Recombinant DNA technology Fall 2012
... of cDNA – Easier to isolate mRNA molecule for desired protein first – mRNA of eukaryotes has introns removed – Allows cloning in prokaryotic cells ...
... of cDNA – Easier to isolate mRNA molecule for desired protein first – mRNA of eukaryotes has introns removed – Allows cloning in prokaryotic cells ...
Chapter 5A
... Plasmids are autonomously replicating circular DNAs found in bacterial cells. Naturally occurring plasmids contain an origin of replication (ori) for propagation in the host cell and one or more genes that specify a trait that may be useful to the host. Cloning vectors are plasmids that have been ge ...
... Plasmids are autonomously replicating circular DNAs found in bacterial cells. Naturally occurring plasmids contain an origin of replication (ori) for propagation in the host cell and one or more genes that specify a trait that may be useful to the host. Cloning vectors are plasmids that have been ge ...
Title goes here
... skeleton of reactions, which consist of reactants (compounds) • All reactions share the common base of proteins annotated as catalysts • Can we merge the information from different collections, using the best features of all of them? ...
... skeleton of reactions, which consist of reactants (compounds) • All reactions share the common base of proteins annotated as catalysts • Can we merge the information from different collections, using the best features of all of them? ...
Methods in Molecular Biology 1297: RNA Nanotechnology and
... The target readership for this Methods book includes practitioners already involved in RNA nanotechnology and scientists who are considering starting research in this area. As a collection of detailed laboratory protocols, the introductory description of context and background in each chapter is exp ...
... The target readership for this Methods book includes practitioners already involved in RNA nanotechnology and scientists who are considering starting research in this area. As a collection of detailed laboratory protocols, the introductory description of context and background in each chapter is exp ...
1 Biology - Organic Chemistry Notes Name Date Organic Chemistry
... proteins. Protein molecules are long, usually folded chains made from 20 different kinds of amino acids in a specific sequence. This sequence influences the shape of the protein. The shape of the protein, in tern determines its function. -------------------------------------------------------------- ...
... proteins. Protein molecules are long, usually folded chains made from 20 different kinds of amino acids in a specific sequence. This sequence influences the shape of the protein. The shape of the protein, in tern determines its function. -------------------------------------------------------------- ...
Molecular markers
... The ligation of double-stranded (ds) adapters to the ends of the restriction fragments The amplification of a subset of the restriction fragments using two primers complementary to the adapter and restriction site sequences, and extended at their 3' ends by "selective nucleotides" ...
... The ligation of double-stranded (ds) adapters to the ends of the restriction fragments The amplification of a subset of the restriction fragments using two primers complementary to the adapter and restriction site sequences, and extended at their 3' ends by "selective nucleotides" ...
Organic Molecules
... • There are two families of nitrogenous bases, pyrimidines and purines. Pyrimidines have a six-membered ring. Pyrimidines include: cytosine (C) (in DNA and RNA), thymine (T)(only in DNA), and uracil (U)(only in RNA). The purines adenine (A) and guanine (G) are larger with a six-membered ring attache ...
... • There are two families of nitrogenous bases, pyrimidines and purines. Pyrimidines have a six-membered ring. Pyrimidines include: cytosine (C) (in DNA and RNA), thymine (T)(only in DNA), and uracil (U)(only in RNA). The purines adenine (A) and guanine (G) are larger with a six-membered ring attache ...
Genetic Engineering
... Plant Breeding • Drugs used in plant breeding sometimes cause plants to produce cells that have double or triple the normal number of chromosomes. • Plants grown from such cells are called polyploid because they have many sets of chromosomes. • Polyploidy produces larger and stronger plants, which ...
... Plant Breeding • Drugs used in plant breeding sometimes cause plants to produce cells that have double or triple the normal number of chromosomes. • Plants grown from such cells are called polyploid because they have many sets of chromosomes. • Polyploidy produces larger and stronger plants, which ...
Lecture8-Chap5 Sept26
... alleles in a defined region of some chromosome; in effect, the genotype in miniature. – Originally used to describe combinations of major histocompatibility complex (MHC) alleles, it now may be used to describe particular combinations of RFLPs, SNPs, or other markers. ...
... alleles in a defined region of some chromosome; in effect, the genotype in miniature. – Originally used to describe combinations of major histocompatibility complex (MHC) alleles, it now may be used to describe particular combinations of RFLPs, SNPs, or other markers. ...
Biology 105 Midterm Exam 2
... c. It organizes evidence and helps us predict what will happen in our environment. d. It distinguishes between good and bad. 5. The scientific method can be used for other things besides science. a. true b. false 6. This question refers to an experiment whose results are summarized in the table belo ...
... c. It organizes evidence and helps us predict what will happen in our environment. d. It distinguishes between good and bad. 5. The scientific method can be used for other things besides science. a. true b. false 6. This question refers to an experiment whose results are summarized in the table belo ...
Biomolecules PPT
... Examples – meats, nuts and beans, fish •Makes muscle, feathers, hair and nails and enzymes •An enzyme is a molecule that speeds up or slows down a chemical reaction so that it can occur at body temperature. ...
... Examples – meats, nuts and beans, fish •Makes muscle, feathers, hair and nails and enzymes •An enzyme is a molecule that speeds up or slows down a chemical reaction so that it can occur at body temperature. ...
Lecture8-Chap5 Sept26
... alleles in a defined region of some chromosome; in effect, the genotype in miniature. – Originally used to describe combinations of major histocompatibility complex (MHC) alleles, it now may be used to describe particular combinations of RFLPs, SNPs, or other markers. ...
... alleles in a defined region of some chromosome; in effect, the genotype in miniature. – Originally used to describe combinations of major histocompatibility complex (MHC) alleles, it now may be used to describe particular combinations of RFLPs, SNPs, or other markers. ...
DNA Fingerprinting
... The Human Genome Project has provided information to link the identification of many types of cancers and other diseases to DNKA sequence information. (Edvotek) Cancer has been found to be linked to mutations in a tumor suppressor genes such as one called p53. These genes usually keep cells from div ...
... The Human Genome Project has provided information to link the identification of many types of cancers and other diseases to DNKA sequence information. (Edvotek) Cancer has been found to be linked to mutations in a tumor suppressor genes such as one called p53. These genes usually keep cells from div ...
DNA Clean/Extraction Kit
... Three different applications (PCR clean-up, DNA clean-up, gel extraction) with the same solutions. Up to 150 μl DNA solution or PCR reaction can be processed through each single Spin Column, from which 80%~95% of applied DNA can be ...
... Three different applications (PCR clean-up, DNA clean-up, gel extraction) with the same solutions. Up to 150 μl DNA solution or PCR reaction can be processed through each single Spin Column, from which 80%~95% of applied DNA can be ...
Replication of chromosomal DNA
... generate Hfr (high frequency of recombination) donors Excision of F plasmid can produce a recombinant F plasmid (F’) which contains a fragment of bacterial chromosomal DNA ...
... generate Hfr (high frequency of recombination) donors Excision of F plasmid can produce a recombinant F plasmid (F’) which contains a fragment of bacterial chromosomal DNA ...
Sal I (R0754) - Datasheet - Sigma
... N6-methyladenine at GTCGmAC. Sal I exhibits star activity under non-optimal conditions. 100 units of Sal I can be heat inactivated after 15 minutes at 65 °C. Sal I Storage and Dilution Buffer: 10 mM Tris-HCl, 1.0 mM EDTA, 10 mM dithioerythritol, and 50% (v/v) glycerol, pH 7.5 Activity: 10,000 units/ ...
... N6-methyladenine at GTCGmAC. Sal I exhibits star activity under non-optimal conditions. 100 units of Sal I can be heat inactivated after 15 minutes at 65 °C. Sal I Storage and Dilution Buffer: 10 mM Tris-HCl, 1.0 mM EDTA, 10 mM dithioerythritol, and 50% (v/v) glycerol, pH 7.5 Activity: 10,000 units/ ...
Deoxyribozyme
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Deoxyribozymes, also called DNA enzymes, DNAzymes, or catalytic DNA, are DNA oligonucleotides that are capable of catalyzing specific chemical reactions, similar to the action of other biological enzymes, such as proteins or ribozymes (enzymes composed of RNA).However, in contrast to the abundance of protein enzymes in biological systems and the discovery of biological ribozymes in the 1980s,there are no known naturally occurring deoxyribozymes.Deoxyribozymes should not be confused with DNA aptamers which are oligonucleotides that selectively bind a target ligand, but do not catalyze a subsequent chemical reaction.With the exception of ribozymes, nucleic acid molecules within cells primarily serve as storage of genetic information due to its ability to form complementary base pairs, which allows for high-fidelity copying and transfer of genetic information. In contrast, nucleic acid molecules are more limited in their catalytic ability, in comparison to protein enzymes, to just three types of interactions: hydrogen bonding, pi stacking, and metal-ion coordination. This is due to the limited number of functional groups of the nucleic acid monomers: while proteins are built from up to twenty different amino acids with various functional groups, nucleic acids are built from just four chemically similar nucleobases. In addition, DNA lacks the 2'-hydroxyl group found in RNA which limits the catalytic competency of deoxyribozymes even in comparison to ribozymes.In addition to the inherent inferiority of DNA catalytic activity, the apparent lack of naturally occurring deoxyribozymes may also be due to the primarily double-stranded conformation of DNA in biological systems which would limit its physical flexibility and ability to form tertiary structures, and so would drastically limit the ability of double-stranded DNA to act as a catalyst; though there are a few known instances of biological single-stranded DNA such as multicopy single-stranded DNA (msDNA), certain viral genomes, and the replication fork formed during DNA replication. Further structural differences between DNA and RNA may also play a role in the lack of biological deoxyribozymes, such as the additional methyl group of the DNA base thymidine compared to the RNA base uracil or the tendency of DNA to adopt the B-form helix while RNA tends to adopt the A-form helix. However, it has also been shown that DNA can form structures that RNA cannot, which suggests that, though there are differences in structures that each can form, neither is inherently more or less catalytic due to their possible structural motifs.