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Microevolution Notes
Microevolution Notes

... Every individual in a population is different in structure and behavior. Ex-pattern, speed, agility, etc ...
Evolution of prokaryotic genomes
Evolution of prokaryotic genomes

... involved in conjuration. These belong to the small autonphenotypic traits or not. omously replicating DNA molecules called plasmids. It It is a long experience of geneticists that mutations is interesting to note that these three basic processes of may often be lethal so that the mutant cannot under ...
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Synthesis and Characterization of Peptide Nucleic Acid for

... specific region of DNA. As a result, these AP-PNA adducts may display qualities favorable for prolonged gene-silencing or gene-activation. Our PNA was designed to compliment the T7 promoter sequence of PBluescriptKII and thereby bind to the DNA in vitro. The synthesis of the PNA was carried out usin ...
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Everything you wanted to know about ENCODE

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... of the codon) often do not change the resulting amino acid. See the code table. Therefore it is possible to change the genotype (the DNA) without changing the phenotype (the function or appearance). B-2. AUG to AUA is missense – it changes the amino acid from met to ile. UAC to UAA causes a change b ...
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... 29. Which of the following statements about protein synthesis is correct? (A) Protein synthesis stops at the amino end. (B) Transcription of mRNAs and translation into proteins are uncoupled in most eukaryotic systems. (C) Protein synthesis proceeds in the 3’ to 5’ direction of the mRNA. (D) Amino a ...
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... Scientists can now synthesise short one-sided pieces of DNA, called oligonucleotides. These are made by machines from a computer program. These oligonucleotides are used as: Primers for the Polymerase Chain Reaction (PCR). They do this by providing an attachment point for DNA polymerase to synthesis ...
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... occurred. Usually during digestion, there is a release of heat energy when chemical bonds are broken, so the temperature should increase. This is called an exothermic reaction. Once the initial temperature readings have been recorded, use a popsicle stick to slide a piece of liver from a plate into ...
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Chapter 19 - Microbiology and Molecular Genetics at Oklahoma

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Effect of defects on thermal denaturation of DNA Oligomers

... chains of heterogeneous compositions. A defect on DNA chain means a mismatched basepair. For example, if one strand of DNA has adenine on a site the other strand has guanine or cytosine instead of thymine on the same site. In such a situation the pair will remain in open state at all temperatures as ...
Studying the Embryo Lethality of AT5G03220
Studying the Embryo Lethality of AT5G03220

... All ten samples displayed the presence of at least one Wild Type Allele, and through T-DNA specific PCR, it was verified that all of the plants were homozygous for the Wild Type allele. So far, results obtained suggests the high possibility that the SALK 109178 insertion may cause embryo-lethality i ...
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Deoxyribozyme



Deoxyribozymes, also called DNA enzymes, DNAzymes, or catalytic DNA, are DNA oligonucleotides that are capable of catalyzing specific chemical reactions, similar to the action of other biological enzymes, such as proteins or ribozymes (enzymes composed of RNA).However, in contrast to the abundance of protein enzymes in biological systems and the discovery of biological ribozymes in the 1980s,there are no known naturally occurring deoxyribozymes.Deoxyribozymes should not be confused with DNA aptamers which are oligonucleotides that selectively bind a target ligand, but do not catalyze a subsequent chemical reaction.With the exception of ribozymes, nucleic acid molecules within cells primarily serve as storage of genetic information due to its ability to form complementary base pairs, which allows for high-fidelity copying and transfer of genetic information. In contrast, nucleic acid molecules are more limited in their catalytic ability, in comparison to protein enzymes, to just three types of interactions: hydrogen bonding, pi stacking, and metal-ion coordination. This is due to the limited number of functional groups of the nucleic acid monomers: while proteins are built from up to twenty different amino acids with various functional groups, nucleic acids are built from just four chemically similar nucleobases. In addition, DNA lacks the 2'-hydroxyl group found in RNA which limits the catalytic competency of deoxyribozymes even in comparison to ribozymes.In addition to the inherent inferiority of DNA catalytic activity, the apparent lack of naturally occurring deoxyribozymes may also be due to the primarily double-stranded conformation of DNA in biological systems which would limit its physical flexibility and ability to form tertiary structures, and so would drastically limit the ability of double-stranded DNA to act as a catalyst; though there are a few known instances of biological single-stranded DNA such as multicopy single-stranded DNA (msDNA), certain viral genomes, and the replication fork formed during DNA replication. Further structural differences between DNA and RNA may also play a role in the lack of biological deoxyribozymes, such as the additional methyl group of the DNA base thymidine compared to the RNA base uracil or the tendency of DNA to adopt the B-form helix while RNA tends to adopt the A-form helix. However, it has also been shown that DNA can form structures that RNA cannot, which suggests that, though there are differences in structures that each can form, neither is inherently more or less catalytic due to their possible structural motifs.
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