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Non-coding RNA | Principles of Biology from Nature Education
Non-coding RNA | Principles of Biology from Nature Education

... enhancer RNA (eRNA) is made by enhancer regions in genes and appears to amplify protein production when the genes associated with the enhancers are expressed. Scientists have long known that enhancer regions bind with transcription factors to enhance the transcription of targeted genes by interacti ...
recombinant DNA - juan
recombinant DNA - juan

... • The remarkable ability of bacteria to express some eukaryotic proteins underscores the shared evolutionary ancestry of living species • For example, Pax-6 is a gene that directs formation of a vertebrate eye; the same gene in flies directs the formation of an insect eye (which is quite different f ...
Design of a High School Laboratory: `Visualizing DNA Sequences`
Design of a High School Laboratory: `Visualizing DNA Sequences`

... research was made into teaching techniques to determine which would be effective in teaching Bioinformatics and other lab manuals written for Bioinformatics and Genomics were studied. Before the final version of the lab was designed, feedback was sought from individuals who had reviewed and/or used ...
Ch 20 Biotechnology - juan-roldan
Ch 20 Biotechnology - juan-roldan

... • The remarkable ability of bacteria to express some eukaryotic proteins underscores the shared evolutionary ancestry of living species • For example, Pax-6 is a gene that directs formation of a vertebrate eye; the same gene in flies directs the formation of an insect eye (which is quite different f ...
Lehninger Principles of Biochemistry
Lehninger Principles of Biochemistry

CHAPTER 8 Applications of Recombinant DNA Technology
CHAPTER 8 Applications of Recombinant DNA Technology

... 4. PCR is another approach to DNA molecular testing. It requires sequence information so that specific primers can be designed. An example is allele-specific oligonucleotide (ASO) hybridization used to detect mutations in GLC1A, a gene involved in maintaining normal eye pressure. a. Abnormal pressur ...
Chapter 5 Notes Bio AP
Chapter 5 Notes Bio AP

... Most animal fats are saturated. Saturated fats are solid at room temperature. Plant and fish fats are liquid at room temperature and are known as oils. The kinks caused by the cis double bonds prevent the molecules from packing tightly enough to solidify at room temperature. The phrase “hydrogenated ...
published a paper
published a paper

C483 Study Guide for Exam 1 Summer 2016 Basic Information
C483 Study Guide for Exam 1 Summer 2016 Basic Information

... domains, thermodynamics of protein folding, size exclusion chromatography, ion-exchange chromatography, affinity chromatography, SDS-PAGE, isoelectric focusing, Edman Degradation, partial digestion, myoglobin/hemoglobin structure-function, oxygen binding curve, hyperbolic vs sigmoidal curves, cooper ...
Consalez, GG, Stayton, CL, Freimer, NB, Goonewardena, Brown, WT, Gilliam, TC and Warren, ST: Isolation and characterization of a highly polymorphic human locus (DXS 455) in proximal Xq28. Genomics 12:710-714 (1992).
Consalez, GG, Stayton, CL, Freimer, NB, Goonewardena, Brown, WT, Gilliam, TC and Warren, ST: Isolation and characterization of a highly polymorphic human locus (DXS 455) in proximal Xq28. Genomics 12:710-714 (1992).

... 8.2 kb (7.8-8.6 kb). Since the allele size variation is relatively small, enhanced resolution of the fragment sizes is achieved by long electrophoresis times, as described above. As shown in Table 1, the combined heterozygosity for both DXS455 probes (~346.8 and p346.T) in 21 unrelated females of mi ...
Chemical Substitutes - UC Davis Safety Services
Chemical Substitutes - UC Davis Safety Services

... Chemical Substitutes This list suggests chemical substitutes for commonly used chemicals to promote a safer work environment. Consider using the safer substitute if it works in your process: Original Material ...
Epstein-Barr virus DNA in serum in a HIV
Epstein-Barr virus DNA in serum in a HIV

... 8, 9] showing a similar sensitivity, but higher specificity than its levels in PBMC from the same patients [8]. Although these findings were not confirmed in other studies, serum EBV DNA levels permitted the detection of NHL patients also in immunocompromised hosts [10, 13]. Some of this controversy ...
File
File

... A substance that absorbs light at one wavelength (UV) and re-emits light at a visible wavelength (color) ...
Transcription Regulation Background: Lactose Background: How
Transcription Regulation Background: Lactose Background: How

... • E. coli (we are back with these pesky little coliforms) have the ability to grow in media which contain lactose as their sole carbon source. Lactose is a sugar found in milk (a ...
DNA Lesson 2 Guide
DNA Lesson 2 Guide

... The ribosome uses transfer RNA (tRNA) to read the message and make a protein. This process is called translation. ...
Chapter 11 Protein Characterization
Chapter 11 Protein Characterization

Slides PPT
Slides PPT

... • The lac operon is under two forms of control; positive and negative control. • Negative control occurs when the binding of a protein prevents an event. The brake on the car • Positive control is when the binding causes the event. The accelerator on the ...
Welcome to Our Microbial Genetics Class
Welcome to Our Microbial Genetics Class

... segment two is free to associate with segment three. As a result segment four remains single stranded (figure 12.29c) and cannot serve as a terminator for transcription. It is important to note that the sequence coding for the leader peptide contains two adjacent codons that code for the amino acid ...
Biomolecule Notes
Biomolecule Notes

... Important for growth & reproduction of cells, contains the genetic code (what genes are made from) ...
Document
Document

... cells in a mature tobacco leaf is at least 10 times higher than the average number of leaf cells required to select one chloroplast gene transfer event, which indicates that cells within a single leaf are not genetically identical but may differ in their nuclear genome with respect to the pattern of ...
Note 20 - South Tuen Mun Government Secondary School
Note 20 - South Tuen Mun Government Secondary School

... Discontinuous variation are characters which are not affected by the environment, they are normally controlled by one or a few genes. Their pattern of inheritance can be followed by Mendel's law. (iii) The causes of variation  Independent assortment of chromosomes in meiosis produces a large number ...
Document
Document

... • Spore formation of Streptomyces coelicolor on agar was dependent upon the type of agar used, the inclusion of trace elements, the nitrogen source, and a C/N ration between 40 and 100 (68). ...
Activity #3a - Center for Occupational Research and Development
Activity #3a - Center for Occupational Research and Development

... In Activities #1 and #2, you learned the scientific basis for how DNA microarray technology works and how it can be used to illustrate variations in gene expression by examining the gene expression data from two mythological creatures. Different gene expression results in different characteristics. ...
printer-friendly version of benchmark
printer-friendly version of benchmark

... cause mutations). When changes occur in a somatic (body) cell, only the individual organism is affected. However, if the mutation occurs in a germ (sex) cell, then that change may be passed on to the individual’s offspring. The only genetic information that is passed from one generation to the next ...
Powerpoint
Powerpoint

... The final shape of a protein (its tertiary or quaternary structure) is very specific and enables it to do its job/function. Any change in a proteins’ shape will affect its function. Denaturation is when a protein's tertiary structure is lost. This happens when the bonds between the R groups are bro ...
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Deoxyribozyme



Deoxyribozymes, also called DNA enzymes, DNAzymes, or catalytic DNA, are DNA oligonucleotides that are capable of catalyzing specific chemical reactions, similar to the action of other biological enzymes, such as proteins or ribozymes (enzymes composed of RNA).However, in contrast to the abundance of protein enzymes in biological systems and the discovery of biological ribozymes in the 1980s,there are no known naturally occurring deoxyribozymes.Deoxyribozymes should not be confused with DNA aptamers which are oligonucleotides that selectively bind a target ligand, but do not catalyze a subsequent chemical reaction.With the exception of ribozymes, nucleic acid molecules within cells primarily serve as storage of genetic information due to its ability to form complementary base pairs, which allows for high-fidelity copying and transfer of genetic information. In contrast, nucleic acid molecules are more limited in their catalytic ability, in comparison to protein enzymes, to just three types of interactions: hydrogen bonding, pi stacking, and metal-ion coordination. This is due to the limited number of functional groups of the nucleic acid monomers: while proteins are built from up to twenty different amino acids with various functional groups, nucleic acids are built from just four chemically similar nucleobases. In addition, DNA lacks the 2'-hydroxyl group found in RNA which limits the catalytic competency of deoxyribozymes even in comparison to ribozymes.In addition to the inherent inferiority of DNA catalytic activity, the apparent lack of naturally occurring deoxyribozymes may also be due to the primarily double-stranded conformation of DNA in biological systems which would limit its physical flexibility and ability to form tertiary structures, and so would drastically limit the ability of double-stranded DNA to act as a catalyst; though there are a few known instances of biological single-stranded DNA such as multicopy single-stranded DNA (msDNA), certain viral genomes, and the replication fork formed during DNA replication. Further structural differences between DNA and RNA may also play a role in the lack of biological deoxyribozymes, such as the additional methyl group of the DNA base thymidine compared to the RNA base uracil or the tendency of DNA to adopt the B-form helix while RNA tends to adopt the A-form helix. However, it has also been shown that DNA can form structures that RNA cannot, which suggests that, though there are differences in structures that each can form, neither is inherently more or less catalytic due to their possible structural motifs.
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