The BCM Microarray Core Facility
... Using Illumina GenomeStudio software to analyzer mRNA-seq reads, sequence tables are generated providing information on the position of the sequence within the chromosome. Information on SNPs present in a given sample are also provided in a table describing the position of the SNP, the reference bas ...
... Using Illumina GenomeStudio software to analyzer mRNA-seq reads, sequence tables are generated providing information on the position of the sequence within the chromosome. Information on SNPs present in a given sample are also provided in a table describing the position of the SNP, the reference bas ...
No Origin, No Problem for Yeast DNA Replication
... during each cell cycle. In budding yeast, DNA replication initiates from well-defined origins called autonomously replicating sequences (ARSs), while in multicellular organisms replication it is thought to initiate from broader, less well-defined zones. Interestingly, some recent work has suggested ...
... during each cell cycle. In budding yeast, DNA replication initiates from well-defined origins called autonomously replicating sequences (ARSs), while in multicellular organisms replication it is thought to initiate from broader, less well-defined zones. Interestingly, some recent work has suggested ...
Gene Section ERCC3 (Excision repair cross-complementing 3)
... (NER) and the initiation of RNA polymerase II . Indeed, TFIIH fulfills a dual role in transcription initiation and NER and the role of TFIIH in NER might closely mimic its role in the transcription initiation process. In transcription initiation TFIIH is thought to be involved in unwinding of the pr ...
... (NER) and the initiation of RNA polymerase II . Indeed, TFIIH fulfills a dual role in transcription initiation and NER and the role of TFIIH in NER might closely mimic its role in the transcription initiation process. In transcription initiation TFIIH is thought to be involved in unwinding of the pr ...
Sequencing genomes
... conservation and variation can be identified. • The degree of sequence conservation in the alignment reveals evolutionary relatedness of different sequences • The variation between sequences reflects the changes that have occurred during evolution in the form of substitutions and/or indels. • Identi ...
... conservation and variation can be identified. • The degree of sequence conservation in the alignment reveals evolutionary relatedness of different sequences • The variation between sequences reflects the changes that have occurred during evolution in the form of substitutions and/or indels. • Identi ...
Antiparasitic Agents
... d. Pentamidine (Pentam 300 and Nebupent) i. Binds to the AT regions in DNA and inhibits Type 2 topoisomerase in mitochondria DNA 1. H-bonds to DNA 2. cross links the two strands so that they cannot unwind 3. the amidine group donates reacts with the extra electrons on the nitrogen ii. available IV o ...
... d. Pentamidine (Pentam 300 and Nebupent) i. Binds to the AT regions in DNA and inhibits Type 2 topoisomerase in mitochondria DNA 1. H-bonds to DNA 2. cross links the two strands so that they cannot unwind 3. the amidine group donates reacts with the extra electrons on the nitrogen ii. available IV o ...
what is mutation?
... 4. Sense mutations are the opposite of nonsense mutations. Here, a stop codon is converted into an amino acid codon. Since DNA outside of protein-coding regions contains an average of 3 stop codons per 64, the translation process usually stops after producing a slightly longer protein. – Example: No ...
... 4. Sense mutations are the opposite of nonsense mutations. Here, a stop codon is converted into an amino acid codon. Since DNA outside of protein-coding regions contains an average of 3 stop codons per 64, the translation process usually stops after producing a slightly longer protein. – Example: No ...
PPT File
... DNA methylation in human sperm samples phenotypic differences in the next generation • Sperm samples from oligospermic patients: often contain DNA-methylation defects at imprinted loci ...
... DNA methylation in human sperm samples phenotypic differences in the next generation • Sperm samples from oligospermic patients: often contain DNA-methylation defects at imprinted loci ...
Molecular Genetics
... Formative Test Questions Chapter Assessment Questions Standardized Test Practice ...
... Formative Test Questions Chapter Assessment Questions Standardized Test Practice ...
Replicons
... system could carry little genetic information without L [the mutation rate] becoming unbearably large, and how a primitive system could then improve its fidelity and also evolve into a sexual system with crossover beggars the imagination.” Hoyle F. 1987. "Mathematics of Evolution", Acorn Enterprises ...
... system could carry little genetic information without L [the mutation rate] becoming unbearably large, and how a primitive system could then improve its fidelity and also evolve into a sexual system with crossover beggars the imagination.” Hoyle F. 1987. "Mathematics of Evolution", Acorn Enterprises ...
Synthesis of F-18 Fluoroestradiol (FES)
... strength. It is faster in non-aqueous media but it is not any cleaner. Only one major product is formed, the required FES (1) but an HPLC of the reaction mixture shows an elevated, lumpy baseline suggesting that many minor products are formed. We have hydrolysed the sulfate in 0.1 N HCl at 140ºC in ...
... strength. It is faster in non-aqueous media but it is not any cleaner. Only one major product is formed, the required FES (1) but an HPLC of the reaction mixture shows an elevated, lumpy baseline suggesting that many minor products are formed. We have hydrolysed the sulfate in 0.1 N HCl at 140ºC in ...
Gene regulation I Biochemistry 302
... protease activity of LexA. – LecA inactivates itself by catalyzing its own cleavage at a specific Arg-Gly bond in the middle of the protein. Lehninger Principles of Biochemistry, 4th ed., Ch 28 ...
... protease activity of LexA. – LecA inactivates itself by catalyzing its own cleavage at a specific Arg-Gly bond in the middle of the protein. Lehninger Principles of Biochemistry, 4th ed., Ch 28 ...
ppt - Department of Plant Sciences
... restriction digestion and ligation technology. 3. Describe a novel strategy to generate a T-DNA vector that allows the expression of several genes from a single position in the genome. 4. Discuss the advantages and disadvantages of using plastid vectors for plant transformation and gene expression. ...
... restriction digestion and ligation technology. 3. Describe a novel strategy to generate a T-DNA vector that allows the expression of several genes from a single position in the genome. 4. Discuss the advantages and disadvantages of using plastid vectors for plant transformation and gene expression. ...
DNA Pre-ConceptionStu - the Biology Scholars Program Wiki
... 4. The basic unit or monomer of DNA is composed of a(n): A. Lipid, a molecule of nitrate and one of four amino acids B. Lipid, a molecule of phosphate and one of four amino acids C. Protein, a molecule of nitrate and one of four nitrogen containing bases D. Sugar, a molecule of phosphate and one of ...
... 4. The basic unit or monomer of DNA is composed of a(n): A. Lipid, a molecule of nitrate and one of four amino acids B. Lipid, a molecule of phosphate and one of four amino acids C. Protein, a molecule of nitrate and one of four nitrogen containing bases D. Sugar, a molecule of phosphate and one of ...
lecture presentations
... • Chromosomes contain most of a cell’s genetic material in the form of DNA (deoxyribonucleic acid) • DNA is the substance of genes • Genes are the units of inheritance that transmit information from parents to offspring ...
... • Chromosomes contain most of a cell’s genetic material in the form of DNA (deoxyribonucleic acid) • DNA is the substance of genes • Genes are the units of inheritance that transmit information from parents to offspring ...
1. dia
... Initial velocity should be: P(t) graph must be linear, time has to be short enough. If incubation time ↑, time ↑ for denaturation because of high temperature or pH 2.) pH: optimum depends on the amino acid composition of the enzyme proper ionization is necessary for S – catalytic site interaction; a ...
... Initial velocity should be: P(t) graph must be linear, time has to be short enough. If incubation time ↑, time ↑ for denaturation because of high temperature or pH 2.) pH: optimum depends on the amino acid composition of the enzyme proper ionization is necessary for S – catalytic site interaction; a ...
PDF file - the Houpt Lab
... 1. Uses tRNA to shuttle amino acids to ribosome so there are at least 3 types of RNA in the cell: snRNA, tRNA, and mRNA 2. All proteins start with Methionine because start codon is ATG (although Met can be cut off during protein processing) so could label new proteins with radiolabeled Met 3. Mutati ...
... 1. Uses tRNA to shuttle amino acids to ribosome so there are at least 3 types of RNA in the cell: snRNA, tRNA, and mRNA 2. All proteins start with Methionine because start codon is ATG (although Met can be cut off during protein processing) so could label new proteins with radiolabeled Met 3. Mutati ...
SPLIT RNA Extraction Kit: Pure Fractions for Demanding Applications
... (e.g., by heat denaturation) can severely compromise RNA integrity. Similarly, size-filtration based methods such as gDNA removal columns result in either ineffective gDNA removal or exclusion of longer RNA molecules (Fig. 3). 5 min ...
... (e.g., by heat denaturation) can severely compromise RNA integrity. Similarly, size-filtration based methods such as gDNA removal columns result in either ineffective gDNA removal or exclusion of longer RNA molecules (Fig. 3). 5 min ...
Supplementary Information (doc 45K)
... phenylmethylsulfonyl fluoride, 1 µg/mL aprotinin, and 1 µg/mL pepstatin A), harvested, and treated with SDS lysis buffer for 10 min on ice. The resulting lysates were sonicated to shear the DNA to fragment lengths below 1000 bp (amplitude 60%; 4 x 10 s; Fisher Sonic Dismembrator 60, Pittsburgh, PA). ...
... phenylmethylsulfonyl fluoride, 1 µg/mL aprotinin, and 1 µg/mL pepstatin A), harvested, and treated with SDS lysis buffer for 10 min on ice. The resulting lysates were sonicated to shear the DNA to fragment lengths below 1000 bp (amplitude 60%; 4 x 10 s; Fisher Sonic Dismembrator 60, Pittsburgh, PA). ...
Laboratory of Insect Genetics and Biosciences (IGB) Dept. Biology
... the DNA of mammalian somatic cells”. A recent paper showed the existence of non-CG methylation in mammalian somatic cells (PMID:26030523). In fact, non-CG methylation in mammals seems to be more informative of gene expression than CG methylation. R: It is true that DNA methylation has been detected ...
... the DNA of mammalian somatic cells”. A recent paper showed the existence of non-CG methylation in mammalian somatic cells (PMID:26030523). In fact, non-CG methylation in mammals seems to be more informative of gene expression than CG methylation. R: It is true that DNA methylation has been detected ...
Text S1.
... Lande 1979), but correcting for overlapping generations and the sex-limited expression of laying date. Following Gienapp et al. (2006) we therefore estimated the annual response to selection as: R = VA *β * 0.5 *Prect+1, where Prect+1 is the proportion of adults being first year breeders in year t+1 ...
... Lande 1979), but correcting for overlapping generations and the sex-limited expression of laying date. Following Gienapp et al. (2006) we therefore estimated the annual response to selection as: R = VA *β * 0.5 *Prect+1, where Prect+1 is the proportion of adults being first year breeders in year t+1 ...
C8 Challenge
... repaired by DNA replication. repaired during transcription. repaired during translation. cut out and replaced. ANSWER BACK TO GAME ...
... repaired by DNA replication. repaired during transcription. repaired during translation. cut out and replaced. ANSWER BACK TO GAME ...
No Slide Title
... Eukaryotic cytoplasmic ribosomes are larger and more complex than prokaryotic ribosomes. Mitochondrial and chloroplast ribosomes differ from both examples shown. ...
... Eukaryotic cytoplasmic ribosomes are larger and more complex than prokaryotic ribosomes. Mitochondrial and chloroplast ribosomes differ from both examples shown. ...
E.coli
... Laboratory synthesis of nucleic acids requires complex strategies Functional groups on the monomeric units are reactive and must be blocked Correct phosphodiester linkages must be made Recovery at each step must high! ...
... Laboratory synthesis of nucleic acids requires complex strategies Functional groups on the monomeric units are reactive and must be blocked Correct phosphodiester linkages must be made Recovery at each step must high! ...
Deoxyribozyme
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Deoxyribozymes, also called DNA enzymes, DNAzymes, or catalytic DNA, are DNA oligonucleotides that are capable of catalyzing specific chemical reactions, similar to the action of other biological enzymes, such as proteins or ribozymes (enzymes composed of RNA).However, in contrast to the abundance of protein enzymes in biological systems and the discovery of biological ribozymes in the 1980s,there are no known naturally occurring deoxyribozymes.Deoxyribozymes should not be confused with DNA aptamers which are oligonucleotides that selectively bind a target ligand, but do not catalyze a subsequent chemical reaction.With the exception of ribozymes, nucleic acid molecules within cells primarily serve as storage of genetic information due to its ability to form complementary base pairs, which allows for high-fidelity copying and transfer of genetic information. In contrast, nucleic acid molecules are more limited in their catalytic ability, in comparison to protein enzymes, to just three types of interactions: hydrogen bonding, pi stacking, and metal-ion coordination. This is due to the limited number of functional groups of the nucleic acid monomers: while proteins are built from up to twenty different amino acids with various functional groups, nucleic acids are built from just four chemically similar nucleobases. In addition, DNA lacks the 2'-hydroxyl group found in RNA which limits the catalytic competency of deoxyribozymes even in comparison to ribozymes.In addition to the inherent inferiority of DNA catalytic activity, the apparent lack of naturally occurring deoxyribozymes may also be due to the primarily double-stranded conformation of DNA in biological systems which would limit its physical flexibility and ability to form tertiary structures, and so would drastically limit the ability of double-stranded DNA to act as a catalyst; though there are a few known instances of biological single-stranded DNA such as multicopy single-stranded DNA (msDNA), certain viral genomes, and the replication fork formed during DNA replication. Further structural differences between DNA and RNA may also play a role in the lack of biological deoxyribozymes, such as the additional methyl group of the DNA base thymidine compared to the RNA base uracil or the tendency of DNA to adopt the B-form helix while RNA tends to adopt the A-form helix. However, it has also been shown that DNA can form structures that RNA cannot, which suggests that, though there are differences in structures that each can form, neither is inherently more or less catalytic due to their possible structural motifs.