Predicting Protein Stability Changes upon Mutation Using Database
... core, we estimate the folding free energy changes upon mutation using database-derived potentials and correlate them with the experimentally measured ones. Several potentials are tested, representing different kinds of interactions. Local interactions along the chain are described by torsion potenti ...
... core, we estimate the folding free energy changes upon mutation using database-derived potentials and correlate them with the experimentally measured ones. Several potentials are tested, representing different kinds of interactions. Local interactions along the chain are described by torsion potenti ...
IOSR Journal of Agriculture and Veterinary Science (IOSR-JAVS)
... The highest cumulative average volume of biogas (CAVB) recorded for treatment C 50:50 (cow dung/poultry manure mixture) at the end of 8 weeks of digestion (WOD) agrees with findings of Lehtomakiet al. (32), who reported an optimal yield with mixing ratio 1:1 when cattle manure, grass silage, sugar b ...
... The highest cumulative average volume of biogas (CAVB) recorded for treatment C 50:50 (cow dung/poultry manure mixture) at the end of 8 weeks of digestion (WOD) agrees with findings of Lehtomakiet al. (32), who reported an optimal yield with mixing ratio 1:1 when cattle manure, grass silage, sugar b ...
15Nitrogen metabolism
... - Many extrahepatic tissues (brain ) produce NH4+ from metabolic processes as nucleotide degradation. -This toxic ammonia is converted into amino group of glutamine that transported to liver or kidneys. - Glutamine: non-toxic transport form of NH4+ and also source of amino group in many biosynthesis ...
... - Many extrahepatic tissues (brain ) produce NH4+ from metabolic processes as nucleotide degradation. -This toxic ammonia is converted into amino group of glutamine that transported to liver or kidneys. - Glutamine: non-toxic transport form of NH4+ and also source of amino group in many biosynthesis ...
Bioenergetics and ioenergetics and Metabolism etabolism
... (c) to assemble these precursors into proteins, lipids, polysaccharides, nucleic acids and other cell components. (d) to form and degrade biomolecules required in specialized functions of cells. These features of metabolism are closely inter related since the synthesis of the molecules, that are a c ...
... (c) to assemble these precursors into proteins, lipids, polysaccharides, nucleic acids and other cell components. (d) to form and degrade biomolecules required in specialized functions of cells. These features of metabolism are closely inter related since the synthesis of the molecules, that are a c ...
representation and display of non-standard peptides using semi
... • The solution to this problem is the systematic naming of amino acid derivatives. • This approach is well known to peptide chemists, with these names routinely used in scientific articles, patent applications and supplier catalogues, and easily recognizable by chemists. • Alas this “de facto” nomen ...
... • The solution to this problem is the systematic naming of amino acid derivatives. • This approach is well known to peptide chemists, with these names routinely used in scientific articles, patent applications and supplier catalogues, and easily recognizable by chemists. • Alas this “de facto” nomen ...
Two Microtubule-Associated Proteins of the
... To obtain proteins for in vitro analysis, cDNA sequences of AtMAP65-1 and AtMAP65-6 were obtained in a cDNA clone (109M12) and by reverse transcription (RT)-PCR, respectively. Purified glutathione S-transferase (GST)-AtMAP65-1 fusion protein appeared in doublets (Fig. 1A). GST-AtMAP65-6 appeared in ...
... To obtain proteins for in vitro analysis, cDNA sequences of AtMAP65-1 and AtMAP65-6 were obtained in a cDNA clone (109M12) and by reverse transcription (RT)-PCR, respectively. Purified glutathione S-transferase (GST)-AtMAP65-1 fusion protein appeared in doublets (Fig. 1A). GST-AtMAP65-6 appeared in ...
Modifying the chain-length selectivity of the
... 2000). In the case of BCL, it contains a binding pocket like an elliptical funnel with a length of 17 Å. The width at its base is 4.5 Å and increases to 10.5 Å at the entrance to the binding site. The left- and the right-hand walls viewed along the alcohol–acid axis are 10.5 and 16.5 Å, respectively ...
... 2000). In the case of BCL, it contains a binding pocket like an elliptical funnel with a length of 17 Å. The width at its base is 4.5 Å and increases to 10.5 Å at the entrance to the binding site. The left- and the right-hand walls viewed along the alcohol–acid axis are 10.5 and 16.5 Å, respectively ...
The Protein Data Bank (PDB) & PDB Files
... • Text file, viewed/modified in editor (TextPad) • a.k.a. structure file • Contains position of every atom in the file as well as some information about the structure • Holds all the information needed to reconstruct a 3D model of a molecule – view in RASMOL or DeepView • At first, contents are inti ...
... • Text file, viewed/modified in editor (TextPad) • a.k.a. structure file • Contains position of every atom in the file as well as some information about the structure • Holds all the information needed to reconstruct a 3D model of a molecule – view in RASMOL or DeepView • At first, contents are inti ...
MS Word Version - Interactive Physiology
... • Now let's see what happens when base is added to the protein. • When there is excess base in the body, proteins release hydrogen ion from side chains that are weak acids. • Notice that the shape of the protein did not change much here because only small amounts of acid or base were added. If the p ...
... • Now let's see what happens when base is added to the protein. • When there is excess base in the body, proteins release hydrogen ion from side chains that are weak acids. • Notice that the shape of the protein did not change much here because only small amounts of acid or base were added. If the p ...
MS Word Version
... • Now let's see what happens when base is added to the protein. • When there is excess base in the body, proteins release hydrogen ion from side chains that are weak acids. • Notice that the shape of the protein did not change much here because only small amounts of acid or base were added. If the p ...
... • Now let's see what happens when base is added to the protein. • When there is excess base in the body, proteins release hydrogen ion from side chains that are weak acids. • Notice that the shape of the protein did not change much here because only small amounts of acid or base were added. If the p ...
Document
... • Human formyl peptide receptor like-1 (FPRL-1) receptor expressed in yeast • Expression of random peptides (13-mers) linked to secretion signal • Activation of receptor-coupled pathway linked to expression of HIS3 reporter • Three rounds of selection yielded 5 positive peptides out of 106 initial c ...
... • Human formyl peptide receptor like-1 (FPRL-1) receptor expressed in yeast • Expression of random peptides (13-mers) linked to secretion signal • Activation of receptor-coupled pathway linked to expression of HIS3 reporter • Three rounds of selection yielded 5 positive peptides out of 106 initial c ...
Structural and Functional Studies on Proteinaceous Metallocarboxypeptidase Inhibitors Joan López Arolas
... 2003; Kuzuya & Iguchi, 2003). Besides the physiological necessity, many of these peptidases are potentially hazardous to their natural environment therefore their proteolytic activity must be carefully controlled by the cell/organism. This control is normally achieved by regulated expression/secreti ...
... 2003; Kuzuya & Iguchi, 2003). Besides the physiological necessity, many of these peptidases are potentially hazardous to their natural environment therefore their proteolytic activity must be carefully controlled by the cell/organism. This control is normally achieved by regulated expression/secreti ...
galactose specific
... Legume lectins: peanut lectin, winged bean basic and acidic lectins and their complexes • Multimeric proteins can assume open quaternary structures • High variability in quaternary structure while the tertiary structure is essentially same • Water bridges as a strategy for generating ligand specifi ...
... Legume lectins: peanut lectin, winged bean basic and acidic lectins and their complexes • Multimeric proteins can assume open quaternary structures • High variability in quaternary structure while the tertiary structure is essentially same • Water bridges as a strategy for generating ligand specifi ...
Active Site of Ribonuclease A - Department of Biochemistry | UW
... tenfold (D121A). The alterations do not perturb the three-dimensional structure of the crystalline enzyme, and do not alter the pH-rate profile for hydrolysis. These effects are far less than those observed for analogous variants of serine proteases (Craik et al. 1987; Sprang et al. 1987; Carter and ...
... tenfold (D121A). The alterations do not perturb the three-dimensional structure of the crystalline enzyme, and do not alter the pH-rate profile for hydrolysis. These effects are far less than those observed for analogous variants of serine proteases (Craik et al. 1987; Sprang et al. 1987; Carter and ...
Purification and Properties of the Dictyostelium Calpain
... at 77 kDa, which comigrated with the Cpl immunoreactivity (Figure 4B). Trace amounts of 50 kDa protein were also present in this preparation. In several other preparations of Cpl, both 60 and 50 kDa bands were observed. Autoproteolysis of Cpl. Phenyl-Sepharose-purified Cpl had no detectable caseinol ...
... at 77 kDa, which comigrated with the Cpl immunoreactivity (Figure 4B). Trace amounts of 50 kDa protein were also present in this preparation. In several other preparations of Cpl, both 60 and 50 kDa bands were observed. Autoproteolysis of Cpl. Phenyl-Sepharose-purified Cpl had no detectable caseinol ...
enzymes - Yengage
... enzyme activity Velocity V of an enzyme-catalyzed reaction is measured at various pH values and plotted ,a bell-shaped curve is obtained Enzyme have an optimum pH on both sides of which the velocity will be drastically reduced pH changes will cause alteration in charged state of enzyme /substrate ...
... enzyme activity Velocity V of an enzyme-catalyzed reaction is measured at various pH values and plotted ,a bell-shaped curve is obtained Enzyme have an optimum pH on both sides of which the velocity will be drastically reduced pH changes will cause alteration in charged state of enzyme /substrate ...
Highly Conserved Region 141–168 of the NS1 Protein Is a New
... 3F10, 4E5, 5E2, 5G12, 2H11, and 3C4) reacted with DENV2. To identify the epitope regions of these MAbs, a series of recombinant DENV-2 NS1 truncated mutants was expressed in 293T cells. The plasma from DENV-infected patients reacted with all mutant NS1s except one (313–352), thus indicating that B-c ...
... 3F10, 4E5, 5E2, 5G12, 2H11, and 3C4) reacted with DENV2. To identify the epitope regions of these MAbs, a series of recombinant DENV-2 NS1 truncated mutants was expressed in 293T cells. The plasma from DENV-infected patients reacted with all mutant NS1s except one (313–352), thus indicating that B-c ...
Modelling of Protein Breakdown During Critical Illness
... Substrate(carbohydrate, fat) −−−−−−→ CO2 + H2 O + energy ...
... Substrate(carbohydrate, fat) −−−−−−→ CO2 + H2 O + energy ...
Nutrition, Metabolism, and Body Temperature Regulation
... Proteins are important structural materials of the body, including, for example, keratin in skin, collagen and elastin in connective tissues, and muscle proteins. In addition, functional proteins such as enzymes and some hormones regulate an incredible variety of body functions. Whether amino acids ...
... Proteins are important structural materials of the body, including, for example, keratin in skin, collagen and elastin in connective tissues, and muscle proteins. In addition, functional proteins such as enzymes and some hormones regulate an incredible variety of body functions. Whether amino acids ...
Modulation of functional properties of bifunctional S- Adenosylmethionine decarboxylase/Ornithine decarboxylase of
... Trishydroxy (methyl-amino) methane / Hydrochloric acid ...
... Trishydroxy (methyl-amino) methane / Hydrochloric acid ...
Import of Agrobacterium T-DNA into Plant Nuclei: Two
... To study the mechanism of nuclear import of T-DNA, complexes consisting of the virulence proteins VirD2 and VirE2 as well as single-stranded DNA (ssDNA) were tested for import into plant nuclei in vitro. Import of these complexes was fast and efficient and could be inhibited by a competitor, a nucle ...
... To study the mechanism of nuclear import of T-DNA, complexes consisting of the virulence proteins VirD2 and VirE2 as well as single-stranded DNA (ssDNA) were tested for import into plant nuclei in vitro. Import of these complexes was fast and efficient and could be inhibited by a competitor, a nucle ...
Characterization and the role of carbonic anhydrase
... In cyanobacteria, for the carbon concentrating mechanism to function, there is an absolute requirement for carbonic anhydrase activity within the carboxysome to convert the bicarbonate to carbon dioxide, and a simultaneous requirement that minimal carbonic anhydrase activity be found within the cyst ...
... In cyanobacteria, for the carbon concentrating mechanism to function, there is an absolute requirement for carbonic anhydrase activity within the carboxysome to convert the bicarbonate to carbon dioxide, and a simultaneous requirement that minimal carbonic anhydrase activity be found within the cyst ...
The biosynthesis of peptidoglycan lipid
... & Strominger (1972) about 30 years ago and some of its properties were investigated. Its optimal pH for activity was near 7.5, the enzyme did not require any cation, was stimulated by nonionic Triton detergents, and failed to hydrolyze isopentenyl pyrophosphate. The gene for this activity, however, ...
... & Strominger (1972) about 30 years ago and some of its properties were investigated. Its optimal pH for activity was near 7.5, the enzyme did not require any cation, was stimulated by nonionic Triton detergents, and failed to hydrolyze isopentenyl pyrophosphate. The gene for this activity, however, ...
Proteolysis
Proteolysis is the breakdown of proteins into smaller polypeptides or amino acids. Uncatalysed, the hydrolysis of peptide bonds is extremely slow, taking hundreds of years. Proteolysis is typically catalysed by cellular enzymes called proteases, but may also occur by intra-molecular digestion. Low pH or high temperatures can also cause proteolysis non-enzymatically.Proteolysis in organisms serves many purposes; for example, digestive enzymes break down proteins in food to provide amino acids for the organism, while proteolytic processing of a polypeptide chain after its synthesis may be necessary for the production of an active protein. It is also important in the regulation of some physiological and cellular processes, as well as preventing the accumulation of unwanted or abnormal proteins in cells. Consequently, dis-regulation of proteolysis can cause diseases, and is used in some venoms to damage their prey.Proteolysis is important as an analytical tool for studying proteins in the laboratory, as well as industrially, for example in food processing and stain removal.