Arg305 of Streptomyces l-glutamate oxidase plays a crucial role for
... formed between the side chain of the substrate L-arginine and that of the substituted residue. In fact, only R305D LGOX had the catalytic activity toward L-arginine among the mutant enzymes (Fig. 2 and Table 1). It is thought that activity with L-arginine is enhanced by a suitable distance because o ...
... formed between the side chain of the substrate L-arginine and that of the substituted residue. In fact, only R305D LGOX had the catalytic activity toward L-arginine among the mutant enzymes (Fig. 2 and Table 1). It is thought that activity with L-arginine is enhanced by a suitable distance because o ...
View - BioOne
... Jianying Shen, Antonio DiTommaso, Mingquan Shen, Wei Lu, and Zhengming Li* Nitrogen-fixing cyanobacteria are vital photosynthetic microorganisms that contribute to soil fertility by fixing atmospheric nitrogen and are also important for maintaining ecosystem stability. These microorganisms can be ve ...
... Jianying Shen, Antonio DiTommaso, Mingquan Shen, Wei Lu, and Zhengming Li* Nitrogen-fixing cyanobacteria are vital photosynthetic microorganisms that contribute to soil fertility by fixing atmospheric nitrogen and are also important for maintaining ecosystem stability. These microorganisms can be ve ...
Scrutinizing GMO Risk Assessment Evaluating the practice of risk
... Recombinant DNA (stability, potential for gene transfer) Protein Expression Product of the novel DNA (effects on function; potential toxicity; potential allergenicity) Possible Secondary Effects from gene expression or disruption of host DNA or metabolic pathways, including composition of critical m ...
... Recombinant DNA (stability, potential for gene transfer) Protein Expression Product of the novel DNA (effects on function; potential toxicity; potential allergenicity) Possible Secondary Effects from gene expression or disruption of host DNA or metabolic pathways, including composition of critical m ...
BIOGENESIS OF MITOCHONDRIAL ATPase Studies on
... binding of F 1 to mitochondrial membrane and thus is one of the components which are essential for cold-stable and oligomycin-sensitive ATPase. The activity of OSCP increases during glucose derepression two to three fold as shown by reconstitution experiments with OSCP-enriched fractions [11]. The e ...
... binding of F 1 to mitochondrial membrane and thus is one of the components which are essential for cold-stable and oligomycin-sensitive ATPase. The activity of OSCP increases during glucose derepression two to three fold as shown by reconstitution experiments with OSCP-enriched fractions [11]. The e ...
Full-Text PDF
... pathological conditions. For example, the increased vascular constriction observed after PGF2α treatment of hyperplastic rabbit carotid artery correlated with higher levels of diphosphorylated LC20 [15]. Cerebral vasospasm is a serious, often fatal, complication of subarachnoid hemorrhage, and durin ...
... pathological conditions. For example, the increased vascular constriction observed after PGF2α treatment of hyperplastic rabbit carotid artery correlated with higher levels of diphosphorylated LC20 [15]. Cerebral vasospasm is a serious, often fatal, complication of subarachnoid hemorrhage, and durin ...
... type collagens, in which consisted of α- and β-chains as major components. PSC was rich in glycine and had high content of imino acids (196 residues/1000 residues). The maximum transition temperature (Tm) of PSC was 34 °C which was about 7 °C lower than that of CC. The skin waste material from cuttl ...
Two Arabidopsis Genes (IPMS1 and IPMS2
... an active IPMS because such an enzyme activity is absolutely essential to the plant for its synthesis of Leu. In this study, IPMS1 and IPMS2 were cloned and heterologously expressed in E. coli. Determination of the substrate specificity of the purified proteins in conjunction with analysis of knocko ...
... an active IPMS because such an enzyme activity is absolutely essential to the plant for its synthesis of Leu. In this study, IPMS1 and IPMS2 were cloned and heterologously expressed in E. coli. Determination of the substrate specificity of the purified proteins in conjunction with analysis of knocko ...
The role of AMP-activated protein kinase in the coordination of
... mTOR promotes its dissociation from eIF4E bound to the mRNA 7-methylguanosine cap structure, allowing the assembly of the preinitiation complex (128). S6K1 activation needs initial phosphorylation by mTORC1 at Thr389 (101) and additional inputs on Thr229 for full activation by the phosphoinositide-d ...
... mTOR promotes its dissociation from eIF4E bound to the mRNA 7-methylguanosine cap structure, allowing the assembly of the preinitiation complex (128). S6K1 activation needs initial phosphorylation by mTORC1 at Thr389 (101) and additional inputs on Thr229 for full activation by the phosphoinositide-d ...
Regulation of Translation by Essential Amino Acids and Glucose in
... To everyone who has contributed to this chapter of my life - please consider this is the ultimate thank you, as none of it would have happened without the brilliance, support, and good humor of each one of you. Most significantly, to my advisor Dr. John Cant – thank you for providing me with every o ...
... To everyone who has contributed to this chapter of my life - please consider this is the ultimate thank you, as none of it would have happened without the brilliance, support, and good humor of each one of you. Most significantly, to my advisor Dr. John Cant – thank you for providing me with every o ...
Biochemistry Ch 33 597-624 [4-20
... -because adipose lacks glycerol kinase and cannot use glycerol produced by LPL, glycerol travels back to liver, which it uses to synthesize more triacylglycerol; in adipose, glycerol-3-phosphate is from glucose -in addition to stimulating synthesis and release of LPL, insulin stimulates glucose meta ...
... -because adipose lacks glycerol kinase and cannot use glycerol produced by LPL, glycerol travels back to liver, which it uses to synthesize more triacylglycerol; in adipose, glycerol-3-phosphate is from glucose -in addition to stimulating synthesis and release of LPL, insulin stimulates glucose meta ...
Reading - Chapter 15 and the Appendix to Chapter 15
... The differences in O2 affinity between T-State (deoxy) and R-State (oxy) Hb can be understood in terms of the changes in quaternary structure that accompany the conversion of deoxy Hb to oxy Hb. o The shift from the deoxy to oxy conformation arises from the fact that in deoxy Hb the iron lies out of ...
... The differences in O2 affinity between T-State (deoxy) and R-State (oxy) Hb can be understood in terms of the changes in quaternary structure that accompany the conversion of deoxy Hb to oxy Hb. o The shift from the deoxy to oxy conformation arises from the fact that in deoxy Hb the iron lies out of ...
Safety assessment - Federal Register of Legislation
... Monsanto Australia Limited is seeking approval in the Australia New Zealand Food Standards Code (the Code) for food derived from corn line 88017 (MON 88017), a variety that has been genetically modified (GM) for insect-protection and herbicide-tolerance. Standard 1.5.2 – Food Produced Using Gene Tec ...
... Monsanto Australia Limited is seeking approval in the Australia New Zealand Food Standards Code (the Code) for food derived from corn line 88017 (MON 88017), a variety that has been genetically modified (GM) for insect-protection and herbicide-tolerance. Standard 1.5.2 – Food Produced Using Gene Tec ...
Metabolic changes in Diabetes mellitus (DM)
... sensitive cells. Intermediate (minutes): • Stimulation of protein synthesis • Inhibition of protein degradation • Activation of glycogen synthase and increased glycogenesis • Inhibition of phosphorylase and gluconeogenic enzymes (decreased glycogenolysis and gluconeogenesis) Delayed actions (hours): ...
... sensitive cells. Intermediate (minutes): • Stimulation of protein synthesis • Inhibition of protein degradation • Activation of glycogen synthase and increased glycogenesis • Inhibition of phosphorylase and gluconeogenic enzymes (decreased glycogenolysis and gluconeogenesis) Delayed actions (hours): ...
lec-08-handout
... Trypsin- it is highly specific, it cleaves peptide on carboxyl side of Lys/Arg. Another enzyme thrombin, which participates in blood clotting, it is even more specific that trypsin. It catalyses hydrolysis of Arg/Gly bonds in a very specific peptide sequence and therefore offers high specificity. ...
... Trypsin- it is highly specific, it cleaves peptide on carboxyl side of Lys/Arg. Another enzyme thrombin, which participates in blood clotting, it is even more specific that trypsin. It catalyses hydrolysis of Arg/Gly bonds in a very specific peptide sequence and therefore offers high specificity. ...
Database search and pairwise alignments
... PAM matrices 12 High order PAM matrices are generated by successive multiplications of the PAM 1 matrix, since the probability of two independent events is equal to the product of the probabilities of each individual event While for the PAM 1 matrix it holds that a mutational event corresponds to ...
... PAM matrices 12 High order PAM matrices are generated by successive multiplications of the PAM 1 matrix, since the probability of two independent events is equal to the product of the probabilities of each individual event While for the PAM 1 matrix it holds that a mutational event corresponds to ...
Coevolution of an aminoacyl-tRNA synthetase with its tRNA substrates
... D-GluRS expanded its tRNA specificity to the recognition of tRNAGlu with a glutamine-specific anticodon (14). However, this work did not afford real insight into a ND-GluRS, because the tRNA specificity was not changed. In addition to the lack of some synthetases (see above), whole genome analysis a ...
... D-GluRS expanded its tRNA specificity to the recognition of tRNAGlu with a glutamine-specific anticodon (14). However, this work did not afford real insight into a ND-GluRS, because the tRNA specificity was not changed. In addition to the lack of some synthetases (see above), whole genome analysis a ...
439EnPanc13
... Exocrine portion: secretes digestive enzymes via pancreatic duct, to small intestine. Endocrine portion: pancreatic islets (of Langerhans), involved in regulation of blood glucose levels. ...
... Exocrine portion: secretes digestive enzymes via pancreatic duct, to small intestine. Endocrine portion: pancreatic islets (of Langerhans), involved in regulation of blood glucose levels. ...
Enzymes
... called trypsin. Most other enzymes are named for their substrates and for the reactions that they catalyze, with the suffix "ase" added. Thus, an ATPase is an enzyme that breaks down ATP,whereas ATP synthase is an enzyme that synthesizes ...
... called trypsin. Most other enzymes are named for their substrates and for the reactions that they catalyze, with the suffix "ase" added. Thus, an ATPase is an enzyme that breaks down ATP,whereas ATP synthase is an enzyme that synthesizes ...
Interspecific and intraspecific composition and variation of free
... found no differences between algae in relation to their combined amino acids compositions. Similar conclusions were reached earlier by Parsons et al. (1961). The major amino acids measured in the combined fraction were LEU, ALA, ASP, GLU, GLY, LYS and ARG. Ennght et al. (1986) also noticed that the ...
... found no differences between algae in relation to their combined amino acids compositions. Similar conclusions were reached earlier by Parsons et al. (1961). The major amino acids measured in the combined fraction were LEU, ALA, ASP, GLU, GLY, LYS and ARG. Ennght et al. (1986) also noticed that the ...
PDF - Biochemical Society Transactions
... The formation of 1,3-dipoles from imines of both uamino acids and a-amino acid esters has relevance, we believe, to the biochemistry of pyridoxal. The pyridoxal imines of a-amino acids function, in the case of racemases and transaminases, by removal of the proton u to the carboxyl group and protonat ...
... The formation of 1,3-dipoles from imines of both uamino acids and a-amino acid esters has relevance, we believe, to the biochemistry of pyridoxal. The pyridoxal imines of a-amino acids function, in the case of racemases and transaminases, by removal of the proton u to the carboxyl group and protonat ...
Cell and Molecular Biology
... You have isolated an antibiotic named edeine, from a bacterial culture. Edeine inhibits protein synthesis but has not effect on either DNA synthesis or RNA synthesis. When added to a reticulocyte lysate, edeine stops protein synthesis after a short lag, as shown below. By contrast, cycloheximide st ...
... You have isolated an antibiotic named edeine, from a bacterial culture. Edeine inhibits protein synthesis but has not effect on either DNA synthesis or RNA synthesis. When added to a reticulocyte lysate, edeine stops protein synthesis after a short lag, as shown below. By contrast, cycloheximide st ...
Proteolysis
Proteolysis is the breakdown of proteins into smaller polypeptides or amino acids. Uncatalysed, the hydrolysis of peptide bonds is extremely slow, taking hundreds of years. Proteolysis is typically catalysed by cellular enzymes called proteases, but may also occur by intra-molecular digestion. Low pH or high temperatures can also cause proteolysis non-enzymatically.Proteolysis in organisms serves many purposes; for example, digestive enzymes break down proteins in food to provide amino acids for the organism, while proteolytic processing of a polypeptide chain after its synthesis may be necessary for the production of an active protein. It is also important in the regulation of some physiological and cellular processes, as well as preventing the accumulation of unwanted or abnormal proteins in cells. Consequently, dis-regulation of proteolysis can cause diseases, and is used in some venoms to damage their prey.Proteolysis is important as an analytical tool for studying proteins in the laboratory, as well as industrially, for example in food processing and stain removal.