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Biology / Biochemistry News
Biology / Biochemistry News

... that there are enzymes that are removing NO from proteins to control protein activity," Stamler said. "This action has a broad-based effect, frankly, and probably happens in virtually all cells and across all protein classes. Nitric oxide is implicated in many disease processes. Sepsis, asthma, cyst ...
File - Mrs. Pisciotta`s Biology Classes
File - Mrs. Pisciotta`s Biology Classes

... such as olive oil, tend to be liquid at room temperature. Other cooking oils, such as corn oil, sesame oil, canola oil, and peanut oil, contain polyunsaturated lipids. ...
2-3
2-3

... such as olive oil, tend to be liquid at room temperature. Other cooking oils, such as corn oil, sesame oil, canola oil, and peanut oil, contain polyunsaturated lipids. ...
The Chemistry of Burgers
The Chemistry of Burgers

... browning. In fact, the Maillard reaction is also called the browning reaction. Maillard found that when he heated amino acids, remember the beads in the protein necklaces? Plus sugars like those in carbohydrates, which are in almost all natural foods, including meat, the substance turned brown, diff ...
Molecular cloning, expression, and bioactivity of dove B lymphocyte
Molecular cloning, expression, and bioactivity of dove B lymphocyte

... transmembrane domain and a putative furin protease cleavage site like cBAFF, hBAFF and mBAFF. Amino acids sequence comparison revealed that the doBAFF has a very high sequence and structural similarity to other avian BAFFs. From the phylogenetic analysis of BAFF, we detected the genetic relationship ...
Nuclear Transport of Plant Potyviral Proteins
Nuclear Transport of Plant Potyviral Proteins

... INTRODUCTION Many proteins undergo post-translational modifications and are targeted to different subcellular compartments. Transit into the secretory pathway (Klausner, 1989) and into some organelles, such as chloroplasts (Ellis, 1981) and mitochondria (Wickner and Lodish, 1985), is mediated by a s ...
AIBSTCT Nucleic Acids Research - Walter Lab
AIBSTCT Nucleic Acids Research - Walter Lab

... containing only the N-terminal 68 amino acids (SRP19N, lanes 7-11). In vitro translation products were synthesized in the absence (lane 1) or in the presence (lanes 2 and 7) of added SRP19 mRNA. Lanes 3-4 and 8-9: products immunoprecipitated with rabbit anti SRP19 antiserum under native (lanes 3 and ...
Genetically engineered single-chain antibody fusion proteins
Genetically engineered single-chain antibody fusion proteins

... The genetic approach makes possible the improvement of the antibody affinity by genetic engineering in order to reach or exceed the sensitivity level. ...
Document
Document

... • Enzymes are a type of protein that acts as a catalyst to speed up chemical reactions • Enzymes can perform their functions repeatedly, functioning as workhorses that carry out the processes of life ...
sorghum protein - Simply Sorghum
sorghum protein - Simply Sorghum

... bran is a rich source of condensed tannins, also known as proanthocyanidins, specifically containing epicatechin and catechin. Black sorghum bran contains anthocyanins, including 3-deoxyanthocyanins (apigeninidin) and luteolinidin7. The high levels of 3-deoxyanthocyanins are relatively rare in natur ...
Metabolic Processes
Metabolic Processes

... • The solubility of many molecules is determined by their electronegativity and molecular structure. • Sugars, such as glucose, have many hydroxyl (OH) groups, which tend to increase the solubility of the molecule. • Molecules that lack polar covalent bonds, such as lipids, clump together in water b ...
Principles of Protein Structure
Principles of Protein Structure

... • The chemical nature of the carboxyl and amino groups of all amino acids permit hydrogen bond formation (stability) and hence defines secondary structures within the protein. • The R group has an impact on the likelihood of secondary structure formation (proline is an extreme case) • This leads to ...
IN VITRO TRANSCRIPTION . TRANSLATION - UTH e
IN VITRO TRANSCRIPTION . TRANSLATION - UTH e

... identified. In comparison to eukaryotic systems, the E.coli extract has a relatively simple translational apparatus with less complicated control at the initiation level, allowing this system to be very efficient in protein synthesis. Bacterial extracts are often unsuitable for translation of RNA, b ...
Targeting to the T. gondii plastid
Targeting to the T. gondii plastid

... 3970 A. DeRocher and others is mediated by an N-terminal bipartite targeting sequence composed of an ER signal sequence followed by a chloroplast transit peptide-like domain (Schwartzbach et al., 1998). Like the chloroplasts of diatoms and euglenoids, the T. gondii apicoplast appears to have arisen ...
Defining the inner membrane proteome of E coli
Defining the inner membrane proteome of E coli

... • We have estimated the overexpression potential and suggest that a large fraction can be produced in sufficient quantities for biochemical and structural work • The final constrained topology models are now deposited in the uniprot database ...
Hein and Arena
Hein and Arena

... compounds such as nucleic acids, or 3. Deaminated to a keto acid, which can either be used to synthesize other compounds or be oxidized to carbon dioxide and water to provide energy. ...
Protein Synthesis
Protein Synthesis

... by a protein and lipid membrane envelope. The genetic material is RNA. HIV also carries two molecules of the enzyme reverse transcriptase, ready to copy the RNA after entry into a host cell. ...
what we`re reading
what we`re reading

... 1. Protein Concentrate vs. Isolate: Protein is derived from various food sources and is “concentrated” by removing the non-protein parts. The result: a powder that’s 70 to 85 percent pure protein (with the remaining 15 to 30 percent consisting mostly of carbohydrates and fat). Taking the concentrat ...
Signal Transduction From the Endoplasmic Reticulum to the Cell
Signal Transduction From the Endoplasmic Reticulum to the Cell



... correct order of the CNBr fragments, e.g. 1-2-3. Justify your approach on the back of the previous page. 1 – 3 – 2. (4 pts for correct answer) CNBr 1 is the first fragment since its sequence agrees with the amino-terminal sequence of the peptide. The third CNBr fragment has an Arginine, which is a c ...
Molecules of the Cell: The Building Blocks of Life
Molecules of the Cell: The Building Blocks of Life

... Polysaccharides The polysaccharides are extremely large and complex carbohydrate molecules. A ­single polysaccharide molecule may contain hundreds or thousands of monosaccharide subunits bonded together through dehydration synthesis reactions. One example of an “energy polysaccharide” is starch, whi ...
Chapter 16
Chapter 16

... • Lysozyme hydrolyzes polysaccharide chains and ruptures certain bacterial cells by breaking down the cell wall. • Hydrolyzes at glycosidic bond of Nacetylmuramic acid residue. (See Figure 16.31) ...
Chapter 3: The Chemical Basis for Life Lesson 2: Organic Compounds
Chapter 3: The Chemical Basis for Life Lesson 2: Organic Compounds

Transition
Transition

... Properties of Serine Proteases Zymogens Are Inactive Enzyme Precursors • Digestive serine proteases including trypsin, chymotrypsin, and elastase are synthesized and stored in the pancreas as zymogens • Storage of hydrolytic enzymes as zymogens prevents damage to cell proteins • Zymogens are activ ...
Synaptonemal complex proteins - Journal of Cell Science
Synaptonemal complex proteins - Journal of Cell Science

... screened by affinity elution (Sambrook et al., 1989). The eluted antibodies were immune tested on spermatocytes in order to detect those phage clones that have a cDNA insert that produces meiotic chromosome antigens. The inserts of choice were excised in vivo as Bluescript plasmids following the man ...
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Proteolysis



Proteolysis is the breakdown of proteins into smaller polypeptides or amino acids. Uncatalysed, the hydrolysis of peptide bonds is extremely slow, taking hundreds of years. Proteolysis is typically catalysed by cellular enzymes called proteases, but may also occur by intra-molecular digestion. Low pH or high temperatures can also cause proteolysis non-enzymatically.Proteolysis in organisms serves many purposes; for example, digestive enzymes break down proteins in food to provide amino acids for the organism, while proteolytic processing of a polypeptide chain after its synthesis may be necessary for the production of an active protein. It is also important in the regulation of some physiological and cellular processes, as well as preventing the accumulation of unwanted or abnormal proteins in cells. Consequently, dis-regulation of proteolysis can cause diseases, and is used in some venoms to damage their prey.Proteolysis is important as an analytical tool for studying proteins in the laboratory, as well as industrially, for example in food processing and stain removal.
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