Identification and Characterization of the Potato Leafroll Virus
... Within one of the cDNA clones an open reading frame (ORF) encoding a 23K protein was identified and further characterized. Amino acid sequence comparison of this protein showed significant homology (47.1~) with the barley yellow dwarf virus (BYDV-PAV) coat protein. This and other observations sugges ...
... Within one of the cDNA clones an open reading frame (ORF) encoding a 23K protein was identified and further characterized. Amino acid sequence comparison of this protein showed significant homology (47.1~) with the barley yellow dwarf virus (BYDV-PAV) coat protein. This and other observations sugges ...
Hepatitis C virus (HCV) - HAL
... difficulties in interpreting SDS-PAGE results for HCV mutant core proteins, and highlight the need for comparisons with relevant reference peptides. It should be also noted that the interaction of the HCV mutant core proteins with lipid droplets was not investigated in most of these previous studies ...
... difficulties in interpreting SDS-PAGE results for HCV mutant core proteins, and highlight the need for comparisons with relevant reference peptides. It should be also noted that the interaction of the HCV mutant core proteins with lipid droplets was not investigated in most of these previous studies ...
The Case Against a Darwinian Origin of Protein Folds
... chances of confusing any one amino acid for any other during protein synthesis, and (as already noted) we see that the products of this tightly controlled process are long proteins. Lastly, we see that these long proteins perform an impressive variety of functions with equally impressive specificity ...
... chances of confusing any one amino acid for any other during protein synthesis, and (as already noted) we see that the products of this tightly controlled process are long proteins. Lastly, we see that these long proteins perform an impressive variety of functions with equally impressive specificity ...
bioknowledgy study guide
... 2.1.U4 Metabolism is the web of all the enzyme-catalysed reactions in a cell or organism. 1. The structure of DNA was discovered in 1953, since then molecular Biology has transformed our understanding of living processes. a. Outline the relationship between genes (DNA) and polypeptides. ...
... 2.1.U4 Metabolism is the web of all the enzyme-catalysed reactions in a cell or organism. 1. The structure of DNA was discovered in 1953, since then molecular Biology has transformed our understanding of living processes. a. Outline the relationship between genes (DNA) and polypeptides. ...
Circadian Rhythm of Intestinal SucraseActivity in Rats
... of 0.5 mg/ml after 60 min of incubation with papain and its inclusion during immunoprecipitation did not affect the pattern found on sodium dodecyl sulfate-acrylamide gel electrophoresis of immunoprecipitates, so that a protease inhibitor was not used.) Resultant supernatant fractions were dialyzed ...
... of 0.5 mg/ml after 60 min of incubation with papain and its inclusion during immunoprecipitation did not affect the pattern found on sodium dodecyl sulfate-acrylamide gel electrophoresis of immunoprecipitates, so that a protease inhibitor was not used.) Resultant supernatant fractions were dialyzed ...
Biosynthesis of amino acids
... 1. All the amino nitrogen from amino acids that undergo transamination can be concentrated in glutamate. 2. Release of nitrogen as ammonia is catalyzed by hepatic Lglutamate dehydrogenase (GDH), 3. Glutamine synthase fixes ammonia as glutamine. Hydrolytic release of the amide nitrogen of glutamine a ...
... 1. All the amino nitrogen from amino acids that undergo transamination can be concentrated in glutamate. 2. Release of nitrogen as ammonia is catalyzed by hepatic Lglutamate dehydrogenase (GDH), 3. Glutamine synthase fixes ammonia as glutamine. Hydrolytic release of the amide nitrogen of glutamine a ...
Enzymes - WordPress.com
... • Temperature coefficient or Q10 is a value for the reaction that shows how much the rate increases when you increase the temperature by 10oC • At temperatures before optimum if the Q10 is 2 then the rate doubles for 10oC increase • A value of 3 will triple the rate • Most enzymes are at 2 ...
... • Temperature coefficient or Q10 is a value for the reaction that shows how much the rate increases when you increase the temperature by 10oC • At temperatures before optimum if the Q10 is 2 then the rate doubles for 10oC increase • A value of 3 will triple the rate • Most enzymes are at 2 ...
Translation
... 1. Use Figure 22.3 to determine which template strand DNA sequence (written in the 5' → 3' direction) specifies the tripeptide with the sequence gly-ala-leu. A) GGGGCTCTC B) CTCTCGGGG C) CCCCGAGAG D) GAGAGCCCC 2. Which is not true about the genetic code? A) Some amino acids share the same codon. B) ...
... 1. Use Figure 22.3 to determine which template strand DNA sequence (written in the 5' → 3' direction) specifies the tripeptide with the sequence gly-ala-leu. A) GGGGCTCTC B) CTCTCGGGG C) CCCCGAGAG D) GAGAGCCCC 2. Which is not true about the genetic code? A) Some amino acids share the same codon. B) ...
FCH 532 Lecture 27
... glutamate gamma carboxyl group to an aldehyde (N-acetylglutamate-5-semialdehyde). Spontaneous cyclization is prevented by acetylation of amino group by Nacetylglutamate synthase. N-acetylglutamate-5-semialdehyde is converted to amine by transamination. Hydrolysis of protecting group yields ornithine ...
... glutamate gamma carboxyl group to an aldehyde (N-acetylglutamate-5-semialdehyde). Spontaneous cyclization is prevented by acetylation of amino group by Nacetylglutamate synthase. N-acetylglutamate-5-semialdehyde is converted to amine by transamination. Hydrolysis of protecting group yields ornithine ...
Physiological adaptations of Saccharomyces cerevisiae evolved for
... (v/v) being inhibitory for most cells including the native 1-butanol producers [14,16,17] while iso-butanol and 2butanol can be tolerated at higher levels [12,18]. One of the reasons for this is probably that 1-butanol is the most hydrophobic molecule with the strongest ability to permeate and/or in ...
... (v/v) being inhibitory for most cells including the native 1-butanol producers [14,16,17] while iso-butanol and 2butanol can be tolerated at higher levels [12,18]. One of the reasons for this is probably that 1-butanol is the most hydrophobic molecule with the strongest ability to permeate and/or in ...
Chicken eggshell matrix proteins related to anti
... negative bacteria, leading to death of the bacterium. This interaction could be promoted in OCX-36, since PROSITE analysis (http://www.ewpasy.org/tools/scanprosite) of its amino acid sequence identifies a PROKAR_LIPOPROTEIN motif. While the amino acid motif leading to S-acylation of eukaryotic prote ...
... negative bacteria, leading to death of the bacterium. This interaction could be promoted in OCX-36, since PROSITE analysis (http://www.ewpasy.org/tools/scanprosite) of its amino acid sequence identifies a PROKAR_LIPOPROTEIN motif. While the amino acid motif leading to S-acylation of eukaryotic prote ...
Integration of Mammalian Metabolism
... Maintainers: liver and adipose tissue Consumers: skeletal muscles, heart, brain ...
... Maintainers: liver and adipose tissue Consumers: skeletal muscles, heart, brain ...
3. The Molecules of Cells
... • Lactose intolerance illustrates the importance of biological molecules to the functioning of living cells and to human health • Molecular interactions, such as those between the gene for lactase production, the enzyme lactase, and the milk sugar lactose, drive all biological processes ...
... • Lactose intolerance illustrates the importance of biological molecules to the functioning of living cells and to human health • Molecular interactions, such as those between the gene for lactase production, the enzyme lactase, and the milk sugar lactose, drive all biological processes ...
Molecular and General Genetics
... Fig. 1. Comparison of the amino-terminal 75 amino acid residues of mature PhoE protein with the homologous region of OmpF protein according to Overbeeke et al. (]983). The complete proteins are 330 and 340 amino acids long, respectively. The amino acid sequence of PhoE protein is shown in the upper ...
... Fig. 1. Comparison of the amino-terminal 75 amino acid residues of mature PhoE protein with the homologous region of OmpF protein according to Overbeeke et al. (]983). The complete proteins are 330 and 340 amino acids long, respectively. The amino acid sequence of PhoE protein is shown in the upper ...
DNA Part II Lab
... o See attached supplementary files Give students a DNA sequence and ask them to determine the mRNA sequence, tRNA sequence, and amino acid sequence which would result from it. Have students alter their sequence just enough so that it results in a “mutation.” Lead a class discussion about genet ...
... o See attached supplementary files Give students a DNA sequence and ask them to determine the mRNA sequence, tRNA sequence, and amino acid sequence which would result from it. Have students alter their sequence just enough so that it results in a “mutation.” Lead a class discussion about genet ...
pptx
... Reaction 2: The carbons are transferred to lipoamide in a redox rxn (in E1’s active site) Hydroxyethyl-TPP·E1 ...
... Reaction 2: The carbons are transferred to lipoamide in a redox rxn (in E1’s active site) Hydroxyethyl-TPP·E1 ...
Question 2 (20 points)
... Compare ATP, NADH and NADPH with respect to their functions in metabolism. ...
... Compare ATP, NADH and NADPH with respect to their functions in metabolism. ...
P - GMC Surat
... Many proteins are initially made as initially made as large, precursor molecules that are not functionally active. Precursor proteins are cleaved in the endoplasmic reticulum or the Golgi apparatus, others are cleaved in developing secretory vesicles. Zymogens are inactive secreted enzymes (in ...
... Many proteins are initially made as initially made as large, precursor molecules that are not functionally active. Precursor proteins are cleaved in the endoplasmic reticulum or the Golgi apparatus, others are cleaved in developing secretory vesicles. Zymogens are inactive secreted enzymes (in ...
Haemoglobin.
... Hb has a strong tendency to combine with carbon monoxide forming a compound called carboxyhaemoglon. CO has almost 250 times stronger affinity for Hb than that of O2 for Hb. CO-Hb onces formed in the blood prevents formation of oxyhaemoglobin leading to hypoxia. Various pigments of bile, stool and u ...
... Hb has a strong tendency to combine with carbon monoxide forming a compound called carboxyhaemoglon. CO has almost 250 times stronger affinity for Hb than that of O2 for Hb. CO-Hb onces formed in the blood prevents formation of oxyhaemoglobin leading to hypoxia. Various pigments of bile, stool and u ...
Isolation, Properties and a Possible Function of a Water
... The rapeseed and radish proteins were postulated to function as an inhibitor of proteases since their amino acid sequences contain the signature motif of the Kiinitz family of protease inhibitors (Reviron et al. 1992, Lopez et al. 1994). This possibility could not be examined, however, because the p ...
... The rapeseed and radish proteins were postulated to function as an inhibitor of proteases since their amino acid sequences contain the signature motif of the Kiinitz family of protease inhibitors (Reviron et al. 1992, Lopez et al. 1994). This possibility could not be examined, however, because the p ...
A Conserved Family of Nuclear Proteins Containing
... Drosophila protein sequences deduced from the DNA sequences of serendipiry (Vincent et al., 1985) and of Kri@pel (Kr), a segmentation gene (Rosenberg et al., 1988). Aside from having in common the finger structure, these four and other finger proteins (see Berg, 1986) seem not to be directly related ...
... Drosophila protein sequences deduced from the DNA sequences of serendipiry (Vincent et al., 1985) and of Kri@pel (Kr), a segmentation gene (Rosenberg et al., 1988). Aside from having in common the finger structure, these four and other finger proteins (see Berg, 1986) seem not to be directly related ...
Unexpected Diversity of Signal Peptides in Prokaryotes
... ABSTRACT Signal peptides are a cornerstone mechanism for cellular protein localization, yet until now experimental determination of signal peptides has come from only a narrow taxonomic sampling. As a result, the dominant view is that Sec-cleaved signal peptides in prokaryotes are defined by a canon ...
... ABSTRACT Signal peptides are a cornerstone mechanism for cellular protein localization, yet until now experimental determination of signal peptides has come from only a narrow taxonomic sampling. As a result, the dominant view is that Sec-cleaved signal peptides in prokaryotes are defined by a canon ...
2-3
... such as olive oil, tend to be liquid at room temperature. Other cooking oils, such as corn oil, sesame oil, canola oil, and peanut oil, contain polyunsaturated lipids. ...
... such as olive oil, tend to be liquid at room temperature. Other cooking oils, such as corn oil, sesame oil, canola oil, and peanut oil, contain polyunsaturated lipids. ...
Proteolysis
Proteolysis is the breakdown of proteins into smaller polypeptides or amino acids. Uncatalysed, the hydrolysis of peptide bonds is extremely slow, taking hundreds of years. Proteolysis is typically catalysed by cellular enzymes called proteases, but may also occur by intra-molecular digestion. Low pH or high temperatures can also cause proteolysis non-enzymatically.Proteolysis in organisms serves many purposes; for example, digestive enzymes break down proteins in food to provide amino acids for the organism, while proteolytic processing of a polypeptide chain after its synthesis may be necessary for the production of an active protein. It is also important in the regulation of some physiological and cellular processes, as well as preventing the accumulation of unwanted or abnormal proteins in cells. Consequently, dis-regulation of proteolysis can cause diseases, and is used in some venoms to damage their prey.Proteolysis is important as an analytical tool for studying proteins in the laboratory, as well as industrially, for example in food processing and stain removal.