吴冬茵
... Remote homology detection: A central problem in computational biology, the classification of proteins into functional and structural classes given their amino acid sequences Discriminative method such as SVM is one of the most effective methods Explicit feature are usually large and noise data ...
... Remote homology detection: A central problem in computational biology, the classification of proteins into functional and structural classes given their amino acid sequences Discriminative method such as SVM is one of the most effective methods Explicit feature are usually large and noise data ...
... Ala Ile Asn (pH between 0 and 10), or polar. Some amino acids belong to Trp His Phe Gln more than one set, e.g. all charged residues are also polar. Thr a) Explain, with reference to its sidechain atoms, where you Ser would place Tyrosine (Tyr) on this chart (2 pts). Polar b) One amino acid is very ...
Protein Foods
... What is the difference? o Complete: Animal sources of protein. These proteins contain all of the protein building blocks called amino acids that your body needs to grow and maintain tissue. o Incomplete: Plant sources of protein. These proteins are missing one or more amino acids; not all of the bui ...
... What is the difference? o Complete: Animal sources of protein. These proteins contain all of the protein building blocks called amino acids that your body needs to grow and maintain tissue. o Incomplete: Plant sources of protein. These proteins are missing one or more amino acids; not all of the bui ...
Molecular Components of the Signal Sequence that Function in the
... One of the characteristic phenotypes exhibited by strains carrying the 42-1 fusion is related to the inability of the cell to export the LamB-LacZ hybrid protein efficiently. When cells containing this gene fusion are grown in the presence of maltose to induce high-level synthesis of the hybrid prot ...
... One of the characteristic phenotypes exhibited by strains carrying the 42-1 fusion is related to the inability of the cell to export the LamB-LacZ hybrid protein efficiently. When cells containing this gene fusion are grown in the presence of maltose to induce high-level synthesis of the hybrid prot ...
HOW TO USE BAD: A BRIEF PRESENTATION
... fibronectin 3.1%; ribonuclease 3%; cholesterol esterase 2.6%; alpha-chymotrypsin 2.2%; insulin 2.1%; beta- casein 2.1%; cutinase 1.7 %; human growth hormone 0.8%; immunoglobulin M 0.8%; alpha-2-macroglobulin 0.8%; alpha-s1-casein 0.8%; betalactoglobulin 0.8%; protein C3 0.5%; alpha-1-acid glycoprote ...
... fibronectin 3.1%; ribonuclease 3%; cholesterol esterase 2.6%; alpha-chymotrypsin 2.2%; insulin 2.1%; beta- casein 2.1%; cutinase 1.7 %; human growth hormone 0.8%; immunoglobulin M 0.8%; alpha-2-macroglobulin 0.8%; alpha-s1-casein 0.8%; betalactoglobulin 0.8%; protein C3 0.5%; alpha-1-acid glycoprote ...
tethering redox proteins to the outer membrane in Neisseria and
... from the surface of the inner leaflet of the outer membrane. Hong et al. [18] showed that the Laz protein or a GST (glutathione transferase) fused to the signal peptide plus Nterminal LCR of Laz enables the globular azurin–GST to be surface-exposed in Neisseria (or indeed on expression in E. coli). ...
... from the surface of the inner leaflet of the outer membrane. Hong et al. [18] showed that the Laz protein or a GST (glutathione transferase) fused to the signal peptide plus Nterminal LCR of Laz enables the globular azurin–GST to be surface-exposed in Neisseria (or indeed on expression in E. coli). ...
Phenylalanineaminopeptidase of L. pneumophila
... is characteristic of bacterial thiol-dependent serine proteinases (Stepanov et al., 1981). Only 20% of the total activity against azocasein was inhibited by di-isopropyl fluorophosphate, whereas 80% of the activity was inhibited by Na2EDTA, suggesting that L. pneumophila produces metalloproteinase(s ...
... is characteristic of bacterial thiol-dependent serine proteinases (Stepanov et al., 1981). Only 20% of the total activity against azocasein was inhibited by di-isopropyl fluorophosphate, whereas 80% of the activity was inhibited by Na2EDTA, suggesting that L. pneumophila produces metalloproteinase(s ...
medical management: portosystemic vascular anomalies (psva)
... systemic oxidative deamination of amino acids derived from the gut. Enteric ammonia also derives from bacterial hydrolysis of urea (ureases). Constipation as well as blood within the digestive tract each increase enteric ammonia and amino acid production and substantially contribute to HE (Table 2). ...
... systemic oxidative deamination of amino acids derived from the gut. Enteric ammonia also derives from bacterial hydrolysis of urea (ureases). Constipation as well as blood within the digestive tract each increase enteric ammonia and amino acid production and substantially contribute to HE (Table 2). ...
Document
... • Over long periods of time a sequence will acquire random mutations. – These mutations may result in a new amino acid at a given position, the deletion of an amino acid, or the introduction of a new one. – Over VERY long periods of time two sequences may diverge so much that their relationship can ...
... • Over long periods of time a sequence will acquire random mutations. – These mutations may result in a new amino acid at a given position, the deletion of an amino acid, or the introduction of a new one. – Over VERY long periods of time two sequences may diverge so much that their relationship can ...
Polycystin-2 takes different routes to the somatic and ciliary plasma
... polycystin-2, pulse-chase experiments were performed in HeLa cells (another nonciliated cell line) and in LLC-PK1 cells. By radioactively labeling the cells for a short period of 5 min we were able to follow the fate of newly synthesized polycystin-2 for up to 24 h. Except for the appearance of a sl ...
... polycystin-2, pulse-chase experiments were performed in HeLa cells (another nonciliated cell line) and in LLC-PK1 cells. By radioactively labeling the cells for a short period of 5 min we were able to follow the fate of newly synthesized polycystin-2 for up to 24 h. Except for the appearance of a sl ...
Lipid Biosynthesis
... B) Rearrangement. C) Reduction. D) Dehydration. 3. Which of the following is the regulated step of fatty acid synthesis in eukaryotes? A) Carboxylation of acetyl CoA. B) Transportation of mitochondrial acetyl CoA into the cytosol. C) Assembly of the fatty acid chain. D) All of the above. ...
... B) Rearrangement. C) Reduction. D) Dehydration. 3. Which of the following is the regulated step of fatty acid synthesis in eukaryotes? A) Carboxylation of acetyl CoA. B) Transportation of mitochondrial acetyl CoA into the cytosol. C) Assembly of the fatty acid chain. D) All of the above. ...
Mutations in the VPS45 gene, a SEC1 homologue, result in vacuolar
... Recombinant DNA manipulations employed in the construction of plasmids were performed as described previously (Maniatis et al., 1989), with the exception of DNA fragment isolations carried out by the glass bead method of Vogelstein and Gillespie (1979). The CENbased plasmid pVPS45-10 was generated b ...
... Recombinant DNA manipulations employed in the construction of plasmids were performed as described previously (Maniatis et al., 1989), with the exception of DNA fragment isolations carried out by the glass bead method of Vogelstein and Gillespie (1979). The CENbased plasmid pVPS45-10 was generated b ...
Biochemistry I, Spring Term 2005 - Second Exam:
... b) Drugs that are good inhibitors cannot by synthesized. c) The drugs are rapidly degraded. d) Virus particles with altered (mutant) proteases arise. 8. During any purification scheme, you would expect a) the number of different proteins in the sample to decrease. b) that the specific activity incre ...
... b) Drugs that are good inhibitors cannot by synthesized. c) The drugs are rapidly degraded. d) Virus particles with altered (mutant) proteases arise. 8. During any purification scheme, you would expect a) the number of different proteins in the sample to decrease. b) that the specific activity incre ...
Elucidating the complete reaction cycle for membrane
... This potential can be used to drive other cellular reactions such as ATP synthesis and the primary active transport of solutes. MPPases are found in bacteria, archaea, protozoans and plants, but not in mammals. Crucially, MPPases are found in numerous bacterial and protozoan parasites such as Plasmo ...
... This potential can be used to drive other cellular reactions such as ATP synthesis and the primary active transport of solutes. MPPases are found in bacteria, archaea, protozoans and plants, but not in mammals. Crucially, MPPases are found in numerous bacterial and protozoan parasites such as Plasmo ...
WrkSht4-AAroles-Mutations
... C-terminus of a helix and also H-bonds to one of the free backbone CO groups. Zoom out to see that helix; its Ncap is residue ____________ ____________ (for a double blank, please give the residue name and number). [Remember that the cap residue C is the dividing point between peptides clearly in th ...
... C-terminus of a helix and also H-bonds to one of the free backbone CO groups. Zoom out to see that helix; its Ncap is residue ____________ ____________ (for a double blank, please give the residue name and number). [Remember that the cap residue C is the dividing point between peptides clearly in th ...
Chapter 10 - Protein Synthesis: Transcription and Translation
... • RNA polymerase• adds RNA nucleotides complimentary to the DNA template strand • Transcription factors• proteins that recognize specific sequences in DNA when making mRNA and help RNA polymerase bind • ATPase• converts ATP to ADP and releases energy to do work ( used to bond tRNA to mRNA and GTPase ...
... • RNA polymerase• adds RNA nucleotides complimentary to the DNA template strand • Transcription factors• proteins that recognize specific sequences in DNA when making mRNA and help RNA polymerase bind • ATPase• converts ATP to ADP and releases energy to do work ( used to bond tRNA to mRNA and GTPase ...
Chapter 26:Biomolecules: Amino Acids, Peptides, and Proteins
... congregate on the hydrocarbon-like interior of a protein molecule Also important for stabilizing a protein's tertiary structure are the formation of disulfide bridges between cysteine residues, the formation of hydrogen bonds between nearby amino acid residues, and the development of ionic attract ...
... congregate on the hydrocarbon-like interior of a protein molecule Also important for stabilizing a protein's tertiary structure are the formation of disulfide bridges between cysteine residues, the formation of hydrogen bonds between nearby amino acid residues, and the development of ionic attract ...
Protein damage on Cu2+
... MCO reactions during IMAC: Presence of hydroxyl radicals – An MCO reaction which includes the Fenton Reaction gives rise to oxidizing species such as OH radical in the reaction between the metal and the peroxide. – The presence of OH radicals indicates that a Fenton reaction (or some other radical g ...
... MCO reactions during IMAC: Presence of hydroxyl radicals – An MCO reaction which includes the Fenton Reaction gives rise to oxidizing species such as OH radical in the reaction between the metal and the peroxide. – The presence of OH radicals indicates that a Fenton reaction (or some other radical g ...
Nutritional Biochemistry
... Some fatty acids are linoleic, linolenic, and stearic. An acid group, also called carboxylic group has a formula of COOH. The part of the molecule with an acid group is water soluble. A methyl group has a formula of CH3 and that portion is lipid soluble. A hydrocarbon is a combination of methyl grou ...
... Some fatty acids are linoleic, linolenic, and stearic. An acid group, also called carboxylic group has a formula of COOH. The part of the molecule with an acid group is water soluble. A methyl group has a formula of CH3 and that portion is lipid soluble. A hydrocarbon is a combination of methyl grou ...
An NIH funded center led by deCODE biostructures and
... fellows, scientific and other technicians, doctoral degree graduate students, and administrative and technical support personnel in 14 buildings overlooking the Pacific Ocean in La Jolla, a part of the City of San Diego. Scripps Florida will be a 350,000 square-foot, state-of-the-art biomedical rese ...
... fellows, scientific and other technicians, doctoral degree graduate students, and administrative and technical support personnel in 14 buildings overlooking the Pacific Ocean in La Jolla, a part of the City of San Diego. Scripps Florida will be a 350,000 square-foot, state-of-the-art biomedical rese ...
PPT - Surekha Bhargava
... Features & Benefits Proteins are vital for the sustenance of life. they are one of the most important nutrients required by the body. they form the important component of muscle and other tissues and vital body fluids like blood. ...
... Features & Benefits Proteins are vital for the sustenance of life. they are one of the most important nutrients required by the body. they form the important component of muscle and other tissues and vital body fluids like blood. ...
Proteolysis
Proteolysis is the breakdown of proteins into smaller polypeptides or amino acids. Uncatalysed, the hydrolysis of peptide bonds is extremely slow, taking hundreds of years. Proteolysis is typically catalysed by cellular enzymes called proteases, but may also occur by intra-molecular digestion. Low pH or high temperatures can also cause proteolysis non-enzymatically.Proteolysis in organisms serves many purposes; for example, digestive enzymes break down proteins in food to provide amino acids for the organism, while proteolytic processing of a polypeptide chain after its synthesis may be necessary for the production of an active protein. It is also important in the regulation of some physiological and cellular processes, as well as preventing the accumulation of unwanted or abnormal proteins in cells. Consequently, dis-regulation of proteolysis can cause diseases, and is used in some venoms to damage their prey.Proteolysis is important as an analytical tool for studying proteins in the laboratory, as well as industrially, for example in food processing and stain removal.