Rapid increase of cytosolic content of acetyl-CoA
... Mammalian acetyl-CoA carboxylase (ACC) is present in two isoforms, and , both of which catalyze formation of malonyl-CoA by fixing CO2 into acetylCoA. ACC- is highly expressed in lipogenic tissues whereas ACC- is a predominant form in heart and skeletal muscle tissues. Even though the tissuespecific ...
... Mammalian acetyl-CoA carboxylase (ACC) is present in two isoforms, and , both of which catalyze formation of malonyl-CoA by fixing CO2 into acetylCoA. ACC- is highly expressed in lipogenic tissues whereas ACC- is a predominant form in heart and skeletal muscle tissues. Even though the tissuespecific ...
Brooker Chapter 14
... Negative control refers to transcriptional regulation by repressor proteins Positive control to regulation by activator proteins Copyright ©The McGraw-Hill Companies, Inc. Permission required for reproduction or display ...
... Negative control refers to transcriptional regulation by repressor proteins Positive control to regulation by activator proteins Copyright ©The McGraw-Hill Companies, Inc. Permission required for reproduction or display ...
... Figure 3. Concentration-dependent increases in leucine transport by mouse and bovine mammary epithelial cells. HC11 cells (mouse mammary epithelial cells) and Mac-T cells (bovine mammary epithelial cells) were obtained from American Type Culture Collection (Manassa, VA). Approximately 1×106 viable c ...
Pepsin A (2F5): sc-101405 - Santa Cruz Biotechnology
... Pepsin is one of the main proteolytic enzymes secreted by the gastric mucosa. Pepsin consists of a single polypeptide chain and arises from its precursor, pepsinogen, by removal of a 41 amino acid segment from the N-terminus. Pepsinogen is synthesized in the stomach lining, and hydrochloric acid, al ...
... Pepsin is one of the main proteolytic enzymes secreted by the gastric mucosa. Pepsin consists of a single polypeptide chain and arises from its precursor, pepsinogen, by removal of a 41 amino acid segment from the N-terminus. Pepsinogen is synthesized in the stomach lining, and hydrochloric acid, al ...
INTRODUCTION
... ribosomes. These must be targeted to the organelle and then taken up and sorted to the correct compartment. Proteins destined for mitochondria are made as precursors that have both a targeting signal to direct them to receptors on the mitochondrial surface and intramitochondrial sorting signals. At ...
... ribosomes. These must be targeted to the organelle and then taken up and sorted to the correct compartment. Proteins destined for mitochondria are made as precursors that have both a targeting signal to direct them to receptors on the mitochondrial surface and intramitochondrial sorting signals. At ...
Document
... What interferes with nitrogen fixation? Oxygen is the major factor. Nitrogen fixation can take place only in the total absence of O2 How does a plant overcome oxygen interference? By synthesizing a heme protein, leghemoglobin, which like other hemoglobins, has a high affinity for binding oxygen….wh ...
... What interferes with nitrogen fixation? Oxygen is the major factor. Nitrogen fixation can take place only in the total absence of O2 How does a plant overcome oxygen interference? By synthesizing a heme protein, leghemoglobin, which like other hemoglobins, has a high affinity for binding oxygen….wh ...
LECT 29 NitrogFix
... What interferes with nitrogen fixation? Oxygen is the major factor. Nitrogen fixation can take place only in the total absence of O2 How does a plant overcome oxygen interference? By synthesizing a heme protein, leghemoglobin, which like other hemoglobins, has a high affinity for binding oxygen….wh ...
... What interferes with nitrogen fixation? Oxygen is the major factor. Nitrogen fixation can take place only in the total absence of O2 How does a plant overcome oxygen interference? By synthesizing a heme protein, leghemoglobin, which like other hemoglobins, has a high affinity for binding oxygen….wh ...
Biochemistry - Grade12BiologyCALC
... protein carriers involves the binding of specific • Both move polar molecules to protein carriers that are highly molecules down their specific (like enzymes are with their concentration gradient substrates) • The specific nature of facilitated diffusion is from [high] to [low] way for the cell to t ...
... protein carriers involves the binding of specific • Both move polar molecules to protein carriers that are highly molecules down their specific (like enzymes are with their concentration gradient substrates) • The specific nature of facilitated diffusion is from [high] to [low] way for the cell to t ...
Biochemical fossils of the ancient transition from geoenergetics to
... acetogen and methanogen structures are most closely related. This was done by submitting the coordinates of the PDB entries corresponding to reactions 1–13 in Fig. 4 to a structural similarity search at the DALI server to find its structural homologues. This revealed whether the acetogen and methanog ...
... acetogen and methanogen structures are most closely related. This was done by submitting the coordinates of the PDB entries corresponding to reactions 1–13 in Fig. 4 to a structural similarity search at the DALI server to find its structural homologues. This revealed whether the acetogen and methanog ...
Module Number- 3181
... Written evidence of the ability to give the required representations of amino acids and proteins and to describe specified properties, role of proteins and the role of enzyme given with performance Criteria (a) to (d) respectively. Performance evidence of the ability to analyse amino acids which wil ...
... Written evidence of the ability to give the required representations of amino acids and proteins and to describe specified properties, role of proteins and the role of enzyme given with performance Criteria (a) to (d) respectively. Performance evidence of the ability to analyse amino acids which wil ...
The common northern periwinkle, Littorina littorea
... Under aerobic conditions, organisms can make use of lipid, carbohydrate or amino acid fuels for respiration with considerable variation between species and between organs in the relative importance of different fuel types. Under anoxic conditions, however, carbohydrates become the primary substrate ...
... Under aerobic conditions, organisms can make use of lipid, carbohydrate or amino acid fuels for respiration with considerable variation between species and between organs in the relative importance of different fuel types. Under anoxic conditions, however, carbohydrates become the primary substrate ...
PDF
... amplified DNA was inserted into the rhamnose promoter vector pRham containing an N terminal histidine tag and transformed into 10G competent E. coli cells (Lucigen Corp.). Recombinant Dtur DNAP production was induced by rhamnose and the enzyme was purified using standard methods for His-tagged prote ...
... amplified DNA was inserted into the rhamnose promoter vector pRham containing an N terminal histidine tag and transformed into 10G competent E. coli cells (Lucigen Corp.). Recombinant Dtur DNAP production was induced by rhamnose and the enzyme was purified using standard methods for His-tagged prote ...
Predicting drug-target interaction in cancers using homology
... response to drugs. Our aim was towards the understanding of the possible role of structural variations in breast cancer drug metabolising gene such as MTHFR that closely interact with the neoplastic drugs viz. Cyclophosphamide, 5-Fluorouracil, Methotrexate and others. We investigated the polymorphis ...
... response to drugs. Our aim was towards the understanding of the possible role of structural variations in breast cancer drug metabolising gene such as MTHFR that closely interact with the neoplastic drugs viz. Cyclophosphamide, 5-Fluorouracil, Methotrexate and others. We investigated the polymorphis ...
Expression profiles of Ciona tailbud embryos - Development
... multiple tissues. The study also highlighted characteristic gene expression profiles dependent on the tissues. In addition, several genes showed intriguing expression patterns that have not been reported previously; for example, four genes were expressed specifically in the nerve cord cells and one ...
... multiple tissues. The study also highlighted characteristic gene expression profiles dependent on the tissues. In addition, several genes showed intriguing expression patterns that have not been reported previously; for example, four genes were expressed specifically in the nerve cord cells and one ...
Practical part
... are reversible and irreversible (denaturation) methods for the precipitation of proteins. The reversible precipitation occurs with high concentration of neutral salts (salting out), alcohol, and acetone (the last two conducted at below zero temperatures). The mechanism of precipitation consists in r ...
... are reversible and irreversible (denaturation) methods for the precipitation of proteins. The reversible precipitation occurs with high concentration of neutral salts (salting out), alcohol, and acetone (the last two conducted at below zero temperatures). The mechanism of precipitation consists in r ...
Role of Liver In Triglyceride Homeostasis
... • Enhances transcription of genes required for FA synthesis • Responsive genes include: ...
... • Enhances transcription of genes required for FA synthesis • Responsive genes include: ...
Protein phosphorylation in chloroplasts – a survey of
... fragmentation at slightly elevated collision energies in a procedure that is referred to as multistage activation (MSA). This eradicates the neutral loss peak and generates higher intensity product ions (b- or y-ions, see Fig. 1 for an explanation) for spectra interpretation and phosphorylation site ...
... fragmentation at slightly elevated collision energies in a procedure that is referred to as multistage activation (MSA). This eradicates the neutral loss peak and generates higher intensity product ions (b- or y-ions, see Fig. 1 for an explanation) for spectra interpretation and phosphorylation site ...
Does Sequence Dictate Structure Which Dictates Function?
... throughout (Figure 1A). Homology is all about functional relatedness; it is all or nothing, but not at all equivalent to either sequence similarity or even structural similarity. In fact, homology is the single most important requirement for the success of comparative sequence analysis, the most pow ...
... throughout (Figure 1A). Homology is all about functional relatedness; it is all or nothing, but not at all equivalent to either sequence similarity or even structural similarity. In fact, homology is the single most important requirement for the success of comparative sequence analysis, the most pow ...
Visualizza/apri
... were separated and then quantified in relative terms. In particular, the activity of μ-calpain and caspase-3 was assessed by the quantification of alpha II spectrin cleavage products of 145 kDa (SBDP145) and of 120 kDa (SBDP120) respectively. Moreover the activity of caspase-3 was assessed also by t ...
... were separated and then quantified in relative terms. In particular, the activity of μ-calpain and caspase-3 was assessed by the quantification of alpha II spectrin cleavage products of 145 kDa (SBDP145) and of 120 kDa (SBDP120) respectively. Moreover the activity of caspase-3 was assessed also by t ...
Redox regulation of cysteine
... There are 2 main forms of SOD in mammalian cells, MnSOD (SOD2; localized to mitochondria) and CuZnSOD, with 2 types of CuZnSOD that are found either intracellularly (SOD1) or extracellularly (SOD3). These enzymes are responsible for the conversion of superoxide to H2O2, although this occurs slowly a ...
... There are 2 main forms of SOD in mammalian cells, MnSOD (SOD2; localized to mitochondria) and CuZnSOD, with 2 types of CuZnSOD that are found either intracellularly (SOD1) or extracellularly (SOD3). These enzymes are responsible for the conversion of superoxide to H2O2, although this occurs slowly a ...
ETC_2012 Quiz
... Presenting complaint: However, she started complaining of a burning sensation in her throat and mouth which was followed by nausea and vomiting, diaphoresis, agitation, and dyspnea. On Examination: The nurse noticed an almond-like smell in her breath. Lab investigations: An arterial blood gas reveal ...
... Presenting complaint: However, she started complaining of a burning sensation in her throat and mouth which was followed by nausea and vomiting, diaphoresis, agitation, and dyspnea. On Examination: The nurse noticed an almond-like smell in her breath. Lab investigations: An arterial blood gas reveal ...
Site-selective incorporation and ligation of
... the N-terminal aspartate residue (generating a ketone) under mild conditions ( pH 6.5 PB, 18 hours, 37 °C). The N-terminal ketone generated from this biomimetic transformation can then be site-selectively modified further. PLP-mediated transamination was successfully demonstrated on a number of prot ...
... the N-terminal aspartate residue (generating a ketone) under mild conditions ( pH 6.5 PB, 18 hours, 37 °C). The N-terminal ketone generated from this biomimetic transformation can then be site-selectively modified further. PLP-mediated transamination was successfully demonstrated on a number of prot ...
A study of archaeal enzymes involved in polar lipid
... enzymes have not been fully examined. Through database searching, we detected many archaeal hypothetical proteins that show sequence similarity to members of the CDP alcohol phosphatidyltransferase family, such as phosphatidylserine synthase (PSS), phosphatidylglycerol synthase (PGS) and phosphatidy ...
... enzymes have not been fully examined. Through database searching, we detected many archaeal hypothetical proteins that show sequence similarity to members of the CDP alcohol phosphatidyltransferase family, such as phosphatidylserine synthase (PSS), phosphatidylglycerol synthase (PGS) and phosphatidy ...
Proteolysis
Proteolysis is the breakdown of proteins into smaller polypeptides or amino acids. Uncatalysed, the hydrolysis of peptide bonds is extremely slow, taking hundreds of years. Proteolysis is typically catalysed by cellular enzymes called proteases, but may also occur by intra-molecular digestion. Low pH or high temperatures can also cause proteolysis non-enzymatically.Proteolysis in organisms serves many purposes; for example, digestive enzymes break down proteins in food to provide amino acids for the organism, while proteolytic processing of a polypeptide chain after its synthesis may be necessary for the production of an active protein. It is also important in the regulation of some physiological and cellular processes, as well as preventing the accumulation of unwanted or abnormal proteins in cells. Consequently, dis-regulation of proteolysis can cause diseases, and is used in some venoms to damage their prey.Proteolysis is important as an analytical tool for studying proteins in the laboratory, as well as industrially, for example in food processing and stain removal.