2–3 Carbon Compounds
... The portion of each amino acid that is different is a side chain called an R-group. ...
... The portion of each amino acid that is different is a side chain called an R-group. ...
Exercises and Solutions
... • Then go back to the top of the flatfile and click Include FRENDA results (AMENDA + additional results, but less precise; more...) • Again open the menue organism-related information on the navigation bar and look at the section „organism“. What is different now? • The optimal temperature for RUBIS ...
... • Then go back to the top of the flatfile and click Include FRENDA results (AMENDA + additional results, but less precise; more...) • Again open the menue organism-related information on the navigation bar and look at the section „organism“. What is different now? • The optimal temperature for RUBIS ...
Analyzing Effects of Naturally Occurring Missense Mutations
... and Aggregation. Protein folding is the process of converting the linear unfolded polypeptide into the native 3D structure driven by the gradient of potential energy [80, 81]. The importance of kinetics of protein folding is manifested by the fact that protein miss-folding is involved in many diseas ...
... and Aggregation. Protein folding is the process of converting the linear unfolded polypeptide into the native 3D structure driven by the gradient of potential energy [80, 81]. The importance of kinetics of protein folding is manifested by the fact that protein miss-folding is involved in many diseas ...
Microsecond Rotational Dynamics of Spin-Labeled Ca
... acid and 4 mM cold inorganic phosphate. The solution was filtered through a 0.45-pm Millipore filter, and the filter was washed with ice-cold perchloric acid and phosphate. The filter was then counted in a scintillation counter. A background blank (prepared in the same way as the assay tube except i ...
... acid and 4 mM cold inorganic phosphate. The solution was filtered through a 0.45-pm Millipore filter, and the filter was washed with ice-cold perchloric acid and phosphate. The filter was then counted in a scintillation counter. A background blank (prepared in the same way as the assay tube except i ...
Enzymes are macromolecules that help accelerate (catalyze
... to that of the substrate only after the substrate is bound. This process of dynamic recognition is called induced fit. Enzymes are highly specific and may require cofactors for catalysis. A cofactor is a nonprotein chemical compound bound to a protein; there are 2 types of cofactors: Metals and orga ...
... to that of the substrate only after the substrate is bound. This process of dynamic recognition is called induced fit. Enzymes are highly specific and may require cofactors for catalysis. A cofactor is a nonprotein chemical compound bound to a protein; there are 2 types of cofactors: Metals and orga ...
Sequence Specific Modeling of E. coli Cell-Free Protein
... organisms, including industrially important prokaryotes such as E. coli (19) and B. subtilis (20), are now available (21). Stoichiometric reconstructions have been expanded to include the integration of metabolism with detailed descriptions of gene expression (ME-Model) (17, 22) and protein structur ...
... organisms, including industrially important prokaryotes such as E. coli (19) and B. subtilis (20), are now available (21). Stoichiometric reconstructions have been expanded to include the integration of metabolism with detailed descriptions of gene expression (ME-Model) (17, 22) and protein structur ...
Quantitative parameters for amino acid–base
... factor zif268, and vice versa (2,3,11–13). zif268, which belongs to the Cys2His2 family of zinc finger proteins, provides a convenient system to study the specificity of recognition between an amino acid and a DNA base. This is due to the simplicity of its structure and mode of interaction with DNA. ...
... factor zif268, and vice versa (2,3,11–13). zif268, which belongs to the Cys2His2 family of zinc finger proteins, provides a convenient system to study the specificity of recognition between an amino acid and a DNA base. This is due to the simplicity of its structure and mode of interaction with DNA. ...
Aromatic amino acid catabolism by lactococci
... development, the pathways present in cheese microflora are poorly understood. To determine the pathways of aromatic amino acid catabolism in lactococci and effects of Cheddar cheese ripening conditions on catabolic enzymes and products, eight starter lactococcal strains were screened. Cell-free extr ...
... development, the pathways present in cheese microflora are poorly understood. To determine the pathways of aromatic amino acid catabolism in lactococci and effects of Cheddar cheese ripening conditions on catabolic enzymes and products, eight starter lactococcal strains were screened. Cell-free extr ...
Protein enzyme
... No reaction takes place on the inhibitor Inhibition depends on the strength of inhibitor binding and inhibitor concentration Substrate is blocked from the active site Increasing substrate concentration reverses inhibition Inhibitor likely to be similar in structure to the substrate ...
... No reaction takes place on the inhibitor Inhibition depends on the strength of inhibitor binding and inhibitor concentration Substrate is blocked from the active site Increasing substrate concentration reverses inhibition Inhibitor likely to be similar in structure to the substrate ...
(De)regulation of key enzyme steps in the shikimate pathway and
... CM protein, thus forming a heteromeric two-enzyme complex. The two enzyme activities can be separated by Q-Sepharose anion-exchange chromatography, yielding a dimeric CM protein with a fivefold reduced activity that is no longer feedback-inhibited by Phe and Tyr, and a 160 kDa DAHP synthase that is ...
... CM protein, thus forming a heteromeric two-enzyme complex. The two enzyme activities can be separated by Q-Sepharose anion-exchange chromatography, yielding a dimeric CM protein with a fivefold reduced activity that is no longer feedback-inhibited by Phe and Tyr, and a 160 kDa DAHP synthase that is ...
Full Paper - Biotechniques.org
... this paper shows high homology to previously reported sequences of the PEX5 gene. Although the 1.2kb sequence reported does not represent the entire PEX5 gene, it provides evidence that such a homologue does exist in rats, and that the techniques reported herein are sufficient to isolate and identif ...
... this paper shows high homology to previously reported sequences of the PEX5 gene. Although the 1.2kb sequence reported does not represent the entire PEX5 gene, it provides evidence that such a homologue does exist in rats, and that the techniques reported herein are sufficient to isolate and identif ...
Lecture 10 Thurs 4-27-06
... 1. Adhesion to vascular endothelium is a key factor in pathogenicity and is dependent on the Plasmodium protein PfEMP1 and endothelial receptors including CD36. 2. Evidence that binding of IRBCs to CD36 on endothelial cells activates a signaling pathway important for cytoadherence (From Yipp, B. et ...
... 1. Adhesion to vascular endothelium is a key factor in pathogenicity and is dependent on the Plasmodium protein PfEMP1 and endothelial receptors including CD36. 2. Evidence that binding of IRBCs to CD36 on endothelial cells activates a signaling pathway important for cytoadherence (From Yipp, B. et ...
Basic mechanisms of normal and abnormal
... (i.e., salivary glands, liver, and pancreas) that empty their contents into the canal. In a general sense, the GI tract adds water, ions, and enzymes to a meal to convert it into an aqueous solution of molecules that can be transported within the body. Importantly, most of the added substances are a ...
... (i.e., salivary glands, liver, and pancreas) that empty their contents into the canal. In a general sense, the GI tract adds water, ions, and enzymes to a meal to convert it into an aqueous solution of molecules that can be transported within the body. Importantly, most of the added substances are a ...
TAR-RNA binding by HIV-1 Tat protein is
... (a). Shift intensity is plotted as a function of concentration of RNA competitor as indicated. ...
... (a). Shift intensity is plotted as a function of concentration of RNA competitor as indicated. ...
Topology Prediction of Membrane Proteins
... lipids, which separates them from the outside world. The membrane is a physical barrier that protects the cell from foreign molecules at the same time as it prevents leakage of internal components and substances. However, a cell must be able to communicate with its surroundings, exchange molecules a ...
... lipids, which separates them from the outside world. The membrane is a physical barrier that protects the cell from foreign molecules at the same time as it prevents leakage of internal components and substances. However, a cell must be able to communicate with its surroundings, exchange molecules a ...
chapt 8
... By increasing or decreasing the rate of transcription of the gene by enhancer and silencer regions on the DNA ...
... By increasing or decreasing the rate of transcription of the gene by enhancer and silencer regions on the DNA ...
Phosphate Groups Modifying Myelin Basic Proteins Are
... The time course of the appearance and decay of the radioactive label on basic proteins in isolated myelin was followed for 1 too. Incorporation was maximal by 1 h, followed by a decay phase with a half-life of approximately 2 wk. However, radioactivity in the acid-soluble precursor pool (which alway ...
... The time course of the appearance and decay of the radioactive label on basic proteins in isolated myelin was followed for 1 too. Incorporation was maximal by 1 h, followed by a decay phase with a half-life of approximately 2 wk. However, radioactivity in the acid-soluble precursor pool (which alway ...
Abstract-- Lactic acid bacteria are characterized
... presence of Met-Pro or Leu-Pro as source of methionine or leucine respectively, the final cell concentration was 20% lower as regards to basal medium (Fig. 2A). The growth rates (Fig. 2B) were identical to that obtained in basal medium, except when Gly was added as part of the dipeptide Gly-Gly. In ...
... presence of Met-Pro or Leu-Pro as source of methionine or leucine respectively, the final cell concentration was 20% lower as regards to basal medium (Fig. 2A). The growth rates (Fig. 2B) were identical to that obtained in basal medium, except when Gly was added as part of the dipeptide Gly-Gly. In ...
Proteolysis
Proteolysis is the breakdown of proteins into smaller polypeptides or amino acids. Uncatalysed, the hydrolysis of peptide bonds is extremely slow, taking hundreds of years. Proteolysis is typically catalysed by cellular enzymes called proteases, but may also occur by intra-molecular digestion. Low pH or high temperatures can also cause proteolysis non-enzymatically.Proteolysis in organisms serves many purposes; for example, digestive enzymes break down proteins in food to provide amino acids for the organism, while proteolytic processing of a polypeptide chain after its synthesis may be necessary for the production of an active protein. It is also important in the regulation of some physiological and cellular processes, as well as preventing the accumulation of unwanted or abnormal proteins in cells. Consequently, dis-regulation of proteolysis can cause diseases, and is used in some venoms to damage their prey.Proteolysis is important as an analytical tool for studying proteins in the laboratory, as well as industrially, for example in food processing and stain removal.