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Chemistry of Cells
Chemistry of Cells

... Proteins are instrumental in about everything that an organism does. ...
Scientists clarify structural basis for biosynthesis of mysterious 21st
Scientists clarify structural basis for biosynthesis of mysterious 21st

... converted into proteins in cells, is its universality: nucleotide triplets (“codons”) encode a set of twenty amino acids that form the building blocks for all living organisms. Selenocysteine, the “21st amino acid” whose antioxidant properties help prevent cellular damage, is a rare exception to thi ...
Recombinant Ebola virus VP40 matrix protein
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... Storage: -80°C. It is recommended to dispense single-use aliquots and store aliquots at -80°C to avoid multiple freeze/thaw cycles. Size: 100 µg of protein is supplied in HEPES buffer pH 7.5 containing sodium chloride, 5% glycerol and 0.1% Triton-X, at a concentration of 1.602 mg/mL. The theoretical ...
Finch Soft Food
Finch Soft Food

... grasses and plants provide a rich diversity of nutrients compared to the cereal grains that are usually fed in captivity. Many seeds found in commercial finch mixes contain low quality protein and are deficient in essential amino acids, particularly lysine and methionine. In the wild, finches often ...
DNA and RNA
DNA and RNA

... • Protein is released from ribosome when “stop codon” is reached ...
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... domain (amino acids 1-130 (32-99 according to SwissProt) which mediates homo-dimerization and proteinprotein interactions with other corepressors (including HDAC1 and NCOR2/SMRT ) to constitute a large repressing complex, another transcription repression domain (191-386), PEST sequences (300-417) wi ...
Chapter 2 Review Sheet Name:_______________________
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... Nucleic Acid - “hard drive”of the cell; - holds the directions to make proteins - MASTER copy of all ...
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... 1005 amino acids, 125 kDa; membrane restricted; 448 N-term amino acid from MSN, containing the band 4.1 like domain and most of the alpha helix domain, fused to the 557 (instead of the usual 562) C-term amino acids from ALK (i.e. the cytoplasmic portion of ALK with the tyrosine kinase domain). Oncog ...
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... Too frequently it is considered an enemy rather than an essential to normal metabolism. For example, it is a precursor to bile acids, a precursor to steroid hormones, a regulator of cell permeability, an insulator for axons, and perhaps has other important functions. Just why chole terol is found wi ...
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... Thus, Carter said, RNA did not have to invent itself from the primordial soup. Instead, even before there were cells, it seems more likely that there were interactions between amino acids and nucleotides that led to the co-creation of proteins and RNA. Complexity from simplicity Proteins must fold i ...
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... antioxidants  such as urate and ascorbate. Reduced levels of these antioxidants, measured under conditions of oxidative stress, have been related to their reactions with protein radicals. To elucidate whether urate and ascorbate are able to repair protein radicals and whether such a process may be ...
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Lipids and Proteins

... - There are __________ types of amino acids. Our bodies naturally make __________, but we must eat certain foods to obtain the other __________. - Proteins are different from carbs and lipids because they contain the element _________________ in them. They also sometimes contain __________________. ...
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... by weight Proteoglycans are components of the ECM, made up of GAGs, core protein, and link proteins in a complex structure •Hyaluronate forms a backbone onto which large core proteins are attached via linker proteins; other GAGs are attached to core proteins via association with protein-linked glyca ...
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... Nucleotides are composed of carbon, nitrogen, oxygen, phosphorus, and hydrogen. All nucleotides have the three units shown in the figure below—a phosphate, a nitrogenous base, and a sugar. To form a nucleic acid, the sugar of one nucleotide bonds to the phosphate of another nucleotide, as illustrate ...
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... Strep-tag® technology was used from the beginning to purify intact protein complexes in a preparative manner, even if just one subunit carries the tag. The development of a tandem arrangement of two Strep-tag®II sequences, dubbed One-STrEP-tag, improved performance by increasing purification yields ...
Chemistry of Lipids and Proteins
Chemistry of Lipids and Proteins

... c. What is the molecular formula for the amino acid valine? C__H__O__N__ 14. Are the molecular formulas for all amino acids the same? __________________ 15. What end arrangement of atoms is present in amino acids that is also present in fatty acids? ___________________________ Another end arrangeme ...
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... E.g serine family (also include glycine and cysteine) Ultimate precursor of serine is 3-phosphoglycerate (obtainable from glycolitic pathway) The conversion of serine to glycine involves one-C unit from serine to an acceptor This is catalyzed by serine hydroxymethylase, with pyridoxal phosphate as c ...
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... Spinal muscular atrophy (SMA) is a genetic disorder usually leading to death before age two. This is caused by the degeneration of motor neurons in the spine and affects one in six thousand babies yearly (Families of SMA, 2013). It is unknown why a point mutation or deletion of the SMN1 gene, which ...
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Protein structure prediction



Protein structure prediction is the prediction of the three-dimensional structure of a protein from its amino acid sequence — that is, the prediction of its folding and its secondary, tertiary, and quaternary structure from its primary structure. Structure prediction is fundamentally different from the inverse problem of protein design. Protein structure prediction is one of the most important goals pursued by bioinformatics and theoretical chemistry; it is highly important in medicine (for example, in drug design) and biotechnology (for example, in the design of novel enzymes). Every two years, the performance of current methods is assessed in the CASP experiment (Critical Assessment of Techniques for Protein Structure Prediction). A continuous evaluation of protein structure prediction web servers is performed by the community project CAMEO3D.
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