Ileal endogenous amino acid flow response to nitrogen
... alimentation (enzyme hydrolyzed casein) method (Ravindran and Hendriks, 2004), and feeding of highly digestible protein (HDP, Adedokun et al., 2008). Each of these methods has some advantages and disadvantages. Among methods, feeding of NFD may be the preferred method due to its simplicity in method ...
... alimentation (enzyme hydrolyzed casein) method (Ravindran and Hendriks, 2004), and feeding of highly digestible protein (HDP, Adedokun et al., 2008). Each of these methods has some advantages and disadvantages. Among methods, feeding of NFD may be the preferred method due to its simplicity in method ...
Farnesyl diphosphate synthase, the target for nitrogen
... the mammalian mevalonate pathway enzymes remains unclear. The pathway has also been investigated in plants and it appears that some, but not all, of the enzymes are peroxisomal, and that there may also be mitochondrial isoenzymes of both isopentenyl diphosphate isomerase and FDPS [3,11–13]. However, ...
... the mammalian mevalonate pathway enzymes remains unclear. The pathway has also been investigated in plants and it appears that some, but not all, of the enzymes are peroxisomal, and that there may also be mitochondrial isoenzymes of both isopentenyl diphosphate isomerase and FDPS [3,11–13]. However, ...
Document
... conserved in aspartic proteinases, as well as with a modified conserved flap. The complete coding sequences of the mutated clones were obtained and confirmed the desired mutations (Fig 1). Two consecutive phenylalanine residues are present in the flap of Bla g 2. The sequence alignment of Bla g 2 wi ...
... conserved in aspartic proteinases, as well as with a modified conserved flap. The complete coding sequences of the mutated clones were obtained and confirmed the desired mutations (Fig 1). Two consecutive phenylalanine residues are present in the flap of Bla g 2. The sequence alignment of Bla g 2 wi ...
PDF
... not peer-reviewed) is the author/funder. It is made available under a CC-BY 4.0 International license. ...
... not peer-reviewed) is the author/funder. It is made available under a CC-BY 4.0 International license. ...
Holding it all together? Candidate proteins for the plant Golgi matrix
... it is possible to envisage a significant role for proteins of this family in plant Golgi. Conventional BLAST searches of both the Arabidopsis and rice genomes using the mammalian golgins as query sequences detected proteins that have sequence similarity to a subset of the mammalian or yeast golgins ...
... it is possible to envisage a significant role for proteins of this family in plant Golgi. Conventional BLAST searches of both the Arabidopsis and rice genomes using the mammalian golgins as query sequences detected proteins that have sequence similarity to a subset of the mammalian or yeast golgins ...
Computational Studies of Multi-Active Site Enzymes
... binding of its cognate and non-cognate Thr- and Ser-AMP substrates respectively, and (ii) mechanism of hydrolytic pre-transfer editing. In contrast to bound Thr-AMP, bound SerAMP is less constrained; i.e., greater positional variability, and as a result more waters are able to permeate the active si ...
... binding of its cognate and non-cognate Thr- and Ser-AMP substrates respectively, and (ii) mechanism of hydrolytic pre-transfer editing. In contrast to bound Thr-AMP, bound SerAMP is less constrained; i.e., greater positional variability, and as a result more waters are able to permeate the active si ...
Studies on pituitary polypeptide hormones. 2. The action of pepsin
... structure a sequence of seven amino acids, Met. Glu. His. Phe . Arg. Try. Gly, which had previously been shown to occur in the corticotrophins. This common structural feature is in all probability an essential constituent of both melanocyte-stimulating and adrenocorticotrophic hormone molecules, and ...
... structure a sequence of seven amino acids, Met. Glu. His. Phe . Arg. Try. Gly, which had previously been shown to occur in the corticotrophins. This common structural feature is in all probability an essential constituent of both melanocyte-stimulating and adrenocorticotrophic hormone molecules, and ...
Are You suprised ?
... is determined by the sequence of nucleotides in a gene. A change in the DNA nucleotide sequence (mutation) of a gene that codes for a protein may result in a change in the amino-acid sequence of the protein. Biochemical evidence of evolution compares favorably with structural evidence of evolution. ...
... is determined by the sequence of nucleotides in a gene. A change in the DNA nucleotide sequence (mutation) of a gene that codes for a protein may result in a change in the amino-acid sequence of the protein. Biochemical evidence of evolution compares favorably with structural evidence of evolution. ...
Isolation and Characterization of a Novel Ribosome
... second GSP of 29 bp was designed, corresponding to the DNA sequence located approximately 200 bp downstream of the 3⬘-RACE GSPs. 5⬘-RACE was performed and amplification products were cloned and sequenced as described for the 3⬘-RACE procedures. After DNA sequencing of the multiple clones for each am ...
... second GSP of 29 bp was designed, corresponding to the DNA sequence located approximately 200 bp downstream of the 3⬘-RACE GSPs. 5⬘-RACE was performed and amplification products were cloned and sequenced as described for the 3⬘-RACE procedures. After DNA sequencing of the multiple clones for each am ...
Translocation of proteins across the cell envelope of Gram
... Many Gram-negative organisms including E. coli contain a secretion-dedicated chaperone called SecB [27]. SecB functions by rapidly binding to partially folded precursors either free in the cytosol [45] or bound to the ribosome as nascent chains [46]. Next, the binary SecB precursor protein is target ...
... Many Gram-negative organisms including E. coli contain a secretion-dedicated chaperone called SecB [27]. SecB functions by rapidly binding to partially folded precursors either free in the cytosol [45] or bound to the ribosome as nascent chains [46]. Next, the binary SecB precursor protein is target ...
A Personal Account of Chaperonin History
... one could over-express GroEL and GroES and Rubisco in the same E. coli cell. The total amount of Rubisco protein expressed by E. coli was approximately the same whether or not one also over-expressed GroE. What was dramatically different, however, was the fate of Rubisco. In the cells that over-expr ...
... one could over-express GroEL and GroES and Rubisco in the same E. coli cell. The total amount of Rubisco protein expressed by E. coli was approximately the same whether or not one also over-expressed GroE. What was dramatically different, however, was the fate of Rubisco. In the cells that over-expr ...
Journal of Microbiology and Biotechnology
... For quantification of IAA intermediates produced by P. polymyxa E681, indolic compounds in the bacterial supernatant were assayed. Thus, the bacterium was precultured for 20 h in DM medium, and then a 400-µl aliquot was transferred into 100 ml of fresh DM medium supplemented with Trp, obtaining 105 ...
... For quantification of IAA intermediates produced by P. polymyxa E681, indolic compounds in the bacterial supernatant were assayed. Thus, the bacterium was precultured for 20 h in DM medium, and then a 400-µl aliquot was transferred into 100 ml of fresh DM medium supplemented with Trp, obtaining 105 ...
Functional analysis of polar amino
... Several regions of this protein are of particular interest, for example, the membrane-associated segment that does not traverse the membrane is reminiscent of the selectivity filter of potassium channels [24]. These amino acids (387±406) are within a region that is important for amiloride binding [9 ...
... Several regions of this protein are of particular interest, for example, the membrane-associated segment that does not traverse the membrane is reminiscent of the selectivity filter of potassium channels [24]. These amino acids (387±406) are within a region that is important for amiloride binding [9 ...
Poster
... Opsin is a G-protein coupled receptor (GPCR) and an opsin monomer has the typical GPCR structure comprised of seven transmembrane helices which are shown in purple. The beta sheets can be seen in light blue-green. Opsin functions as a dimer. Although retinal was not present in the crystal structure, ...
... Opsin is a G-protein coupled receptor (GPCR) and an opsin monomer has the typical GPCR structure comprised of seven transmembrane helices which are shown in purple. The beta sheets can be seen in light blue-green. Opsin functions as a dimer. Although retinal was not present in the crystal structure, ...
Mutating the second glutamate in the amidase active site
... catalyses the hydrolysis of α-ketoglutaramate (KGM, the α-keto analogue of glutamine) and αketosuccinamate (KSM, the α-keto analogue of asparagine), yielding α-ketoglutarate and oxaloacetate, respectively (1,2). Sequence homology within the nitrilase superfamily identifies the cysteine, glutamate (E ...
... catalyses the hydrolysis of α-ketoglutaramate (KGM, the α-keto analogue of glutamine) and αketosuccinamate (KSM, the α-keto analogue of asparagine), yielding α-ketoglutarate and oxaloacetate, respectively (1,2). Sequence homology within the nitrilase superfamily identifies the cysteine, glutamate (E ...
Towards the construction of Escherichia coli cell
... synthesise the target protein. However, there are some problems when using these systems: the ability to reliably synthesize any biologically active protein in a universal platform, the lack of a cost-effective and scalable platform, and the inability to carry out humanized glycosylation patterns. T ...
... synthesise the target protein. However, there are some problems when using these systems: the ability to reliably synthesize any biologically active protein in a universal platform, the lack of a cost-effective and scalable platform, and the inability to carry out humanized glycosylation patterns. T ...
Multidrug resistance mediated by the ATP-binding
... Sequences were aligned along their entire length with MRP using CLUSTAL W(1.6) multiple sequence alignment. Sequence data were obtained using the following accession numbers: MRP, L05628/P33527; mrp, AF022908/1488428; MOAT, U49248/U63970; EBCR, 1430907/Z49144; C. elegans mrp1, U66260; C. elegans mrp ...
... Sequences were aligned along their entire length with MRP using CLUSTAL W(1.6) multiple sequence alignment. Sequence data were obtained using the following accession numbers: MRP, L05628/P33527; mrp, AF022908/1488428; MOAT, U49248/U63970; EBCR, 1430907/Z49144; C. elegans mrp1, U66260; C. elegans mrp ...
Supplementary Table 1
... Lipid phosphate phosphatase, catalyses Mg(2+)-independent dephosphorylation of phosphatidic acid (PA), lysophosphatidic acid, and diacylglycerol pyrophosphate; involved in control of the cellular levels of phosphatidylinositol and PA ...
... Lipid phosphate phosphatase, catalyses Mg(2+)-independent dephosphorylation of phosphatidic acid (PA), lysophosphatidic acid, and diacylglycerol pyrophosphate; involved in control of the cellular levels of phosphatidylinositol and PA ...
Protein structure prediction
Protein structure prediction is the prediction of the three-dimensional structure of a protein from its amino acid sequence — that is, the prediction of its folding and its secondary, tertiary, and quaternary structure from its primary structure. Structure prediction is fundamentally different from the inverse problem of protein design. Protein structure prediction is one of the most important goals pursued by bioinformatics and theoretical chemistry; it is highly important in medicine (for example, in drug design) and biotechnology (for example, in the design of novel enzymes). Every two years, the performance of current methods is assessed in the CASP experiment (Critical Assessment of Techniques for Protein Structure Prediction). A continuous evaluation of protein structure prediction web servers is performed by the community project CAMEO3D.