Identification and cDNA cloning of a Xenopus nucleolar
... each of these forms has demonstrated that the difference between them is the number of acidic and basic domains at their amino termini. We therefore conclude that the doublet of bands with apparent mass of 95 and 90 kDa that are detected with mAb G1C7 are the two forms of Xenopus nucleolin. This con ...
... each of these forms has demonstrated that the difference between them is the number of acidic and basic domains at their amino termini. We therefore conclude that the doublet of bands with apparent mass of 95 and 90 kDa that are detected with mAb G1C7 are the two forms of Xenopus nucleolin. This con ...
The tyrosine regulated DAHP synthase and the biosynthetic
... the initial reaction of the aromatic amino acid biosynthetic pathway. Phosphoenolpyruvate (PEP) and erythrose-4-phosphate (E4P) are condensed to DAHP. There are two DAHP synthases in baker´s yeast Saccharomyces cerevisiae, encoded by the genes ARO3 and ARO4, that can be feedback-inhibited by phenyla ...
... the initial reaction of the aromatic amino acid biosynthetic pathway. Phosphoenolpyruvate (PEP) and erythrose-4-phosphate (E4P) are condensed to DAHP. There are two DAHP synthases in baker´s yeast Saccharomyces cerevisiae, encoded by the genes ARO3 and ARO4, that can be feedback-inhibited by phenyla ...
The phosphopantetheinyl transferases
... B. brevis, which plays a role in gramicidin S biosynthesis.13 In 1996, a landmark paper co-authored by ve different labs was published. The work comprehensively described the identication of many PPTases using bioinformatics, the characterization of family II proteins by heterologous expression, pu ...
... B. brevis, which plays a role in gramicidin S biosynthesis.13 In 1996, a landmark paper co-authored by ve different labs was published. The work comprehensively described the identication of many PPTases using bioinformatics, the characterization of family II proteins by heterologous expression, pu ...
tRNA
... three-letter amino acid code—eg tRNAArg, tRNAGlu, tRNATrp - What is the difference between tRNAArg and arginyl-tRNA? The tRNAArg is the uncharged (free) tRNA that has the capacity to bind Arg, whereas arginyl-tRNA is the tRNAArg in which Arg is covalently attached to its 3’OH group—strictly, Arginyl ...
... three-letter amino acid code—eg tRNAArg, tRNAGlu, tRNATrp - What is the difference between tRNAArg and arginyl-tRNA? The tRNAArg is the uncharged (free) tRNA that has the capacity to bind Arg, whereas arginyl-tRNA is the tRNAArg in which Arg is covalently attached to its 3’OH group—strictly, Arginyl ...
Purification and Characterization of Bacteriocin Produced by Lactococcus lactis
... characterized.The highly promising results of these studies underline the important role that functional, bacteriocinogenic LAB strains may play in the food industry as starter cultures, co-cultures, or bioprotective cultures, to improve food quality and safety [4 ]. Lactococcus lactis strains are w ...
... characterized.The highly promising results of these studies underline the important role that functional, bacteriocinogenic LAB strains may play in the food industry as starter cultures, co-cultures, or bioprotective cultures, to improve food quality and safety [4 ]. Lactococcus lactis strains are w ...
Microtubules
... as well as other proteins, are either present in centrioles or required for their formation. ...
... as well as other proteins, are either present in centrioles or required for their formation. ...
FEBS Letters 541, 176-177
... rate K when Hes1 is bound to the DNA. The probability that Hes1 is bound to DNA is kh /(kh +[Hes1]h ), where k is a characteristic concentration for dissociation of Hes1 from the DNA, and h is the Hill coe⁄cient that takes into account the cooperative character of the binding process. Moreover, Eq. ...
... rate K when Hes1 is bound to the DNA. The probability that Hes1 is bound to DNA is kh /(kh +[Hes1]h ), where k is a characteristic concentration for dissociation of Hes1 from the DNA, and h is the Hill coe⁄cient that takes into account the cooperative character of the binding process. Moreover, Eq. ...
Molecular Characterisation of the 76 kDa Iron
... Genes encoding subunits of complex I (EC 1.6.5.3) of the mitochondrial respiratory chain vary in their locations between the mitochondrial and nuclear genomes in different organisms, whereas genes for a homologous multisubunit complex in chloroplasts have to date only been found on the plastid genom ...
... Genes encoding subunits of complex I (EC 1.6.5.3) of the mitochondrial respiratory chain vary in their locations between the mitochondrial and nuclear genomes in different organisms, whereas genes for a homologous multisubunit complex in chloroplasts have to date only been found on the plastid genom ...
Using Mascot to characterise protein modifications
... This slide shows three peptides with the same parent mass, but they differ in the PTM site(s). There are 4 Lysines that can be modified, there is the amino terminus and a Ser and an Arg residue. The possible combination of PTM sites is large. The bottom spectrum demonstrates how four different PTMs ...
... This slide shows three peptides with the same parent mass, but they differ in the PTM site(s). There are 4 Lysines that can be modified, there is the amino terminus and a Ser and an Arg residue. The possible combination of PTM sites is large. The bottom spectrum demonstrates how four different PTMs ...
Johansson L, Gafvelin G, Arnér ES. Selenocysteine in proteins
... to accept a proton and subsequently transfer it to a nearby His residue, in close proximity with the Mo-atom. Formate dehydrogenase seems to be the most common selenoprotein in bacteria [42]. Another bacterial selenoprotein found in some Grampositive anaerobes is glycine reductase, which is a comple ...
... to accept a proton and subsequently transfer it to a nearby His residue, in close proximity with the Mo-atom. Formate dehydrogenase seems to be the most common selenoprotein in bacteria [42]. Another bacterial selenoprotein found in some Grampositive anaerobes is glycine reductase, which is a comple ...
Titration curves of proteins
... Thus, in proteins the local environment of titratable groups is much more complex due to the interactions between titratable groups and other partial charges of polar groups as well as ions in the surrounding solvent. Finally, the dielectric environment is changing when the group is transferred from ...
... Thus, in proteins the local environment of titratable groups is much more complex due to the interactions between titratable groups and other partial charges of polar groups as well as ions in the surrounding solvent. Finally, the dielectric environment is changing when the group is transferred from ...
Extreme sweetness: protein glycosylation in archaea
... *X represents any amino acid. ‡X represents any amino acid except Pro. ...
... *X represents any amino acid. ‡X represents any amino acid except Pro. ...
Isolation of Rat Transferrin Using CNBr-Activated
... four separate analyses of each of the fractions, and from the eight values of both fractions together. ...
... four separate analyses of each of the fractions, and from the eight values of both fractions together. ...
Journal of Agricultural and Food Chemistry 48
... molecular weights. Previous papers have suggested that molecular heterogeneity within various purified PNA preparations (Miller, 1983) is basically due to the dissimilar composition of the isolectins' subunits. Although each subunit has essentially the same characteristic tertiary fold that is found ...
... molecular weights. Previous papers have suggested that molecular heterogeneity within various purified PNA preparations (Miller, 1983) is basically due to the dissimilar composition of the isolectins' subunits. Although each subunit has essentially the same characteristic tertiary fold that is found ...
Transmembrane Fragment Structures of Amyloid Precursor Protein
... TM helix, accords well with experimental observations. Based on our simulations, G29-L52 distances in the range of 33−34 Å are inconsistent with the presence of a structural kink in the TM helix near G37/G38. Consistent with this view, the G29-L52 distances derived from the PDB structures (orange li ...
... TM helix, accords well with experimental observations. Based on our simulations, G29-L52 distances in the range of 33−34 Å are inconsistent with the presence of a structural kink in the TM helix near G37/G38. Consistent with this view, the G29-L52 distances derived from the PDB structures (orange li ...
Modulation of the Oligomerization State of the Bovine F1
... Construction of Bacterial Expression Plasmids—The expression plasmid pRKIF1 contains the coding sequence for the entire bovine IF1 (11). Plasmids expressing IF1-H49K and IF1 truncated at the N terminus by 31 residues [IF1 (residues 32– 84)] were made from pRKIF1 by polymerase chain reaction using th ...
... Construction of Bacterial Expression Plasmids—The expression plasmid pRKIF1 contains the coding sequence for the entire bovine IF1 (11). Plasmids expressing IF1-H49K and IF1 truncated at the N terminus by 31 residues [IF1 (residues 32– 84)] were made from pRKIF1 by polymerase chain reaction using th ...
Ube2W conjugates ubiquitin to α-amino groups of protein N
... (Pep.6His-SUMO-2 × 4 ) (Figure 1A, right-hand side) was used as a substrate in the assay, which can be bound by RNF4 in a manner similar to the native isopeptide bond-linked polySUMO chain (Isopep.6His-SUMO-2 × 4 ) (Figure 1A, left-hand side). The screen showed that, along with the Ubc4/Ubc5 family, ...
... (Pep.6His-SUMO-2 × 4 ) (Figure 1A, right-hand side) was used as a substrate in the assay, which can be bound by RNF4 in a manner similar to the native isopeptide bond-linked polySUMO chain (Isopep.6His-SUMO-2 × 4 ) (Figure 1A, left-hand side). The screen showed that, along with the Ubc4/Ubc5 family, ...
Protein structure prediction
Protein structure prediction is the prediction of the three-dimensional structure of a protein from its amino acid sequence — that is, the prediction of its folding and its secondary, tertiary, and quaternary structure from its primary structure. Structure prediction is fundamentally different from the inverse problem of protein design. Protein structure prediction is one of the most important goals pursued by bioinformatics and theoretical chemistry; it is highly important in medicine (for example, in drug design) and biotechnology (for example, in the design of novel enzymes). Every two years, the performance of current methods is assessed in the CASP experiment (Critical Assessment of Techniques for Protein Structure Prediction). A continuous evaluation of protein structure prediction web servers is performed by the community project CAMEO3D.