Dual-topology membrane proteins Escherichia coli Susanna Seppälä
... Cellular life, as we know it, is absolutely dependent on biological membranes; remarkable superstructures made of lipids and proteins. For example, all living cells are surrounded by at least one membrane that protects the cell and holds it together. The proteins that are embedded in the membranes c ...
... Cellular life, as we know it, is absolutely dependent on biological membranes; remarkable superstructures made of lipids and proteins. For example, all living cells are surrounded by at least one membrane that protects the cell and holds it together. The proteins that are embedded in the membranes c ...
Multiple Alignment
... MSA applies both to nucleotide and amino acid sequences To construct a multiple alignment, one may have to introduce gaps in sequences at positions where there were no gaps in the corresponding pairwise alignment. This means that multiple alignments typically contain more gaps than any given pair of ...
... MSA applies both to nucleotide and amino acid sequences To construct a multiple alignment, one may have to introduce gaps in sequences at positions where there were no gaps in the corresponding pairwise alignment. This means that multiple alignments typically contain more gaps than any given pair of ...
Mechanistic Studies Of Drug Resistance Conferred By An ABC
... Fig. 1.1 Schematic representation of the conserved motifs in DrrA…………………………….43 Fig. 1.2 Disulfide cross-linking between S319C in DrrA and S23C or C260 in DrrB………….45 Fig. 1.3 Disulfide cross-linking between T311C, S302C, S287C, S253C, or S232C in DrrA and S23C in DrrB… ............................. ...
... Fig. 1.1 Schematic representation of the conserved motifs in DrrA…………………………….43 Fig. 1.2 Disulfide cross-linking between S319C in DrrA and S23C or C260 in DrrB………….45 Fig. 1.3 Disulfide cross-linking between T311C, S302C, S287C, S253C, or S232C in DrrA and S23C in DrrB… ............................. ...
Regulation of Translation by Essential Amino Acids and Glucose in
... Amino Acid Nutrition Essential amino acids and N must be absorbed in sufficient quantities from the gastrointestinal tract to support the synthesis of milk protein and the maintenance of body tissues in the lactating cow. Dietary protein can be classified as either rumen-degradable (RDP) or rumen-un ...
... Amino Acid Nutrition Essential amino acids and N must be absorbed in sufficient quantities from the gastrointestinal tract to support the synthesis of milk protein and the maintenance of body tissues in the lactating cow. Dietary protein can be classified as either rumen-degradable (RDP) or rumen-un ...
Identification of Bioactive Peptide Sequences from Amaranth
... From the whole grain, tasteful soups, stews, sauces, porridges, and soufflés can be prepared. Boiled grains can be used like rice and couscous, which is traditionally made with semolina of wheat. When amaranth grains are boiled, the starch is leaching out and is gelatinized. This causes the cooking ...
... From the whole grain, tasteful soups, stews, sauces, porridges, and soufflés can be prepared. Boiled grains can be used like rice and couscous, which is traditionally made with semolina of wheat. When amaranth grains are boiled, the starch is leaching out and is gelatinized. This causes the cooking ...
biochemical, biophysical and electrophysiological characterisation
... The eukaryotic voltage-gated sodium channel Na v is an integral membrane protein that transports sodium across the cell membrane. While the Na v has a four-domain structure with six α-helical transmembrane segments in each domain, the first bacterial homologue, which was identified in 2001 in Bacill ...
... The eukaryotic voltage-gated sodium channel Na v is an integral membrane protein that transports sodium across the cell membrane. While the Na v has a four-domain structure with six α-helical transmembrane segments in each domain, the first bacterial homologue, which was identified in 2001 in Bacill ...
A 5000-Fold Increase in the Specificity of a Bacterial
... insecticide malathion is lower than expected based on its physical properties; principally the pka of its leaving group. In this study, we rationalise the turnover rate of PTEAr for malathion using computational docking of the substrate into a high resolution crystal structure of the enzyme, suggest ...
... insecticide malathion is lower than expected based on its physical properties; principally the pka of its leaving group. In this study, we rationalise the turnover rate of PTEAr for malathion using computational docking of the substrate into a high resolution crystal structure of the enzyme, suggest ...
The nullo protein is a component of the actin
... 1990). Like its transcript, the sry-α protein is short-lived. The nullo gene is predicted to encode a 23 kDa protein lacking homology to known proteins, including the sry-α protein. Sequence analysis demonstrated that the nullo protein has an excess of basic amino acids (predicted pI is 11.4) and su ...
... 1990). Like its transcript, the sry-α protein is short-lived. The nullo gene is predicted to encode a 23 kDa protein lacking homology to known proteins, including the sry-α protein. Sequence analysis demonstrated that the nullo protein has an excess of basic amino acids (predicted pI is 11.4) and su ...
Full-Text PDF
... A member of another class of PR proteins, defensin protein PDF1.2, tagged with green fluoresecent protein (GFP) and overexpressed in Arabidopsis, localizes in the endoplasmic reticulum (ER)-derived structures called ER bodies [10–12]. However, after a fungal attack in the presence of glucose which i ...
... A member of another class of PR proteins, defensin protein PDF1.2, tagged with green fluoresecent protein (GFP) and overexpressed in Arabidopsis, localizes in the endoplasmic reticulum (ER)-derived structures called ER bodies [10–12]. However, after a fungal attack in the presence of glucose which i ...
Use of a Sec signal peptide library from Bacillus subtilis for the
... SP performance with respect to the secretory production of cutinase using C. glutamicum. To allow a direct comparison of the results for SP impact on cutinase secretion with B. subtilis obtained by Brockmeier et al. [16], four B. subtilis expression strains with different signal peptides (YwmC, A ...
... SP performance with respect to the secretory production of cutinase using C. glutamicum. To allow a direct comparison of the results for SP impact on cutinase secretion with B. subtilis obtained by Brockmeier et al. [16], four B. subtilis expression strains with different signal peptides (YwmC, A ...
A model for mis-sense error in protein synthesis: mis
... is also the correct one, as directed by the corresponding template; the other end of the same cognate tRNA molecule, referred to as anti-codon, matches perfectly, by complementary base pairing, with the codon on the template mRNA. In contrast, increasing degree of mismatch makes the tRNA near-cognat ...
... is also the correct one, as directed by the corresponding template; the other end of the same cognate tRNA molecule, referred to as anti-codon, matches perfectly, by complementary base pairing, with the codon on the template mRNA. In contrast, increasing degree of mismatch makes the tRNA near-cognat ...
12 Insights into the mechanisms underlying CFTR channel activity
... In addition to the structural features shared with other ABC proteins, CFTR also contains a unique 241‑residue R domain in the linker region that joins the two halves of the protein [35] and is essential for its activity [36]. The highly charged R domain possesses multiple phosphorylation sites for ...
... In addition to the structural features shared with other ABC proteins, CFTR also contains a unique 241‑residue R domain in the linker region that joins the two halves of the protein [35] and is essential for its activity [36]. The highly charged R domain possesses multiple phosphorylation sites for ...
AMINO ACID OXIDATION AND THE PRODUCTION OF UREA
... 3. During starvation or in uncontrolled diabetes mellitus, when carbohydrates are either unavailable or not properly utilized, cellular proteins are used as fuel. Under all these metabolic conditions, amino acids lose their amino groups to form -keto acids, the “carbon skeletons” of amino acids. Th ...
... 3. During starvation or in uncontrolled diabetes mellitus, when carbohydrates are either unavailable or not properly utilized, cellular proteins are used as fuel. Under all these metabolic conditions, amino acids lose their amino groups to form -keto acids, the “carbon skeletons” of amino acids. Th ...
Міністерство охорони здоров`я України Харківський
... metabolism of basic classes of biomolecules (proteins, amino acids, nucleic acids, nucleotides, carbohydrates, lipids), regulation of metabolism and physiologic functions, biochemistry of enzymes, vitamins, blood, kidney, muscle, liver, immune, nervous, connective tissues. Modern conceptions of mole ...
... metabolism of basic classes of biomolecules (proteins, amino acids, nucleic acids, nucleotides, carbohydrates, lipids), regulation of metabolism and physiologic functions, biochemistry of enzymes, vitamins, blood, kidney, muscle, liver, immune, nervous, connective tissues. Modern conceptions of mole ...
Critically discuss the methods that are in routine use for the
... binding is usually measured. However, dye binding methods are less specific than nephelometry or turbidimetry and are inaccurate if the overall serum protein pattern is abnormal, such as in multiple myeloma. The BCG and BCP methods both have advantages and disadvantages, with neither being clearly p ...
... binding is usually measured. However, dye binding methods are less specific than nephelometry or turbidimetry and are inaccurate if the overall serum protein pattern is abnormal, such as in multiple myeloma. The BCG and BCP methods both have advantages and disadvantages, with neither being clearly p ...
INTEINS: Structure, Function, and Evolution
... contacts using residues from the endonuclease domain (93). However, a part of the other domain that is distant from the PI-SceI cleavage site also contributes to the recognition of the target sequence (24, 43). These additional interactions were determined using photo-crosslinking and affinity cleav ...
... contacts using residues from the endonuclease domain (93). However, a part of the other domain that is distant from the PI-SceI cleavage site also contributes to the recognition of the target sequence (24, 43). These additional interactions were determined using photo-crosslinking and affinity cleav ...
Uptake and assimilation of nitrogen from solutions containing
... Hempstead, UK). The uptake of the 15N labelled compounds was determined using the equations of Millard & Nielsen (1989). From the observed rates of uptake (see Results) it was estimated that depletion of any individual form of N from the uptake solution ranged from 7% (nitrate in the mixed nutrient ...
... Hempstead, UK). The uptake of the 15N labelled compounds was determined using the equations of Millard & Nielsen (1989). From the observed rates of uptake (see Results) it was estimated that depletion of any individual form of N from the uptake solution ranged from 7% (nitrate in the mixed nutrient ...
Insights into interactions between poly(ethylene glycol) and proteins
... inertness with protein surfaces8-9. Dextran is a mostly linear polymer composed of glucose monomers linked via α-(1,6)-D-glycosidic bonds (approximately 95%) with an occasional α-(1,3)-D-glycosidic linkage10-12. ...
... inertness with protein surfaces8-9. Dextran is a mostly linear polymer composed of glucose monomers linked via α-(1,6)-D-glycosidic bonds (approximately 95%) with an occasional α-(1,3)-D-glycosidic linkage10-12. ...
148 - Blue Ridge Institute for Medical Research
... Protein kinases play a predominant regulatory role in nearly every aspect of cell biology and they can modify the function of a protein in almost every conceivable way. Protein phosphorylation can increase or decrease enzyme activity and it can alter other biological activities such as transcription ...
... Protein kinases play a predominant regulatory role in nearly every aspect of cell biology and they can modify the function of a protein in almost every conceivable way. Protein phosphorylation can increase or decrease enzyme activity and it can alter other biological activities such as transcription ...
Protein structure prediction
Protein structure prediction is the prediction of the three-dimensional structure of a protein from its amino acid sequence — that is, the prediction of its folding and its secondary, tertiary, and quaternary structure from its primary structure. Structure prediction is fundamentally different from the inverse problem of protein design. Protein structure prediction is one of the most important goals pursued by bioinformatics and theoretical chemistry; it is highly important in medicine (for example, in drug design) and biotechnology (for example, in the design of novel enzymes). Every two years, the performance of current methods is assessed in the CASP experiment (Critical Assessment of Techniques for Protein Structure Prediction). A continuous evaluation of protein structure prediction web servers is performed by the community project CAMEO3D.