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SRF - Journal of Cell Science
SRF - Journal of Cell Science

... Serum response factor (SRF) is a ubiquitous transcription factor that binds to the DNA sequence CC(A/T)6GG, which has been identified as an essential regulatory serum response element (SRE) of the c-fos proto-oncogene promoter (for reviews see Rivera and Greenberg, 1990; Treisman, 1990, 1992; Piecha ...
Altering protein specificity: techniques and applications
Altering protein specificity: techniques and applications

The Plasma Membrane of Arabidopsis thaliana Contains a Mercury
The Plasma Membrane of Arabidopsis thaliana Contains a Mercury

... a limited osmotic water flux through the oocyte plasma membrane, whose permeability can be accounted for mainly by lipid-mediated water transport (Zhang and Verkman, 1991). In contrast, oocytes injected with rd28 cRNA or y-tip cRNA rapidly increased their volume by up to 40% (Fig. 2A) and ruptured i ...
Restricted rotation of the amino group of nucleic acid base
Restricted rotation of the amino group of nucleic acid base

Protein expression in pectoral skeletal muscle of chickens as
Protein expression in pectoral skeletal muscle of chickens as

... nutrient recommendations (NRC, 1994), was fed to all the broilers in the study from placement until 21 d of age (Table 1). Subsequently, the test diet was fed from 21 to 42 d of age (Table 1) and was formulated to satisfy all nutrient recommendations for the 21- to 42-d period, with the exception of ...
The Reactions of Diazonium Compounds with Amino Acids and
The Reactions of Diazonium Compounds with Amino Acids and

COMBINING MOLECULAR DOCKING WITH RECEPTOR DOMAIN
COMBINING MOLECULAR DOCKING WITH RECEPTOR DOMAIN

... 1EUL (“open” apo-form), 1IWO (another “open” apo-form), and 1T5S (“closed” holo-form). The GROMACS program and the GROMOS96 force field (Berendsen et al., 1995) were used. Molecules with uncharged N- and C-termini were placed in rectangular boxes of a simple point charge (SPC) model of water molecul ...
Coupling Coherence Distinguishes Structure Sensitivity in Protein
Coupling Coherence Distinguishes Structure Sensitivity in Protein

... We suggest that, in the multiple-pathway regime, the evolutionary linkage between the specific protein fold and the ET rate is likely to be weak: In this regime, RDA determines tunneling propensity. In the single-pathway large C regime, however, ET kinetics and protein structure are strongly linked. ...
Breakfast Computations Simplified
Breakfast Computations Simplified

... Microwave energy from the sun and other stars is direct current based. Artificially produced microwaves, including those in ovens, are produced from alternating current and force a billion or more polarity reversals per second in the water molecules of every food molecule they hit. Production of unn ...
MSc in Biochemistry Dissertation Project – 2nd Cycle Student´s
MSc in Biochemistry Dissertation Project – 2nd Cycle Student´s

... Glycosylation is the most complex and widespread process of posttranslational modification of proteins and lipids, with an unsurpassed capacity to generate a wide array of structures (Annu. Rev. Biomed. Eng. 2007, 9, 121–167). The large polypeptide GalNAc-transferase (GalNAc-Ts) family catalyzes the ...
Amino acid sequence of rabbit ventricular myosin light chain
Amino acid sequence of rabbit ventricular myosin light chain

... about 17 years ago (Lowey et al., 1969). In recent years with the development of new techniques for directly isolating and characterizing the light chain genes themselves, this interest has intensified (for recent review, see Barton and Buckingham, 1985). The sequences around the thiol groups of rab ...
The Maize Abscisic Acid-Responsive Protein Rabl7
The Maize Abscisic Acid-Responsive Protein Rabl7

... nitrocellulose filters and the wild-type peptide conjugated to human serum albumin (HSA) or the 14C-labeled free wild-type peptide in solution. Binding was detected by antibodies against HSA or, in the case of 14C-labeled free wild-type NLS peptide, by autoradiography. Figure 6A shows the interactio ...
1. Products of Amino Acid Transamination Name
1. Products of Amino Acid Transamination Name

... amino acids needed for protein synthesis and the distribution of amino acids in the dietary protein. Gelatin contains a nutritionally unbalanced distribution of amino acids. As large amounts of gelatin are ingested and the excess amino acids are catabolized, the capacity of the urea cycle may be exc ...
SAPS(1) USER COMMANDS SAPS(1) NAME saps
SAPS(1) USER COMMANDS SAPS(1) NAME saps

... protein sequence properties. Properties considered include compositional biases; clusters and runs of charge and other amino acid types; different kinds and extents of repetitive structures; locally periodic motifs; and anomalous spacings between identical residue types. The statistics are computed ...
BLAST Phase 1 - Central Web Server 2
BLAST Phase 1 - Central Web Server 2

... STS - Sequence Tagged Sites; short genomic markers for mapping Swissprot - well-annotated amino-acid sequences ...
`Don`t talk to me about permeability`
`Don`t talk to me about permeability`

... it was first put forward. Better techniques of study have shown that membrane proteins do not, in general, exist in extended form but are largely in helical conformation, that the lipid core is in a disordered rather than highly orientated state, and that hydrophobic sections of protein penetrate th ...
Module Number- 3181
Module Number- 3181

... Written evidence of the ability to describe the process of replication, transcription and translation and provide representations of the structure of DNA and RNA. An appropriate performance level must be used when judging sufficiency of evidence. ...
Breakfast Computations Simplified
Breakfast Computations Simplified

... Microwave energy from the sun and other stars is direct current based. Artificially produced microwaves, including those in ovens, are produced from alternating current and force a billion or more polarity reversals per second in the water molecules of every food molecule they hit. Production of unn ...
8.5 Translation - Cloudfront.net
8.5 Translation - Cloudfront.net

... – Messenger RNA (mRNA) which was transcribed from DNA, carries the message that will be translated to form a protein. – Ribosomal RNA (rRNA) forms part of ribosomes where proteins are made. – Transfer RNA (tRNA) brings amino acids from the cytoplasm to a ribosome. ...
Protein conformational changes induced by adsorption onto material
Protein conformational changes induced by adsorption onto material

... which result from the replacement of protein-solvent interactions by protein-material interactions. This simple balance between the internal and external interactions was proposed by Norde to relate the protein behavior in contact with the material to their stability in solution: “flexible” proteins ...
FoldSynth: Interactive 2D/3D Visualisation Platform for Molecular
FoldSynth: Interactive 2D/3D Visualisation Platform for Molecular

... The particles and springs for a FoldSynth model may be set up in various ways. The most common way is to load a known molecular strand, e.g. a protein from a central resource such as the Protein DataBank (PDB) [B∗ 00], either as a saved file or by web lookup. Alternatively, a user may type in an ami ...
Common Structural Domains in the Sarcoplasmic Reticulum Ca
Common Structural Domains in the Sarcoplasmic Reticulum Ca

SDS-PAGE strongly overestimates the molecular
SDS-PAGE strongly overestimates the molecular

... Gel filtration was on Sephacryl S-400 and S-300 (Pharmacia). Protein elution was monitored by trichloroacetic acid precipitation or SDS-PAGE. Alternatively proteins were labelled either by rz51 or by the fluorescent reagent fluoresceine isothiocyanate [9]. Standards were from Boehringer with the exc ...
Shine – Dalgarno sequence
Shine – Dalgarno sequence

... factors IF2-GTP, IF1, IF3, as well as the initiator tRNA fMet-tRNA (fMet) are recruited to the ribosome. In Gram-negative bacteria, however, Shine-Dalgarno sequence presence is not obligatory for ribosome to locate initiator codon, since deletion of Anti-Shine-Dalgarno sequence from 16S rRNA doesn't ...
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Protein structure prediction



Protein structure prediction is the prediction of the three-dimensional structure of a protein from its amino acid sequence — that is, the prediction of its folding and its secondary, tertiary, and quaternary structure from its primary structure. Structure prediction is fundamentally different from the inverse problem of protein design. Protein structure prediction is one of the most important goals pursued by bioinformatics and theoretical chemistry; it is highly important in medicine (for example, in drug design) and biotechnology (for example, in the design of novel enzymes). Every two years, the performance of current methods is assessed in the CASP experiment (Critical Assessment of Techniques for Protein Structure Prediction). A continuous evaluation of protein structure prediction web servers is performed by the community project CAMEO3D.
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