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Mitochondria are the - Charlin Manchester Terriers
Mitochondria are the - Charlin Manchester Terriers

... contribute the same number of genes from their own DNA during mitosis, and those genes match up and form the new DNA helixes in each puppy, right? So the genetic influence from each parent must be exactly 50/50, right? Well, yes – when you're talking about nuclear DNA. However, there is the mitochon ...
week9_DNA&geneExpression.bak
week9_DNA&geneExpression.bak

... DNA & Genetic Code • The genetic code is both universal and degenerate. – Universal = found in all living organisms – Degenerate = having more than one base triplet (codon) to code for one amino acid ...
Locked Nucleic Acid - LNA™
Locked Nucleic Acid - LNA™

... methylene bridge connecting the 2’-O atom with the 4’-C atom (see structure below). LNA™ nucleosides contain the six common nucleobases (T, C, G, A, U and mC) that appear in DNA and RNA and thus are able to form base-pairs according to standard Watson-Crick base pairing rules. Oligonucleotides incor ...
Epigenetics Glossary FINAL
Epigenetics Glossary FINAL

... CpG island shores: Regions of DNA that lie at the outskirts of CpG islands rather than in the islands itself. They have variable methylation and have been proposed to be enriched in methylation differences between tissue types or between normal and cancer samples. CpG island: Regions of DNA enriched ...
lec36_2013 - Andrew.cmu.edu
lec36_2013 - Andrew.cmu.edu

... 1. Utilized a template to direct the order of added bases. 2. DNA polymerases require a basepaired primer with a 3’OH, RNA poly. do not. 3. Synthesize chains from 5'3' direction. Adding new bases to the 3' hydroxyl of the existing polymer. 4. Use nucleotside triphosphates, producing a chain one bas ...
Genetic Engineering Notes
Genetic Engineering Notes

... Stem cells are extracted from the egg after it has divided for 5 days. ...
Strawberry DNA Extraction Lab
Strawberry DNA Extraction Lab

... bilayers of the cell membrane and organelles. The salt is used to break up protein chains that bind around the nucleic acids. Finally, ethanol is added. DNA is soluble in water, but not ethanol. The colder the ethanol, the less soluble the DNA will be. The alcohol causes DNA to precipitate, or settl ...
Homework 1
Homework 1

Section 8.7 Mutations
Section 8.7 Mutations

... 2. Mutagens – Environmental factors that cause changes in DNA Examples: - Radiation - Chemicals in environment ...
lec-09-forensic-dna-analysis-chem-195h-2017
lec-09-forensic-dna-analysis-chem-195h-2017

... degrees. Now, an enzyme called Taq DNA polymerase is added. This is a very stable enzyme isolated from bacteria living at thermal vents in the ocean (up to 95 oC) In just 32 rounds of PCR, 1 copy of DNA becomes 4.2 billion copies. This would take about 3 hours to perform in lab. ...
Chapter 20 DNA Technology
Chapter 20 DNA Technology

... • Reverse transcriptase enzymes can take n “edited” message and change it into a gene GENE CLONING in BACTERIA •process used to produce multiple copies of specific segments of DNA • Isolate bacterial plasmid and foreign DNA •Treating with same restriction enzyme produces same “sticky ends” • Mix DNA ...
DNA
DNA

... The synthesis of specific protein under the direction of specific gene is complex. Proteins are the polymer of 20 different amino acids and there are only four different nucleotide monomers in DNA. Hence, there can not be a one-to-one relationship between the sequence of nucleotides in the DNA molec ...
Chapter 10 – DNA Replication
Chapter 10 – DNA Replication

... initiate a new strand – it can only elongate an existing strand – Primase • RNA polymerase • Does not require a primer • Adds short stretch of RNA nucleotides which is later replaced by DNA nucleotides and ligated together ...
PUTTING DNA to WORK: High School Virtual Field Trip
PUTTING DNA to WORK: High School Virtual Field Trip

... Billion Random Letters ...
DNA BASE PAIR “Friendship Bracelets” Background: DNA is the
DNA BASE PAIR “Friendship Bracelets” Background: DNA is the

... the nucleus, where it is changed into RNA. In prokaryotic cells (like the bacteria syphilis from our movie), it is found twisted in the center. In DNA, there are latter-like structures, connected in the middle by nitrogenous bases that always make a pair. As you can see, “A” adenine always links up ...
MICROBIAL GENETICS
MICROBIAL GENETICS

... • Use of DNA polymerase to make a large number of copies of a DNA template in vitro • One DNA template --> billions of copies within a few hours • Process involves alternating cycles of heat denaturation and ...
Chapter 15 - Dr. Jennifer Capers
Chapter 15 - Dr. Jennifer Capers

... specify each amino acid • Stop codons – 3 codons (UUA, UGA, UAG) used to terminate translation ...
Lecture 2 - Websupport1
Lecture 2 - Websupport1

... carbohydrates, lipids, proteins, nucleic acids and high energy compounds. ...
Evolution - Issaquah Connect
Evolution - Issaquah Connect

... o Replication vs Transcription  Replication creates two, ________ of __NA  Transcription replicates specific ______ and creates ___NA, can create _____ copy/ copies o Replication: occurs within ________, resulting _____ remains in nucleus o Transcription: occurs within ________, resulting _____ le ...
ap® biology 2009 scoring guidelines - AP Central
ap® biology 2009 scoring guidelines - AP Central

File
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... Remember- ** There are two strands to DNA- The strand that is synthesized in the 5' to 3' direction is called the leading strand and is described below: First the enzyme, helicase unwinds the two strands of DNA. Once the strands are separated, a replication fork is established. As the helicase unwin ...
bioblankspdfver - Issaquah Connect
bioblankspdfver - Issaquah Connect

... o Replication vs Transcription  Replication creates two, ________ of __NA  Transcription replicates specific ______ and creates ___NA, can create _____ copy/ copies o Replication: occurs within ________, resulting _____ remains in nucleus o Transcription: occurs within ________, resulting _____ le ...
Horizontal Gene Transfer
Horizontal Gene Transfer

... Transduction: bacterial DNA transferred by viruses (phage) Conjugation: DNA transfer between bacterial cells ...
2421_Ch9.ppt
2421_Ch9.ppt

... Gene Libraries - collection of clones containing different DNA fragments (each one a part of the genome) cDNA (complementary DNA) - eukaryotic genes cannot be easily cloned in bacteria due to the presence of introns (stretches of DNA inside a gene which do not code for protein -- the coding parts ar ...
DNA
DNA

... DNA strand after it has separated • Topoisomerase: Relieves the “overwinding” strain that can occur ahead of the replication fork (swivel motion) • Primase: Synthesizes an RNA primer at the 5’ end of replicating strand. • DNA pol III: Using the template strand, covalently bonds nucleotides to the 3’ ...
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Nucleic acid analogue



Nucleic acid analogues are compounds which are analogous (structurally similar) to naturally occurring RNA and DNA, used in medicine and in molecular biology research.Nucleic acids are chains of nucleotides, which are composed of three parts: a phosphate backbone, a pucker-shaped pentose sugar, either ribose or deoxyribose, and one of four nucleobases.An analogue may have any of these altered. Typically the analogue nucleobases confer, among other things, different base pairing and base stacking properties. Examples include universal bases, which can pair with all four canonical bases, and phosphate-sugar backbone analogues such as PNA, which affect the properties of the chain (PNA can even form a triple helix).Nucleic acid analogues are also called Xeno Nucleic Acid and represent one of the main pillars of xenobiology, the design of new-to-nature forms of life based on alternative biochemistries.Artificial nucleic acids include peptide nucleic acid (PNA), Morpholino and locked nucleic acid (LNA), as well as glycol nucleic acid (GNA) and threose nucleic acid (TNA). Each of these is distinguished from naturally occurring DNA or RNA by changes to the backbone of the molecule.In May 2014, researchers announced that they had successfully introduced two new artificial nucleotides into bacterial DNA, and by including individual artificial nucleotides in the culture media, were able to passage the bacteria 24 times; they did not create mRNA or proteins able to use the artificial nucleotides. The artificial nucleotides featured 2 fused aromatic rings.
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