STAAR Review 3
... which is caused by recessive alleles. The other parent does not have the PKU alleles. What is the chance that the couple will have a child with phenylketonuria? a. O% b. 50% c. 75% d. 100% ...
... which is caused by recessive alleles. The other parent does not have the PKU alleles. What is the chance that the couple will have a child with phenylketonuria? a. O% b. 50% c. 75% d. 100% ...
Plasmids, primers (and beyond!)
... Cohesive ends can be formed on a DNA fragment (e.g., a synthetic oligonucleotide) by adding a short segment using a DNA ligase (such as the one from T4 ligase). The short segment contains a cleavage site for a restriction enzyme, in this case, EcoR1. EcoR1 forms an overhanging adhesive site. ...
... Cohesive ends can be formed on a DNA fragment (e.g., a synthetic oligonucleotide) by adding a short segment using a DNA ligase (such as the one from T4 ligase). The short segment contains a cleavage site for a restriction enzyme, in this case, EcoR1. EcoR1 forms an overhanging adhesive site. ...
Enzyme Mechanisms - Illinois Institute of Technology
... Boundary condition is fully denatured concentration c0 at time t=0: c / c0 = (1+k2c0t)-1 Half time is t1/2 = (k2c0)-1 Routine depiction: plot c0t vs. fraction reassociated (c /c0) and find the halfway ...
... Boundary condition is fully denatured concentration c0 at time t=0: c / c0 = (1+k2c0t)-1 Half time is t1/2 = (k2c0)-1 Routine depiction: plot c0t vs. fraction reassociated (c /c0) and find the halfway ...
Genetics Exam 5
... Problems (3 points each) You want to design an oligonucleotide probe to identify a clone containing a new enzyme that you purified. You determine that the amino terminal sequence of your enzyme is: MCFYMDW What should be the sequence of the oligonucleotide probe? Indicate redundancy by putting all ...
... Problems (3 points each) You want to design an oligonucleotide probe to identify a clone containing a new enzyme that you purified. You determine that the amino terminal sequence of your enzyme is: MCFYMDW What should be the sequence of the oligonucleotide probe? Indicate redundancy by putting all ...
Diapositive 1 - ac-aix
... material in all the alive beings? We wants to extract from the DNA of different cellular species such as: - Plant species : the onion - Animal species eukaryote* pluricellular : saliva - Animal species eukaryote* pluricellular : liver of mouse - Animal species prokaryote** : bacterium *: cell :conta ...
... material in all the alive beings? We wants to extract from the DNA of different cellular species such as: - Plant species : the onion - Animal species eukaryote* pluricellular : saliva - Animal species eukaryote* pluricellular : liver of mouse - Animal species prokaryote** : bacterium *: cell :conta ...
Final Study Guide
... 15. What fraction of this cross above will be recessive for both traits? 16. Which one of the following nucleotide pair bonds would be found in a DNA molecule? 17. The backbone of a DNA molecule is made of which two components? 18. Ribosomes are made of _____. 19. Watson and Crick were the first to ...
... 15. What fraction of this cross above will be recessive for both traits? 16. Which one of the following nucleotide pair bonds would be found in a DNA molecule? 17. The backbone of a DNA molecule is made of which two components? 18. Ribosomes are made of _____. 19. Watson and Crick were the first to ...
biochem notes
... • Covalent bond linking two amino acids • A condensation reaction (water is formed and released) • Long chains of amino acids has positive and negative regions which fold to give protein molecules unique shapes • The shapes can be denatured when heated ...
... • Covalent bond linking two amino acids • A condensation reaction (water is formed and released) • Long chains of amino acids has positive and negative regions which fold to give protein molecules unique shapes • The shapes can be denatured when heated ...
1. DNA Extraction from a tomato
... Please open the file “Protocol_DNA_Extraction_from_a_tomato.pdf” and follow the protocol carefully. You‟ll find this protocol on the website just next to this file. Expected answer: Take a picture of each important step during the experiment. Make sure that you take a picture of the DNA you extracte ...
... Please open the file “Protocol_DNA_Extraction_from_a_tomato.pdf” and follow the protocol carefully. You‟ll find this protocol on the website just next to this file. Expected answer: Take a picture of each important step during the experiment. Make sure that you take a picture of the DNA you extracte ...
Taxonomy
... phenotypic and phylogenetic analysis • Used for determining the genus* and species of a newly discovered (micro-)organism ...
... phenotypic and phylogenetic analysis • Used for determining the genus* and species of a newly discovered (micro-)organism ...
Name
... a. Teosinte plants with the desired traits were hybridized until the desired traits appeared, then the offspring were inbred. b. Teosinte plants were randomly bred until the desired traits appeared, then the offspring were hybridized. c. Teosinte plants were hybridized with corn plants, and the resu ...
... a. Teosinte plants with the desired traits were hybridized until the desired traits appeared, then the offspring were inbred. b. Teosinte plants were randomly bred until the desired traits appeared, then the offspring were hybridized. c. Teosinte plants were hybridized with corn plants, and the resu ...
mutations
... (a) The parent strand should be more methylated than the newly synthesized daughter strand (2) In mismatch repair systems, the base to be removed is taken from the under methylated strand 2. If the wrong base is added and is not corrected (or is corrected improperly) before the next replicative cycl ...
... (a) The parent strand should be more methylated than the newly synthesized daughter strand (2) In mismatch repair systems, the base to be removed is taken from the under methylated strand 2. If the wrong base is added and is not corrected (or is corrected improperly) before the next replicative cycl ...
Biotech Mini-Lab Students will model the process of using restriction
... 1. Cut out the plasmid strips along the dotted lines. Connect the strips and tape them together to form a single long strip. Letters should all be in the same direction when the strips are taped. The two ends of the strip should then be taped together with the genetic code facing out to form a circu ...
... 1. Cut out the plasmid strips along the dotted lines. Connect the strips and tape them together to form a single long strip. Letters should all be in the same direction when the strips are taped. The two ends of the strip should then be taped together with the genetic code facing out to form a circu ...
Issues in Biotechnology
... phosphates (red circles) that form the sugar-phosphate backbone of a DNA molecule have a negative charge. ...
... phosphates (red circles) that form the sugar-phosphate backbone of a DNA molecule have a negative charge. ...
Microbial Physiology
... Ammonium (NH4+) is used as the sole N source by most microorganisms. Ammonium could be produced from N2 by nitrogen fixation, or from reduction of nitrate and nitrite. ...
... Ammonium (NH4+) is used as the sole N source by most microorganisms. Ammonium could be produced from N2 by nitrogen fixation, or from reduction of nitrate and nitrite. ...
Lecture_3_2005
... • Developed by Frederick Sanger (for which he won his second Nobel Prize in 1980). ...
... • Developed by Frederick Sanger (for which he won his second Nobel Prize in 1980). ...
F - cell
... Ammonium (NH4+) is used as the sole N source by most microorganisms. Ammonium could be produced from N2 by nitrogen fixation, or from reduction of nitrate and nitrite. ...
... Ammonium (NH4+) is used as the sole N source by most microorganisms. Ammonium could be produced from N2 by nitrogen fixation, or from reduction of nitrate and nitrite. ...
Biochemistry
... 1. The stomach has what optimal pH for that enzyme? 2. What is the optimal pH for the enzyme found in your intestine? 3. What organic substance are both of these enzymes digesting? ...
... 1. The stomach has what optimal pH for that enzyme? 2. What is the optimal pH for the enzyme found in your intestine? 3. What organic substance are both of these enzymes digesting? ...
DNA Extraction Lab
... Analysis A) Look at your description of the DNA that you extracted. If DNA is a rigid structure, why do the strands appear flexible? What features of DNA’s structure account for its rigidity? B) What chemical properties of DNA does this lab make use of? C) Compare the amount of DNA extracted from th ...
... Analysis A) Look at your description of the DNA that you extracted. If DNA is a rigid structure, why do the strands appear flexible? What features of DNA’s structure account for its rigidity? B) What chemical properties of DNA does this lab make use of? C) Compare the amount of DNA extracted from th ...
video slide
... • The nitrogenous bases in DNA – Form hydrogen bonds in a complementary fashion (A with T only, and C with G only) ...
... • The nitrogenous bases in DNA – Form hydrogen bonds in a complementary fashion (A with T only, and C with G only) ...
BIO 402/502 Advanced Cell & Developmental Biology
... genomes with respect to size, percent of coding region and number of genes ...
... genomes with respect to size, percent of coding region and number of genes ...
Genes - University of Arizona | Ecology and Evolutionary Biology
... DNA Technology The following are some of the most important molecular methods we will be using in this course. They will be used, among other things, for ...
... DNA Technology The following are some of the most important molecular methods we will be using in this course. They will be used, among other things, for ...
Nerve activates contraction - Jackson County School District
... • Silences (same as above, just turn gene off) • Methylation of DNA • mRNA processing before it leaves the nucleus • Gene regulation in Bacteria: • The Operon- A group, or cluster, of genes (that make work together) or a segment of DNA that functions as a single transcription unit. It is comprised o ...
... • Silences (same as above, just turn gene off) • Methylation of DNA • mRNA processing before it leaves the nucleus • Gene regulation in Bacteria: • The Operon- A group, or cluster, of genes (that make work together) or a segment of DNA that functions as a single transcription unit. It is comprised o ...
CHAPTER 14: DNA: THE GENETIC MATERIAL
... c. Nitrogen containing base: Purine or pyrimidine 1) Purines = adenine (A), guanine (G) 2) Pyrimidines = thymine (T), cytosine (C), RNA contains uracil (U) not T 3. DNA and RNA composed of repeating units a. Called nucleotides b. Nitrogen base distinguishes nucleotide identity 4. Numbering scheme fo ...
... c. Nitrogen containing base: Purine or pyrimidine 1) Purines = adenine (A), guanine (G) 2) Pyrimidines = thymine (T), cytosine (C), RNA contains uracil (U) not T 3. DNA and RNA composed of repeating units a. Called nucleotides b. Nitrogen base distinguishes nucleotide identity 4. Numbering scheme fo ...
Nucleic acid analogue
Nucleic acid analogues are compounds which are analogous (structurally similar) to naturally occurring RNA and DNA, used in medicine and in molecular biology research.Nucleic acids are chains of nucleotides, which are composed of three parts: a phosphate backbone, a pucker-shaped pentose sugar, either ribose or deoxyribose, and one of four nucleobases.An analogue may have any of these altered. Typically the analogue nucleobases confer, among other things, different base pairing and base stacking properties. Examples include universal bases, which can pair with all four canonical bases, and phosphate-sugar backbone analogues such as PNA, which affect the properties of the chain (PNA can even form a triple helix).Nucleic acid analogues are also called Xeno Nucleic Acid and represent one of the main pillars of xenobiology, the design of new-to-nature forms of life based on alternative biochemistries.Artificial nucleic acids include peptide nucleic acid (PNA), Morpholino and locked nucleic acid (LNA), as well as glycol nucleic acid (GNA) and threose nucleic acid (TNA). Each of these is distinguished from naturally occurring DNA or RNA by changes to the backbone of the molecule.In May 2014, researchers announced that they had successfully introduced two new artificial nucleotides into bacterial DNA, and by including individual artificial nucleotides in the culture media, were able to passage the bacteria 24 times; they did not create mRNA or proteins able to use the artificial nucleotides. The artificial nucleotides featured 2 fused aromatic rings.