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Chapter 12 Notes - Great Neck Public Schools
Chapter 12 Notes - Great Neck Public Schools

... a) Often require these organisms to be genetically altered such that they would not be able to survive outside the lab. b) Forbidden to work with human cancer genes or genes of extremely virulent pathogens. 2. Controversy have erupted nevertheless ...
An enlarged largest subunit or Plasmodium falciparum RNA
An enlarged largest subunit or Plasmodium falciparum RNA

... along with the presence of the heptapeptide repeat in the CTD of the P. falciparum protein (Fig. 3.), shows that the gene we characterized is a form of the P. falciparum RPII subunit. The homologous sequences of the P. falciparum RPII subunit and the other RPII subunits were partitioned into only 5 ...
Mutations in Splice Sites
Mutations in Splice Sites

... • For those amino acids having more than one codon, the first two bases in the codon are usually the same. The base in the third position often varies. • The code is almost universal (the same in all organisms). Some minor exceptions to this occur in mitochondria and some organisms. • The code is co ...
Chapter 13 Genetic Engineering, TE
Chapter 13 Genetic Engineering, TE

... When you read about related concepts, a compare-and-contrast table can help you focus on their similarities and differences. Construct a table to compare and contrast transformation in bacteria, plants, and animals. Look in Appendix A for more information about compare-and-contrast tables. Do your w ...
Choose the BEST answer! Two points each. 1. Which of the
Choose the BEST answer! Two points each. 1. Which of the

... sensitivity in the host cell. d. The host cell's tetracycline resistance is not likely to be affected by the new DNA e. The E. coli will now be only partially sensitive to tetracycline. Use the following information to answer #25 - 28 You have four reaction flasks in which you have provided all the ...
Protein synthesis
Protein synthesis

... o In E. coli, a purine-rich sequence of nucleotide bases, is located six to ten bases upstream of the initiating AUG codon on the mRNA molecule—that is, near its 5'-end. o Eukaryotic messages do not have SD sequences. o In eukaryotes, the 40S ribosomal subunit binds close to the cap structure at the ...
Exam Review 2 10/2/16
Exam Review 2 10/2/16

... A. The Y-shaped region where the DNA is split into two separate strands for coding B. Growing as DNA replication proceeds because synthesis is bidirectional C. The location at which the replication process begins D. Present only in bacterial cells and not in eukaryotes 45. Reverse transcriptase is c ...
分子生物學小考(一) 範圍ch3~ch7
分子生物學小考(一) 範圍ch3~ch7

... 4. In prokaryotes, environmental sensing frequently involves regulatory proteins (two-component system) that sense and respond to changes in surroundings. These two-component systems may involve which of the following? I. Protein phosphorylation (A) I only ...
Slides - Department of Computer Science • NJIT
Slides - Department of Computer Science • NJIT

... consisting of four letters: A, C, G, and T. They could be very long, e.g. thousands and even millions of letters • Proteins are also represented as strings of 20 letters (each letter is an amino acid). Their 3-D structure determines the function to a large extent. ...
國立彰化師範大學九十六學年度碩士班招生考試試題
國立彰化師範大學九十六學年度碩士班招生考試試題

... 8. _____: The bacterial RNA polymerase consists of a core enzyme (α2ββ’) and a sigma factor (σ). If we treated the enzyme with an antibiotic (rifamycin), the activity of a subunit β was inhibited by preventing initiation of transcription, prior to the formation of the first phospodiester bond. So th ...
Mitosis
Mitosis

... They both are autotrophs and produce carbohydrates 4. The organism which can not make their own food are known as heterotrophs. 5. What does a heterotrophic organism need to do in order to get the energy? Consume other organisms 6. A decomposer that obtains nutrients by breaking down dead and decayi ...
Detection of Beer Spoilage Organisms by Polymerase
Detection of Beer Spoilage Organisms by Polymerase

... beer market has made the biological monitoring of beer spoilage microorganisms even more important. The plate count method for enumerating microbiological contamination has remained unchanged for over a century, but it requires several days before the microorganisms are detected. Many rapid detectio ...
Mitosis
Mitosis

... They both are autotrophs and produce carbohydrates 4. The organism which can not make their own food are known as heterotrophs. 5. What does a heterotrophic organism need to do in order to get the energy? Consume other organisms 6. A decomposer that obtains nutrients by breaking down dead and decayi ...
Protein Synthesis
Protein Synthesis

... mRNA Processing: The Movie from the “Virtual Cell Animation collection: molecular and Cellular biology http://vcell.ndsu.edu/animations/mrnaprocessing/movie-flash.htm ...
Unit 6: Biotechnology
Unit 6: Biotechnology

... 1. Plasmids or viruses are then used as a vector, a genetic vehicle that carries foreign DNA into a host cell. Usually, the host cell is a ____Bacterium___. 2. The recombinant DNA inside the host cell reproduces new cells that contain copies of the inserted gene. F. Screening – extracting copied gen ...
Bacterial Transformation with Recombinant DNA
Bacterial Transformation with Recombinant DNA

... cosmids. In this lab we will use plasmid vectors. Plasmids Plasmids are small circular DNA molecules found in bacteria. They replicate independently of the bacterial chromosome and depending on the plasmid there may be from 1 to over a hundred copies per cell. If a given plasmid used for cloning is ...
RNA interference was popularized by work in C
RNA interference was popularized by work in C

... for DICER activity, which generate more siRNAs. In some organisms with endogenous RNAi mechanisms, for example, fungi, plants worms and mammals, RNAi also involve another amplification step. In this step, single-stranded siRNAs not associated with RISC bind to their target ...
Topic Definition 3` Refers to the third carbon of the nucleic acid
Topic Definition 3` Refers to the third carbon of the nucleic acid

... a bacterial origin of replication which maintains one copy of the BAC per cell. Although formally incorrect (the nitrogenous base which gives each nucleotide its name is only part of the nucleotide), this is often used as a synonym for "nucleotide." The hydrogen bonding of one of the bases (A, C, G, ...
Poster
Poster

... yHst2 is the yeast homologue of human Sir two 2. All Sir2 deacetylases have amino acid sequences that are very similar in all organisms from bacteria to humans. They all remove acetyl groups from acetyllysine sidechains on the proteins that they target. They all use NAD+ to accomplish this. Sir2 pro ...
Predicting TF affinities to Promoters of tissue specific genes
Predicting TF affinities to Promoters of tissue specific genes

... acid sequence (DNA). DNA is a linear biopolymer composed of four different subunits or bases called adenine (A), cytosine (C), guanine (G) and thymine (T). Typically millions of these bases are connected via a sugar phosphate backbone to form individual eukaryotic chromosomes (Lodish et al., 1995). ...
microbiology exam i - Medical Mastermind Community
microbiology exam i - Medical Mastermind Community

... A. They contain Ira genes that mediate self transmissibility. B. They contain an origin of replication. C. They are present in very low copy numbers. D. Depending on the plasmid, they can confer resistance to more than one antibiotic. E. All of the above. 25. Mutations in the human gene that is resp ...
Supplementary Information (doc 36K)
Supplementary Information (doc 36K)

... centrifuged for 60 min at 15230 × g and the supernatant discarded. The pellet was washed twice by shaking the tube with the pellet and with 600 μl of 80% ethanol for 15 s, centrifuging for 5 min at maximum speed (17135 × g), and discarding the supernatant each time. The pellet in the tube was then ...
We report on a system developed by Bio-Rad
We report on a system developed by Bio-Rad

HONORS BIOLOGY FINAL EXAM STUDY GUIDE 2015
HONORS BIOLOGY FINAL EXAM STUDY GUIDE 2015

... 5. If an organism has 80 chromosomes, how would non-disjunction affect the resulting daughter cells during meiosis? Provide a specific example of a human genetic disorder resulting from non-disjunction. 6. Ms. Tung cuts her big toe. What cellular division process would be used to repair the damage? ...
plotfold
plotfold

... In Nucleic acids, inverted repeat sequences may indicate foldback (self pairing)structures. ...
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Nucleic acid analogue



Nucleic acid analogues are compounds which are analogous (structurally similar) to naturally occurring RNA and DNA, used in medicine and in molecular biology research.Nucleic acids are chains of nucleotides, which are composed of three parts: a phosphate backbone, a pucker-shaped pentose sugar, either ribose or deoxyribose, and one of four nucleobases.An analogue may have any of these altered. Typically the analogue nucleobases confer, among other things, different base pairing and base stacking properties. Examples include universal bases, which can pair with all four canonical bases, and phosphate-sugar backbone analogues such as PNA, which affect the properties of the chain (PNA can even form a triple helix).Nucleic acid analogues are also called Xeno Nucleic Acid and represent one of the main pillars of xenobiology, the design of new-to-nature forms of life based on alternative biochemistries.Artificial nucleic acids include peptide nucleic acid (PNA), Morpholino and locked nucleic acid (LNA), as well as glycol nucleic acid (GNA) and threose nucleic acid (TNA). Each of these is distinguished from naturally occurring DNA or RNA by changes to the backbone of the molecule.In May 2014, researchers announced that they had successfully introduced two new artificial nucleotides into bacterial DNA, and by including individual artificial nucleotides in the culture media, were able to passage the bacteria 24 times; they did not create mRNA or proteins able to use the artificial nucleotides. The artificial nucleotides featured 2 fused aromatic rings.
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