Chapter 2
... chains of amino acids (20 different kinds) Shape of protein is determined by how amino acids react with on another and with water Enzymes are proteins that promote chemical reactions Also important for structural functions and for the immune system ...
... chains of amino acids (20 different kinds) Shape of protein is determined by how amino acids react with on another and with water Enzymes are proteins that promote chemical reactions Also important for structural functions and for the immune system ...
Lab 7: Molecular Biology
... DNA. In bacteria, the chromosomes are circular, as are the DNA molecules contained in these chromosomes. Although these circular DNA molecules are usually broken into linear fragments during purification, some bacteria also possess smaller extrachromosomal circular DNA molecules that are easier to i ...
... DNA. In bacteria, the chromosomes are circular, as are the DNA molecules contained in these chromosomes. Although these circular DNA molecules are usually broken into linear fragments during purification, some bacteria also possess smaller extrachromosomal circular DNA molecules that are easier to i ...
Ch. 17 DNA mutations and Repair
... Terms associated with PHENOTYPIC effects of mutations on protein structure ...
... Terms associated with PHENOTYPIC effects of mutations on protein structure ...
Quantitative RT-PCR
... without input of any templates. 7. Fractionation of Amplified Wild Type and Control Templates a. Load 5 ml of RT-PCR sample on 2% agarose gel. b. According to the signal intensities in agarose gel, determine the relative amount of different samples to be used for fragmentation to obtain similar inte ...
... without input of any templates. 7. Fractionation of Amplified Wild Type and Control Templates a. Load 5 ml of RT-PCR sample on 2% agarose gel. b. According to the signal intensities in agarose gel, determine the relative amount of different samples to be used for fragmentation to obtain similar inte ...
Effect of Acid Base on different Brands of Sitagliptin by using UV
... 200ppm solution of different brands of Sitagliptin (Sita, Trevia, Tagip). To determine the effect of acid and base on different brands of Sitagliptin (Sita, Trevia, Tagip) the 200 ppm solution of each brand was transferred in to two separate test tubes that contain 5ml of acid HCl and base NaOH sepa ...
... 200ppm solution of different brands of Sitagliptin (Sita, Trevia, Tagip). To determine the effect of acid and base on different brands of Sitagliptin (Sita, Trevia, Tagip) the 200 ppm solution of each brand was transferred in to two separate test tubes that contain 5ml of acid HCl and base NaOH sepa ...
Nucleotide Metabolism Nucleotide sources - Rose
... In humans, dietary nucleotide bases are rarely incorporated into nucleotides. As a result, humans must synthesize their own nucleotide bases. (With the exception of a few parasitic prokaryotes, all organisms can synthesize nucleotides.) Although all nucleated eukaryotic cells can synthesize nucleoti ...
... In humans, dietary nucleotide bases are rarely incorporated into nucleotides. As a result, humans must synthesize their own nucleotide bases. (With the exception of a few parasitic prokaryotes, all organisms can synthesize nucleotides.) Although all nucleated eukaryotic cells can synthesize nucleoti ...
Slide 1
... Microsatellites (SSR) AFLP (Amplified Fragment Length Polymorphism) RAPD (random amplified polymorphic DNA) ...
... Microsatellites (SSR) AFLP (Amplified Fragment Length Polymorphism) RAPD (random amplified polymorphic DNA) ...
Recombinant DNA - Rose
... heterologous DNA. An expression plasmid must therefore have, in addition to the features listed above, a strong promoter element that will drive transcription of the foreign DNA1 in the host organism, and an effective ribosome binding site that will allow efficient translation of the transcribed RNA ...
... heterologous DNA. An expression plasmid must therefore have, in addition to the features listed above, a strong promoter element that will drive transcription of the foreign DNA1 in the host organism, and an effective ribosome binding site that will allow efficient translation of the transcribed RNA ...
Lecture 18: Powerpoint
... The catalytic site on the large subunit catalyzes the formation of a peptide bond linking the amino acids ...
... The catalytic site on the large subunit catalyzes the formation of a peptide bond linking the amino acids ...
PowerPoint 演示文稿
... Nucleic acid probe a strand of nucleic acid that can be labeled and used to hybridize to a complementary molecule from mixture of other nucleic acids Phenotype the observable characteristics of an organism Plasmid an extrachromosomal genetic element that has no extracellular form Point mutation a m ...
... Nucleic acid probe a strand of nucleic acid that can be labeled and used to hybridize to a complementary molecule from mixture of other nucleic acids Phenotype the observable characteristics of an organism Plasmid an extrachromosomal genetic element that has no extracellular form Point mutation a m ...
Review Questions
... ribosome is the site of protein synthesis in a cell. A ribosome has two parts called subunits. When not translating, the small subunit is unattached to the large subunit. During translation, the small subunit will bond to the mRNA and the large subunit bonds to the small subunit cradling the mRNA be ...
... ribosome is the site of protein synthesis in a cell. A ribosome has two parts called subunits. When not translating, the small subunit is unattached to the large subunit. During translation, the small subunit will bond to the mRNA and the large subunit bonds to the small subunit cradling the mRNA be ...
Ch. 1 Plasmids
... c. Bacterial transformation is the process by which bacterial cells take up naked DNA molecules (Fig 1-2). If the foreign DNA, such as a plasmid, has an origin of replication recognized by the host cell DNA polymerases, the bacteria will replicate the foreign DNA along with their own DNA. Transforma ...
... c. Bacterial transformation is the process by which bacterial cells take up naked DNA molecules (Fig 1-2). If the foreign DNA, such as a plasmid, has an origin of replication recognized by the host cell DNA polymerases, the bacteria will replicate the foreign DNA along with their own DNA. Transforma ...
Genetically Modified Organisms - Lightweight OCW University of
... • Deoxyribonucleic acid, any of various nucleic acids that are usually the molecular basis of heredity, are located in the nuclei of cells, and are constructed of a double helix held together by hydrogen bonds. • If placed end-to-end, DNA strands would stretch more than five feet but would be only 5 ...
... • Deoxyribonucleic acid, any of various nucleic acids that are usually the molecular basis of heredity, are located in the nuclei of cells, and are constructed of a double helix held together by hydrogen bonds. • If placed end-to-end, DNA strands would stretch more than five feet but would be only 5 ...
11_Lecture_Presentation
... information flow from genes to proteins – Mainly controlled at the level of transcription – A gene that is “turned on” is being transcribed to produce mRNA that is translated to make its corresponding protein – Organisms respond to environmental changes by controlling gene expression ...
... information flow from genes to proteins – Mainly controlled at the level of transcription – A gene that is “turned on” is being transcribed to produce mRNA that is translated to make its corresponding protein – Organisms respond to environmental changes by controlling gene expression ...
Document
... Cell division is essential for an organism to grow, but, when a cell divides, it must replicate the DNA in its genome so that the two daughter cells have the same genetic information as their parent. The double-stranded structure of DNA provides a simple mechanism for DNA replication. Here, the two ...
... Cell division is essential for an organism to grow, but, when a cell divides, it must replicate the DNA in its genome so that the two daughter cells have the same genetic information as their parent. The double-stranded structure of DNA provides a simple mechanism for DNA replication. Here, the two ...
Study questions - Pre-lab
... d. What do we mean when we say a SNP is associated with a certain phenotypic trait? We mean that it’s not necessarily causal to the phenotypic trait. The SNP segregates with the trait, but it may or may not be its underlying cause (for example, the SNP DNA may be in very close proximity to the DNA u ...
... d. What do we mean when we say a SNP is associated with a certain phenotypic trait? We mean that it’s not necessarily causal to the phenotypic trait. The SNP segregates with the trait, but it may or may not be its underlying cause (for example, the SNP DNA may be in very close proximity to the DNA u ...
Chromosome - s3.amazonaws.com
... Sequence of 3 bases on DNA or ‘triplet’ that codes for a particular amino acid. Gene (s) Genes A section of DNA that contains coded information as a sequence of bases. Genes code for polypeptides (small proteins). It is these polypeptides that determine the nature and development of organisms. Allel ...
... Sequence of 3 bases on DNA or ‘triplet’ that codes for a particular amino acid. Gene (s) Genes A section of DNA that contains coded information as a sequence of bases. Genes code for polypeptides (small proteins). It is these polypeptides that determine the nature and development of organisms. Allel ...
An Artist in Gene Editing - Max-Planck
... CRISPR-Cas9: CRISPR stands for “Clustered Regularly Interspaced Short Palindromic Repeats” and describes a genome sequence in bacteria. Cas9 is an endonuclease – an enzyme that cuts DNA. In viral infections, the bacteria cut sequences out of the viral genome and insert them into the CRISPR sequence. ...
... CRISPR-Cas9: CRISPR stands for “Clustered Regularly Interspaced Short Palindromic Repeats” and describes a genome sequence in bacteria. Cas9 is an endonuclease – an enzyme that cuts DNA. In viral infections, the bacteria cut sequences out of the viral genome and insert them into the CRISPR sequence. ...
Reactive Oxygen Species
... “Premarin (Wyeth–Ayerst) is the most common drug used for hormone replacement therapy (HRT) and is composed of approximately 50% estrogens and 40% equine estrogens [equilenin (EN) and equilin (EQ)] (9). In vitro experiments have shown that equine estrogens are successively metabolized and are capabl ...
... “Premarin (Wyeth–Ayerst) is the most common drug used for hormone replacement therapy (HRT) and is composed of approximately 50% estrogens and 40% equine estrogens [equilenin (EN) and equilin (EQ)] (9). In vitro experiments have shown that equine estrogens are successively metabolized and are capabl ...
Document
... (12) What is the purpose of the biotin and the fluorescent molecules? What happens when the RNA sample is washed over the microarray? ...
... (12) What is the purpose of the biotin and the fluorescent molecules? What happens when the RNA sample is washed over the microarray? ...
life sciences p2
... this portion of nucleic acid will code for a chain of eight amino acids. the sequence given will be complementary to the sequence C T C G T G C T T. the nucleic acid shown contains the sugar ribose. the nucleic acid shown is DNA. ...
... this portion of nucleic acid will code for a chain of eight amino acids. the sequence given will be complementary to the sequence C T C G T G C T T. the nucleic acid shown contains the sugar ribose. the nucleic acid shown is DNA. ...
Macromolecules: Proteins
... Color code the amino acid on this worksheet (carbon-black, hydrogen-yellow, nitrogen-blue, and oxygen-red). Basic Structure of Amino acid H ...
... Color code the amino acid on this worksheet (carbon-black, hydrogen-yellow, nitrogen-blue, and oxygen-red). Basic Structure of Amino acid H ...
Data Acquisition Tools & Techniques
... • In the technique, researchers squirt a solution of cell contents onto a narrow polymer strip that has a gradient of acidity. When the strip is exposed to an electric current, each protein in the mixture settles into a layer according to its charge. Next, the strip is placed along the edge of a fla ...
... • In the technique, researchers squirt a solution of cell contents onto a narrow polymer strip that has a gradient of acidity. When the strip is exposed to an electric current, each protein in the mixture settles into a layer according to its charge. Next, the strip is placed along the edge of a fla ...
Nucleic acid analogue
Nucleic acid analogues are compounds which are analogous (structurally similar) to naturally occurring RNA and DNA, used in medicine and in molecular biology research.Nucleic acids are chains of nucleotides, which are composed of three parts: a phosphate backbone, a pucker-shaped pentose sugar, either ribose or deoxyribose, and one of four nucleobases.An analogue may have any of these altered. Typically the analogue nucleobases confer, among other things, different base pairing and base stacking properties. Examples include universal bases, which can pair with all four canonical bases, and phosphate-sugar backbone analogues such as PNA, which affect the properties of the chain (PNA can even form a triple helix).Nucleic acid analogues are also called Xeno Nucleic Acid and represent one of the main pillars of xenobiology, the design of new-to-nature forms of life based on alternative biochemistries.Artificial nucleic acids include peptide nucleic acid (PNA), Morpholino and locked nucleic acid (LNA), as well as glycol nucleic acid (GNA) and threose nucleic acid (TNA). Each of these is distinguished from naturally occurring DNA or RNA by changes to the backbone of the molecule.In May 2014, researchers announced that they had successfully introduced two new artificial nucleotides into bacterial DNA, and by including individual artificial nucleotides in the culture media, were able to passage the bacteria 24 times; they did not create mRNA or proteins able to use the artificial nucleotides. The artificial nucleotides featured 2 fused aromatic rings.