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Using DNA Barcoding to Identify Freshwater Algae in Two Bodies of
Using DNA Barcoding to Identify Freshwater Algae in Two Bodies of

... northern most body of water and is connected to Athasca Lake by a small and narrow channel (Figure 1). The presence of certain organisms can indicate the water quality by their ability to tolerate chemicals or conditions (Dokulil, M.T., 2003), which are called "bioindicators." Our goal was to isolat ...
click here
click here

... heterozygoes; the child has inherited a mutant allele from one parent, and a wild type allele from the other parent, so the fetus is also a heterozygote: The fetus, therefore is phenotypically normal, but can pass the disease allele on to his progeny. Within the general population, however, the freq ...
video slide - SharpSchool
video slide - SharpSchool

... A A G Anticodon (a) Two-dimensional structure. The four base-paired regions and three loops are characteristic of all tRNAs, as is the base sequence of the amino acid attachment site at the 3 end. The anticodon triplet is unique to each tRNA type. (The asterisks mark bases that have been chemically ...
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... Termination of transcription in prokaryotes 1) Sometimes go until ribosomes fall too far behind 2) ~50% of E.coli genes require a termination factor called “rho” ...
Disease name
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... field samples revealed that 32% (6 of 19 batches) of PCR-positive cases had nervous lesions according to the histopathology examination. All PCR-negative batches have unidentified histopathological lesions, and no virions were observed via the electron microscopic examination using direct negative s ...
Unsuitability of Using Ribosomal RNA as Loading Control for
Unsuitability of Using Ribosomal RNA as Loading Control for

... problems such as uneven loading or different efficiency of transfer between lanes that may cause errors when verifying changes in gene expression. One of the most popular approaches to solving them is the normalization of the gene of interest to a housekeeping gene, such as glyceraldehyde-3-phosphat ...
PDS 803482 Ron Blood and Cell DNA Mini
PDS 803482 Ron Blood and Cell DNA Mini

... Ron’s Blood and Cell DNA Mini Kit is designed for quick preparation of pure DNA. The kit contains spin columns, buffers and reagents necessary for cell lysis, DNA binding, washing and elution of DNA into small volume. Each kit contains a manual with detailed protocols of DNA extraction and purificat ...
as PDF
as PDF

... Fig. 3. Schematic illustration of a typical horizontal gel electrophoresis setup for the separation of nucleic acids. The two buffers vary according to the advantages and disadvantages. For instance, Borate has disadvantages as it polymerizes and/or interacts with cis diols found in RNA. TAE on the ...
viruses - biology3u
viruses - biology3u

...  Also contain RNA as genetic material vs. DNA  When a virus infects a cell, it makes a copy of _____________ from the viral RNA code  Remember, _________ genetic transfer is from DNA to RNA but these viruses ________ the direction of genetic information transfer from normal to ‘_____________’  i ...
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... amino acid sequence. – The correct amino acid sequence is determined by the cell’s genetic information. – The slightest change in this sequence may affect the protein’s ability to function. ...
Nitrogen Cycle
Nitrogen Cycle

Build a Paper Model of Transfer RNA (tRNA)
Build a Paper Model of Transfer RNA (tRNA)

... double line (16 total). Be careful not to cut through the entire strip. ...
This exam has 8 pages, including this one.
This exam has 8 pages, including this one.

... ii) Draw the dipeptide in an extended chain conformation that would result from forming a peptide bond between these two amino acids; you need not draw the sidechains (just label them R1 and R2) but you need to draw all mainchain atoms. (4 pts) ...
cinnabar - UWL faculty websites
cinnabar - UWL faculty websites

Protein Buffer Systems
Protein Buffer Systems

... • Haemoglobin is quantitatively about 6 times more important then the plasma proteins as it is present in about twice the concentration and contains about three times the number of histidine residues per molecule. For example if blood pH changed from 7.5 to 6.5, haemoglobin would buffer 27.5 mmol/l ...
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Preparation of MyoD mRNA for the differentiation of stem cells into

... Stem cells have been extensively studied by scientists as they have the ability to differentiate into different somatic cells, or diploid cells, and carry out different functions. However, somatic cells could also be induced to differentiate back into stem cells and then be differentiated into other ...
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Agarose gel reagents and buffers - Scie-Plas

... pore size of an agarose gel is inversely dependent on the agarose concentration. The pores in agarose gels are generally much larger than those in acrylamide gels, making them suitable for the separation of much larger nucleic acid fragments. There are many types of agarose available. The best choic ...
Chapter 7: The New Genetics—Techniques for DNA Analysis
Chapter 7: The New Genetics—Techniques for DNA Analysis

... The DNA of an individual—I will use myself as the example—is then purified and the bonds connecting the two strands of the DNA molecule are cut, making the DNA single stranded. I happen to be a heterozygote at the locus at which the probe will bind. The difference in the alleles is subtle, but it ap ...
Keystone Review Packet #2 Answers
Keystone Review Packet #2 Answers

Chapter 12
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... You should now be able to 6. Explain how different organisms are used to massproduce proteins of human interest. 7. Explain how DNA technology has helped to produce insulin, growth hormone, and vaccines. 8. Explain how genetically modified (GM) organisms are transforming agriculture. 9. Describe th ...
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... introduction of Cu to the soil. Here the incoporation of tritiated bromodeoxyuridine (BrdU) is tested as a cultivation independent measure for the effect of Cu exposure on the bacterial tolerance to Cu and antibiotics. ...
Capryloyl Salicylic Acid Technical Data Sheet
Capryloyl Salicylic Acid Technical Data Sheet

... Capryloyl Salicylic Acid is an exfoliant, anti-inflammatory and antiseptic. It’s used to treat the appearance of dry damaged skin, reducing dry patches/flaking and restoring suppleness to the skin. This means that they not only exfoliate the upper layer of skin, but also penetrate deep through the e ...
Chapter 2 - Biochemistry
Chapter 2 - Biochemistry

... • There are a large number of different types of proteins: – The number, kind and sequence of amino acids lead to this large variety ...
Video Clip: Supersize Me in 7 Minutes
Video Clip: Supersize Me in 7 Minutes

... Clean the test tubes and then add Egbert’s stomach solution to 4 test tubes. 1. To test Eggbert’s stomach contents for starch, place a drop of iodine solution in the test tube. Observe any color change. Note the color change in the data table. 2. To test Eggbert’s stomach contents for sugar, add sev ...
Document
Document

... Weak bonds also occur throughout the polypeptide, between the amino acid side chains in regions of secondary structure as well as the looped regions ...
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Nucleic acid analogue



Nucleic acid analogues are compounds which are analogous (structurally similar) to naturally occurring RNA and DNA, used in medicine and in molecular biology research.Nucleic acids are chains of nucleotides, which are composed of three parts: a phosphate backbone, a pucker-shaped pentose sugar, either ribose or deoxyribose, and one of four nucleobases.An analogue may have any of these altered. Typically the analogue nucleobases confer, among other things, different base pairing and base stacking properties. Examples include universal bases, which can pair with all four canonical bases, and phosphate-sugar backbone analogues such as PNA, which affect the properties of the chain (PNA can even form a triple helix).Nucleic acid analogues are also called Xeno Nucleic Acid and represent one of the main pillars of xenobiology, the design of new-to-nature forms of life based on alternative biochemistries.Artificial nucleic acids include peptide nucleic acid (PNA), Morpholino and locked nucleic acid (LNA), as well as glycol nucleic acid (GNA) and threose nucleic acid (TNA). Each of these is distinguished from naturally occurring DNA or RNA by changes to the backbone of the molecule.In May 2014, researchers announced that they had successfully introduced two new artificial nucleotides into bacterial DNA, and by including individual artificial nucleotides in the culture media, were able to passage the bacteria 24 times; they did not create mRNA or proteins able to use the artificial nucleotides. The artificial nucleotides featured 2 fused aromatic rings.
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