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Synthesis of Oligonucleotides
Synthesis of Oligonucleotides

... chosen, since they are stable for long periods during mildly basic and acidic conditions used during oligonucleotide synthesis, and are removed with concentrated ammonia at the end of the synthesis (Section 4.1.4). The benzoyl group is used to protect both adenine and cytosine, while isobutyryl is u ...
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Fast, simultaneous, and sensitive detection of staphylococci
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Practice test 2
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... c. separate DNA fragments by charge and length d. inject foreign DNA into animal and plant cells 4. Recombinant DNA are currently used to produce _____. a. clothing dye, cheese, and laundry products b. human antibodies and vaccines c. crops that test better and stay fresh longer d. all of these 5. A ...
Amino Acid Catabolism - Chemistry Courses: About
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Publication JournalArticle (Originalarbeit in einer wissenschaftlichen
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... Gamma-aminobutyric acid aminotransferase (GABA-AT) is a pyridoxal 5'-phosphate-dependent enzyme responsible for the degradation of the inhibitory neurotransmitter GABA. GABA-AT is a validated target for antiepilepsy drugs because its selective inhibition raises GABA concentrations in brain. The anti ...
Amino Acid Catabolism - Chemistry Courses: About
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Introduction to the GCG Wisconsin Package
Introduction to the GCG Wisconsin Package

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Introduction to the GCG Wisconsin Package
Introduction to the GCG Wisconsin Package

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Chemdraw B&W - Pennsylvania State University
Chemdraw B&W - Pennsylvania State University

... • Only the pyridine-like, doubly bonded nitrogen in histidine is basic. The pyrrole-like singly bonded nitrogen is nonbasic because its lone pair of electrons is part of the 6  electron aromatic imidazole ring (see Section 24.4). ...
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Exogenous nucleotides accelerate early replication
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... In order to measure the total amount of newly synthesised DNA, the cells were loaded with 30 nM [α-32P]2′-deoxycytidine 5′triphosphate (3000 Ci mmol−1, ICN Biomedicals, Inc., USA), which was included in the solutions loaded into the cells during the hypotonic shift. Then, the cells were incubated in ...
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... • This can be done by modifying the flux through the PPP, either by increasing the activity of transketolase (and providing more erythrose 4phosphate for aromatic amino acids biosynthesis) or by decreasing the activity of transketolase (and providing more ribose 5phosphate for L-histidine biosynthe ...
Fuel Metabolism PART 1: Structure and Function of Protein
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... 3-C. DNA chains are composed of nucleosides joined by 3',5'-phosphodiester bonds. Each nucleoside consists of a nitrogenous base linked to deoxyribose. Two DNA chains, oriented in opposite directions, base-pair with each other and are twisted to form a double helix. The bases are stacked on top of e ...
Food Biotechnology Dr. Tarek Elbashiti
Food Biotechnology Dr. Tarek Elbashiti

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Nucleic acid analogue



Nucleic acid analogues are compounds which are analogous (structurally similar) to naturally occurring RNA and DNA, used in medicine and in molecular biology research.Nucleic acids are chains of nucleotides, which are composed of three parts: a phosphate backbone, a pucker-shaped pentose sugar, either ribose or deoxyribose, and one of four nucleobases.An analogue may have any of these altered. Typically the analogue nucleobases confer, among other things, different base pairing and base stacking properties. Examples include universal bases, which can pair with all four canonical bases, and phosphate-sugar backbone analogues such as PNA, which affect the properties of the chain (PNA can even form a triple helix).Nucleic acid analogues are also called Xeno Nucleic Acid and represent one of the main pillars of xenobiology, the design of new-to-nature forms of life based on alternative biochemistries.Artificial nucleic acids include peptide nucleic acid (PNA), Morpholino and locked nucleic acid (LNA), as well as glycol nucleic acid (GNA) and threose nucleic acid (TNA). Each of these is distinguished from naturally occurring DNA or RNA by changes to the backbone of the molecule.In May 2014, researchers announced that they had successfully introduced two new artificial nucleotides into bacterial DNA, and by including individual artificial nucleotides in the culture media, were able to passage the bacteria 24 times; they did not create mRNA or proteins able to use the artificial nucleotides. The artificial nucleotides featured 2 fused aromatic rings.
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